期刊
JOURNAL OF SEPARATION SCIENCE
卷 31, 期 13, 页码 2457-2462出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800032
关键词
anthraquinoids; CE; flavonoids; MS; natural dyes
CE with UV-Vis and MS detections was investigated as a technique for detection of main components of selected natural dyes of plant and insect origin. The BGE giving the best separation of the investigated flavonoids and anthraquinoids, suitable for MS detection consisted of 40 mM ammonium acetate solution of pH 9.5 with 40% ACN. LODs obtained with MS detection were even one order of magnitude lower than the ones obtained with UV-Vis detection. Application of MS detection enabled determination of eleven dye compounds from three different chemical groups in 15 min. and proved to be more satisfactory than diode-array detection in the electrophoretic analysis of main classes of natural dyes both in terms of selectivity and sensitivity of analysis.
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