Article
Biochemical Research Methods
Florine Hallez, Audrey Combes, Charlotte Desoubries, Anne Bossee, Valerie Pichon
Summary: A new LC-MS/MS method was developed to detect alkylated peptides resulting from exposure to sulfur mustard. The method accurately identified alkylated sites on proteins and allowed for quantification with satisfying repeatability. It also showed sensitivity towards detecting alkylated peptides resulting from in vitro incubation, suggesting its potential for future analysis of blood samples exposed to sulfur mustard in vivo.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Manoj Bob Kusuma, Ravisekhar Kashibhatta, Sandeep S. Jagtap, Vijay Nadawade, Suresh Adsul, Sudheer Moorkoth, Krishnamurthy Bhat, Rustom Mody, Praveen Vithala
Summary: A sensitive method using LC-MS/MS was developed for the determination of PEG-IFN-alpha-2b in human serum, which showed bioequivalence in a clinical study. The method demonstrated high sensitivity, precision, and accuracy, making it a promising approach for estimating PEG-IFN-alpha-2b and potentially other pegylated molecules.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Ofir Schuster, Yafit Atiya-Nasagi, Osnat Rosen, Anat Zvi, Itai Glinert, Amir Ben Shmuel, Shay Weiss, Orly Laskar, Liron Feldberg
Summary: A combined approach of immuno-magnetic viral isolation and mass spectrometry analysis was developed for the identification of SARS-CoV-2. The method demonstrated high reliability, specificity, and sensitivity, and could potentially serve as a complementary assay to RT-qPCR. This methodology can also be applied to other viruses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jie Hong, Shuchen Xin, Rui Min, Yongqian Zhang, Yulin Deng
Summary: The study proposed a peptide-based strategy to diagnose type 2 diabetes by quantifying hemoglobin tryptic peptides, identifying sensitive and insensitive peptides to glucose, and validating three promising biomarkers for diabetes diagnosis.
Article
Chemistry, Multidisciplinary
Manasses Jora, Kayla Borland, Scott Abernathy, Ruoxia Zhao, Melissa Kelley, Stefanie Kellner, Balasubrahmanyam Addepalli, Patrick A. Limbach
Summary: LC-MS/MS is currently the leading technique for studying RNA and its modifications. The discovery of a non-natural ribonucleoside in tRNA hydrolysate highlights the importance of minimizing artifacts during sample preparation. Optimizing digestion conditions can lead to more accurate detection of new RNA modifications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Food Science & Technology
Xin Luo, Mingyan Ai, Yongyan Wu, Qia Wang, Hongbo Song, Qun Huang, Jiankang Lu
Summary: The study investigated the effects of malondialdehyde (MDA) oxidation treatment on the immunoreactivity and digestion of amandin. The results showed that MDA treatment significantly reduced the immunoreactivity of amandin and led to protein aggregation, resulting in slowed down digestion.
FOOD QUALITY AND SAFETY
(2023)
Article
Food Science & Technology
Xin Luo, Mingyan Ai, Yongyan Wu, Qia Wang, Hongbo Song, Qun Huang, Jiankang Lu
Summary: MDA treatment reduces the immunoreactivity of amandin, leads to protein aggregation, slows down the digestion of amandin, and alters the immunoreactivity of amandin during digestion.
FOOD QUALITY AND SAFETY
(2023)
Article
Pharmacology & Pharmacy
Takashi Mochizuki, Kaito Shibata, Takafumi Naito, Kumiko Shimoyama, Noriyoshi Ogawa, Masato Maekawa, Junichi Kawakami
Summary: A validated LC-MS/MS method was developed for monitoring serum tocilizumab concentrations in rheumatoid arthritis patients. The method involved rapid digestion of serum samples using immobilized trypsin without the need for immunoglobulin G purification. The results showed good accuracy and precision of the method, and a positive correlation between serum tocilizumab concentrations measured using this method and the enzyme-linked immunosorbent assay.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Lili Jin, Haibo Ding, Volkan Degirmenci, Hongchuan Xin, Qifan Miao, Qiuyu Wang, Dianbao Zhang
Summary: AWRK6, a synthesized peptide based on the natural peptide dybowskin-2CDYa, was analyzed in rat plasma using a LC-MS/MS method. Pharmacokinetic studies showed AWRK6 peaked in rat plasma at 1.2 hours after intraperitoneal injection and had a terminal half-life of approximately 2.8 hours.
