Article
Environmental Sciences
Eun Hea Jho, Seong Ho Yun, Punam Thapa, Joo-Won Nam
Summary: The study investigates the changes in toxic effects of surfactants during photodegradation, showing that the generation of photoproducts from Triton X-100 may lead to higher toxic effects. D. magna and A. fischeri exhibit different sensitivities to Triton X-100 and its photodegraded products, suggesting that photodegradation can alter the relative toxic effects on different aquatic organisms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Physical
Ankur Patel, Debes Ray, Mehul Khimani, Jigisha K. Parikh, Paresh Parekh, Vijay Patel, Vinod K. Aswal, Pratap Bahadur
Summary: This study investigated the behavior of a non-ionic surfactant in the presence of a hydrophobic diol, leading to changes in cloud point, viscosity, and micelle morphology. Salt and temperature effects were found to play a role in controlling micellar transitions. The structural transitions were attributed to the hydrophobic interactions between the surfactant and diol.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Physical
Qi Liu, Jingkang Wang, Hao Wu, Shuyi Zong, Na Wang, Ting Wang, Lina Zhou, Xin Huang, Hongxun Hao
Summary: Pseudo-ternary phase diagrams of water/Triton X-100/1-pentanol/cyclohexane microemulsions were determined by measuring electrical conductivity. The structure and properties of the microemulsions were analyzed using various techniques, and the phase transitions and nanostructure sizes were discussed. The type of water in W/O microemulsion was specifically analyzed.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Engineering, Environmental
Kaibo Huang, He Liu, Jinglei He, Yongyi He, Xueqin Tao, Hua Yin, Zhi Dang, Guining Lu
Summary: This study used Ag/TiO2 for photocatalytic degradation of PBDEs in washing wastes, aiming to reuse TX-100 surfactant. The results showed that BDE-47 degradation can be achieved effectively under specific conditions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Physics, Multidisciplinary
Maria del Alba Pacheco-Blas, Cesar Soto-Figueroa, Maria del Rosario Rodriguez-Hidalgo, Luis Vicente
Summary: Molecular Dynamics simulations were conducted on Triton X-100, a nonionic surfactant, to investigate its physical properties under the influence of temperature and the addition of salt (NaCl, LiCl). The shape and size of the micelles change with increasing temperature, showing a prolate ellipsoid of revolution due to the folding of polyoxyethylene chains. The hydrodynamic radius of the micelles increases progressively with higher salt concentration. The addition of electrolyte modifies the hydration shell around PEO chains, leading to chain collapse and a decrease in the cloud point, with a greater effect observed for NaCl.
PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Rui Peng Cong, Yan Hua Guo, Jian Qin Zhou, Jian Wen Wang
Summary: In this study, the addition of Triton X-100 was found to enhance laccase production in Shiraia spp. S9 mycelium culture and facilitate the biodegradation of the micropollutant triclosan. The surfactant induced the release of reactive oxygen species, increased membrane permeability, and upregulated laccase-related gene expression, resulting in a 108-fold increase in laccase production. After 2 days of culture, the degradation and adsorption rate of triclosan reached 80.71% and 10.21%, respectively, and triclosan was almost completely removed (99.88%) from the water phase through a combination of biodegradation and cloud point extraction.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marcela R. Carrilho, Polliana M. C. Scaffa, Aline Dionizio, Talita M. O. Ventura, Marilia A. R. Buzalaf, Cristina M. P. Vidal
Summary: The study aims to analyze the differential proteins in dentin and assess the effects of tissue health state and protein extraction protocol. The findings suggest that the composition and abundance of soluble proteins in dentin depend on the tissue's physiopathological state and the protocol used for protein extraction.
