Article
Plant Sciences
Ghulam Khaliq, Sajid Ali, Shaghef Ejaz, Gholamreza Abdi, Yahya Faqir, Jiahua Ma, Mohammed Wasim Siddiqui, Asgar Ali
Summary: The effect of GABA treatment on papaya fruit stored at low temperatures was investigated. The treatment at 5 mM concentration inhibited chilling injury and enhanced antioxidant enzyme activities and defense system of the fruit.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jing Kang, Jun Gong, Lincheng Zhang, Zihan Gao, Qiaoli Xie, Zongli Hu, Guoping Chen
Summary: The novel tomato gene E6-2 plays a crucial role in regulating fruit ripening, with its expression being directly activated by MADS-RIN. Silencing of E6-2 leads to delayed ripening, reduced accumulation of carotenoids and lycopene, altered ethylene production, and changes in the content of various compounds. These findings highlight the importance of E6-2 in the control of tomato fruit ripening targeted by RIN.
Article
Plant Sciences
Mengjie Fu, Fenfen Li, Shengen Zhou, Pengyu Guo, Yanan Chen, Qiaoli Xie, Guoping Chen, Zongli Hu
Summary: The transcription factor SlGT31 positively regulates fruit ripening by binding to the promoters of ethylene biosynthesis genes ACO1 and ACS4. This study provides insight into the involvement of trihelix proteins in fruit ripening and transcriptional regulation, highlighting the role of SlGT31 as a positive modulator.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Yucheng Ming, Libo Jiang, Dongchao Ji
Summary: This review discusses the recent advances in epigenetic regulation of tomato fruit ripening, including DNA methylation, N6-Methyladenosine mRNA modification, histone demethylation/deacetylation, and non-coding RNA. This research contributes to a better understanding of the regulatory mechanisms governing tomato fruit ripening and provides new insights for precise modulation of these mechanisms.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jun-E Guo
Summary: Fruit development and ripening is a complex process regulated by plant hormones, transcription factors, and epigenetic modifications. This study focuses on the role of histone deacetylation gene SlHDT1 in tomato fruit ripening and found that it acts as a negative regulator, influencing ethylene and carotenoid biosynthesis.
Article
Plant Sciences
Feng Pan, Qianrong Zhang, Haisheng Zhu, Junming Li, Qingfang Wen
Summary: Insights into flavor formation during fruit ripening were obtained through metabolomic and transcriptomic analyses of cherry tomatoes. The study identified differentially accumulated metabolites related to flavor and observed changes in gene expression levels during ripening. The findings suggest the importance of metabolic and biosynthetic pathways in fruit ripening, and highlight the potential impact of genes related to flavor and firmness on metabolite accumulation.
Article
Biochemistry & Molecular Biology
Estelle Bineau, Jose Luis Rambla, Renaud Duboscq, Marie-Noelle Corre, Frederique Bitton, Raphael Lugan, Antonio Granell, Clemence Plissonneau, Mathilde Causse
Summary: This study aimed to investigate the transgressive behaviors of fruit metabolic content in tomatoes. Through the analysis of metabolome and transcriptome data, various modes of inheritance were identified and candidate genes that may drive changes in fruit volatile organic compound contents were proposed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yingwei Liu, Yangyang Gao, Moxian Chen, Yin Jin, Yongbin Qin, Gefei Hao
Summary: Fruit traits are important in determining plant fitness and quality. Gene regulatory networks in plants play a crucial role in determining fruit traits. However, there is a limited availability of specific databases for genes related to fruit traits. To address this limitation, we developed GIFTdb, a comprehensive database of fruit trait-related genes, and it provides various analysis tools to aid researchers in understanding gene functions and improving fruit traits.
Review
Agronomy
Mukesh Shivran, Nimisha Sharma, Anil Kumar Dubey, Sanjay Kumar Singh, Neha Sharma, Radha Mohan Sharma, Narendra Singh, Rakesh Singh
Summary: This article reviews the molecular interactions between rootstock and scion varieties in composite plants, as well as the impact of rootstocks on the production biology of economically important fruit crops. It also identifies key points to consider in conducting rootstock scion interaction experiments.
