Review
Biochemistry & Molecular Biology
Shaojuan Huang, Ming Dong, Qiang Chen
Summary: Breast cancer metastasis, which is the leading cause of death in breast cancer patients, is a complex process involving multiple physiological changes. Recent studies have shown that exosomes, membrane-bound extracellular vesicles, play a crucial role in cancer metastasis. In this review, the role of breast cancer-derived exosomes in metastasis organotropism is summarized, and the potential clinical applications of targeting exosomes for breast cancer diagnosis and therapy are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Elena Arance, Viviana Ramirez, Alejandro Rubio-Roldan, Francisco M. Ocana-Peinado, Catalina Romero-Cachinero, Ana Belen Jodar-Reyes, Fernando Vazquez-Alonso, Luis Javier Martinez-Gonzalez, Maria Jesus Alvarez-Cubero
Summary: This study evaluated the application of non-invasive biomarkers in liquid biopsy for renal cell carcinoma, focusing on exosomes and mitochondrial DNA (mtDNA) as promising and stable markers. The findings suggested that the F fraction, which contains both exosome data and mitochondrial markers, could serve as a powerful biomarker for susceptibility to RCC. Additionally, mtDNA showed significance in evaluating RCC aggressiveness.
Review
Oncology
Evelyn Lattmann, Mitchell P. Levesque
Summary: Melanoma, the deadliest form of skin cancer, can be diagnosed and treated using extracellular vesicles as biomarkers, which also reveal important signaling pathways for cancer progression.
Review
Biochemistry & Molecular Biology
Zhichen Jiang, Huiju Wang, Yiping Mou, Li Li, Weiwei Jin
Summary: This review paper provides a detailed description of the roles and mechanisms of exosomes in pancreatic cancer, emphasizing their potential clinical applications in the treatment of pancreatic cancer.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Immunology
Seyedeh Alia Moosavian, Maryam Hashemi, Leila Etemad, Sara Daneshmand, Zahra Salmasi
Summary: Exosomes play a crucial role in early diagnosis and various functions related to angiogenesis, immune modulation, migration, metastasis, chemotherapy resistance, and tumor progression in melanoma. Natural exosomes have the potential to be utilized as nanocarriers and cancer vaccines for more efficient cancer treatment in the era of novel bioengineering and immunotherapy approaches.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Medicine, Research & Experimental
Chu-An Wang, Shaw-Jenq Tsai
Summary: This article discusses the two pathways of cancer metastasis, particularly through the lymphatic system, and how cancer cells regulate this process through extracellular vesicle-dependent lymphangiogenesis.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Vivek P. Chavda, Vrashabh V. Sugandhi, Chandrakantsing V. Pardeshi, Rahul Jaywant Patil, Mit Joshi, Bhoomika Patel, Avinash Khadela, Rajashri Bezbaruah, Bedanta Bhattacharjee, Pankti C. Balar, Lalitkumar K. Vora
Summary: Exosomes are multivesicular bodies that release their cargo into surrounding body fluids by fusing with the plasma membrane. They transfer various biomolecules between cells, making them potential therapeutic drug delivery systems. The use of exosomes for cancer detection and prognosis has gained considerable interest. In addition to existing engineering approaches, novel methodologies for exosomal alteration show promise in advancing therapeutic medicines for cancer. This review highlights the theranostic potential of exosomes in various types of cancer.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Marian Cruz-Burgos, Sergio A. Cortes-Ramirez, Alberto Losada-Garcia, Miguel Morales-Pacheco, Eduardo Martinez-Martinez, Jorge Gustavo Morales-Montor, Alejandro Servin-Haddad, J. Samuel Izquierdo-Luna, Griselda Rodriguez-Martinez, Maria del Pilar Ramos-Godinez, Vanessa Gonzalez-Covarrubias, Abraham Canavera-Constantino, Imelda Gonzalez-Ramirez, Boyang Su, Hon S. Leong, Mauricio Rodriguez-Dorantes
Summary: Metastasis is the main cause of death in prostate cancer patients. This study investigates the potential diagnostic value of extracellular vesicle-derived microRNAs in liquid biopsies for prostate cancer. Through microarray analysis, differential expression of microRNAs was observed in metastatic lymph nodes compared to prostate tumors and non-metastatic lymph node tissues. Specifically, miR-150-5p shows promise as a diagnostic marker for high-grade Gleason scores and early detection of metastasis.
