Article
Agricultural Engineering
Rajesh Kona, Pavankumar Pallerla, Ramunaidu Addipilli, Prabhakar Sripadi, S. Venkata Mohan
Summary: The study evaluated the biosynthesis of lutein and beta-carotene by Scenedesmus sp. SVMIICT1 under different light intensities, finding that high lutein content was observed at 50 and 500 mu E/m(2)/s conditions. The study also showed that light intensities altered photosystem II and photosystem I in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Honey Mittal, Manika Khanuja
Summary: A novel MoSe2-PPy nanocomposite has been developed as a round the clock photocatalyst, showing enhanced degradation performance for dyes with increased photon absorption, improved charge separation, high surface area, optimized surface charge, conductive behavior, and slow charge transfer resistance.LC-MS studies showed the potential efficiency, reusability, and stability of this catalyst for dye degradation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Pauline Hiort, Christoph N. Schlaffner, Judith A. Steen, Bernhard Y. Renard, Hanno Steen
Summary: In liquid-chromatography-tandem-mass-spectrometry-based proteomics, we have developed a computational tool called multiFLEX-LF, which can quickly detect protein modifications and quantify their extent. It also allows for hierarchical clustering analysis to study modification dynamics and co-regulated modifications. This tool is capable of analyzing large data sets and driving the investigation of modification dynamics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Lichuang Cao, Zhenyu Wang, Dequan Zhang, Xin Li, Chengli Hou, Chi Ren
Summary: The study reveals that the phosphorylation status of myosin regulatory light chain plays a crucial role in regulating actomyosin dissociation, by reducing kinetic and total energy, stabilizing structure, and facilitating interaction between myosin and actin.
Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Agronomy
Archis Pandya, Bjorn Thiele, Andres Zurita-Silva, Bjorn Usadel, Fabio Fiorani
Summary: The study evaluated saponin content and their corresponding sapogenins in 114 different quinoa accessions, showing considerable variability in saponin content in quinoa. Approximately 75% of the genotypes were classified as low-saponin content lines, which is promising for current and future quinoa breeding programs. Additionally, twelve saponins of the oleanane type were identified, including a previously unreported compound, contributing to the valorization of genetic resources for quinoa.
Article
Chemistry, Analytical
Yuzhen Wang, Xuben Yu, Chuang Chen, Xiaoshan Zhang, Zhongjiang Ye, Jianhui Yang, Yaojie Chen, Zheng Xiang, Guanyang Lin, Ziye Zhou
Summary: The study aimed to establish and validate a method for determining the concentration of colistin in plasma and kidney homogenates using UPLC-MS/MS. The pharmacokinetic parameters of colistin in rats and the relationship between renal accumulation and time of administration were estimated. The method showed good linearity and met the FDA acceptance criteria for bioanalytical method validation. The established method provided a convenient and reliable way for colistin determination and was used for pharmacokinetic analysis and exploring the relationship between renal accumulation and dosing duration.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Review
Biochemical Research Methods
Jonas Carneiro Cruz, Israel Donizeti de Souza, Fernando Mauro Lancas, Maria Eugenia Costa Queiroz
Summary: Miniaturized liquid chromatography (LC) is recognized as an important analytical method in various research fields, offering higher resolution and lower consumption. However, limitations such as tubing clogging and small sample volumes hinder high throughput and sensitive analyses. Online coupling of sample preparation techniques can overcome these limitations and improve analytical efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Agriculture, Multidisciplinary
Sebastian Baur, Nicole Belle, Hans Hausladen, Sebastian Wurzer, Laura Brehm, Timo D. Stark, Ralph Huecklhoven, Thomas Hofmann, Corinna Dawid
Summary: This study quantitatively investigates the changes of SGAs and saponins in potatoes induced by post-harvest LED light exposure, using advanced analytical techniques. The results show that the SGA contents in potatoes exceed the safety limit, and the levels of saponins vary significantly among different cultivars. Moreover, cultivar-specific regulations of SGAs and saponins are observed due to light exposure.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Saikat Kumar Kuila, Sujit Kumar Guchhait, Debabrata Mandal, Partha Kumbhakar, Amreesh Chandra, Chandra Sekhar Tiwary, Tarun Kumar Kundu
Summary: The unique interfacial properties of 2D materials make them more functional than bulk materials in catalytic applications. In this study, 2D graphitic carbon nitride nanosheets were applied to cotton fabrics and nickel foam electrodes for self-cleaning and oxygen evolution reactions. The 2D-g-C3N4 coated interfaces showed higher surface roughness and enhanced hydrophilicity compared to bulk materials. The 2D-g-C3N4 demonstrated higher self-cleaning efficiency and lower overpotential for OER.
