Article
Green & Sustainable Science & Technology
Matteo Perra, Francisco-Javier Leyva-Jimenez, Maria Letizia Manca, Maria Manconi, Hiba N. Rajha, Isabel Borras-Linares, Antonio Segura-Carretero, Jesus Lozano-Sanchez
Summary: The aim of this study was to optimize the recovery of phenolic compounds from Cannonau grape pomaces using Pressurized Liquid Extraction (PLE). Response Surface Methodology (RSM) based on a Central Composite Design 23 model was used to perform PLE experiments. The results showed that the optimum values for phenolics extraction were 130 degrees C, 55% ethanol, and 22 min. The proposed experimental model proved to be a valuable alternative method for the extraction of bioactive compounds from wine-making by-products.
JOURNAL OF CLEANER PRODUCTION
(2023)
Review
Chemistry, Analytical
Aurelia Magdalena Pisoschi, Florin Iordache, Loredana Stanca, Adriana Ionescu Petcu, Letitia Purdoiu, Ovidiu Ionut Geicu, Liviu Bilteanu, Andreea Iren Serban
Summary: Aflatoxins are highly toxic secondary metabolites of fungi that are classified as poisonous mycotoxins. They are mainly produced by Aspergillus flavus and Aspergillus parasiticus in cereals, peanuts, and animal feeds. Sample preparation techniques, chromatographic methods, and detection techniques are crucial in aflatoxin analysis. Selectivity in analysis is achieved using bio-recognition elements like enzymes, antibodies, or DNA sequences. Determination of aflatoxins is essential for food safety due to their high toxicity and presence in food and agricultural products. The choice of analysis method depends on the sample nature, available equipment, toxin concentration range, sensitivity, detection limit, precision, selectivity, required analysis time, and personnel training.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Murat Kaya, Mustafa Cam
Summary: Eritadenine, a cholesterol lowering component found in Lentinula edodes mushrooms, can be effectively extracted using pressurized liquid extraction (PLE) technique with water as the solvent at a temperature of 142 degrees C. PLE also provides higher yields of total phenolics and antioxidant capacity compared to classical aqueous ethanol extraction. Eritadenine is thermally stable, making it suitable for enriching thermally treated foods.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Chemistry, Applied
Hacer Sibel Karapinar, Ali Bilgic
Summary: A novel modified magnetic adsorbent was synthesized for efficient detection of aflatoxins in nuts, with optimized extraction parameters. The synthesized adsorbent showed excellent performance in extracting aflatoxins quickly and accurately.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Engineering, Chemical
Thalles Allan Andrade, Fabiane Hamerski, Damian Estuardo Lopez Fetzer, Maria Cinta Roda-Serrat, Marcos Lucio Corazza, Birgir Norddahl, Massimiliano Errico
Summary: This study aimed to investigate the optimum conditions for ultrasonic-assisted pressurized liquid extraction of anthocyanins from Aronia melanocarpa pomace. Through optimization of pressure, temperature, ultrasound intensity, and solvent concentration, the total anthocyanin content extracted was increased by 19% at 80 degrees Celsius.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Veterinary Sciences
Martha Maggira, Ioannis Sakaridis, Maria Ioannidou, Georgios Samouris
Summary: This study compared three available ELISA kits for the detection of aflatoxins in feed samples. All three kits showed good performance and were validated. Furthermore, the results of both ELISA and HPLC tests confirmed their suitability for mycotoxin analysis.
VETERINARY SCIENCES
(2022)
Article
Food Science & Technology
Mercedes Brenes-Alvarez, Eva Maria Ramirez, Manuel Brenes, Pedro Garcia-Garcia, Eduardo Medina, Concepcion Romero
Summary: The presence of acrylamide raises significant concerns among consumers. A new analytical method has been developed to quantify acrylamide in black ripe olives, which has shown excellent results in terms of accuracy and precision limits. The method reduces the analysis time and could be extended to other foods.
