期刊
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
卷 18, 期 9, 页码 2435-2442出版社
SPRINGER
DOI: 10.1007/s10008-014-2494-z
关键词
Dpa-melanin CNDs-graphene composites; Guanine and adenine; Simultaneous determination; Electrochemical biosensor
资金
- National Natural Science Foundation of China [21305119, 21375114, U1304214]
- Foundation of Henan Educational Committee [13A150768]
- State Key Laboratory of Chemo/Biosensing and Chemometrics [2012011]
Dopamine-melanin colloidal nanospheres (Dpa-melanin CNDs)-graphene composites-modified glassy carbon electrode (GCE) was prepared by a simple procedure and then successfully used to simultaneously determine guanine and adenine. Scanning electron microscopy (SEM) images and transmission electron microscopy (TEM) were used to characterize the morphology of the Dpa-melanin CNSs-graphene composite. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the electrode modifying process. Differential pulse voltammetry (DPV) was used to study the electrocatalytic activity toward the electrochemical oxidation of guanine and adenine. The modified electrode exhibited enhanced electrocatalytic behavior and good stability for the simultaneous determination of guanine and adenine compared with bare GCE. The electrochemical biosensor exhibited wide linear range of 0.5 to 150 mu M with detection limit of 0.05 and 0.03 mu M for guanine and adenine detection (S/N = 3), respectively. Furthermore, the biosensor showed high sensitivity, good selectivity, good reproducibility, and long-term stability to guanine and adenine detection. At the same time, the fabricated electrode was successfully applied for the determination of guanine and adenine in denatured DNA samples with satisfying results. These results demonstrated that Dpa-melanin CNSs-graphene composite was a promising substrate for the development of high-performance electrochemical biosensor.
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