期刊
JOURNAL OF SHELLFISH RESEARCH
卷 32, 期 2, 页码 401-408出版社
NATL SHELLFISHERIES ASSOC
DOI: 10.2983/035.032.0220
关键词
oyster larvae; oyster juveniles; probiotic bacteria; Roseovarius oyster disease; shellfish hatchery; shellfish nursery; vibriosis; Vibrio tubiashii; Crassostrea virginica
资金
- Rhode Island Sea Grant
- Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [8 P20 GM103430-12]
- National Science Foundation under EPSCoR grant [0554548, EPS-1004057]
- Malaysian Government
- Universiti Putra Malaysia
- Office Of The Director
- Office of Integrative Activities [1004057] Funding Source: National Science Foundation
Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval and juvenile eastern oysters. The introduction of beneficial bacterial isolates in oyster hatcheries and nurseries for the biocontrol of bacterial diseases is a good alternative to the use of antibiotics. The goal of this study was to screen and characterize marine bacterial isolates as potential agents to prevent larval and juvenile mortality by the oyster pathogens Vibrio tubiashii and R. crassostreae. Screening of bacterial isolates from Rhode Island marine organisms and environment using agar-based assay methods for detection of antimicrobial activity against oyster pathogens led to the isolation of candidate probionts Phaeobacter sp. S4 and Bacillus pumilus RI06-95. Pretreatment of larval and juvenile oysters for 24 h with 10(2)-10(6) cfu/mL Phaeobacter sp. S4 or B. pumilus RI06-95 protected larval oysters against mortality resulting from challenge with R. crassostreae and V. tubiashii (relative percent survival (RPS) range, 9%-56%). These probiotics also protected juvenile oysters against challenge with V. tubiashii (RPS, 37%-50%). Probiotic isolates had no negative impact on oyster survival. Protection conferred to larvae against bacterial challenge was short-lived, lasting for only 24 h after removal of the probiotics from the incubation water. These results suggest the potential of marine bacterial isolates Phaeobacter sp. S4 and B. pumilus RI06-95 to serve as biocontrol agents to reduce the impact of bacterial pathogens in the culture of Crassostrea virginica.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据