Article
Chemistry, Applied
Nishtha Khansili, Prayaga Murali Krishna
Summary: Cerium oxide nanoparticles (NanoCeO2) and bentonite (Bent) based nanocomposite (CeO2-Bent) aqueous sensor drop casted on paper substrate has been used to detect food borne Aflatoxins (AFs) by Curcumin (Cur) functionalized redox reaction mediated colorimetric detection. The sensor reagent further drop casted on paper showed a quick determination for presence of AFs. The calculated LOD indicated values for different food items.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Dymek, Jaroslaw Widelski, Krzysztof Kamil Wojtanowski, Paulina Ploszaj, Rostyslav Zhuravchak, Tomasz Mroczek
Summary: Alkaloids from the Lycopodiaceae family have potential antioxidant, antidepressant, and AChE inhibitory effects, which are crucial for treating neurodegenerative diseases. By optimizing extraction conditions using modern PLE, efficient extraction of alkaloids was achieved with high recovery rates, analyzed by HPLC/ESI-QTOF-MS for potential AChE inhibitors.
Article
Engineering, Environmental
Chenhuan Wang, Jie Li, Qianqian Wang, Qiuhua Wu, Xiaodong Shi
Summary: A new fluorine-functionalized covalent organic framework (F-COF) was designed and fabricated for the first time, showing enhanced adsorption capability for aflatoxins. By coupling F-COF with high-performance liquid chromatography, a rapid and sensitive method for determining aflatoxins was established.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Biophysics
Yiqian Kong, Zongyi Li, Lili Zhang, Juncheng Song, Qi Liu, Yinghua Zhu, Na Li, Lili Song, Xiangyang Li
Summary: This study developed a dual mode aptasensor and an intelligent sensor detection platform for rapid and accurate on-site detection of aflatoxin B1 in food. The sensor utilized special nanomaterials and a fluorescent probe to achieve fluorescence/colorimetric detection without the need for material and reagent replacement. The collected signals can be analyzed and modeled on a developed artificial neural network platform, enabling visual intelligent sensing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Marko Krstic, Nemanja Teslic, Perica Boskovic, Darija Obradovic, Zoran Zekovic, Anita Milic, Branimir Pavlic
Summary: This study aimed to extract bioactive compounds from the Ranco genotype of garlic using subcritical water extraction (SWE) and pressurized liquid extraction (PLE). Results showed that PLE provided significant advantages in terms of yield and antioxidant activity of garlic extracts, indicating that high-pressure processes can be used as clean and green procedures for the extraction of garlic bioactives.
Article
Food Science & Technology
Di Yuan, Liangxiao Zhang, Fei Ma, Peiwu Li
Summary: This study developed a rapid, accurate, and cost-effective method for the simultaneous quantification of aflatoxins and benzo(a)pyrene in lipid matrices using solid-phase extraction (SPE) combined with high-performance liquid chromatography and photochemical post-column reactor fluorescence spectroscopy (HPLC-PHRED-FLD). The method showed low limits of quantification and detection, high recovery rates, and was successfully applied to analyze oil samples for the occurrence of aflatoxins and benzo(a)pyrene.
Review
Chemistry, Applied
Daniel B. Alcantara, Ana P. Dionisio, Adriana G. Artur, Brenda K. S. Silveira, Amanda F. Lopes, Jhonyson A. C. Guedes, Licia R. Luz, Ronaldo F. Nascimento, Gisele S. Lopes, Helen H. M. Hermsdorff, Guilherme J. Zocolo
Summary: Selenium is crucial for mammals and human health, with Brazil nuts being highlighted as a rich source of bioavailable selenium. Researchers have investigated various analytical methods and discussed the chemical forms of selenium consumed through dietary intake, aiming to understand the importance of adequate selenium intake and determine effective methods for selenium species analysis.
Article
Chemistry, Applied
Mingming Xu, Zhijun Zhou, Lin Hao, Zhi Li, Jie Li, Qianqian Wang, Weihua Liu, Chun Wang, Zhi Wang, Qiuhua Wu
Summary: Three novel hyper-crosslinked polymers with large specific surface area and excellent chemical stability were fabricated using heterocyclic phenyl-imidazole as monomer. These polymers were used as solid phase extraction adsorbent for sensitive determination of aflatoxins in rice samples. The established method provided wide linear range, low detection limit and satisfactory recovery, and the adsorption mechanism was explored. This work provides a powerful tool for simultaneous determination of aflatoxins in food grains with great application potential in aflatoxin monitoring.