Article
Chemistry, Multidisciplinary
Jun Lin, Mengyu Xie, Dan Liu, Zhen Gao, Xiaoyan Zhao, Hongxia Ma, Sheng Ding, Shu mei Li, Song Li, Yanling Liu, Fang Zhou, Hao Hu, Tao Chen, He Chen, Min Xie, Bo Yang, Jun Cheng, Mingjun Ma, Yanyang Nan, Dianwen Ju
Summary: An aberrant peak near the light chain (LC) was observed during the development of a bispecific antibody (BsAb) product, and further analysis revealed that it was caused by a sequence variant resulting from aberrant splicing at the transcription level.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Linlin Dong, Nicole Bebrin, Konstantin Piatkov, Kojo Abdul-Hadi, Shinji Iwasaki, Mark G. Qian, Dong Wei
Summary: In the field of immuno-oncology, a highly sensitive and specific bioanalytical method is required for quantifying surrogate mouse antibodies, and the immunoaffinity enrichment based LC/MS/MS assay may provide an effective solution by differentiating analyte response from high endogenous immunoglobulin background.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hongjiang Xu, Yanan Li, Jing Zhang, Jinhua Zhang, Jianguang Lu, Xiquan Zhang, Ling Yang, Wenjie Zhao, Jun Feng
Summary: A rapid UHPLC-MS/MS assay method was developed for the determination of CMS E2 and colistin E2 in human plasma. The method demonstrated speed and high accuracy, and was successfully applied to the analysis of plasma samples obtained from Chinese healthy volunteers.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Yan Wu, Gillian Z. Miller, Jeff Gearhart, Graham Peaslee, Marta Venier
Summary: In 2018, popular children car seats in the United States were tested for fluorine content and PFAS. Results showed that PFAS were detected in 97% of the samples with total concentrations up to 268 ng/g, and fabric samples generally had higher levels than foam and laminated composites. Furthermore, the presence of side-chain fluorotelomer-based polymers and fluorotelomer (meth)acrylates indicated the potential degradation into PFAAs under UV light, highlighting a possible dermal route of exposure.
ENVIRONMENTAL POLLUTION
(2021)
Review
Obstetrics & Gynecology
Penelope D. Manta-Vogli, Kleopatra H. Schulpis, Yannis Dotsikas, Yannis L. Loukas
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2020)
Review
Nutrition & Dietetics
Penelope D. Manta-Vogli, Kleopatra H. Schulpis, Yannis Dotsikas, Yannis L. Loukas
CLINICAL NUTRITION
(2020)
Article
Pediatrics
Penelope D. Manta-Vogli, Kleopatra H. Schulpis, Yannis L. Loukas, Yannis Dotsikas
PEDIATRICS AND NEONATOLOGY
(2020)
Article
Medicine, Research & Experimental
Penelope D. Manta-Vogli, Kleopatra H. Schulpis, Yannis L. Loukas, Yannis Dotsikas
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
(2020)
Article
Chemistry, Applied
Amalia D. Kalampaliki, Steve Vincent, Suman Mallick, Hoang-Ngoan Le, Guillaume Barnoin, Yogesh W. More, Alain Burger, Yannis Dotsikas, Evagelos Gikas, Benoit Y. Michel, Ioannis K. Kostakis
Summary: This study presents a fluorogenic derivatization reagent with exclusive selectivity for primary amines, exhibiting heightened fluorescence and color change advantages, with insights from chemical quantum calculations and proof of concept from an LC analytical method for analyzing primary amines in a complex matrix.
Article
Endocrinology & Metabolism
Penelope D. Manta-Vogli, Kleopatra H. Schulpis, Yannis L. Loukas, Yannis Dotsikas
Summary: This study aimed to demonstrate MCA and LCA concentrations in Dried Blood Spots (DBS) of full-term breastfed infants, in relation to their birth weight. Results showed significantly lower MCA and LCA blood concentrations in low birth weight infants, and differences in acylcarnitine levels in infants with birth weight over 3,500g.
JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM
(2021)
Article
Chemistry, Analytical
Antonios-Dionysios G. Neofotistos, Kostas Gkountanas, Haris Boutsikaris, Yannis Dotsikas
Summary: A RP-HPLC method was developed and validated for the simultaneous determination of BC and BA in cough syrup, demonstrating satisfactory separation and clear evaluation of the target analytes. The method utilized a cyanopropyl column with a specific mobile phase and detection at 210 nm.