Article
Pharmacology & Pharmacy
Sang Wan Ryu, Yeo Ok Kim, Han-Byul Kim, Seog Bae Oh, Jeong Il Choi, Myung Ha Yoon
Summary: The recently identified molecule P7C3 has been found to have antinociceptive effects in tissue injury pain evoked by formalin injection, with involvement of the GABA system. P7C3 may increase GAD activity and GABA concentration in the spinal cord, leading to an antinociceptive effect against pain evoked by formalin injection.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Plant Sciences
Agustina P. Fortuny, Rodrigo A. Bueno, Javier H. Pereira da Costa, Maria Ines Zanor, Gustavo R. Rodriguez
Summary: This study found that in tomato fruits, heterosis and reciprocal effects were more pronounced at the metabolite level, being more influenced by genotypes than agronomic traits. Hybrids showed higher phenotypic diversity, with metabolites mainly being amino acids displaying more reciprocal effects and heterosis.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Shiming Qi, Shijie Zhang, Md. Monirul Islam, Ahmed H. El-Sappah, Fei Zhang, Yan Liang
Summary: The Tomato spotted wilt virus (TSWV) is highly destructive to tomato cultivation globally, with only the natural resistance gene Sw-5 demonstrating high resistance. Over time, however, resistance induced by Sw-5 weakens due to the global spread of TSWV, necessitating the exploration of other resistance genes and novel resources for breeding TSWV-resistant tomato varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Marina Martin-Valmaseda, Sama Rahimi Devin, German Ortuno-Hernandez, Cristian Perez-Caselles, Sayyed Mohammad Ehsan Mahdavi, Geza Bujdoso, Juan Alfonso Salazar, Pedro Martinez-Gomez, Nuria Alburquerque
Summary: CRISPR is a technology that selectively modifies the DNA of living organisms, adapted from naturally occurring genome-editing systems found in bacteria. Researchers reviewed the methods of introducing CRISPR/Cas-mediated genome editing into fruit tree species, and its impacts on tree development, yield, fruit quality, and tolerance to stresses. The gene-editing technology could enable the development of improved fruit crops, targeting different genetic segments or introducing traits without altering others. However, limitations such as limited regeneration and transformation protocols, genotype dependence, and the segregation of transgenic parts in the CRISPR system hinder its potential for fruit trees. The latest news on legislation and regulations regarding CRISPR/Cas-modified plants is also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Ahmed Sabra, Mahmoud Ahmed Amer, Khadim Hussain, Adel Zakri, Ibrahim Mohammed Al-Shahwan, Mohammed Ali Al-Saleh
Summary: This study collected tomato samples from Riyadh Region, Saudi Arabia and found that 52.4% of the samples were infected with Tomato brown rugose fruit virus (ToBRFV). Symptom observations were conducted on different host plants through mechanical inoculation, and the presence of ToBRFV was confirmed using ELISA and RT-PCR. The ToBRFV isolates in Saudi Arabia showed high nucleotide identity with isolates from other countries. Furthermore, the severity of ToBRFV infection was evaluated in commonly grown tomato cultivars in Saudi Arabia.
Article
Plant Sciences
Lisanne Smulders, Victoria Ferrero, Eduardo de la Pena, Maria J. Pozo, Juan Antonio Diaz Pendon, Emilio Benitez, Alvaro Lopez-Garcia
Summary: This study evaluates the impact of plant traits on root-associated bacterial communities and finds that plant resistance traits and genetic relatedness play important roles in shaping the composition of these communities. Furthermore, there is a positive correlation between plant resistance traits and bacterial diversity. These results highlight the potential of breeding to affect plant microbiomes.
Article
Biochemistry & Molecular Biology
Yoshinori Utsumi, Maho Tanaka, Chikako Utsumi, Satoshi Takahashi, Akihiro Matsui, Atsushi Fukushima, Makoto Kobayashi, Ryosuke Sasaki, Akira Oikawa, Miyako Kusano, Kazuki Saito, Mikiko Kojima, Hitoshi Sakakibara, Punchapat Sojikul, Jarunya Narangajavana, Motoaki Seki
Summary: The integrative omics approach revealed crosstalk among phytohormones during tuberous root development in cassava, with the effects of different hormones on various stages of plant growth being clarified.
