Article
Chemistry, Analytical
Petr Kozlik, Jana Vaclova, Kveta Kalikova
Summary: In this study, three recently developed HILIC columns were characterized in detail to compare their peptide retention mechanisms, revealing a multimodal retention mechanism in systems with these stationary phases. By balancing the properties of analytes and stationary phases, satisfactory retention and peak shape of peptides can be achieved. Buffer concentration and pH optimization was confirmed to be an effective tool for manipulating retention and peak symmetry, enhancing the potential applications of these columns in the analysis of diverse compounds.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang
Summary: In this study, spherical silica was modified with imidazole, amide, and sulfonic acid. The modified silica was used to create novel polymerization stationary phases for hydrophilic interaction liquid chromatography (HILIC). Various characterization techniques were employed to analyze the stationary phases, and their performance was evaluated using high performance liquid chromatography. The HILIC stationary phases exhibited excellent retention properties and selectivity compared to the conventional C18 column. They were successfully applied for the separation of sulfonamides, flavonoids, and Fructus Ligustri Lucidi extracts. Overall, the prepared surface polymeric stationary phases showed great potential for the analysis of polar components and complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Matthew J. Sorensen, Mellie June Paulines, Todd D. Maloney
Summary: This study evaluated the orthogonality between ion-pair reversed phase (IP-RP), anion exchange (AEX), and hydrophilic interaction liquid chromatography (HILIC) for the separation of oligonucleotides. The results showed that coupling HILIC with either AEX or IP-RP provided the highest degree of orthogonality.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Jing Li, Danye Qiu, Feng Li, Jingwu Kang
Summary: A method was reported for preparing a poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate copolymerization)-based monolithic capillary column for the separation of polar small molecular weight compounds with nano-liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by copolymerization of N-vinylpyrrolidone and pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The polymerization solution was optimized for column permeability, theoretical plate number, asymmetric factor, and retention factor. The N-vinylpyrrolidone-based hydrophilic interaction chromatography stationary phase showed satisfactory separation selectivity for polar test analytes.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Polymer Science
Chengpeng Wei, Weisheng Yu, Liang Wu, Xiaolin Ge, Tongwen Xu
Summary: In this study, a series of physically and chemically stable anion exchange membranes (AEMs) with desirable properties were synthesized. The AEMs exhibited remarkable OH- conductivity and alkaline stability, as well as good mechanical properties and dimensional stability. The rational structural design of these AEMs provides inspiration for the development of high-performance membranes.
Article
Biochemical Research Methods
Lidia Redon, Xavier Subirats, Marti Roses
Summary: The study used pycnometric and homologous series retention methods to investigate the volume and mean composition of water-rich layers partially adsorbed on the surface of HILIC column fillings with acetonitrile-water and methanol-water as eluents. The findings suggest that water is preferentially adsorbed on the surface of the HILIC bonded phase in hydroorganic eluents with high acetonitrile or methanol concentrations, forming several water-rich transition layers. In eluents with low acetonitrile or methanol concentrations, the amount of preferentially adsorbed water is minimal, and the retention behavior is similar to that of reversed-phase liquid chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Tohru Ikegami, Ashin Taniguchi, Tomoyuki Okada, Kanta Horie, Syuntaro Arase, Yuka Ikegami
Summary: Commercially available HILIC columns were characterized based on their ability to distinguish structural differences between small molecules in complex matrices. PCA and cluster analysis were used to classify the columns into two main groups with further subdivisions based on stationary phase functionalities. The selectivity of columns for different functional groups and the impact of factors such as hydrophilicity and pH conditions were assessed through analytical tests, contributing to improved column selection for HILIC separations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen
Summary: An analytical method using HPLC with diode array and evaporative light scattering detectors was established for the simultaneous determination of hydrophobic and hydrophilic compounds in the dried rhizome of Atractylodes macrocephala Koidz. The method provides a new choice for the quality control of traditional Chinese medicines and health foods.
Article
Biochemistry & Molecular Biology
Pavel Jandera, Tomas Hajek
Summary: Polar columns in HILIC systems absorb water from the mixed aqueous-organic mobile phases, forming a diffuse layer that becomes part of the stationary phase and plays a dominant role in retaining polar compounds. Adopting a convention that considers the absorbed water as part of the stationary phase volume allows for unambiguous determination of phase volumes and thermodynamically correct chromatographic data. Experimental methods combined with Karl Fischer titration can determine the volume of the aqueous stationary phase, enabling prediction of retention effects and more accurate determination of phase volumes in columns.
Article
Engineering, Environmental
Xu Dong, Yiren Lu, Zhengyu Wu, Xianhua Liu, Yindong Tong
Summary: Understanding photochromism is crucial for developing new ways to modulate the optical responses of materials. In this study, ammonium tungsten bronze (NH4)xWO3 with a unique flower-like hierarchical structure was prepared, leading to a significant advance in photochromism. The large surface area exposed more photoactive sites, resulting in a high color difference. The study also revealed the essential surface structure-breaking behaviors of hydrogen bonds between NH4+ and tunneled WO3 frameworks. Furthermore, the energy-saving window based on hydrogen ammonium tungsten bronze coating showed great potential for reducing indoor air temperature and temperature difference between various indoor areas.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Martin Gilar, Brooke M. Koshel, Robert E. Birdsall
Summary: This study investigated the separation of a fully phosphorothioated oligonucleotide from its truncated n-1 species and selected phosphodiester impurities using ion-pair reversed-phase chromatography. The hydrophobicity of ion-pairing agents impacts the separation selectivity of n-1 species. The study found that hydrophilic interaction chromatography provided a different retention pattern and improved the chromatographic separation of the truncated and phosphodiester impurities.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Nanoscience & Nanotechnology
Ryan Kingsbury, Maruti Hegde, Jingbo Wang, Ahmet Kusoglu, Wei You, Orlando Coronell
Summary: In this study, we successfully synthesized a series of anion exchange membranes containing non-covalent cross-links formed by a hydrogen bond donor and acceptor. These monomers act synergistically to improve both membrane permselectivity and conductivity, and can be used to tune these properties relatively independently of each other, escaping the tradeoff observed in conventional membranes.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Martin Gilar, Kenneth D. Berthelette, Thomas H. Walter
Summary: By comparing 19 different hydrophilic interaction chromatography columns, it was found that the selectivity differences strongly depended on ionic interactions. Columns with neutral stationary phases showed more similar selectivity compared to those with cation or anion exchange activity. Zwitterionic columns exhibited some degree of cation or anion exchange activity, but to a lesser extent than silica and amine-containing phases.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Ruth Rieser, Johanna Koch, Greta Faccioli, Klaus Richter, Tim Menzen, Martin Biel, Gerhard Winter, Stylianos Michalakis
Summary: Recombinant adeno-associated virus (rAAV) vectors are a promising technology for gene therapy, showing potential in treating genetic disorders. Efficient production and purification methods are in high demand for rAAVs, and liquid chromatography-based strategies, including combinations of different chromatography techniques, offer promise for achieving highly pure rAAVs.
Article
Biochemical Research Methods
Jose Antonio Navarro-Huerta, Amarande Murisier, Jennifer M. Nguyen, Matthew A. Lauber, Alain Beck, Davy Guillarme, Szabolcs Fekete
Summary: The study evaluated the potential of ultra-short column hardware for ion exchange chromatography, comparing various prototype and commercial columns with different lengths and packing materials. The selected column with dimensions of 15 x 2.1 mm allowed for very fast separations of therapeutic proteins and fragments, demonstrating a compromise between efficiency and analysis time for high-throughput separations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
