期刊
JOURNAL OF RHEUMATOLOGY
卷 35, 期 12, 页码 2372-2375出版社
J RHEUMATOL PUBL CO
DOI: 10.3899/jrheum.070839
关键词
ANKYLOSING SPONDYLITIS; INTRACELLULAR CYTOKINES; CD4+CELLS; CD8+CELLS
类别
资金
- National Foundation for Scientific Research (OTKA) [T048541]
- Debrecen University [Mec-14/2005]
Objective. To determine the role of inflammatory mediators in the pathogenesis of ankylosing spondylitis (AS), we investigated peripheral blood lymphocyte subsets and their intracellular cytokine production. Methods. The percentages of T and B lymphocytes, natural killer (NK) cells, activated T lymphocytes, CD4+ T helper (Th), and CD8+ T cytotoxic (Tc) cells were determined by flow cytometry in 42 patients with AS compared to 52 healthy controls. In order to assess circulating Th1/Th2 and Tc1/Tc2 Subsets, we used a whole-blood cytometric assay based on the intracellular interferon-gamma, interleukin 4 (IL-4), and IL-10 expression of the cells. Results. In the peripheral blood, the frequencies of CD4+ T helper and CD56+ NK cells were higher in AS (54.8% and 16.2%, respectively) compared to controls (45.3% and 10.8%) (p < 0.05)., The frequencies of Th0 (1.9% vs 0.8%) and Tc0 (2.1 % vs 0.8%) cells were higher, while that of Tc1 cells was lower (26.6% vs 40.1%) in patients with AS versus controls (p < 0.05). The percentage of IL-10-producing Tc cells was significantly higher in AS (18.4%) versus controls (8.5%) (p < 0.05). Finally, the active phase of AS was associated with significantly lower percentage of IL-10-producing Tc cells in the peripheral blood (6.6%) compared to patients with inactive AS (23.1%). Conclusion. Our results provide further evidence for an altered T cell subset distribution and intracytoplasmic cytokine balance in AS. (First Release Nov 1 2008: 1 Rheumatol 200835:2372-5: doi: 10.3899/jrheum.070839)
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