Review
Oncology
Naoomi Tominaga
Summary: Cell-cell communication through extracellular vesicles (EVs) plays a crucial role in cancer malignancy mechanisms, with potential applications in liquid biopsy diagnostics and drug delivery systems for novel cancer treatments.
Review
Andrology
Fumihiko Urabe, Takahiro Kimura, Kagenori Ito, Yusuke Yamamoto, Shunsuke Tsuzuki, Jun Miki, Takahiro Ochiya, Shin Egawa
Summary: Urinary extracellular vesicles are considered a potential source of biomarkers for bladder cancer, as they are easy to collect and can reflect the current state of the disease.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rafal Szatanek, Monika Baj-Krzyworzeka
Summary: Cancer metastasis involves complex interactions between various cell types and the tumor microenvironment, with tumor cells releasing proteins and nucleic acids through extracellular vesicles to facilitate dissemination. This process plays a crucial role in tumor progression and escape from immune surveillance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Premanshu Kumar Singh, Aarti Patel, Anastasia Kaffenes, Catherine Hord, Delaney Kesterson, Shaurya Prakash
Summary: This article provides a tutorial review of the existing microfluidic technologies for cancer diagnostics, with a focus on extracellular vesicle isolation methods. Extracellular vesicles have generated significant interest as biomarker cargo holders for cancer diagnosis. However, the isolation and purification of these vesicles remain challenging. Technological advances in microfluidics have the potential to overcome these challenges and enable point-of-care diagnostics for cancer.
Article
Biochemistry & Molecular Biology
Hilal A. Rather, Shalini Mishra, Yixin Su, Ashish Kumar, Sangeeta Singh, Biswapriya B. Misra, Jingyun Lee, Cristina M. Furdui, Lindsey R. Hamilton, Robert W. Gould, Susan H. Nader, Michael A. Nader, Gagan Deep
Summary: This study investigates the effects of prenatal cocaine use on postnatal development and long-term neurobiological outcomes. The researchers found that extracellular vesicles (EVs) could potentially serve as biomarkers to assess the adverse effects due to prenatal cocaine exposure.
Review
Cell Biology
Marta Prieto-Vila, Yusuke Yoshioka, Takahiro Ochiya
Summary: Extracellular vesicles (EVs), particularly those carrying mRNAs, play crucial roles in supporting tumor progression, inducing microenvironmental remodeling, and promoting metastasis in cancer. Moreover, EVs carrying mRNAs have potential applications as noninvasive diagnostic tools and novel therapeutic approaches.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Emily Clement, Ikrame Lazar, Camille Attane, Lorry Carrie, Stephanie Dauvillier, Manuelle Ducoux-Petit, David Esteve, Thomas Menneteau, Mohamed Moutahir, Sophie Le Gonidec, Stephane Dalle, Philippe Valet, Odile Burlet-Schiltz, Catherine Muller, Laurence Nieto
Article
Nanoscience & Nanotechnology
Cecilia Boyon, Vanessa Soldan, Michel Mitov
Summary: The chitin-based cuticle of beetles with twisted structures have specific optical characteristics that can be transcribed into cholesteric liquid-crystal oligomers under experimental conditions, which can be time-tunable in color with thermal annealing. By observing the internal morphology with transmission electron microscopy, different spectral bands and reflection colors can be achieved. These materials can be used to create smart reflective labels that record the product conservation history and temperature information.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Ali Khateb, Anagha Deshpande, Yongmei Feng, Darren Finlay, Joo Sang Lee, Ikrame Lazar, Bertrand Fabre, Yan Li, Yu Fujita, Tongwu Zhang, Jun Yin, Ian Pass, Ido Livneh, Irmela Jeremias, Carol Burian, James R. Mason, Ronit Almog, Nurit Horesh, Yishai Ofran, Kevin Brown, Kristiina Vuori, Michael Jackson, Eytan Ruppin, Aniruddha J. Deshpande, Ze'ev A. Ronai