Article
Chemistry, Applied
Serena Rizzo, Rita Celano, Luca Campone, Luca Rastrelli, Anna Lisa Piccinelli
Summary: The application of salting out assisted liquid-liquid extraction for the rapid and simple simultaneous determination of pyrrolizidine alkaloids and related N-oxide in honey and pollen was evaluated. The results showed that the developed procedure had excellent extraction efficiency and recoveries for all target analytes, with low quantification limits. Furthermore, the method was successfully applied to a large number of honey and pollen samples, revealing a high contamination rate.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Stefania Pagliari, Ciro Cannavacciuolo, Rita Celano, Sonia Carabetta, Mariateresa Russo, Massimo Labra, Luca Campone
Summary: The valorisation of food by-products is important for improving the economic and environmental sustainability of the food production chain. This study focuses on the artichoke plant and aims to develop a novel strategy for utilizing its non-edible parts. By using green pressurised-liquid extraction and other analytical methods, bioactive-rich extracts with high antioxidant capacity were obtained.
Article
Biochemistry & Molecular Biology
Osmany Cuesta-Rubio, Ingrid Marquez Hernandez, Mercedes Campo Fernandez, Iran Rodriguez-Delgado, Rodny Montes De Oca Porto, Anna Lisa Piccinelli, Rita Celano, Luca Rastrelli
Summary: The chemical composition and antioxidant potential of Ecuadorian propolis were investigated. Six types of Ecuadorian propolis were defined based on their secondary metabolite composition, and 59 compounds were reported for the first time. The results showed that the variation in chemical composition affected the antioxidant potential of the propolis.
Article
Food Science & Technology
Alessandra De Giani, Stefania Pagliari, Jessica Zampolli, Matilde Forcella, Paola Fusi, Ilaria Bruni, Luca Campone, Patrizia Di Gennaro
Summary: Cinnamon polyphenols have antimicrobial activity against Staphylococcus aureus and Bacillus subtilis, but this activity is lost after in vitro digestion. However, they exhibit strong prebiotic potential for probiotic strains. Additionally, the secondary metabolites produced from cinnamon extract or its digested form have protective effects against colorectal tumor cells.
Review
Food Science & Technology
Ciro Cannavacciuolo, Stefania Pagliari, Jessica Frigerio, Chiara Maria Giustra, Massimo Labra, Luca Campone
Summary: Usual extraction processes for analyzing foods, supplements, and nutraceutical products involve massive amounts of organic solvents causing negative impact on the environment and human health. Natural deep eutectic solvents (NADES), a new class of green solvents, are sustainable, biodegradable, non-toxic and cost-effective, making them a valid alternative to conventional solvents. This review provides an overview of NADES, including their classification, preparation methods, physicochemical properties, toxicity, and their potential application as extractants of bioactive compounds in food analysis.
Article
Food Science & Technology
Stefania Pagliari, Matilde Forcella, Elena Lonati, Grazia Sacco, Francesco Romaniello, Pierangela Rovellini, Paola Fusi, Paola Palestini, Luca Campone, Massimo Labra, Alessandra Bulbarelli, Ilaria Bruni
Summary: Cinnamon bark is widely used for its organoleptic features in food and has been found to have beneficial effects on human health. Cinnamon extracts contain antioxidant, anti-inflammatory, and anticancer biomolecules. This study evaluated the antioxidant and anti-inflammatory bioactivity of digested cinnamon extract and found that the digestive process reduces the polyphenol content while maintaining other bioactive compounds and functional effects at the cellular level.
Article
Chemistry, Applied
Serena Rizzo, Rita Celano, Anna Lisa Piccinelli, Simona Serio, Mariateresa Russo, Luca Rastrelli
Summary: An analytical platform for the detection of pyrrolizidine alkaloids (PAs) in various products was proposed. The platform employed a wide-scope suspect screening method for characterization of PAs and a target screening method for high-throughput detection of 118 PAs. The platform showed high effectiveness in detecting and identifying both target and untarget PAs, as demonstrated by the screening of commercial samples.
Review
Biochemistry & Molecular Biology
Osmany Cuesta-Rubio, Lianet Monzote, Roberto Fernandez-Acosta, Gilberto Lazaro Pardo-Andreu, Luca Rastrelli
Summary: This article reviews the chemical and biological research progress of nemorosone, a natural compound with promising antiproliferative effects. Nemorosone has shown activity against various malignancies and has the potential as an anticancer and anti-parasitic drug. It exhibits cytotoxicity, induces apoptosis and ferroptosis, and also possesses antimicrobial capabilities.