Article
Biochemistry & Molecular Biology
Lingling Liu, Jianwei Chen, Xiang Li
Summary: The research aimed to investigate the alpha-glucosidase inhibitory activity of peptides derived from Changii Radix protein under various enzymatic and digestive conditions. The most active fraction, GID-3-SG2-RP-2, exhibited the highest effect, suggesting the potential role of Changii Radix peptides in the prevention and management of type 2 diabetes.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Mingyue Zhang, Yingying Li, Yingying Zhang, Chaodi Kang, Wentao Zhao, Nan Ren, Wenping Guo, Shouwei Wang
Summary: This study introduced a rapid sample preparation method, established a rapid LC-MS/MS method for meat species identification, successfully screened 35 species-specific peptides, detected the meat species of 20 processed meat products, and found potentially adulterated samples.
Article
Food Science & Technology
Xiaoxu Luan, Meiqin Feng, Jian Sun
Summary: The study explored the in vitro antioxidant activity of Lactobacillus plantarum CD101 and its impact on antioxidant peptide formation in fermented sausage. L. plantarum CD101 mainly distributed antioxidant substances on the cell surface and extracellular secretions. The potential benefit of L. plantarum CD101 in inhibiting oxidative rancidity and promoting protein degradation was observed, with a weak effect on promoting antioxidant peptides.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Yangfan Leng, Liubin Yang, Hangxin Zhu, Dongqin Li, Siyi Pan, Fang Yuan
Summary: In this study, blueberry extracellular vesicles (B-EVs) were collected and purified, and their stability during food storage and processing conditions was evaluated. The results showed that B-EVs had desirable properties and could affect human intestinal function.
Article
Chemistry, Applied
Jiangbing Qiu, Lei Zhang, Yadong Cao, Peng Zhao, Jingrui Zhang, Aifeng Li
Summary: This study assessed the bioaccessibility of PSTs in cooked shellfish using an in vitro human digestion model. The results showed that different cooking methods reduced the content of PSTs in shellfish, but did not significantly impact their bioaccessibility. Transformation or degradation of PSTs occurred during the simulated digestion process.
Article
Chemistry, Medicinal
Yodying Yingchutrakul, Sucheewin Krobthong, Kiattawee Choowongkomon, Phakorn Papan, Pawitrabhorn Samutrtai, Thanisorn Mahatnirunkul, Thitikorn Chomtong, Nitipol Srimongkolpithak, Theeranuch Jaroenchuensiri, Chanat Aonbangkhen
Summary: This study identified and investigated the effects of a novel multifunctional peptide from Lingzhi on melanogenic enzymes in melanoma cells through a targeted-proteomics approach. The synthesized octapeptide exhibited strong antioxidant activity and regulated the expression of specific proteins involved in pigmentation pathways. These findings contribute to the understanding of the molecular functions of the multifunctional octapeptide on melanogenic enzymes and support its potential as a therapeutic and cosmetic ingredient.
Article
Biochemical Research Methods
Trine Gronhaug Halvorsen, Maren Christin Stillesby Levernaes, Cecilie Rosting
Summary: This study evaluated the use of vacuum matrix-assisted ionization mass spectrometry for identification and determination of tryptic peptides from ProGRP protein. The vacuum ionization conditions lowered the detection limits and enabled detection of more digested protein peptides. Coupling with high resolution MS, vMAI showed advantages in determining MS/MS fragmentation conditions and differentiation of intact protein isoforms.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Eleonora Pizzi, Trine Gronhaug Halvorsen, Christian J. Koehler, Leon Reubsaet
Summary: This study describes the immobilization of trypsin on cellulose paper using different methods, enabling a fundamental understanding of protein binding and enzyme activity on paper. Functionalized discs outperform untreated discs in protein binding, with potential demonstrated in digesting complex biological samples.
