Article
Biochemistry & Molecular Biology
Qi Yue, Zhaoxiang Li, Qi Zhang, Quanxin Jin, Xinyuan Zhang, Guihua Jin
Summary: In this study, potential biomarkers, risk factors, and drugs for psoriasis were identified using gene expression databases and bioinformatics analysis methods, providing new clues for the clinical diagnosis and treatment of psoriasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Jia Liang, Dong Fang, Fei Yao, Lu Chen, Zhenhua Zou, Xiangcheng Tang, Lujia Feng, Yijing Zhuang, Ting Xie, Pengxue Wei, Pengfeng Li, Huiyan Zheng, Shaochong Zhang
Summary: Photoreceptor degenerative diseases are characterized by the death of photoreceptor cells, leading to irreversible visual impairment. This study explored the ceRNA regulation network and molecular mechanisms shared between primary and secondary photoreceptor degenerations. Through RNA sequencing, shared differentially expressed lncRNAs, miRNAs, and mRNAs were identified, and a ceRNA network was constructed. The complement and coagulation cascades pathway was found to be shared, and hub genes related to the immune system process were identified. Additionally, immune therapeutical drugs targeting these hub genes were identified.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Food Science & Technology
Shitong Zeng, Lili Zhang, Peng Li, Dandan Pu, Yingjie Fu, Ruiyi Zheng, Hui Xi, Kaina Qiao, Dingzhong Wang, Baoguo Sun, Shihao Sun, Yuyu Zhang
Summary: Molecular docking and molecular dynamics simulations were used to investigate the molecular interactions between caramel-like odorants and olfactory receptors. The transmembrane regions TM-3, TM-5, and TM-6 of the receptors were found to be the main contributors to the docking. Hydrogen bonding and pi-pi stacking were identified as the key forces for stabilizing caramel-like odorants. The binding energies were positively correlated with the molecular weight of the odorants. Residues Asn155, Asn206, Ser155, Asp179, Val182, and Tyr260 played important roles in the formation of complexes. Through molecular field-based similarity analysis, 4-hydroxy-5-methylfuran-3(2H)-one and methylglyoxal were identified as the odorants that tend to bind to specific receptors, resulting in a caramel-like aroma perception. These findings provide useful insights into the perception of caramel-like odorants and their screening.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Genetics & Heredity
Yongxing Lai, Chunjin Lin, Xing Lin, Lijuan Wu, Yinan Zhao, Tingfang Shao, Fan Lin
Summary: This study identified three distinct molecular subtypes of sepsis and explored different pathways associated with metabolism, oxidative phosphorylation, autophagy regulation, and VEGF pathways among these subtypes. A diagnostic model with a 25-gene signature was proven to have high diagnostic value for sepsis. Key genes associated with sepsis molecular subtypes were identified, providing potential novel biomarkers for diagnosis and treatment of sepsis.
FRONTIERS IN GENETICS
(2022)
Article
Biochemical Research Methods
Ioan Ieremie, Rob M. Ewing, Mahesan Niranjan
Summary: In this study, a model named TransformerGO is proposed, which captures the semantic similarity between GO sets dynamically using an attention mechanism. By learning dense graph embeddings for GO terms, TransformerGO can accurately distinguish negative and positive interactions, outperforming other methods in PPI prediction.
Article
Pharmacology & Pharmacy
J. -H Xiong, J. -L Chen, J. -Y Liang, F. -F Zhang, S. -M Cheng
Summary: The objective of this study was to identify hub genes and uncover the molecular mechanisms of diabetic retinopathy (DR). Through gene expression analysis, differentially expressed genes were identified and functional enrichment analysis and protein-protein interaction network construction were performed. Finally, 10 hub genes were identified. These genes may serve as potential biomarkers and therapeutic targets for DR.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yue Wu, Lingping Yang, Xianglu Wu, Lidan Wang, Hongbo Qi, Qian Feng, Bin Peng, Yubin Ding, Jing Tang
Summary: Revealing key genes and elucidating pathogenic mechanism of PCOS is crucial for targeted clinical therapy. Integrating PCOS-associated molecules, an integrative PPMI network identified potential PCOS-associated genes and significant differences in PCOS-specific tissue. The novel gene FXR2 was found to be significantly increased in PCOS patients' ovarian granulosa cells.
