Article
Plant Sciences
Ayako Okuzaki, Thilo Ruhle, Dario Leister, Christian Schmitz-Linneweber
Summary: The study reveals that the processing of chloroplast RNA relies on a large number of nuclear-encoded RNA-binding proteins, with the CP31A protein playing a crucial role in chloroplast development under low temperature conditions. The acidic domain of CP31A shows significant differences in function in RNA metabolism and cold resistance, with different functions associated with different regions of the protein.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Le Nguyen Tieu Ngoc, Su Jung Park, Jing Cai, Trinh Thi Huong, Kwanuk Lee, Hunseung Kang
Summary: Ribosomal RNA (rRNA) methylation is crucial for ribosome assembly and function, which are important for plant growth and stress responses. In this study, a new rRNA methyltransferase, RsmD, was identified in Arabidopsis chloroplasts, and it plays a role in plant adaptation to cold stress.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Mingjiu Li, Hannes Ruwe, Michael Melzer, Astrid Junker, Goetz Hensel, Henning Tschiersch, Serena Schwenkert, Sindy Chamas, Christian Schmitz-Linneweber, Thomas Boerner, Nils Stein
Summary: The Arabidopsis gene CIA2 and its paralogous gene CIL encode transcription factors that positively affect the activity of nuclear genes for chloroplast functions and protein import machineries. In addition, it was observed that the CIL gene showed nuclear localization when transiently expressed in Arabidopsis protoplasts.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Lisa-Marie Schmid, Nikolay Manavski, Wei Chi, Jorg Meurer
Summary: The formation of chloroplasts can be traced back to the ingestion of a cyanobacterium by a eukaryotic host cell containing mitochondria. Recent research has explored the maturation of rRNA and ribosome assembly in chloroplasts, along with their crucial role in plant survival and acclimation. Comparison between ancient and modern chloroplasts provides insights into the evolution of ribosome assembly factors, showing the emergence of newly evolved factors to help plants adapt to changing environments and signals.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Monika Zakrzewska-Placzek, Anna Golisz-Mocydlarz, Michal Krzyszton, Justyna Piotrowska, Malgorzata Lichocka, Joanna Kufel
Summary: This study demonstrates the involvement of AtNOL12 in rRNA biogenesis and its correlation with stress response in plants. The absence of AtNOL12 alters the expression of ribosomal protein and ribosome biogenesis genes, potentially impacting ribosome function.
Article
Plant Sciences
Mengmeng Kong, Yaozong Wu, Ziyuan Wang, Wantong Qu, Yixin Lan, Xin Chen, Yanyun Liu, Perveen Shahnaz, Zhongnan Yang, Qingbo Yu, Hualing Mi
Summary: The RP8 gene is crucial for the processing of the rpoA transcript in Arabidopsis chloroplasts, affecting gene expression and chloroplast development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Kazusato Oikawa, Takuto Imai, Chonprakun Thagun, Kiminori Toyooka, Takeshi Yoshizumi, Kazuya Ishikawa, Yutaka Kodama, Keiji Numata
Summary: The researchers investigated the rate and trajectory of mitochondria in Arabidopsis thaliana mesophyll cells using confocal laser-scanning microscopy. They discovered that mitochondria migrate through wiggling during their association with chloroplasts, shedding light on the impact of mitochondria-chloroplast interaction on mitochondrial movement.
COMMUNICATIONS BIOLOGY
(2021)
Article
Plant Sciences
Daniel A. Careno, Soledad Perez Santangelo, Richard C. Macknight, Marcelo J. Yanovsky
Summary: Circadian rhythms are regulated by a set of clock genes, and this study identified LSM1 and XRN4 as factors involved in mRNA decay pathway that affects clock function in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Editorial Material
Cell Biology
Hui Zhang, Qihua Ling
Summary: This article introduces a significant cellular process, autophagy, which degrades unwanted or damaged macromolecules and organelles through the lysosome or vacuole for recycling. Selective autophagy was found to play a crucial role in regulating chloroplast protein import and enhancing stress tolerance in plants.
Article
Plant Sciences
Thomas Waksman, Noriyuki Suetsugu, Pawel Hermanowicz, James Ronald, Stuart Sullivan, Justyna Labuz, John M. Christie
Summary: Directional movements in plants are regulated by the protein RPT2, which is phosphorylated by phot1 and phot2. This phosphorylation is important for leaf positioning, phototropism, and chloroplast accumulation movement, and highlights the significance of the C-terminal region of NRL proteins in phot receptor signaling.
Article
Plant Sciences
Xin Chai, Xiushun Wang, Liwei Rong, Manfei Luo, Li Yuan, Qiuxin Li, Baoye He, Jingjing Jiang, Daili Ji, Min Ouyang, Qingtao Lu, Lixin Zhang, Jean-David Rochaix, Wei Chi
Summary: This study reveals a previously unknown function of the inner envelope membrane-bound AAA-ATPase FtsHi1 in maintaining R-loop homeostasis in chloroplasts of Arabidopsis thaliana. It is shown that FtsHi1 acts as an ATP-dependent helicase and prevents the accumulation of R-loops by unwinding both DNA-DNA and DNA-RNA duplexes. The dual function of FtsHi1 in protein import and chloroplast gene expression suggests a mechanical link between these two processes in higher plant chloroplasts.
