Review
Microbiology
Joren De Ryck, Petra Van Damme, Sofie Goormachtig
Summary: The type III secretion system (T3SS) is a well-studied pathogenicity determinant used by bacteria to translocate effectors (T3Es) into host cells for immune evasion and niche establishment. This article explores different approaches to functionally characterize T3Es, including host localization studies, virulence screenings, biochemical activity assays, and large-scale omics studies. Using Ralstonia solanacearum species complex (RSSC) as a case study, the advances in these methods and understanding of effector biology are discussed. Complementary data obtained from these methods offer crucial insights into the entire function of effectors and contribute to a better understanding of phytopathogens, presenting opportunities for tackling them.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shen Cong, Jun-Zhou Li, Zheng-Zhong Xiong, Hai-Lei Wei
Summary: In this study, a pathogenic R. solanacearum strain named P380 was isolated from the tomato rhizosphere. Five out of 12 core T3Es of strain P380 were introduced into Pseudomonas syringae DC3000D36E separately to determine their functions in interacting with plants. The results showed that these five T3Es play diverse roles in plant-pathogen interactions.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Article
Plant Sciences
Jeffrey K. Schachterle, Qi Huang
Summary: This study investigated the cool-virulence mechanisms of Ralstonia solanacearum UW551 by generating transposon mutants and identifying genes related to reduced virulence at cooler temperatures. The effector gene RipS1 was found to play a role in cool-virulence and could potentially serve as a target for diagnostic tools specific to cool-virulent strains of R. solanacearum. These findings contribute to a better understanding of cool-virulence and the development of control strategies against this plant pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Yaxing Su, Yanan Xu, Hailing Liang, Gaoqing Yuan, Xiaogang Wu, Dehong Zheng
Summary: This study identified key genes required for R. solanacearum survival in tomato plants using transposon insertion sequencing technology, highlighting the importance of genes related to cell wall/membrane/envelope biogenesis, amino acid transport and metabolism, energy production and conversion, posttranslational modification, protein turnover, and chaperones in the pathogenic mechanism of this bacterium.
Article
Multidisciplinary Sciences
Xi Ou Xiao, Wenqiu Lin, Enyou Feng, Xiongchang Ou
Summary: The response of eggplant to bacterial wilt was studied by analyzing the whole-root transcriptomes and metabolomes. A total of 2,896 differentially expressed genes and 63 differences in metabolites were identified after inoculation with Ralstonia solanacearum. The results showed that phytohormones played a key role in eggplant response to bacterial wilt.
Article
Biochemistry & Molecular Biology
Peng Cao, Jialan Chen, Rongbo Wang, Mengwei Zhao, Shuangxi Zhang, Yuyan An, Peiqing Liu, Meixiang Zhang
Summary: This study confirmed the recognition of T3E RipB from Ralstonia solanacearum in tobacco, showing that RipB suppresses plant basal defense and promotes pathogen infection via the cytokinin signaling pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lei Wang, Yang Gao, Nihao Jiang, Jian Yan, Weipeng Lin, Kunzheng Cai
Summary: This study investigated the impact of silicon on the control of bacterial wilt in tomatoes and elucidated the molecular mechanisms involved. The results showed that silicon application significantly reduced the occurrence of bacterial wilt and regulated the expression of virulence-related genes in R. solanacearum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Peina Cao, Chuang Zhan, Junliang Yin, Shuangjun Gong, Dongfang Ma, Yan Li
Summary: Research has found that lincRNAs are involved in regulating tomato's resistance against R. solanacearum infection, potentially through the jasmonic acid and ethylene signaling pathways, as well as in regulating the expression of AGO protein. Some lincRNAs show positive correlation with their possible target genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Nina L. Denne, Rachel R. Hiles, Oleksandr Kyrysyuk, Anjali S. Iyer-Pascuzzi, Raka M. Mitra
Summary: The study investigated the subcellular localization of 19 T3Es from phytopathogenic bacteria and found that the effectors mainly localize to the plant cell periphery and the nucleus. The data suggest that these bacteria target a wide diversity of cellular organelles and provide a foundation for developing testable hypotheses about effector function.
Article
Plant Sciences
Li Chen, Ni Lei, Akinori Kiba, Yasufumi Hikichi, Kouhei Ohnishi
Summary: The RipS family of type III effectors in Ralstonia solanacearum plays a significant role in virulence, with RipS1, RipS4, and RipS5 being identified as the most critical effectors in eggplants. The virulence of a ripS-null mutant differed in tobacco and tomato compared to eggplant.