Article
Nutrition & Dietetics
Giuseppe Castaldo, Luigi Schiavo, Imma Pagano, Paola Molettieri, Aurelio Conte, Gerardo Sarno, Vincenzo Pilone, Luca Rastrelli
Summary: Ketogenic nutrition-based dietary protocols, such as enteral protein (NEP) and enteral hypocaloric (NEI), are effective and safe treatments for weight reduction and improvement of glycemic and lipid profiles in obese patients before bariatric surgery (BS).
Article
Biochemistry & Molecular Biology
Ciro Cannavacciuolo, Stefania Pagliari, Chiara Maria Giustra, Sonia Carabetta, Werther Guidi Nissim, Mariateresa Russo, Paola Branduardi, Massimo Labra, Luca Campone
Summary: The comparative chemical composition of different parts of Faustrime fruits was analyzed using GC-MS and UHPLC-HRMS QTof. The data obtained were combined to define the fruit's complex antioxidant activity. The principal component analysis showed the occurrence of specific compounds in different parts of the fruit, such as alpha-bisabolol and alpha-trans-bergamotol in the pulp and albedo, hexanoic acid in the seeds, and various coumarins and phenolics in the peels. The higher radical scavenging activity of the pulp was attributed to the presence of citric acid.
Article
Food Science & Technology
Tommaso Gorini, Valerio Mezzasalma, Marta Deligia, Fabrizio De Mattia, Luca Campone, Massimo Labra, Jessica Frigerio
Summary: The molecular approach of DNA barcoding is widely used in many European countries for the characterization and traceability of food products. This study collected commonly defrauded food products and identified efficient workflows for species identification, with 21.2% of products found to be defrauded. DNA barcoding analysis correctly identified 88.2% of specimens, confirming its reliability in ensuring food quality and safety.
Article
Biodiversity Conservation
Irena Mincheva, Zheko Naychov, Cristo Radev, Ina Aneva, Luca Rastrelli, Maria Kamusheva, Nikolay Nikolov, Ekaterina Kozuharova
Summary: Rhodopes Mountain is poorly studied in terms of ethnobotany. Our preliminary observation suggests that the local population still relies on previously collected medicinal plants. In this study, we aimed to evaluate the contemporary usage of medicinal plants and the associated health conditions in the Central and East Rhodopes. Through in-depth field research and interviews, we found that 92 informants mentioned the use of 114 plant species. The study shows that medicinal plants are most frequently mentioned in relation to diseases affecting the nervous, respiratory, digestive, and cardiovascular systems.
Article
Biology
Catello Pane, Gelsomina Manganiello, Antonella Vitti, Rita Celano, Anna Lisa Piccinelli, Enrica De Falco
Summary: The cultivation of false yellowhead was studied and its phytochemical extracts were found to have potential in inhibiting weed growth and reducing spinach disease. Plant extracts obtained through sustainable methods can be effective in agricultural applications.
Article
Chemistry, Applied
Serena Rizzo, Rita Celano, Anna Lisa Piccinelli, Mariateresa Russo, Luca Rastrelli
Summary: An analytical procedure for the screening and determination of pyrrolizidine alkaloids (PAs) in food supplements, herbal infusions, honey, and teas was successfully validated. The study found that 10% of the analyzed samples were contaminated above the maximum levels (MLs) set by European legislation. Contamination of herbal infusions of mixed plants raised potential health concerns, with 23% showing PA levels above ML. Although a high number of PAs not included in the regulation were detected in honey and herbal food supplements, their contribution to overall levels in honey was not significant.
Article
Chemistry, Applied
Sofia Cavini, Lorenzo Guzzetti, Francesca Givoia, Maria Elena Regonesi, Patrizia Di Gennaro, Chiara Magoni, Luca Campone, Massimo Labra, Ilaria Bruni
Summary: This study aims to assess the yield and polymerization degree of inulin in artichoke by-products, and proposes optimal conditions for utilizing artichoke waste, providing possibilities for a circular economy process within the artichoke supply chain.
NATURAL PRODUCT RESEARCH
(2022)