Article
Biochemistry & Molecular Biology
Alexandre Magno Vicente, Nikolay Manavski, Paul Torben Rohn, Lisa-Marie Schmid, Antoni Garcia-Molina, Dario Leister, Charlotte Seydel, Leo Bellin, Torsten Moeurohlmann, Gregor Ammann, Stefanie Kaiser, Joeurorg Meurer
Summary: The sessile lifestyle of plants requires them to respond quickly to environmental stressors that affect their photosynthesis, growth, and crop yield. In this study, we found that heat, cold, and high light triggered significant changes in the expression of 42 epitranscriptomic factors in Arabidopsis, which are involved in chloroplast-associated functions. These expression changes were reversible upon deacclimation, indicating the role of epitranscriptomic players in acclimation processes. Chloroplast dysfunctions, particularly those induced by oxidative stress, led to signals that remodel chloroplast-associated epitranscriptomic expression patterns. The most prevalent RNA modification, N6-methyladenosine (m6A), was upregulated during cold treatment, along with the upregulation of components of the primary nuclear m6A methyltransferase complex. This resulted in increased m6A mRNA marks in cells. The presence of FIP37, a core component of the writer complex, played a crucial role in regulating thylakoid structure, photosynthetic functions, and the accumulation of photosystem I in the cold. These findings indicate the multifaceted roles of the cellular m6A RNA methylome in coping with cold stress, primarily associated with chloroplast functions and stabilization of photosynthesis.
PLANT COMMUNICATIONS
(2023)
Article
Plant Sciences
Eszter Bayer-Csaszar, Anja Joerg, Barbara Haertel, Axel Brennicke, Mizuki Takenaka
Summary: In plant organelles, PPR proteins with specific domain extensions recognize C-to-U RNA-editing sites. However, MEF28, an exception, can edit adjacent mitochondrial sites nad2-89 and nad2-90. The DYW domain of MEF28 provides unique target flexibility for two continuous cytidines. The gating domain of MEF28 also plays a role in target-site recognition. This information is valuable for designing specific synthetic RNA-editing tools.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Xinyu Lu, Zitong Yang, Wen Song, Jinlu Miao, Hanqing Zhao, Peiyun Ji, Tianli Li, Jierui Si, Zhiyuan Yin, Maofeng Jing, Danyu Shen, Daolong Dou
Summary: In this study, a class of FYVE domain-containing proteins was systematically investigated, and one secreted protein, PsFYVE1, was found to promote Phytophthora infection in both tobacco and soybean. It was also discovered that PsFYVE1 and NbRZ-1A co-regulated pre-mRNA alternative splicing and transcription levels of plant immunity-related genes, promoting disease development.
Article
Plant Sciences
Maria N. Danilova, Natalia Kudryakova, Anastasia S. Doroshenko, Amina G. Daminova, Ralf Oelmuller, Victor V. Kusnetsov
Summary: The study investigates the role of cytokinin in the senescence process of detached Arabidopsis leaves. It finds that cytokinin inhibits photosynthesis activity and chlorophyll content while promoting anthocyanin accumulation and ROS production. Additionally, cytokinin stimulates starch accumulation and alters the expression of extracellular cell wall enzyme genes.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Kwanuk Lee, Martin Lehmann, Melanie V. Paul, Liangsheng Wang, Manja Luckner, Gerhard Wanner, Peter Geigenberger, Dario Leister, Tatjana Kleine
Review
Biochemistry & Molecular Biology
Kwanuk Lee, Hunseung Kang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Su Jung Park, Hwa Jung Lee, Kwanuk Lee, Hunseung Kang
JOURNAL OF PLANT BIOLOGY
(2020)
Article
Plant Sciences
L. H. Tran, D. G. Lee, S. Jung
Summary: Our study reveals that specific wavelengths of different LEDs have differential effects on photosynthetic performance, photoprotection, and antioxidant properties, possibly by regulating the expression of phytochrome and cryptochrome genes in a light-quality-dependent manner.
Article
Plant Sciences
Ghazala Nawaz, Than Zaw Tun Sai, Kwanuk Lee, Su Jung Park, Sy Nguyen Dinh, Hunseung Kang
Summary: RNA helicases are cellular proteins that play a crucial role in regulating post-transcriptional mRNA metabolism in response to abiotic stress. This study investigated the functional role of a cabbage DEAD-box RH, BrRH37, in abiotic stress and ABA responses, and found that it may enhance plant growth and survival by its RNA chaperone activity.