Summary: Epigenetic changes play a role in AML tumorigenesis, with ubiquitin ligase RNF5 and its substrate RBBP4 influencing AML development by regulating transcription and affecting sensitivity to HDAC inhibitors. Inhibiting RNF5 can reduce AML cell growth and prolong survival in mouse models, highlighting a potential therapeutic target for stratifying patients for HDAC inhibitor treatment.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Philippe Nicolas, Jocelyn Ollier, Daiki Mori, Guillaume Voisinne, Javier Celis-Gutierrez, Claude Gregoire, Jeanne Perroteau, Regine Vivien, Mylene Camus, Odile Burlet-Schiltz, Anne Gonzalez de Peredo, Beatrice Clemenceau, Romain Roncagalli, Henri Vie, Bernard Malissen
Summary: Using traceable gene tagging, we investigated the composition and dynamics of TCR-induced signalosomes in human T cells. We found a high degree of conservation in the proximal TCR-signaling network between human CD4(+) and CD8(+) T cells, as well as between human and mouse T cells. Our study suggests that drugs targeting the proximal TCR signaling network should behave similarly when applied to human and mouse T cells. However, differences likely exist in the distal TCR-signaling pathway, and our fast-track AP-MS approach can be favored to determine the mechanism of action of drugs targeting human T cell activation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Dermatology
Ikrame Lazar, Emily Clement, Lorry Carrie, David Esteve, Stephanie Dauvillier, Mohamed Moutahir, Stephane Dalle, Veronique Delmas, Nathalie Andrieu-Abadie, Lionel Larue, Catherine Muller, Laurence Nieto
Summary: Obesity is associated with the occurrence and development of melanoma. The mechanism involves the downregulation of p16(INK4A) expression in melanoma cells by adipocytes through beta-catenin-dependent regulation, which increases cell motility. Adipocytes from individuals with obesity secrete a larger number of vesicles, delivering more beta-catenin to melanoma cells.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ikrame Lazar, Bertrand Fabre, Yongmei Feng, Ali Khateb, Philippe Frit, Anna Kashina, Tongwu Zhang, Emily Avitan-Hersh, Hyungsoo Kim, Kevin Brown, Ivan Topisirovic, Ze'ev A. Ronai
Summary: ATE1 is overexpressed in NRAS-mutant melanomas and downregulated in BRAF-mutant melanomas. Decreased ATE1 expression reduces the aggressiveness of melanoma cells and affects their response to drugs and serum deprivation. ATE1 may play a role in regulating the function of AXIN1 in melanoma.
Article
Multidisciplinary Sciences
S. Cohen, A. Guenole, I Lazar, A. Marnef, T. Clouaire, D. V. Vernekar, N. Puget, V Rocher, C. Arnould, M. Aguirrebengoa, M. Genais, N. Firmin, R. A. Shamanna, R. Mourad, V. A. Bohr, V Borde, G. Legube
Summary: This study reveals that accumulation of RNA:DNA hybrids at transcriptionally active loci induces DNA synthesis and cell toxicity. Bloom RecQ DNA helicase (BLM) plays a critical role in repair process and is recruited in a transcription-dependent manner.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Michel Mitov, Cecilia Boyon, Vanessa Soldan
Summary: This study demonstrates the development of adaptive monolayers with distinct coexisting colors through controlling the local anisotropy of surface tension. The reflection wavelength is tuned by adjusting the helical pitch, and the internal nanoscale structure is verified by transmission electron microscopy. The surface design of these films allows for their bulk chiral self-organization on various substrates. Potential applications include autonomous vehicles, functional facades, robot camouflage skins, and anticounterfeiting labels.
