Article
Biochemical Research Methods
Bruno Bohn, Pamela L. Lutsey, Weihong Tang, James S. Pankow, Faye L. Norby, Bing Yu, Christie M. Ballantyne, Eric A. Whitsel, Kunihiro Matsushita, Ryan T. Demmer
Summary: This study compared the proteome of statin users and nonusers and found differences in proteins unrelated to lipid metabolism. Further investigation into these differences may help elucidate the pleiotropic effects of statins.
JOURNAL OF PROTEOMICS
(2023)
Article
Cell Biology
Diego Quintana-Torres, Alejandra Valle-Cao, Pablo Bousquets-Munoz, Sandra Freitas-Rodriguez, Francisco Rodriguez, Alejandro Lucia, Carlos Lopez-Otin, Alejandro Lopez-Soto, Alicia R. Folgueras
Summary: This study conducted a global plasma proteomic analysis in progeroid mouse models and found several upregulated proteins related to cardiovascular disease, the main cause of death in HGPS patients. The plasma proteome of progeroid mice exhibited an old signature, indicating accelerated aging. Furthermore, specific differences were observed in the circulating proteins between physiological and premature aging, highlighting potential biomarkers and therapeutic targets.
Review
Biochemistry & Molecular Biology
Raminta Zmuidinaite, Fady I. Sharara, Ray K. Iles
Summary: The current global trend in assisted reproduction technology is to implement elective single embryo transfers to reduce the risks of multiple pregnancies, while the development of noninvasive embryo testing techniques is crucial. Promising fields of research for noninvasive assays include cell-free DNA analysis, microscopy techniques combined with artificial intelligence, and omics analysis of the spent blastocyst media. These technologies can help differentiate morphologically similar embryos and noninvasively identify chromosomal status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wei Wei, Nicholas M. Riley, Andrew C. Yang, Joon T. Kim, Stephanie M. Terrell, Veronica L. Li, Marta Garcia-Contreras, Carolyn R. Bertozzi, Jonathan Z. Long
Summary: This study introduced a proximity biotinylation strategy to label, detect, and enrich secreted polypeptides in a cell type-selective manner in mice, resulting in a proteomic atlas of secretomes from various cell types. The research confirmed known cell type-protein pairs, identified new secreted polypeptides distinguishing between cell types, and uncovered a dynamic nutrient-dependent reprogramming of hepatocyte secretome. The strategy enables dynamic and cell type-specific dissection of the plasma proteome and intercellular signaling mediators.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joseph Gillen, Thunnicha Ondee, Devikala Gurusamy, Jiraphorn Issara-Amphorn, Nathan P. Manes, Sung Hwan Yoon, Asada Leelahavanichkul, Aleksandra Nita-Lazar
Summary: Inflammatory response is crucial for resolving infections but can also be harmful. Endotoxin tolerance is a cellular mechanism where repeated exposure to LPS can decrease cellular metabolism and affect the secretion of proteins associated with cell survival, protein metabolism, and reactive oxygen species metabolism.
Review
Immunology
Seyyed Mostafa Arabi, Mahla Chambari, Mahsa Malek-Ahmadi, Leila Sadat Bahrami, Vahid Hadi, Manfredi Rizzo, Amirhossein Sahebkar
Summary: This study conducted a systematic review and meta-analysis to evaluate the effect of statin therapy in combination with ezetimibe on CRP and hs-CRP levels. The results showed that the combination therapy significantly reduced the levels of hs-CRP and overall CRP, suggesting its potential in suppressing inflammation.
INFLAMMOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael T. Rossi, Jordan C. Langston, Narender Singh, Carmen Merali, Qingliang Yang, Salim Merali, Balabhaskar Prabhakarpandian, Laurie E. Kilpatrick, Mohammad F. Kiani
Summary: The dysregulated activation of endothelial cells (ECs) plays a key role in cytokine-induced changes observed in sepsis, leading to inflammatory signaling, leukocyte adhesion/migration, and organ damage. A comprehensive analysis of proteomic changes in mouse lung, liver, and kidney ECs following exposure to proinflammatory cytokines was conducted using in vitro and in silico analysis. The findings provide insights into the molecular changes involved in the EC response to inflammation and can support the development of drugs targeting ECs within different organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Divakara S. S. M. Uppu, Xiaogang Wang, Jean C. C. Lee
Summary: The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs). The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers. The microbial secretome modulates how the organism interacts with its environment.
Article
Microbiology
Jesus Castano, Jiwei Zhang, Mowei Zhou, Chia-Feng Tsai, Joon Yong Lee, Carrie Nicora, Jonathan Schilling
Summary: The study revealed that side chain hemicellulases in brown rot fungi are more tolerant of ROS compared to white and soft rot fungi, which may have been crucial for the evolution of brown rot fungi.
