期刊
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
卷 16, 期 6, 页码 2197-2201出版社
SPRINGER
DOI: 10.1007/s10008-012-1648-0
关键词
Biosensor; L-lactate; MoO3 nanowires; Direct electron transfer
资金
- Korean Ministry of Education, Science and Technology [NRF-2011-0031392, R31-2008-000-10029-0, S-2011-0292-000]
- Global Frontier Research Center for Advanced Soft Electronics
- National Research Foundation of Korea [2009-0094023] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Large-scale orthorhombic single-crystalline molybdenum trioxide nanowires were synthesized using a facile one-pot hydrothermal method. Lactate oxidase enzyme was immobilized on the nanowires to produce a highly sensitive electrochemical biosensor for l-lactate detection. At an applied potential of 0.5 V, the sensor exhibited a high sensitivity of 0.87 mu A/mM with a fast response to l-lactate (90% of response times within 10 s). A linear response was obtained over a concentration range from 0.5 to 8 mM with a detection limit of 0.15 mM (S/N = 3). The developed biosensor showed excellent reproducibility and operational stability, as well as the ability to be stored long term.
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