Article
Biochemistry & Molecular Biology
Nikolaos Kritikos, Aikaterini Iliou, Amalia D. Kalampaliki, Evangelos Gikas, Ioannis K. Kostakis, Benoit Y. Michel, Yannis Dotsikas
Summary: In this study, a novel xanthone analogue was used for the first time to derivatize the colorless analyte pregabalin, enabling the reduction of analysis time and achieving high yield and accuracy through optimized reaction conditions.
Article
Clinical Neurology
Theodore P. Parthimos, Kleopatra H. Schulpis, Alexandra D. Karousi, Yannis L. Loukas, Yannis Dotsikas
Summary: Elevated blood concentrations of neurotransmission-related amino acids are associated with improved neuropsychological performance after high-intensity exercise.
APPLIED NEUROPSYCHOLOGY-ADULT
(2022)
Article
Chemistry, Analytical
Konstantinos Kallinteris, Kostas Gkountanas, Ioannis Karamitros, Haris Boutsikaris, Yannis Dotsikas
Summary: A simple, precise, and cost-effective RP-IP-HPLC method was developed and validated for the determination of Ephedrine Hydrochloride in a nasal ointment. The method showed short run time, high recovery rates, and good precision.
Article
Chemistry, Medicinal
Kostas Gkountanas, Andelija Malenovic, Yannis Dotsikas
Summary: A novel chaotropic chromatography method for the quantitative determination of bupropion and its impurities is presented, following analytical quality-by-design (AQbD) principles. The method parameters were selected using experimental design and Monte Carlo simulations. The method was validated according to ICH guidelines and applied to the analysis of tablets containing bupropion hydrochloride.
Article
Chemistry, Physical
Anna Apostolidi, Chrystalla Protopapa, Angeliki Siamidi, Marilena Vlachou, Yannis Dotsikas
Summary: This study describes the development and validation of a novel RP-HPLC method for simultaneous quantitation of bupropion hydrochloride (BUP·HCl) and naltrexone hydrochloride (NTX·HCl) during the dissolution process. The method was validated according to ICH guidelines and fulfilled the specifications for specificity, linearity, accuracy, precision, and stability. The robustness of the method was evaluated using experimental design and statistical approaches.
Article
Chemistry, Medicinal
Ivi Antoniadou, Maria Georgiou, Yannis Dotsikas, Alexandra Lamprou, Nikolaos Lougiakis, Nicole Pouli, Nikolaos Karousis, Yannis Loukas, Ioulia Tseti, Panagiotis Marakos, Andreas Papapetropoulos
Summary: Hydrogen sulfide (H2S) is a gasotransmitter that reduces inflammation and itching. Bifunctional molecules with antihistamine and H2S-releasing pharmacophores were synthesized and tested, showing dose-dependent H2S release and histamine blocking activity. The most potent compounds demonstrated superior antipruritic efficacy and reduced sedative effects, indicating the potential for targeted and limited side effects.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Nikolaos Kritikos, Anna Bletsou, Christina Konstantinou, Antonios-Dionysios Neofotistos, Constantinos Kousoulos, Yannis Dotsikas
Summary: Migration studies in pharmaceutical analysis use wide-scope screening methods to detect contaminants resulting from formulation and material interactions. Liquid Chromatography or Gas Chromatography coupled to mass spectrometry (LC-MS and GC-MS) are used for both qualitative and quantitative data analysis. However, mass spectrometry may not universally detect all analytes, and the application of uncertainty factors to limit potential analytes escaping report and evaluation is still debated. This study evaluates the response of species detected in migration studies and creates a strategy and evaluation mechanism for their classification.
Article
Chemistry, Medicinal
Chrysa Daousani, Vangelis Karalis, Yannis L. Loukas, Kleopatra H. Schulpis, Konstantinos Alexiou, Yannis Dotsikas
Summary: Dried blood spot (DBS) microsampling is widely used in newborn screening and neonatal studies. However, the varying neonatal hematocrit (Ht) values can cause analytical errors, and using fixed Ht for calibration is not representative of all neonates. A computational approach was developed to address the Ht effect in neonatal DBS assays, proposing correction factors to adjust the concentrations. The study highlights the importance of considering the Ht effect in neonatal studies and suggests further investigation into its correlation with other clinical variables.
