Article
Biochemistry & Molecular Biology
Maria A. A. Surovtseva, Irina I. I. Kim, Natalia A. A. Bondarenko, Alexander P. P. Lykov, Kristina Yu. Krasner, Elena V. V. Chepeleva, Nataliya P. P. Bgatova, Alexander N. N. Trunov, Valery V. V. Chernykh, Olga V. V. Poveshchenko
Summary: In this study, corneal fibroblasts (CFs) with high proliferative potential were isolated and grown in a serum-free medium, and then reverted into keratocytes. These reverted cells maintained the morphology and functional properties of primary keratocytes, making them a potential source for tissue engineering and cell therapy of corneal pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Shenyang Li, Zekai Cui, Jianing Gu, Yini Wang, Shibo Tang, Jiansu Chen
Summary: A novel medium was developed to propagate human corneal stromal cells (hCSCs) while maintaining their physiological quality, showing potential for clinical cell therapy. The study demonstrated that intrastromal injection of hCSCs in this medium has beneficial effects on cell proliferation and potential for future therapeutic applications.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Medicine, Research & Experimental
Yewei Yin, Tu Hu, Aiqun Xiang, Yanyan Fu, Yang Zhao, Xiaoying Wu, Xiaoying Wu, Dan Wen
Summary: The surface quality of corneal stromal lenticules ablated using a femtosecond laser during SMILE surgery was suboptimal, with the front surface being smoother than the rear surface and the edge not as regular as when cut with microscissors.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell Biology
Kiranjit K. Bains, Robert D. Young, Elena Koudouna, Philip N. Lewis, Andrew J. Quantock
Summary: Imaging techniques were used to study the three-dimensional microanatomy and spatiotemporal relationships of cells, extracellular matrix, and vasculature at the corneo-scleral limbus of developing and mature chicken eyes. Results showed the development of structures similar to Palisades of Vogt, associated with characteristics of stem cells, and direct cell-cell associations between corneal epithelial and stromal cells.
Article
Ophthalmology
Yanfeng Shang, Yu Li, Zhiqun Wang, Xuguang Sun, Fengju Zhang
Summary: The study found that there is a low risk of infection when using fresh human corneal stromal lenticules from SMILE, but there is a risk of rejection on reusing them. Anhydrous glycerol preservation at -78 degrees C is an ideal method for reducing antigens without damaging the structure and function of lenticules.
JOURNAL OF REFRACTIVE SURGERY
(2021)
Article
Anatomy & Morphology
Sameh M. Farouk, Samy F. Mahmoud, Haitham Eldoumani, Khaled Shoghy, Nazema S. Abdel-Megeid, Mohamed Abdo
Summary: Through morphometric analysis using scanning electron microscopy, it was found that light cells predominate in all parts of camels' corneas except the central part. The peripheral ventral and nasal parts represent the permanent and endogenous source as well as a proliferative reserve for superficial corneal epithelial cells in dromedary camels.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Biochemical Research Methods
Tong Xin, Yanan Lv, Haoran Chen, Linlin Li, Lijun Shen, Guangcun Shan, Xi Chen, Hua Han
Summary: This article proposes a new method for serial section registration using an unsupervised optical flow network to measure feature similarity and eliminate nonlinear deformation. The optical flow network is also used to estimate and compensate for cumulative registration error, enabling the reconstruction of biological tissue structures. Experimental results demonstrate that this method effectively improves the spatial continuity of serial sections and enhances the accuracy of registration and reconstruction for biological tissue structures.
Review
Biochemistry & Molecular Biology
Elzbieta Pach, Albert Verdaguer
Summary: SEM, a powerful imaging technique, is now able to study ice at nanoscale by introducing water vapor and controlling sample temperature. Many studies have focused on using SEM to study ice nucleation, morphology, thaw, and clarify ice nucleation mechanisms as well as study ice dynamics. Research in this field aims to elucidate the present and future of SEM applied to ice studies.