JOURNAL OF GENERAL PLANT PATHOLOGY
(2021)
Article
Agronomy
Ana M. Bocsanczy, Peter Bonants, Jan van der Wolf, Maria Bergsma-Vlami, David J. Norman
Summary: The study aims to investigate the host specificity of four Ralstonia pseudosolanacearum (Rps) strains by identifying candidate Type 3 Effectors (T3Es) associated with specific hosts. The results show that these strains have different host specificities and are of economic importance. This study provides a strategy to reduce the number of candidate host specificity genes in closely related strains with different hosts.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Review
Microbiology
Kenji Kai
Summary: Ralstonia solanacearum species complex (RSSC) strains are devastating plant pathogens distributed worldwide. The primary cell density-dependent gene expression system in RSSC strains is phc quorum sensing (QS), which regulates the expression of about 30% of all genes. The specificity in generating and receiving its QS signal, but their signaling pathways might not differ significantly. This review discusses the genetic and biochemical factors involved in QS signal input, the regulatory network, and the interactions with soil fungi.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Connor G. G. Hendrich, Alicia N. N. Truchon, Beth L. L. Dalsing, Caitilyn Allen
Summary: Ralstonia solancearum causes bacterial wilt disease on diverse plant hosts by invading through the roots and spreading through the xylem vessels. Nitric oxide (NO) contributes to the activation of the pathogen's virulence by inducing the expression of the T3SS regulator, hrpB, and upregulating the T3SS and effectors.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Ankita Pandey, Hayoung Moon, Sera Choi, Hayeon Yoon, Maxim Prokchorchik, Jay Jayaraman, Rajendran Sujeevan, Yu Mi Kang, Honour C. McCann, Cecile Segonzac, Chul Min Kim, Soon Ju Park, Kee Hoon Sohn
Summary: In this study, the R. solanacearum effector RipJ was identified as playing a pathogenic role in a specific eggplant species and triggering bacterial wilt resistance. Despite belonging to the YopJ family of acetyltransferases, RipJ does not require conserved residues in the acetyltransferase catalytic triad to induce immunity.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Agronomy
Qiao-Qiao Guo, Yu-Zhen Li, Hua-Bin Shi, Ao-Yun Yi, Xiao-Li Xu, Hai-Hong Wang, Xin Deng, Zhi-Bing Wu, Zi-Ning Cui
Summary: A series of mandelic acid derivatives containing 2-mercapto-1,3,4-thiazole moiety were synthesized as potential inhibitors of Ralstonia solanacearum type III secretion system (T3SS). Among them, compound F-24 significantly inhibited the transcription of hrpY gene and attenuated hypersensitive response (HR) without inhibiting bacterial growth. F-24 showed better inhibitory activity than previously reported inhibitors and had the potential to control R. solanacearum.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Anthony Perrier, Xavier Barlet, David Rengel, Philippe Prior, Stephane Poussier, Stephane Genin, Alice Guidot
ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Plant Sciences
Guillaume Dubrulle, Flora Pensec, Adeline Picot, Karim Rigalma, Audrey Pawtowski, Sophie Nicolleau, Nathalie Harzic, Patrice Nodet, Riccardo Baroncelli, Gaetan Le Floch
Article
Plant Sciences
Nasim Sedighian, S. Mohsen Taghavi, Habiballah Hamzehzarghani, Jan M. van der Wolf, Emmanuel Wicker, Ebrahim Osdaghi
Article
Microbiology
Guillaume Dubrulle, Adeline Picot, Stephanie Madec, Erwan Corre, Audrey Pawtowski, Riccardo Baroncelli, Michel Zivy, Thierry Balliau, Gaetan Le Floch, Flora Pensec
Article
Biochemistry & Molecular Biology
Rekha Gopalan-Nair, Marie-Francoise Jardinaud, Ludovic Legrand, David Landry, Xavier Barlet, Celine Lopez-Roques, Celine Vandecasteele, Olivier Bouchez, Stephane Genin, Alice Guidot
Summary: The study utilized experimental evolution to investigate the adaptive potential of Ralstonia solanacearum in overcoming the resistance of tomato cultivar Hawaii 7996. After over 300 generations of serial passaging, pathogen adaptation to the resistant cultivar's environment was observed without breakdown of plant resistance. Genomic and transcriptomic analyses revealed genetic alterations and convergent global rewiring of the virulence regulatory network in independently evolved clones.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Agronomy
Gloria V. Nakato, David J. Studholme, Guy Blomme, Murray Grant, Teresa A. Coutinho, Evans M. Were, Emmanuel Wicker, George Mahuku
Summary: Xanthomonas vasicola pv. musacearum (Xvm) has caused significant losses to banana crops in Ethiopia and other East/Central African countries, with recent studies revealing a greater genetic diversity among Xvm isolates than previously reported. The presence of new clades in Ethiopia and the detection of SL 2 in the Democratic Republic of Congo support the hypothesis that the Xanthomonas wilt epidemic in East/Central Africa has been caused by a limited set of genotypes originating from a diverse pathogen pool in Ethiopia.