Article
Cell Biology
Andrea Martins-da-Silva, Mirella Baroni, Karina Bezerra Salomao, Pablo Ferreira das Chagas, Ricardo Bonfim-Silva, Lenisa Geron, Gustavo Alencastro Veiga Cruzeiro, Wilson Araujo da Silva Jr, Carolina Alves Pereira Correa, Carlos Gilberto Carlotti Jr, Rosane Gomes de Paula Queiroz, Suely Kazue Nagahashi Marie, Silvia Regina Brandalise, Jose Andres Yunes, Carlos Alberto Scrideli, Elvis Terci Valera, Luiz Gonzaga Tone
Summary: This research identified five hub genes associated with prognosis in pediatric MB samples, which are involved in important pathways and functions, and control cell stemness and pluripotency.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Review
Multidisciplinary Sciences
Sho M. Kodera, Promi Das, Jack A. Gilbert, Holly L. Lutz
Summary: Understanding the interactions within microbial communities is crucial in microbial ecology, but it is challenging due to their complexity, dynamics, and numerous unique interactions. Microbial ecologists have to employ various approaches to simplify and conceptualize the study system, in order to gain practical insights into microbial interactions in both simplified and complex systems.
Review
Biochemical Research Methods
Michaela Areti Zervou, Effrosyni Doutsi, Pavlos Pavlidis, Panagiotis Tsakalides
Summary: The study investigates using chaos game representation and time series analysis tools for protein structural class prediction, introducing the generalized multidimensional recurrence quantification analysis and data-driven algorithms to reduce feature number and define optimal parameters. Experimental results demonstrate the method's high accuracy and lower computational cost.
Review
Biochemistry & Molecular Biology
Andres Rincon-Riveros, Duvan Morales, Josefa Antonia Rodriguez, Victoria E. Villegas, Liliana Lopez-Kleine
Summary: Noncoding RNAs play key roles in gene expression regulation by interacting with other biological molecules, and computational biology can enhance our understanding of these interactions. Experimental findings drive advancements in computational methods, which in turn provide new insights for experimental validation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Gan Qiao, Jinshan Xing, Xin Luo, Chunxiang Zhang, Jingyan Yi
Summary: In this study, new biomarkers and potential therapeutic targets for polycystic ovary syndrome (PCOS) were explored using integrated bioinformatics tools. The key gene GNB3 was identified as the most important regulatory gene in PCOS, and a compound (compound 1) was found to interact with GNB3 and affect its function. The results suggest that compound 1 could be considered as a lead compound for drug development. This study provides new insights into PCOS-related drug development.
Article
Biochemistry & Molecular Biology
Congshan Jiang, Thiago Arzua, Yasheng Yan, Xiaowen Bai
Summary: This study found that neonatal exposure to 3% sevoflurane induces abnormal lncRNA and mRNA expression profiles, leading to neurotoxicity and impaired long-term memory. The dysregulated lncRNAs/mRNAs form wide molecular networks that contribute to functional neurological disease pathways in the hippocampus, resulting in acute apoptosis and memory deficits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hua Qin, Zhengzhong Zou, David Anderson, Yu Sang, Dustin Higashi, Jens Kreth, Justin Merritt
Summary: Genome evolution is a crucial aspect of biological fitness, and for bacteria, natural competence plays a significant role in this evolution. Through a regulatory mechanism in Streptococcus mutans, the integration of various environmental stimuli into the natural competence phenotype is revealed. This study also demonstrates the evolution of critical genetic regulatory abilities in proteins, which are fundamental for transducing information in complex genetic networks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Polymer Science
Hassan Nezammahalleh, Faezeh Ghanati, Shima Rezaei, Mohsin Ali Badshah, Joobee Park, Naseem Abbas, Ahsan Ali
Summary: In the past decade, many researchers have made significant contributions to the structural and molecular characterizations of biochemical interactions using microscopic techniques. These interactions play a crucial role in the production of biomaterials and the organization of biological systems. This study reviews the advantages and disadvantages of three main branches of microscopy techniques and provides a basic understanding of their applicability in different research fields. The findings of this study could be valuable for future research on various aspects of biochemical interactions.
