Review
Biochemistry & Molecular Biology
Christopher M. Massa, Ziping Liu, Sheryse Taylor, Ashley P. Pettit, Marena N. Stakheyeva, Elena Korotkova, Valentina Popova, Elena N. Atochina-Vasserman, Andrew J. Gow
Summary: The modification of protein cysteine residues by nitric oxide is important for various biological functions. The formation of stable nitrosothiols under biologically relevant conditions is discussed in this review, with a focus on the factors influencing the selective nature of this modification. The review also explores the reaction mechanisms and the impact of sources of NO and the chemical nature of potential reaction targets.
Review
Biochemistry & Molecular Biology
Hyang-Mi Lee, Ji Woong Choi, Min Sik Choi
Summary: Ischemia-reperfusion injury (IRI) is a damage process induced in hypoxic tissue when oxygen is supplied after ischemia. Restoration of reduced nitric oxide (NO) levels may alleviate reperfusion injury in ischemic organs, with the protective mechanism due to anti-inflammatory and antioxidant effects of NO, as well as regulation of cell signaling pathways. S-nitrosylation (SNO) mediates the detrimental or protective effect of NO depending on the nitrosylated target protein, which also plays a role in the IRI process.
Review
Biology
Yun-Jin Ju, Hye-Won Lee, Ji-Woong Choi, Min-Sik Choi
Summary: Excessive nitrosative stress contributes to neurodegenerative diseases by producing misfolded proteins. The relationship between S-nitrosylated proteins and the accumulation of misfolded proteins was reviewed, with a focus on parkin and PDI. Therapeutic targets for protein misfolding-associated diseases include NOS and GSNOR, in addition to S-nitrosylated target proteins.
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Agriculture, Multidisciplinary
Fan Feng, Yantao Yin, Lei Zhou, Chao Ma, Wangang Zhang
Summary: This study investigated the effects of nitric oxide (NO) and its induced protein S-nitrosylation on the structures and digestion properties of beef myofibrillar protein (MP). The results showed that GSNO treatment decreased sulfhydryl contents and increased carbonyl contents of the proteins. Additionally, the surface hydrophobicity, intrinsic fluorescence intensity, and a-helix content significantly decreased with higher GSNO concentrations. Moreover, GSNO treatments affected the gastric and gastrointestinal digestibility of MP, with different concentrations having varying effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hemant B. Badgandi, Andrzej Weichsel, William R. Montfort
Summary: Nitrophorins are heme proteins used by blood feeding insects to deliver nitric oxide (NO) to victims, leading to vasodilation and antiplatelet activity. The mechanism of SNO formation in cNP involves metal-assisted oxidation of the proximal cysteine, leading to the formation of Fe(II)-NO. Steric crowding inhibits SNO formation in mutated cNP, while a relaxed proximal site enhances SNO formation. pH dependence experiments suggest direct protonation of the proximal cysteine as the underlying mechanism for NO affinity.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Cardiac & Cardiovascular Systems
Gaynor Aguilar, Francisco Cordova, Tania Koning, Jose Sarmiento, Mauricio P. Boric, Konstantin Birukov, Jorge Cancino, Manuel Varas-Godoy, Andrea Soza, Natascha G. Alves, Patricio E. Mujica, Walter N. Duran, Pamela Ehrenfeld, Fabiola A. Sanchez
Summary: Nitric oxide, produced by eNOS, promotes leukocyte adhesion through the S-nitrosylation pathway, contrary to its known inhibitory role, potentially involving PKCf S-nitrosylation as a key regulatory step.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chiara Montali, Stefania Abbruzzetti, Arne Franzen, Giorgia Casini, Stefano Bruno, Pietro Delcanale, Sandra Burgstaller, Jeta Ramadani-Muja, Roland Malli, Thomas Gensch, Cristiano Viappiani
Summary: S-Nitrosylation of cysteine residues is an important molecular mechanism for post-translational regulation of proteins, and the sensitivity of fluorescent proteins to nitric oxide has been discovered, which is of great significance for cell biology research.
Article
Biochemistry & Molecular Biology
Benjamin Lau, Hossein Fazelinia, Ipsita Mohanty, Serena Raimo, Margarita Tenopoulou, Paschalis-Thomas Doulias, Harry Ischiropoulos
Summary: This study identified and analyzed S-nitrosocysteine residues in mouse heart proteins, with a significant enrichment of these modifications in mitochondrial proteins participating in metabolism. The major functional pathways impacted by protein S-nitrosylation include regulatory proteins in glycolysis, TCA cycle, oxidative phosphorylation, and ATP production. The meta-analysis of the proteomic data supports the hypothesis that nitric oxide signaling via protein S-nitrosylation is a regulator of cardiomyocyte metabolism to ensure optimal cardiac function.
