Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Peiqi Luo, Liping Wang, Lixia Jiang, Jie Sun, Yafeng Li, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: Graphdiyne, a new carbon nanomaterial, has been utilized as a matrix in laser desorption ionization mass spectrometry for small molecule analysis, showing enhanced desorption and ionization efficiency. The use of Graphdiyne in LDI MS analysis can potentially improve the early diagnosis of liver cancer by identifying fatty acids as potential biomarkers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Instruments & Instrumentation
Maximilian Heide, Carsten Engelhard
Summary: Using a flowing atmospheric-pressure afterglow (FAPA) source to probe analytes on tailored thin-layer chromatography (TLC) plates enhances signal and improves quantification capabilities in plasma-based ambient desorption/ionization mass spectrometry (ADI-MS). Quantitative results can be achieved by using the TLC plate as a sampling plate without a preceding separation step. Modified dimethyl (RP2) and cyano (CN) silica TLC materials showed superior performance compared to other TLC materials.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Marcos Bouza, Norman Ahlmann, Juan F. Garcia-Reyes, Joachim Franzke
Summary: The present study investigated the potential of SALD-FμTP-MS as a rapid analytical technique for direct analysis of surface-deposited samples. Paper was used as the substrate and an infrared hand-held laser was employed for sample desorption. The optimization process involved the use of viscous solvents to enhance desorption and ionization efficiencies. Ethylene glycol (EG) was identified as a suitable solvent, improving sensitivity and substrate stability. The applicability of SALD-FμTP-MS was demonstrated by successfully analyzing food, water, and biological samples, particularly for thermolabile and polarity diverse compounds.
ANALYTICAL CHEMISTRY
(2023)
Review
Spectroscopy
Wendy H. Mueller, Alexandre Verdin, Edwin De Pauw, Cedric Malherbe, Gauthier Eppe
Summary: Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a technique that uses nanostructured substrates to promote the desorption/ionization of analytes, allowing for both untargeted and targeted analysis. This review focuses on SALDI-MS imaging (SALDI-MSI), discussing the nomenclature, fundamental mechanisms, analytical strategies, and applications in various fields. SALDI-MSI has strengths and limitations, but shows promising prospects for future development.
MASS SPECTROMETRY REVIEWS
(2022)
Review
Chemistry, Physical
Wen Ma, Jun Li, Xianjiang Li, Yu Bai, Huwei Liu
Summary: The rapid advancement of nanotechnology in the field of SALDI-MS has led to the development of novel nanoparticles and nanostructured substrates, improving detection sensitivity and specificity, and impacting the field of biomedical analysis significantly.
Article
Chemistry, Analytical
Yuka Fujito, Yoshihiro Izumi, Kohta Nakatani, Masatomo Takahashi, Yoshihiro Hayakawa, Mitsuo Takayama, Takeshi Bamba
Summary: Supercritical fluid chromatography (SFC) is often coupled with electrospray ionization mass spectrometry (ESIMS) for analyte detection. The ionization mechanism in SFC/ESI-MS is different from liquid chromatography due to the use of CO2 as a mobile phase. The formation of methoxylcarbonic acid contributes to ion generation and detection in positive-ion mode, but interferes with ionization in negative-ion mode.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Multidisciplinary
Yajie Ding, Congcong Pei, Weikang Shu, Jingjing Wan
Summary: Metabolic analysis in biofluids plays a crucial role in disease diagnosis and monitoring, and LDI MS-based metabolic analysis shows great potential in this field. This review discusses the essential elements of LDI MS-based metabolic analysis and summarizes the recent developments in diagnostic applications using different nanomaterials and machine learning algorithms.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Analytical
Lea Ledoux, Yanis Zirem, Florence Renaud, Ludovic Duponchel, Michel Salzet, Nina Ogrinc, Isabelle Fournier
Summary: This study directly compared water-assisted laser desorption ionization (WALDI) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging, with MALDI as the benchmark for label-free molecular tissue analysis in biomedical research. The results showed that the two techniques are very similar in lipidomic analysis, especially when using norharmane MALDI matrix in negative ion mode. This similarity allows for the integration of data from both techniques to increase dataset and improve classification models.