期刊
JOURNAL OF SEPARATION SCIENCE
卷 37, 期 12, 页码 1438-1447出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201400129
关键词
Danhong injection; High-performance liquid chromatography; Liquid chromatography with mass spectrometry; Qualitative analysis; Quantitative characterization
资金
- Ministry of Science and Technology (MOST) in China [2010DFA32420]
- National Natural Sciences Foundation of China [90917003, 81130066, 81102766]
The present study aims to describe and exemplify an integrated strategy of the combination of qualitative and quantitative characterization of a multicomponent mixture for the quality control of traditional Chinese medicine injections with the example of Danhong injection (DHI). The standardized chemical profile of DHI has been established based on liquid chromatography with diode array detection. High-performance liquid chromatography coupled with time-of-flight mass spectrometry and high-performance liquid chromatography with electrospray multistage tandem ion-trap mass spectrometry have been developed to identify the major constituents in DHI. The structures of 26 compounds including nucleotides, phenolic acids, and flavonoid glycosides were identified or tentatively characterized. Meanwhile, the simultaneous determination of seven marker constituents, including uridine, adenosine, danshensu, protocatechuic aldehyde, p-coumaric acid, rosmarinic acid, and salvianolic acid B, in DHI was performed by multiwavelength detection based on high-performance liquid chromatography with diode array detection. The integrated qualitative and quantitative characterization strategy provided an effective and reliable pattern for the comprehensive and systematic characterization of the complex traditional Chinese medicine system.
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