PLANT MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tsubasa Shoji, Naoyuki Umemoto, Kazuki Saito
Summary: Plant domestication and improvement have led to mutational changes in transcriptional regulators of defense metabolism. Specialized compounds like alkaloids and terpenoids play essential roles in plant defense against herbivores and microbes. Transcription factors coordinately regulate defense metabolism genes by recognizing cis-regulatory elements in the promoter regions of target genes.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Atsushi Fukushima, Mikiko Takahashi, Hideki Nagasaki, Yusuke Aono, Makoto Kobayashi, Miyako Kusano, Kazuki Saito, Norio Kobayashi, Masanori Arita
Summary: The advancement of metabolomics has enabled rapid detection and quantification of cellular metabolites. However, shortcomings in data sharing still exist. To address these issues, RIKEN developed the RIKEN PMM, a database that stores plant metabolomic data and experimental metadata.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Tsubasa Shoji, Koki Moriyama, Nicolas Sierro, Sonia Ouadi, Nikolai Ivanov, Takashi Hashimoto, Kazuki Saito
Summary: In this study, the researchers investigated the regulation of nicotine biosynthesis in tobacco and identified several low-nicotine tobacco varieties lacking specific transcription factors. They characterized the newly identified alleles and examined their influence on alkaloid contents and gene expression related to nicotine biosynthesis. They also demonstrated the effect of a distal genomic region deletion on the expression of a key transcription factor and the alkaloid phenotype. These findings provide new insights into the regulation of nicotine biosynthesis and offer genetic resources for breeding low-nicotine tobacco.
Article
Plant Sciences
Kanade Tatsumi, Takuji Ichino, Natsumi Isaka, Akifumi Sugiyama, Eiko Moriyoshi, Yozo Okazaki, Yasuhiro Higashi, Masataka Kajikawa, Yoshinori Tsuji, Hideya Fukuzawa, Kiminori Toyooka, Mayuko Sato, Ikuyo Ichi, Koichiro Shimomura, Hiroyuki Ohta, Kazuki Saito, Kazufumi Yazaki
Summary: Plants produce a variety of lipophilic metabolites, some of which are secreted by cells. Triacylglycerol acts as a matrix lipid to facilitate the secretion of these lipophilic compounds.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Khurram Bashir, Daisuke Todaka, Sultana Rasheed, Akihiro Matsui, Zarnab Ahmad, Kaori Sako, Yoshinori Utsumi, Anh Thu Vu, Maho Tanaka, Satoshi Takahashi, Junko Ishida, Yuuri Tsuboi, Shunsuke Watanabe, Yuri Kanno, Eigo Ando, Kwang-Chul Shin, Makoto Seito, Hinata Motegi, Muneo Sato, Rui Li, Saya Kikuchi, Miki Fujita, Miyako Kusano, Makoto Kobayashi, Yoshiki Habu, Atsushi J. Nagano, Kanako Kawaura, Jun Kikuchi, Kazuki Saito, Masami Yokota Hirai, Mitsunori Seo, Kazuo Shinozaki, Toshinori Kinoshita, Motoaki Seki
Summary: This study demonstrates that exogenous application of ethanol can significantly enhance drought tolerance in Arabidopsis thaliana, rice, and wheat. Ethanol treatment leads to the upregulation of genes related to sugar and phenylpropanoid metabolism, as well as the accumulation of sugars and drought-tolerance-related amino acids. Ethanol treatment induces stomatal closure and decreases transpiration rate, resulting in enhanced drought tolerance. ABA signaling and acetic acid biosynthesis are involved in ethanol-mediated drought tolerance.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jichen Wang, Hideyuki Suzuki, Nanako Nakashima, Mariko Kitajima, Hiromitsu Takayama, Kazuki Saito, Mami Yamazaki, Naoko Yoshimoto
Summary: TvMAS1, an S-oxygenase, plays a crucial role in marasmin biosynthesis by catalyzing the oxygenation of S-(methylthiomethyl)-L-cysteine to produce marasmin in Tulbaghia violacea.