JOURNAL OF DENTISTRY
(2023)
Article
Chemistry, Physical
S. M. Rafiul Islam, Malik Abdul Rub, Md. Rafikul Islam, Kamrul Hasan, Shamim Mahbub, Yousef G. Alghamdi, Abdullah M. Asiri, Shahed Rana, Md. Anamul Hoque, D. M. Shafiqul Islam
Summary: The cloud point measurement and UV-visible spectroscopic techniques were used to investigate the phase separation, assembly formation, and thermodynamic properties of the Triton X-100 (TX-100) + promethazine hydrochloride (PMH) drug mixed system. The impact of monohydroxy alcohols on the clouding development of the TX-100 + drug mixed system was also demonstrated. The critical micelle concentration of the TX-100 + PMH mixture increased with temperature. The obtained free energy values revealed the non-spontaneity of clouding and spontaneous aggregation of the TX-100 + PMH mixture. The micellization of the TX-100 + PMH mixed system was accompanied by hydrophobic interaction.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Food Science & Technology
Osama I. A. Soltan, Hanaa S. S. Gazwi, Amany E. Ragab, Magda E. Mahmoud, Ferial M. N. Fudllalah, Mesfer M. Alqahtani, Abdulrahman Alasmari, Hesham S. Ghazzawy, Dalia M. Hikal
Summary: This study aimed to improve the nutritional and healthy values of bread by fortifying it with dried potato peels (BPP) and evaluate its impact on Triton X-100-induced hyperlipidemia in male albino rats. The findings showed that BPP reduced hepatic triglycerides and total cholesterol levels while increasing high-density lipoprotein (HDL-C) levels. BPP demonstrated potential lipid-lowering and hepatoprotective actions, which were attributed to its ability to reduce oxidative damage and preserve the antioxidant systems compromised by hyperlipidemia. Additionally, BPP enhanced the expression of AMPKa, a key regulator of hepatocyte lipid metabolism, while suppressing the expression of fatty acid synthase (FAS) and acetylCoA carboxylase alpha (ACCa), as well as decreasing the expression of the adipogenic transcription factor SREBP-1c. The histopathological assessment of the liver corroborated these findings. Overall, this investigation revealed the positive impact of bread fortified with BPP in preventing hyperlipidemia.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Oncology
Yihao Wang, Fangcheng Zhang, Nanxiang Xiong, Hao Xu, Songshan Chai, Haofei Wang, Jiajing Wang, Hongyang Zhao, Xiaobing Jiang, Peng Fu, Wei Xiang
Summary: The blood-brain barrier (BBB) is an essential structure of the central nervous system (CNS), and its remodelling plays a significant role in the occurrence and development of glioma. Changes in BBB structure and function can lead to altered permeability of local microvessels, creating favorable conditions for glioma growth. Current therapeutic methods for glioma face challenges due to the effects of BBB remodelling, indicating the need for further research and development in this area.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Kun Xiao, Rennv Wang, Congyang Liu, Meini Wang, Jiameng Zhang, Wei Dong, Tao Pan
Summary: The effects of TX-100 and β-CD on the bioavailability and biodegradation of PHE were studied. It was found that β-CD solubilized less PHE but promoted biodegradation, and resulted in less elution of cells from biofilms compared to TX-100. Microscopy observation showed that β-CD treated biofilm had a more complete structure.