Review
Plant Sciences
Anjali Pande, Bong Gyu Mun, Waqas Rahim, Murtaza Khan, Da Sol Lee, Geun Mo Lee, Tiba Nazar Ibrahim Al Azzawi, Adil Hussain, Chang Kil Kim, Byung Wook Yun
Summary: The interaction between nitric oxide (NO) and phytohormones is mainly influenced by NO-mediated post-translational modifications (PTMs), including direct and indirect nitrosylation. This interaction plays an important role in regulating physiological processes in plants and responding to environmental stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Cinzia Verde, Daniela Giordano, Stefano Bruno
Summary: Heme proteins exhibit dual reactivity towards nitric oxide (NO) and cysteine residues, resulting in heme-dependent nitrosylation and S-nitrosylation. This interplay between heme proteins and NO plays a crucial role in the production, scavenging, and transport of nitrogen compounds. Understanding the biological functions associated with heme nitrosylation and S-nitrosylation is important in elucidating signaling pathways.
Review
Chemistry, Analytical
Xin Wang, Wenxiu Zhou, Zhenye Gao, Xiaoyuan Lv
Summary: This review summarizes the application of mass spectrometry techniques in the analysis of protein S-nitrosylation, including sample preparation techniques and qualitative and quantitative strategies. The roles of protein S-nitrosylation in human diseases are discussed, with an emphasis on current limitations and future research directions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Gizem Kayki-Mutlu, Walter J. Koch
Summary: Cardiac diseases, especially heart failure, are the leading cause of morbidity and mortality globally. The loss of beta-adrenoceptor-mediated inotropic reserve and the elevated activities of G protein-coupled receptor kinases and beta-arrestins after heart injury/stress play significant roles in heart failure. Nitric oxide produced by NO synthase enzymes modulates GPCR regulators profoundly through S-nitrosylation, independent of its canonical signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Doria Boulghobra, Mathilde Dubois, Beatrice Alpha-Bazin, Florence Coste, Maxime Olmos, Sandrine Gayrard, Isabelle Bornard, Gregory Meyer, Jean-Charles Gaillard, Jean Armengaud, Cyril Reboul
Summary: Exercise training increases the activation of eNOS and its localization in mitochondria, leading to enhanced nitric oxide production, which may represent a key mechanism of exercise-induced cardioprotection.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sonia Simao, Rafaela Ribeiro Agostinho, Antonio Martinez-Ruiz, Ines Maria Araujo
Summary: Ras proteins, as small GTPases, play important roles in cell proliferation, migration, differentiation and survival. They are often mutated in cancer and can be modified by S-nitrosylation, which affects their cellular dynamics and signaling pathways.
Article
Cardiac & Cardiovascular Systems
Tomoya Irie, Patrick Y. Sips, Shinichi Kai, Kotaro Kida, Kohei Ikeda, Shuichi Hirai, Kasra Moazzami, Pawina Jiramongkolchai, Donald B. Bloch, Paschalis-Thomas Doulias, Antonis A. Armoundas, Masao Kaneki, Harry Ischiropoulos, Evangelia Kranias, Kenneth D. Bloch, Jonathan S. Stamler, Fumito Ichinose
CIRCULATION RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Margarita Tenopoulou, Jie Chen, Jean Bastin, Michael J. Bennett, Harry Ischiropoulos, Paschalis-Thomas Doulias
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Karthik Raju, Paschalis-Thomas Doulias, Perry Evans, Elizabeth N. Krizman, Joshua G. Jackson, Oksana Horyn, Yevgeny Daikhin, Ilana Nissim, Marc Yudkoff, Itzhak Nissim, Kim A. Sharp, Michael B. Robinson, Harry Ischiropoulos