Editorial Material
Pharmacology & Pharmacy
Dasiel O. Borroto-Escuela, Luca Ferraro, Kjell Fuxe
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Dasiel O. Borroto-Escuela, Ramon Fores, Mariana Pita, Miguel A. Barbancho, Pablo Zamorano-Gonzalez, Natalia Garcia Casares, Kjell Fuxe, Manuel Narvaez
Summary: This study assesses the effects of GALR2 and NPYY1R agonists coadministration on sustained memory and hippocampal cell proliferation, suggesting the potential therapeutic benefits for dementia conditions and memory deficits.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Noelia Cantero-Garcia, Antonio Flores-Burgess, David Ladron de Guevara-Miranda, Antonia Serrano, Laura Garcia-Duran, Araceli Puigcerver, Kjell Fuxe, Jose Angel Narvaez, Luis Javier Santin, Zaida Diaz-Cabiale, Carmelo Millon
Summary: This study analyzed the effect of GAL(1-15) on alcohol consumption and depression treatment. The combination of GAL(1-15) and ESC significantly reduced alcohol intake and enhanced ESC's reduction of reward capacity. Additionally, GAL(1-15) reversed the adverse effects of ESC in depression-related behavioral tests and forced swimming tests in rats.
Review
Cell Biology
Miguel Perez de la Mora, Dasiel O. Borroto-Escuela, Minerva Crespo-Ramirez, Jose del Carmen Rejon-Orantes, Daniel Alejandro Palacios-Lagunas, Magda K. Martinez-Mata, Daniela Sanchez-Luna, Emiliano Tesoro-Cruz, Kjell Fuxe
Summary: Major depressive disorder (MDD) and anxiety are highly prevalent mental diseases with negative impacts on society and patients. Finding effective treatments with limited side effects is an important goal. This review focuses on the role of G-protein coupled receptors (GPCRs), such as serotonin, dopamine, galanin, and opioid heteroreceptor complexes, in regulating synaptic transmission in the limbic networks of the brain, and how understanding these mechanisms can contribute to the development of new treatments for MDD and anxiety disorders.
Article
Biochemistry & Molecular Biology
Marina Mirchandani-Duque, Miguel A. Barbancho, Alexander Lopez-Salas, Jose Erik Alvarez-Contino, Natalia Garcia-Casares, Kjell Fuxe, Dasiel O. Borroto-Escuela, Manuel Narvaez
Summary: This study evaluated the role of neuropeptide Y and galanin in hippocampal neurogenesis and found that they improve spatial memory by promoting cell proliferation and inducing neuroprotective factors. This finding provides a potential novel therapy for neurodegenerative cognitive-affecting diseases.
Article
Biochemistry & Molecular Biology
Carmelo Pirri, Caterina Fede, Lucia Petrelli, Enrico De Rose, Carlo Biz, Diego Guidolin, Raffaele De Caro, Carla Stecco
Summary: This study demonstrated for the first time that ESWs can lead to the in vitro production of hyaluronan-rich vesicles in fibroblasts derived from deep/muscular fascia, indicating their potential role in regulating and remodeling the extracellular matrix.