Article
Immunology
Erik Waage Nielsen, Yoav Miller, Ole-Lars Brekke, Joost Grond, Anh Hoang Duong, Hilde Fure, Judith Krey Ludviksen, Kristin Pettersen, Leon Reubsaet, Rigmor Solberg, Harald Thidemann Johansen, Tom Eirik Mollnes
Summary: This study established a new porcine model to evaluate systemic ischemia-reperfusion injury. The results showed that treatment with C1-INH had little effect on the changes induced by IRI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Minh Thao Nguyen, Trine Gronhaug Halvorsen, Bernd Thiede, Leon Reubsaet
Summary: This study investigates the digestion of proteins by trypsin covalently bound to paper compared to trypsin in solution. The results show that trypsin on paper retains approximately 50% of its activity and exhibits greater stability than trypsin in solution. In-solution digestions also yield more protein identifications and higher coverage compared to on-paper digestions.
SEPARATION SCIENCE PLUS
(2022)
Article
Biochemical Research Methods
Christina Johannsen, Anam Ul Haq, Leon Reubsaet, Trine Gronhaug Halvorsen
Summary: The study introduces a novel on-paper device combining sample collection with affinity sample pretreatment. The functionality of the device has been demonstrated in the laboratory setting, showing potential for detecting low abundant biomarkers.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: Selective and efficient sample clean-up is important in mass spectrometric protein and proteomics analyses. This review discusses the utility of molecularly imprinted polymers (MIPs) for protein capture from biological matrices prior to mass spectrometry (MS) analysis. The review covers the application of MIPs in depletion of abundant proteins, protein and proteotypic peptide capture, and capture of post-translational modifications (PTMs), as well as provides an overview of available MIP formats and mass spectrometric techniques used in protein analysis after MIP capture. Overall, the review highlights that MIPs for sample clean-up in mass spectrometric protein and proteomics analysis from biological matrices is still not fully matured.
Article
Multidisciplinary Sciences
Huan Bao Ngo, Inger Oulie, Leon Reubsaet, Trine Gronhaug Halvorsen
Summary: This paper presents a protocol for efficient sample cleanup of low-abundance proteins using bead-based proteolysis and LC-MS/MS determination. It also includes detailed procedures for bead preparation and determination of biomarkers in dried serum. Results may vary depending on the sampling material, with higher signal intensities observed for samples collected using VAMS compared to DSSs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Chemistry, Analytical
Hanne Bendiksen Skogvold, Helge Rootwelt, Leon Reubsaet, Katja Benedikte Presto Elgstoen, Steven Ray Wilson
Summary: Dried blood spot samples offer a simple and safe option for diagnostics, with liquid chromatography-mass spectrometry being a versatile measurement tool. These samples provide valuable information for metabolomics, xenobiotic analysis, and proteomics. Targeted analysis of small molecules is the main application, but there are emerging applications in untargeted metabolomics and proteomics. Applications range from newborn screening to disease diagnostics and monitoring, as well as research into the effects of diet, exercise, xenobiotics, and doping. A variety of dried blood spot products and liquid chromatography-mass spectrometry methods are available, including novel approaches like on-paper sample preparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Samira Dowlatshah, Frederik Andre Hansen, Chen Zhou, Maria Ramos-Payan, Trine Grongaug Halvorsen, Stig Pedersen-Bjergaard
Summary: Electromembrane extraction (EME) of peptides involves the transfer of positively charged peptides from an aqueous sample through a liquid membrane into an aqueous acceptor solution under an electrical field. However, the use of ionic carriers in the liquid membrane increases the system's conductivity and leads to instability and drifting pH. In this study, a selection of organic solvents without ionic carriers was tested as pure liquid membrane for EME of peptides, and tri(pentyl) phosphate was found to be the most suitable solvent. It extracted peptides with a net charge of +1 and no more than two polar side chains, and was able to extract leu-enkephalin, met-enkephalin, and endomorphin from human blood plasma.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: In 2017, a smart sampling technique was introduced, allowing for sample preparation of proteins to begin at the moment of sampling, reducing the need for extensive sample preparation in the lab. This approach reshuffles the conventional protein analysis workflow, simplifying the sample preparation steps required upon sample arrival in the lab.
ANALYTICAL SCIENCE ADVANCES
(2023)