ACS APPLIED NANO MATERIALS
(2022)
Article
Multidisciplinary Sciences
Amelie Bonaud, Laetitia Gargowitsch, Simon M. Gilbert, Elanchezhian Rajan, Pablo Canales-Herrerias, Daniel Stockholm, Nabila F. Rahman, Mark O. Collins, Hakan Taskiran, Danika L. Hill, Andres Alloatti, Nagham Alouche, Stephanie Balor, Vanessa Soldan, Daniel Gillet, Julien Barbier, Francoise Bachelerie, Kenneth G. C. Smith, Julia Jellusova, Pierre Bruhns, Sebastian Amigorena, Karl Balabanian, Michelle A. Linterman, Andrew A. Peden, Marion Espeli
Summary: We identified SNARE Sec22b as a critical regulator of plasma cell maintenance and function. In the absence of Sec22b, plasma cells were hardly detectable and serum antibody titers were dramatically reduced, leading to a failure in mounting a protective immune response. Mechanistically, Sec22b contributes to efficient antibody secretion and is involved in regulating plasma cell transcriptional identity, as well as the morphology of the endoplasmic reticulum and mitochondria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Laureen Moreaud, Sebastien Viollet, Agathe Urvoas, Marie Valerio-Lepiniec, Agnes Mesneau, Ines Li de la Sierra-Gallay, Jessalyn Miller, Malika Ouldali, Cecile Marcelot, Stephanie Balor, Vanessa Soldan, Cristelle Meriadec, Franck Artzner, Erik Dujardin, Philippe Minard
Summary: A versatile strategy for creating inducible protein assembly with predefined geometry is demonstrated in this article. The assembly is triggered by a binding protein that staples two identical protein bricks together in a predictable spatial conformation. This work opens up possibilities for designing and fabricating multiscale protein origami with arbitrarily programmed shapes and chemical functions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Marie Rebeaud, Caroline Bouche, Stephanie Dauvillier, Camille Attane, Carlo Arellano, Charlotte Vaysse, Frederique Fallone, Catherine Muller
Summary: Obesity is a negative prognostic factor for breast cancer, but the underlying biological mechanisms are largely unknown. This study suggests that altered transfer of free fatty acids (FFA) between adipocytes and tumor cells might contribute to tumor progression in obese conditions. A 3D model using a fibrin matrix is introduced as an alternative for studying the interaction between mammary adipocytes (M-Ads) and cancer cells, showing FFA transfer and its amplification by obesity.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Geraud, Camille Falcou, Julien Parra, Carine Froment, David Rengel, Odile Burlet-Schiltz, Julien Marcoux, Jerome Nigou, Michel Riviere, Emeline Fabre
Summary: Protein-O-mannosyltransferase is crucial for the virulence of Mycobacterium tuberculosis, and inhibitors of this enzyme could potentially improve treatment of mycobacterial diseases. We developed a target-based phenotypic assay and identified compounds that significantly inhibit the enzyme's activity without affecting bacterial growth or mannoprotein secretion.
Review
Biochemistry & Molecular Biology
Soazig Le Lay, Sophie Rome, Xavier Loyer, Laurence Nieto
Summary: Adipocytes, the largest human energy reservoir, communicate with other cells and organs through the release of adipokines and extracellular vesicles (Ad-EVs) to regulate adipose tissue size and energy supply. Ad-EVs play a role in healthy adipose tissue remodeling and dysfunctional adipocytes can lead to inflammation and abnormal conditions through altered Ad-EV secretion and composition.
Article
Immunology
Daiki Mori, Claude Gregoire, Guillaume Voisinne, Javier Celis-Gutierrez, Rudy Aussel, Laura Girard, Mylene Camus, Marlene Marcellin, Jeremy Argenty, Odile Burlet-Schiltz, Frederic Fiore, Anne Gonzalez de Peredo, Marie Malissen, Romain Roncagalli, Bernard Malissen
Summary: The study used a CRISPR/Cas9-based platform to investigate the roles of LAT, CD5, and CD6 in TCR signal propagation in mouse T cells. It was found that LAT and CD5 signalosomes have positive and negative functions, respectively, while the CD6 signalosome contains both positive and negative regulators of T cell activation. Furthermore, CD6 was shown to play a role in inflammatory pathologies and autoimmune diseases, with potential as a therapeutic target.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)