Article
Biochemistry & Molecular Biology
Karthik Raju, Paschalis-Thomas Doulias, Perry Evans, Elizabeth N. Krizman, Joshua G. Jackson, Oksana Horyn, Yevgeny Daikhin, Ilana Nissim, Marc Yudkoff, Itzhak Nissim, Kim A. Sharp, Michael B. Robinson, Harry Ischiropoulos
Article
Cardiac & Cardiovascular Systems
Payman Zamani, Benjamin French, Jeffrey A. Brandimarto, Paschalis-Thomas Doulias, Ali Javaheri, Julio A. Chirinos, Kenneth B. Margulies, Raymond R. Townsend, Nancy K. Sweitzer, James C. Fang, Harry Ischiropoulos, Thomas P. Cappola
AMERICAN JOURNAL OF CARDIOLOGY
(2016)
Article
Cardiac & Cardiovascular Systems
Payman Zamani, Victor Tan, Haideliza Soto-Calderon, Melissa Beraun, Jeffrey A. Brandimarto, Lien Trieu, Swapna Varakantam, Paschalis-Thomas Doulias, Raymond R. Townsend, Jesse Chittams, Kenneth B. Margulies, Thomas P. Cappola, David C. Poole, Harry Ischiropoulos, Julio A. Chirinos
CIRCULATION RESEARCH
(2017)
Article
Cardiac & Cardiovascular Systems
Julio A. Chirinos, Scott R. Akers, Lien Trieu, Harry Ischiropoulos, Paschalis-Thomas Doulias, Ali Tariq, Izzah Vassim, Maheswara R. Koppula, Amer Ahmed Syed, Haideliza Soto-Calderon, Raymond R. Townsend, Thomas P. Cappola, Kenneth B. Margulies, Payman Zamani
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2016)
Article
Medicine, Research & Experimental
Wen Lin, David R. Vann, Paschalis-Thomas Doulias, Tao Wang, Gavin Landesberg, Xueli Li, Emanuela Ricciotti, Rosario Scalia, Miao He, Nicholas J. Hand, Daniel J. Rader
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Microbiology
Rodolfo Urbano, Joyce E. Karlinsey, Stephen J. Libby, Paschalis-Thomas Doulias, Harry Ischiropoulos, Helen I. Warheit-Niemi, Denny H. Liggitt, Alexander R. Horswill, Ferric C. Fang
CELL HOST & MICROBE
(2018)
Article
Biochemistry & Molecular Biology
Dongyin Guan, Ying Xiong, Patricia C. Borck, Cholsoon Jang, Paschalis-Thomas Doulias, Romeo Papazyan, Bin Fang, Chunjie Jiang, Yuxiang Zhang, Erika R. Briggs, Wenxiang Hu, David Steger, Harry Ischiropoulos, Joshua D. Rabinowitz, Mitchell A. Lazar
Article
Biochemical Research Methods
Yun-Il Lee, Daniel Giovinazzo, Ho Chul Kang, Yunjong Lee, Jun Seop Jeong, Paschalis-Thomas Doulias, Zhi Xie, Jianfei Hu, Mehdi Ghasemi, Harry Ischiropoulos, Jiang Qian, Heng Zhu, Seth Blackshaw, Valina L. Dawson, Ted M. Dawson
MOLECULAR & CELLULAR PROTEOMICS
(2014)
Article
Biochemistry & Molecular Biology
Calvin A. Henard, Timothy Tapscott, Matthew A. Crawford, Maroof Husain, Paschalis-Thomas Doulias, Steffen Porwollik, Lin Liu, Michael McClelland, Harry Ischiropoulos, Andres Vazquez-Torres
MOLECULAR MICROBIOLOGY
(2014)
Article
Microbiology
Elaine R. Frawley, Joyce E. Karlinsey, Anshika Singhal, Stephen J. Libby, Paschalis-Thomas Doulias, Harry Ischiropoulos, Ferric C. Fang
Article
Medicine, Research & Experimental
Margarita Tenopoulou, Paschalis-Thomas Doulias, Kent Nakamoto, Kiara Berrios, Gabriella Zura, Chenxi Li, Michael Faust, Veronika Yakovishina, Perry Evans, Lu Tan, Michael J. Bennett, Nathaniel W. Snyder, William J. Quinn, Joseph A. Baur, Dmitriy N. Atochin, Paul L. Huang, Harry Ischiropoulos
Article
Cardiac & Cardiovascular Systems
Payman Zamani, Deepa Rawat, Prithvi Shiva-Kumar, Salvatore Geraci, Rushik Bhuva, Prasad Konda, Paschalis-Thomas Doulias, Harry Ischiropoulos, Raymond R. Townsend, Kenneth B. Margulies, Thomas P. Cappola, David C. Poole, Julio A. Chirinos
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)