PLANT BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Amit Rai, Hideki Hirakawa, Megha Rai, Yohei Shimizu, Kenta Shirasawa, Shinji Kikuchi, Hikaru Seki, Mami Yamazaki, Atsushi Toyoda, Sachiko Isobe, Toshiya Muranaka, Kazuki Saito
Summary: In this study, a chromosome-scale genome assembly for Chinese licorice using HiFi sequencing reads was achieved, resulting in a high-quality plant genome assembly. The analysis revealed a recent whole-genome duplication event in licorice which contributed to its medicinal properties. The metabolic gene cluster analysis identified several gene clusters involved in the biosynthesis of glycyrrhizin.
Article
Biotechnology & Applied Microbiology
Tsubasa Shoji, Kazuki Saito
Summary: A group of specialized metabolites called steroidal glycoalkaloids (SGAs) are produced in Solanum species and their biosynthesis is regulated by the transcription factor JASMONATA-RESPONSIVE ETHYLENE RESPONSE FACTOR 4 (JRE4). In this study, the downregulation of a cluster of genes encoding nitrate transporter 1/peptide transporter family (NPF) members was observed in a low-SGA tomato mutant with a loss-of-function mutation in JRE4. The NPF transporters, which belong to a large family of plant membrane transporters, are induced by the defense-related phytohormone jasmonate and are potentially involved in the SGA pathway.
PLANT BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tsubasa Shoji, Kazuki Saito
Summary: The transcription factor JRE4 co-regulates the expression of RMA-type ligase gene SlRMA1 in tomato to prevent the overaccumulation of metabolites.
PLANT BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Tsubasa Shoji, Kazuki Saito
Summary: This study provides insights into the molecular mechanism of SGA biosynthesis and its regulation in eggplant, offering a theoretical basis for further understanding of the anti-nutritional defense in eggplant plants.
Correction
Chemistry, Medicinal
Naoko Yoshimoto, Takashi Asano, Ayuna Kisanuki, Chihiro Kanno, Machiko Asanuma, Mami Yamazaki, Isao Fujii, Kazuki Saito
JOURNAL OF NATURAL MEDICINES
(2023)
Article
Plant Sciences
Soichiro Honda, Yumiko Yamazaki, Takumi Mukada, Weiguo Cheng, Masaru Chuba, Yozo Okazaki, Kazuki Saito, Akira Oikawa, Hayato Maruyama, Jun Wasaki, Tadao Wagatsuma, Keitaro Tawaraya
Summary: Plants have evolved mechanisms to tolerate low P, including changing their membrane lipid composition. This study investigated the membrane lipid remodeling among different rice cultivars under P deficiency. The levels of phospholipids were lower in -P plants compared to +P plants, while the levels of non-phospholipids were higher in -P plants. Decomposition of phospholipids in roots correlated with low P tolerance. These findings suggest that rice cultivars remodel membrane lipids under P deficiency, contributing to their low P tolerance.
Review
Plant Sciences
Tsubasa Shoji, Takashi Hashimoto, Kazuki Saito
Summary: Tobacco is a widely cultivated crop, but its highly addictive nature poses a significant risk to human health. Recent research in molecular and genomics has led to advancements in understanding the metabolic and regulatory genes involved in nicotine biosynthesis, allowing the development of tobacco plants with ultra-low nicotine levels through mutational breeding, genetic engineering, and genome editing.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Tsubasa Shoji, Satoko Sugawara, Tetsuya Mori, Makoto Kobayashi, Miyako Kusano, Kazuki Saito
Summary: This study investigates the regulatory function of the transcription factor PhERF1 in petunia, which controls the synthesis of bioactive alkaloids and terpenoids. The study demonstrates that PhERF1 can induce the production of specialized steroids by transcriptional reprogramming. This highlights the potential of transcriptional regulators in producing specialized products.