Article
Chemistry, Multidisciplinary
Ryan Selhorst, Zhuohang Yu, David Moore, Jie Jiang, Michael A. Susner, Nicholas R. Glavin, Ruth Pachter, Mauricio Terrones, Benji Maruyama, Rahul Rao
Summary: Layered Transition Metal Dichalcogenides (TMDs) are important materials with a diverse range of optoelectronic properties. This study investigates the spatial tailoring of TMDs through electron-beam patterning, achieving high resolution and demonstrating potential for nanoscale functionalization. The modulated properties were found to be dependent on various parameters, and the results were confirmed through spectroscopic analysis and density functional theory modeling. This research provides a robust method for property modulation and functionalization of TMDs at the nanoscale.
Article
Chemistry, Multidisciplinary
Amelia C. Y. Liu, Timothy J. Davis, Toon Coenen, Sangeetha Hari, Lenard M. Voortman, Zhou Xu, Gangcheng Yuan, Patrycja M. Ballard, Alison M. Funston, Joanne Etheridge
Summary: Waveguide modes in chemically-grown silver nanowires on silicon nitride substrates are observed using cathodoluminescence excited by high-energy electrons. The presence of a long-range, travelling surface plasmon mode modulates the coupling efficiency of incident electron energy into the nanowires, causing oscillations in the measured cathodoluminescence. The experimental data are modeled using the theory of surface plasmon polariton modes, allowing for extraction of complex mode wavenumbers and excitation strength of the long-range surface plasmon mode.
Article
Materials Science, Multidisciplinary
Yang Zhang, Hongyu Fan, Chunjie Niu, Yunqiu Cui, Weiyuan Ni, Weifeng Liu, Gunther Benstetter, Guangjiu Lei, Dongping Liu
Summary: The study observed the microscopic surface evolution of W crystals under low-energy He+ irradiations at 1050 K using scanning electron microscopy and atomic force microscopy. It was found that the He+ irradiation caused nanoscale undulations on the W (110) surface, with regions of less undulations dominating the microevolution of W crystals. Analysis indicated that the thermodynamic instability of irradiated W crystals was attributed to He nanobubble-driven surface growth.
JOURNAL OF NUCLEAR MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Haofei Zhao, Yuchen Zhu, Huanyu Ye, Yang He, Hao Li, Yifei Sun, Feng Yang, Rongming Wang
Summary: Nanocrystals play a crucial role in material sciences and industry. Designing nanocrystals with desired structures and properties is a major challenge that requires a deep understanding of atomic-scale dynamics. In situ transmission electron microscopy (TEM), especially environmental TEM, has provided valuable insights into nanocrystals. This comprehensive review highlights the progress in the understanding of nanocrystal dynamics, including nucleation and growth, structure evolution, and dynamics in reaction conditions.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Izabela Potocka, Joanna Szymanowska-Pulka
Summary: This study examined the impact of mechanical stress on the pellicle of grass roots. Results showed that while mechanical stress caused microdamage in the epidermal layers, the pellicle played a protective role in preventing damage to the protodermal cells, thereby safeguarding the root apical meristem from negative effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Michela Relucenti, Giuseppe Familiari, Orlando Donfrancesco, Maurizio Taurino, Xiaobo Li, Rui Chen, Marco Artini, Rosanna Papa, Laura Selan
Summary: Bacterial biofilms cause infections that are often resistant to antibiotics, necessitating detailed imaging techniques for research. Scanning electron microscopy with customized protocols is preferred for ultrastructural characterization of biofilm matrix and bacterial cells in drug treatment studies, providing unparalleled image quality and resolution. Various imaging methodologies, including traditional and innovative scanning electron microscopy techniques, have been used in biofilm studies to deepen understanding of their structure.