Article
Nanoscience & Nanotechnology
Runze Zhao, Xiaoyue Ning, Mengqi Wang, Huanhuan Wang, Guang Xing, Li Wang, Chengzhi Lu, Ao Yu, Yongjian Wang
Summary: The study developed a redox-responsive nanoprodrug that achieved targeted drug release to plaques and synergistic therapeutic effects of simvastatin and ticagrelor on atherosclerosis treatment, showing promising therapeutic efficacy and biosafety profile.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Perez-Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinberg
Summary: The study uncovers an important role of miR-181b in regulating vascular endothelial aging through the MAP3K3-MAPK signaling pathway, revealing potential therapeutic targets for anti-aging therapy in cardiovascular disease.
Article
Medicine, General & Internal
Mauro Vaccarezza, Francesco Maria Galassi
Summary: Despite advancements in standard care, cardiovascular diseases remain the leading cause of death worldwide. Atherosclerosis is the main culprit behind heart attacks, strokes, and peripheral vascular diseases, imposing significant burdens on life expectancy, quality of life, morbidity, and costs. Elevated cholesterol levels and inflammation have been recognized as cardiovascular risk factors, and recent research highlights the role of inflammation in determining cardiovascular risk independent of cholesterol levels and treatments.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina Mikhailovna Stakhneva, Elena Vladimirovna Kashtanova, Yana Vladimirovna Polonskaya, Eugeniia Vitalievna Striukova, Viktoriya Sergeevna Shramko, Evgeny Viktorovich Sadovski, Alexey Vitalievich Kurguzov, Ivan Sergeevich Murashov, Alexander Mikhailovich Chernyavskii, Yuliya Igorevna Ragino
Summary: Quantitative proteomics studies revealed associations between several blood proteins and the presence of unstable atherosclerotic plaques in the arteries of patients with coronary atherosclerosis, including afamin, attractin, components of the complement system, hemopexin, and haptoglobin. These findings provide insight into the potential roles of these blood proteins in the development of coronary atherosclerosis and their potential as biomarkers for further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Agnieszka Krupa, Weronika Gonciarz, Paulina Rusek-Wala, Tomasz Rechcinski, Adrian Gajewski, Zuzanna Samsel, Anna Dziuba, Agnieszka Smiech, Magdalena Chmiela
Summary: Chronic Helicobacter pylori infections and a high-fat diet may synergistically contribute to the development of proatherogenic conditions, as evidenced by the infiltration of inflammatory cells into the vascular endothelium and the decreased elasticity of arteries observed in animal models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yanna Lei, Qingsong Cui, Guang Yang, Limei Piao, Aiko Inoue, Hongxian Wu, Xiang Li, Masafumi Kuzuya, Xian Wu Cheng
Summary: The study demonstrates that pitavastatin can prevent vascular senescence and atherogenesis induced by chronic stress and high fat diet in mice, potentially by increasing GLP-1 and APN levels and suppressing plaque inflammation and oxidative stress.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Correction
Orthopedics
Marco Brioschi, Filippo Randelli, Paolo Capitani, Dario Capitani
INTERNATIONAL ORTHOPAEDICS
(2022)
Review
Spectroscopy
Beatrice Zoanni, Maura Brioschi, Alice Mallia, Erica Gianazza, Sonia Eligini, Marina Carini, Giancarlo Aldini, Cristina Banfi
Summary: This review summarizes the important roles of human serum albumin (HSA) in cardiovascular diseases (CVD) and heart failure, emphasizing the physiological functions of highly abundant plasma proteins should not be neglected.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Spectroscopy
Erica Gianazza, Beatrice Zoanni, Alice Mallia, Maura Brioschi, Gualtiero Colombo, Cristina Banfi
Summary: This narrative review highlights the potential of lipoprotein-associated proteins in cardiovascular risk assessment and emphasizes the importance of high-throughput proteomic analysis.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Sonia Eligini, Erica Gianazza, Alice Mallia, Stefania Ghilardi, Cristina Banfi
Summary: Macrophages are versatile cells that can adapt their characteristics and functions to different environments. They play important roles in maintaining homeostasis and are also involved in various human diseases, including atherosclerosis. Proteomics has been widely used to study macrophages, and analyzing the proteome of different macrophage phenotypes can provide insights into their roles in atherosclerosis and identify potential therapeutic targets. In this review, we discuss different proteomic techniques used to profile macrophage phenotypes and summarize findings that contribute to understanding macrophage functions and developing strategies to combat atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Marcos Leal Brioschi, Carlos Dalmaso Neto, Marcos de Toledo, Eduardo Borba Neves, Jose Viriato Coelho Vargas, Manoel Jacobsen Teixeira
Summary: This study proposed an infrared image-based method for febrile and subfebrile people screening to comply with the society need for alternative, quick response, and effective methods for COVID-19 contagious people screening.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Imaging Science & Photographic Technology
Flavio Leme Ferrari, Marcos Leal Brioschi, Carlos Dalmaso Neto, Carlos Roberto de Medeiros
Summary: The authors developed a new non-invasive dermatological infrared macroimaging analysis technique (MacroIR) that evaluates microvascular, inflammatory, and metabolic changes in skin and mucosal lesions. The technique combines naked eye observation, polarized light dermatoscopy (PLD), and MacroIR to compare results. Ten cases were evaluated using a smartphone coupled with a dermatoscope and a macro lens integrated far-infrared transducer into specific software. MacroIR imaging showed promise in improving dermatological diseases diagnosis.