Article
Biodiversity Conservation
Isabel Urbina-Barreto, Frederic Chiroleu, Romain Pinel, Louis Frechon, Vincent Mahamadaly, Simon Elise, Michel Kulbicki, Jean-Pascal Quod, Eric Dutrieux, Remi Garnier, J. Henrich Bruggemann, Lucie Penin, Mehdi Adjeroud
Summary: This study quantified shelter volumes provided by individual coral colonies using underwater photogrammetry and found that the planar area and diameter of coral colonies are satisfactory proxies for estimating shelter volume. The study revealed shelter differences at reefscapes and provided insights for conservation actions and biodiversity assessments.
ECOLOGICAL INDICATORS
(2021)
Article
Food Science & Technology
Alioune Diop, Jean-Michel Meot, Mathieu Lechaudel, Frederic Chiroleu, Nafissatou Diop Ndiaye, Christian Mertz, Mady Cisse, Marc Chillet
Summary: The study found significant color changes in mangoes during drying under different ripening conditions and harvest stages, with fruits ripened at 35 degrees C showing better color retention compared to those ripened at 20 degrees C. The management of fresh mango color had a greater impact on dried mango color than the studied parameters.
Article
Microbiology
Manon Vignassa, Jean-Christophe Meile, Frederic Chiroleu, Christian Soria, Charlene Leneveu-Jenvrin, Sabine Schorr-Galindo, Marc Chillet
Summary: This study focused on the correlation between FCR disease occurrence, fungal diversity, and environmental factors in pineapple cultivation. The research identified Fusarium ananatum as the main causal agent of FCR and revealed the complexity of the pathosystem. The study also highlighted the airborne nature of FCR as a multi-partite pathosystem involving various fungal species.
Article
Microbiology
Olivier Pruvost, Damien Richard, Karine Boyer, Stephanie Javegny, Claudine Boyer, Frederic Chiroleu, Pierre Grygiel, Evelyne Parvedy, Isabelle Robene, Veronique Maillot-Lebon, Azali Hamza, Kanta Kumar Lobin, Marc Naiken, Christian Verniere
Summary: A comprehensive study on X. citri pv. citri in the South West Indian Ocean (SWIO) region revealed a new genetically unrelated pathotype A* and diverse strains in the Seychelles. The study also showed that strains from the Seychelles formed three different clusters, indicating higher diversity compared to the Comoros and the Mascarenes.
Article
Biochemistry & Molecular Biology
Riccardo Baroncelli, Flora Pensec, Daniele Da Lio, Thais Boufleur, Isabel Vicente, Sabrina Sarrocco, Adeline Picot, Elena Baraldi, Serenella Sukno, Michael Thon, Gaetan Le Floch
Summary: This study sequenced the genome of Colletotrichum lupini isolated from white lupin in France, revealing its genome structure, pathogenicity factors, and the number of specific genes, providing important insights into the pathogenic mechanism and evolution of plant pathogens.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Microbiology
Diane Mostert, Emmanuel Wicker, Mignon M. de Jager, Saif M. Al Kaabi, Wayne T. O'Neill, Suzy Perry, Chunyu Li, Yi Ganyun, Kenneth G. Pegg, Lizel Mostert, Altus Viljoen
Summary: Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungus that causes Fusarium wilt in bananas. This study identified 14 novel Foc VCGs and 17 single-member VCGs, indicating that Foc is more diverse than previously thought.
Article
Food Science & Technology
Alioune Diop, Jean-Michel Meot, Mathieu Lechaudel, Frederic Chiroleu, Nafissatou Diop Ndiaye, Christian Mertz, Mady Cisse, Marc Chillet
Summary: This study aimed to analyze the impact of different drying procedures on the color alterations of mango slices with varying maturity degrees. The results showed that standard drying effectively preserved the color of mango slices, while wet drying had a significant adverse impact, especially on mature mangoes. Additionally, hot drying had a limited effect on immature mangoes.
Review
Microbiology
Marie Belair, Alexia Laura Grau, Julie Chong, Xubin Tian, Jiaxin Luo, Xin Guan, Flora Pensec
Summary: Botryosphaeriaceae is a family of fungi that cause decay in woody plants, particularly in viticulture. Advances in gene studies have helped us understand the pathogenicity mechanisms of these fungi, which can lead to the development of better control methods and higher grape yields.
Editorial Material
Microbiology
Xin Guan, Flora Pensec