Article
Materials Science, Multidisciplinary
Robar Arafat, Christian Koehn, Annelise Jean-Fulcrand, Tim Abraham, Georg Garnweitner, Christoph Herrmann
Summary: This study investigated the usability of Al2O3, ZrO2, and TiO2 nanoparticles in water-glycerine based nanofluids as MQL-cutting fluids in tribological tests. The physical-chemical properties and behavior of the nanofluids were assessed, and the influence of these properties on wear and particle deposition on steel workpieces was evaluated.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Ophthalmology
Janice Dias, Vasilios F. Diakonis, Michael Lorenzo, Felipe Gonzalez, Kevin Porras, Simone Douglas, Marcel Avila, Sonia H. Yoo, Noel M. Ziebarth
EXPERIMENTAL EYE RESEARCH
(2015)
Article
Ophthalmology
Janice Dias, Noel M. Ziebarth
EYE & CONTACT LENS-SCIENCE AND CLINICAL PRACTICE
(2015)
Article
Ophthalmology
Vasilios F. Diakonis, Nikita Y. Likht, Nilufer Yesilirmak, Desiree Delgado, Andreas E. Karatapanis, Yener Yesilirmak, Christopher Fraker, Sonia H. Yoo, Noel M. Ziebarth
EXPERIMENTAL EYE RESEARCH
(2016)
Editorial Material
Peripheral Vascular Disease
Tyler Laurito, Vivian Sueiras, Natasha Fernandez, Luis A. Escobar, Laisel Martinez, Fotios Andreopoulos, Loay H. Salman, Roberto I. Vazquez-Padron, Noel M. Ziebarth
JOURNAL OF VASCULAR ACCESS
(2016)
Article
Cell Biology
Laurin Marie Pacheco, Lourdes Adriana Gomez, Janice Dias, Noel M. Ziebarth, Guy A. Howard, Paul C. Schiller
Article
Engineering, Biomedical
Stefan Talu, Sebastian Stach, Vivian Sueiras, Noel Marysa Ziebarth
ANNALS OF BIOMEDICAL ENGINEERING
(2015)
Article
Ophthalmology
Noel M. Ziebarth, Janice Dias, Volkan Huermeric, Mohamed Abou Shousha, Chiyat Ben Yau, Vincent T. Moy, William W. Culbertson, Sonia H. Yoo
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2013)
Article
Materials Science, Multidisciplinary
Connor A. Verheyen, Laura Morales, Joshua Sussman, Kalina Paunovska, Vita Manzoli, Noel M. Ziebarth, Alice A. Tomei
MACROMOLECULAR MATERIALS AND ENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Jonathan M. Zuidema, Courtney M. Dumont, Joanna Wang, Wyndham M. Batchelor, Yi-Sheng Lu, Jinyoung Kang, Alessandro Bertucci, Noel M. Ziebarth, Lonnie D. Shea, Michael J. Sailor
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Ophthalmology
Wyndham More Batchelor, Bianca Maceo Heilman, Esdras Arrieta-Quintero, Marco Ruggeri, Jean-Marie Parel, Fabrice Manns, Sara Cabrera-Ghayouri, Mohammed Dibas, Noel Marysa Ziebarth
Summary: This study used atomic force microscopy microindentation to assess changes in the Young's Modulus of Elasticity of the mouse lens with age and postmortem time. It was found that immediately postmortem, old mouse lenses were significantly stiffer than young mouse lenses. However, after 18 hours of incubation, there was no measurable difference in lens stiffness between old and young mouse lenses, highlighting the importance of careful experimental control in studies involving postmortem time.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Vivian M. Sueiras, Floriane Devaux, Benjamin Smith, James Lai, Wyndham Batchelor, Nikita Y. Likht, Vincent T. Moy, Noel M. Ziebarth
Article
Biochemistry & Molecular Biology
Stefan Talu, Vivian M. Sueiras, Vincent T. Moy, Noel M. Ziebarth
Article
Ophthalmology
Cristina Labate, Marco Lombardo, Maria P. De Santo, Janice Dias, Noel M. Ziebarth, Giuseppe Lombardo
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Vivian M. Sueiras, Vincent T. Moy, Noel M. Ziebarth