期刊
JOURNAL OF SEPARATION SCIENCE
卷 35, 期 2, 页码 263-272出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201100780
关键词
HPLC-ESI-MSn; Hedyotis diffusa; Structural characterization
资金
- China Postdoctoral Science Foundation [20110491856]
The multiple bioactive constituents in Hedyotis diffusa Willd. (H. diffusa) were extracted and characterized by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn). The optimized separation condition was obtained using an Agilent ZorBax SB-C-18 column (4.6 x 150 mm, 5 mm) and gradient elution with water (containing 0.1% formic acid) and acetonitrile (containing 0.1% formic acid), under which baseline separation for the majority of compounds was achieved. Among the compounds detected, 14 iridoid glucosides, 10 flavonoids, 7 anthraquinones, 1 coumarin and 1 triterpene were unambiguously identified or tentatively characterized based on their retention times and mass spectra in comparison with the data from standards or references. The fragmentation behavior for different types of constituents was also investigated, which could contribute to the elucidation of these constituents in H. diffusa. The present study reveals that even more iridoid glycosides were found in H. diffusa than hitherto assumed. The occurrence of two iridoid glucosides and five flavonoids in particular has not yet been described. This paper marks the first report on the structural characterization of chemical compounds in H. diffusa by a developed HPLC-ESI-MSn method.
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