Article
Cell Biology
Jose Erik Alvarez-Contino, Estela Diaz-Sanchez, Marina Mirchandani-Duque, Jose Andres Sanchez-Perez, Miguel A. Barbancho, Alexander Lopez-Salas, Natalia Garcia-Casares, Kjell Fuxe, Dasiel O. Borroto-Escuela, Manuel Narvaez
Summary: This study found that intranasal infusion of GALR2 and Y1R agonists promotes neuroblast proliferation and induces the expression of the neurotrophic factor BDNF in the ventral hippocampus, resulting in antidepressant effects. These effects may be mediated by increased formation of Y1R-GALR2 heteroreceptor complexes. These findings may provide a new approach for the development of novel therapies for major depressive disorder.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Giovanna Albertin, Davide Zuccon, Diego Guidolin, Raffaele De Caro, Marco Falda, Ugo Carraro, Laura Astolfi
Summary: The Venice Marathon has gained fame and prestige over time. Female participation in the 40-year age category has increased over the years. Recreational athletes showed a decrease in running speed, while trained athletes improved their performance in the 40-year age category in both sexes. This information is useful for trainers and self-training to adjust physical activity to compensate for physiological decline.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Patrizia Ambrogini, Davide Lattanzi, Marica Pagliarini, Michael Di Palma, Stefano Sartini, Riccardo Cuppini, Kjell Fuxe, Dasiel Oscar Borroto-Escuela
Summary: This study investigates the effects of 5HT1AR-FGFR1 complex activation on hippocampal pyramidal neurons and midbrain dorsal raphe serotonergic neurons using an electrophysiological approach. The results show that in normal rats, FGFR1 agonist-induced allosteric action counteracts the ability of 5HT1AR to open GIRK channels, leading to increased neuronal firing. However, in a depression rat model, the change in 5HT1AR caused by FGFR1 does not affect the GIRK channels, except in CA2 neurons. Therefore, the reduced inhibitory ability of FGFR1 on 5HT1AR-mediated GIRK channel opening may be associated with depression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sarah Amato, Monica Averna, Diego Guidolin, Cristina Ceccoli, Elena Gatta, Simona Candiani, Marco Pedrazzi, Michela Capraro, Guido Maura, Luigi F. Agnati, Chiara Cervetto, Manuela Marcoli
Summary: The interaction between oxytocin and dopamine through facilitatory D2-OT receptor-receptor interaction is considered to play roles in social or emotional behavior and has potential therapeutic benefits. However, the potential receptor-receptor interaction in astrocytes has been neglected. This study demonstrates the presence of D2-OT receptor heterodimers in striatal astrocytes and their involvement in the control of glutamatergic synapse functioning through modulation of astrocytic glutamate release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Diego Guidolin, Cinzia Tortorella, Manuela Marcoli, Chiara Cervetto, Guido Maura, Luigi F. Agnati
Summary: It is well known that astrocytes play a significant metabolic role in the nervous tissue and provide trophic support to neurons. Additionally, evidence suggests that astrocytes are important for brain activity through signaling exchange with neurons, mediated by specific receptors and extracellular microvesicles.
Article
Neurosciences
Domenico Ribatti, Vincenzo Benagiano, Diego Guidolin
Summary: Wound healing is characterized by the formation of granulation tissue, and this process is also seen in tumor growth and neurodegenerative diseases. In both conditions, fibroblasts and astrocytes play a role in synthesizing and releasing components of the extracellular matrix, preventing the wound from healing. This article aims to compare the role of wound healing in tumor growth and neurodegenerative diseases.
Article
Medicine, General & Internal
Carmelo Pirri, Nina Pirri, Diego Guidolin, Veronica Macchi, Andrea Porzionato, Raffaele De Caro, Carla Stecco
Summary: Recent studies have found an association between thoracolumbar fascia thickness and reduced gliding in patients with lower back pain. This study aimed to measure and compare the thickness of thoracolumbar fascia in chronic non-specific lower back pain patients and healthy individuals using ultrasound imaging. The results showed that lower back pain patients had a loss of anisotropy in the thoracolumbar fascia, with a homogeneous increase in thickness and a loss of adaptability in the transverse direction.
Article
Biology
Luigi F. Agnati, Diego Guidolin, Chiara Cervetto, Guido Maura, Manuela Marcoli
Summary: This article provides a brief overview of the historical and epistemological investigations on the structure and functions of the brain, which mainly rely on the combination of chemical anatomy, microscopy techniques, and computer-assisted morphometric methods. The intermingling of these approaches has led to the emergence of a new discipline called brain connectomics, enabling in-depth studies on brain circuits and the development of new therapeutic strategies.