JOURNAL OF PLANT BIOLOGY
(2021)
Article
Cell Biology
Kwanuk Lee, Dario Leister, Tatjana Kleine
Summary: Chloroplast-localized mTERF2 plays a crucial role in plant development, with its complete loss of function leading to embryo lethality and its reduction affecting plant development and chlorophyll content. Additionally, mTERF2 is required for the splicing of group IIB introns of ycf3 and rps12, highlighting its importance in regulating gene expression in chloroplasts.
Article
Plant Sciences
Jianzhong Hu, Jing Cai, Su Jung Park, Kwanuk Lee, Yuxia Li, Yao Chen, Jae-Young Yun, Tao Xu, Hunseung Kang
Summary: This study reveals that m(6)A methylation plays a crucial role in salt stress tolerance in Arabidopsis, with mutants of m(6)A writer components showing salt-sensitive phenotypes. It was demonstrated that VIR-mediated m(6)A methylation modulates reactive oxygen species homeostasis and mRNA stability of salt stress negative regulators. The findings highlight the important role of epitranscriptomic mRNA methylation in Arabidopsis salt stress response and its relationship with 3'UTR length and mRNA stability during stress adaptation.
Article
Entomology
Joon-Heum Park, Sunyo Jung
Summary: The expression of BjFeCh in transgenic rice helps alleviate NF-induced photobleaching by protecting the synthesis of chlorophyll intermediates and maintaining higher levels of porphyrin biosynthetic gene transcripts, contributing to understanding the resistance mechanism of NF-resistant crops and establishing a new weed control strategy.
JOURNAL OF PESTICIDE SCIENCE
(2021)
Article
Plant Sciences
Le Nguyen Tieu Ngoc, Su Jung Park, Jing Cai, Trinh Thi Huong, Kwanuk Lee, Hunseung Kang
Summary: Ribosomal RNA (rRNA) methylation is crucial for ribosome assembly and function, which are important for plant growth and stress responses. In this study, a new rRNA methyltransferase, RsmD, was identified in Arabidopsis chloroplasts, and it plays a role in plant adaptation to cold stress.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Agronomy
Sherzod Nigmatullayevich Rajametov, Kwanuk Lee, Hyo-Bong Jeong, Myeong-Cheoul Cho, Chun-Woo Nam, Eun-Young Yang
Summary: Tomatoes are often exposed to various environmental stresses, including cold stress, which can have negative effects on growth and development, leading to decreased yield and fruit quality. This study analyzed the physiological traits of 35 tomato accessions with different fruit types under control and low temperatures, showing significant reductions in plant height, leaf size, and fruit-related parameters under low temperature conditions. Multivariate analysis revealed positive correlations between different traits and classified the tomato accessions into four major groups based on their responses to low temperature stress. These results can be useful for breeding programs targeting cold-tolerant tomato cultivars.
Article
Agronomy
Sherzod Nigmatullayevich Rajametov, Kwanuk Lee, Hyo-Bong Jeong, Myeong-Cheoul Cho, Chun-Woo Nam, Eun-Young Yang
Summary: This research assessed the agronomical traits of 39 pepper accessions under different low temperature conditions, revealing significant impacts of low temperature stress on plant growth and productivity, as well as correlations with reproductive traits.
Review
Plant Sciences
Yeeun Kang, Kwanuk Lee, Ken Hoshikawa, Myeongyong Kang, Seonghoe Jang
Summary: The effects of climate change, such as increased global temperatures and abnormal weather events, are causing concerns for plant vegetation and crop productivity. This review focuses on the molecular processes of plants in response to heat stress, with emphasis on the classification and functions of heat shock proteins. Developing vegetable crops with enhanced thermotolerance using genetic tools is crucial to alleviate the adverse effects of heat stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Agronomy
Kwanuk Lee, Sherzod Nigmatullayevich Rajametov, Hyo-Bong Jeong, Myeong-Cheoul Cho, Oak-Jin Lee, Sang-Gyu Kim, Eun-Young Yang, Won-Byoung Chae
Summary: Climate change has an adverse impact on vegetable crops, particularly tomatoes, causing changes in physiological and biochemical responses which result in poor fruit quality and low yield. To develop heat-tolerant tomato cultivars, feasible and reliable screening strategies are needed, and the evaluation of various growth parameters can help determine the tomato's tolerance to high temperature.
Editorial Material
Plant Sciences
Kwanuk Lee, Hunseung Kang
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Soo-Young Chae, Kwanuk Lee, Jae-Wahng Do, Sun-Cheul Hong, Kang-Hyun Lee, Myeong-Cheoul Cho, Eun-Young Yang, Jae-Bok Yoon
Summary: In this study, QTL analysis was performed to identify genetic loci and alleles that play a crucial role in resistance to bacterial wilt (BW) in pepper plants. A genetic linkage map was constructed and a QTL on chromosome 01 was identified. These findings are valuable for developing SNP markers associated with BW resistance and breeding elite BW-resistant pepper cultivars.