JOURNAL OF IMAGING
(2023)
Review
Biochemistry & Molecular Biology
Erica Gianazza, Maura Brioschi, Ada Iezzi, Giuseppe Paglia, Cristina Banfi
Summary: Lipid-lowering therapies are widely used to prevent ASCVD and related mortality. Omics technologies have been employed to study the mechanisms and effects of these drugs, aiming to improve treatment efficacy and safety. Pharmacometabolomics focuses on studying the effects of drugs on metabolic pathways and the variation of treatment response. This review summarizes significant metabolomic studies on lipid-lowering therapies, including statins, fibrates, and novel drugs and nutraceutical approaches. Integrating pharmacometabolomics data with other omics approaches can help understand the biological mechanisms of lipid-lowering drugs and improve treatment efficacy while reducing side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cristina Banfi, Alice Mallia, Stefania Ghilardi, Maura Brioschi, Erica Gianazza, Sonia Eligini, Pelin Sahlen, Roberta Baetta
Summary: PCYOX1 is a regulator of adipogenesis, acting through multiple key factors. Its absence reduces adipogenesis and inflammatory markers, making it a potential adipogenic factor with therapeutic potential.
Article
Biochemistry & Molecular Biology
Maura Brioschi, Yuri D'Alessandra, Massimo Mapelli, Irene Mattavelli, Elisabetta Salvioni, Sonia Eligini, Alice Mallia, Veronica Ricci, Erica Gianazza, Stefania Ghilardi, Piergiuseppe Agostoni, Cristina Banfi
Summary: Sacubitril/Valsartan, a combination drug for heart failure treatment, reduces the levels of miR-29b-3p, miR-221-3p, and miR-503-5p in the plasma of patients with stable HF. These miRNAs have potential roles in HFrEF pathophysiology.
Article
Biochemistry & Molecular Biology
Marta Zara, Andrea Baggiano, Patrizia Amadio, Jeness Campodonico, Sebastiano Gili, Andrea Annoni, Gianluca De Dona, Maria Ludovica Carerj, Francesco Cilia, Alberto Formenti, Laura Fusini, Cristina Banfi, Paola Gripari, Calogero Claudio Tedesco, Maria Elisabetta Mancini, Mattia Chiesa, Riccardo Maragna, Francesca Marchetti, Marco Penso, Luigi Tassetti, Alessandra Volpe, Alice Bonomi, Giancarlo Marenzi, Gianluca Pontone, Silvia Stella Barbieri
Summary: This study found that the expression of CD41-CD61 in sEVs can reflect the CMR-assessed ischemic damage after STEMI, providing a new strategy for the timely identification of high-risk patients and their treatment optimization.
Article
Medicine, General & Internal
Marcelo Pastor Almada Davalos, Marcos Leal Brioschi, Samir Ezequiel da Rosa, Gabriel Carneiro Brioschi, Eduardo Borba Neves
Summary: This study explored the potential of dual infrared-visual thermography (IRVT) to identify perforator veins (PV) in asymptomatic veins. The results showed that IRVT effectively distinguishes varying degrees of vascular reflux severity. IRVT shows promise as a non-invasive, radiation-free tool to enhance PV identification.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Bruno Veloso Fracasso, Renato Bender Castro, Marcos Leal Brioschi, Tais Malysz
Summary: This study used infrared point-of-care thermography to analyze the facial temperature distribution of 24 female participants with chronic migraine. The findings revealed significant temperature asymmetry in women with right-sided unilateral headaches, particularly in the right frontal and temporal regions. However, caution is advised in interpreting these results, as further research with larger samples is needed.
JOURNAL OF CLINICAL MEDICINE
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Marcos Leal Brioschi, Carlos Dalmaso Neto, Marcos De Toledo, Mayco Anderson Guedes Maciel Moreira, Nicolas Civiero, Eduardo Borba Neves, Jose Viriato Coelho Vargas, Manoel Jacobsen Teixeira
Summary: This study proposed an infrared image-based method for screening febrile and non-febrile people with the aim to meet the society's needs for effective COVID-19 screening. By using a convolutional neural network, the algorithm developed could identify COVID-19 infected individuals who did not show a fever. The method has been proven to be a valuable tool in screening and has potential applications in air travel and public places.
ARTIFICIAL INTELLIGENCE OVER INFRARED IMAGES FOR MEDICAL APPLICATIONS AND MEDICAL IMAGE ASSISTED BIOMARKER DISCOVERY
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Denise Sabbagh Haddad, Marcos Leal Brioschi, Maria Aparecida Borsatti Luchetti, Nicolas Civiero, Mayco Anderson Moreira, Emiko Saito Arita
Summary: Inflammatory toothache is a common and debilitating condition that affects patients' quality of life and overall health. Infrared thermography, combined with artificial neural networks, can assist in the detection and accurate diagnosis of inflammatory toothache.
ARTIFICIAL INTELLIGENCE OVER INFRARED IMAGES FOR MEDICAL APPLICATIONS AND MEDICAL IMAGE ASSISTED BIOMARKER DISCOVERY
(2022)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