PROCESS BIOCHEMISTRY
(2021)
Article
Virology
Jean-Baptiste Farcet, Johanna Kindermann, Michael Karbiener, Richard Scheinecker, Otto Kostner, Thomas R. Kreil
Summary: In response to environmental concerns, the biopharmaceutical industry is transitioning to an environmentally friendly alternative detergent like Nereid, which was synthesized to have fully equivalent virus inactivation activity to TX-100.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Physical
Md. Nazrul Islam, Malik Abdul Rub, Md. Rafikul Islam, Md. Abdul Goni, Shahed Rana, Dileep Kumar, Abdullah M. Asiri, Yousef G. Alghamdi, Md. Anamul Hoque, Shariff E. Kabir
Summary: This study investigated the potential interactions between the antibiotic ceftriaxone sodium (CTS) and the surfactant triton X-100 (TX-100) in the presence and absence of various sodium based electrolytes and hydrotropic salts. The results showed that the hydrophilic interaction was significant when sodium salicylate (NaSal) was used, but not significant when sodium benzoate (NaBenz) was used. Thermodynamic variables were calculated to evaluate the nature of the interactions, and the findings may have important implications for pharmaceutical formulations and understanding drug-surfactant interactions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Ritutama Ghosh, Revathy Raveendranath, Nand Kishore
Summary: Identifying the functionalities responsible for preventing protein fibrillation is crucial for developing effective drugs against neurodegenerative diseases. The study investigated the interactions of TX-100 and MMI with lysozyme at different stages of fibrillation, highlighting the importance of hydrogen bonding and polar interactions. A comparison with other inhibitors underscored the significance of -OH and >CO functionalities in preventing fibrillation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Tajmul Hasan, Malik Abdul Rub, Md. Tuhinur R. Joy, Shahed Rana, Farah Khan, Md. Anamul Hoque, Mahbub Kabir
Summary: The cloud point of the mixture of aqueous triton X-100 and metformin hydrochloride was studied in the presence of various inorganic salts. The values of cloud point decreased as the concentration of electrolytes increased, indicating enhanced aggregation. Thermodynamic variables were determined, revealing that hydrophobic and electrostatic interactions are the primary forces between triton X-100 and metformin hydrochloride.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Sarah Barelier, Romain Avellan, Giri Raj Gnawali, Patrick Fourquet, Veronique Roig-Zamboni, Isabelle Poncin, Vanessa Point, Yves Bourne, Stephane Audebert, Luc Camoin, Christopher D. Spilling, Stephane Canaan, Jean-Francois Cavalier, Gerlind Sulzenbacher
Summary: We report the synthesis of new CyC alkyne-containing inhibitors (CyCyne) and their use for the direct fishing of target proteins in M. tb culture via bio-orthogonal click-chemistry activity-based protein profiling (CC-ABPP). This approach led to the capture and identification of a variety of enzymes, including HsaD, which is required for the survival of M. tb within macrophages and is a potential therapeutic target. The specificity of HsaD inhibition by the CyC analogues was confirmed through biochemical and structural approaches.
Article
Biochemistry & Molecular Biology
Anne-Sophie Paguet, Ali Siah, Gabriel Lefevre, Sophie Moureu, Thierry Cadalen, Jennifer Samaillie, Franck Michels, Barbara Deracinois, Christophe Flahaut, Harmony Alves Dos Santos, Audrey Etienne-Debaecker, Caroline Rambaud, Sylvie Chollet, Roland Molinie, Jean-Xavier Fontaine, Christophe Waterlot, Marie-Laure Fauconnier, Sevser Sahpaz, Celine Riviere
Summary: This study investigates the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. The results show that wild hops exhibit high levels of genetic and chemical diversity, making them an important resource for the species.
Article
Cell Biology
Marie-Pierre Dehouck, Masanori Tachikawa, Yutaro Hoshi, Kotaro Omori, Claude-Alain Maurage, Guillaume Strecker, Lucie Dehouck, Marie-Christine Boucau, Yasuo Uchida, Fabien Gosselet, Tetsuya Terasaki, Yannis Karamanos
Summary: This study compared the protein expression levels of human brain-like endothelial cells (hBLECs) and human brain microvessels (hBMVs) using quantitative targeted absolute proteomics. The results showed that hBLECs had higher protein levels of CD144, CD147, MRP4, Annexin A6, and caveolin-1 compared to hBMVs, while the protein levels of MCT1, Connexin 43, TfR1, and claudin-5 were relatively lower. Furthermore, BCRP was found to be more expressed than MDR1 in hBLECs. These findings contribute to a better understanding of the characteristics of hBLECs and demonstrate the equivalence of in vitro BBB models with in vivo models in terms of transport properties.
Review
Biochemistry & Molecular Biology
Maria Abou Diwan, Marwa Lahimer, Veronique Bach, Fabien Gosselet, Hafida Khorsi-Cauet, Pietra Candela
Summary: Accumulating evidence suggests that chronic exposure to low levels of pesticides in the diet affects the gut-microbiota-blood-brain barrier axis. This axis describes the connection between the microbiota, intestinal barrier, and blood-brain barrier. Preclinical observations have shown that pesticides cause gut microbiota alteration, or dysbiosis, which is associated with gastrointestinal disorders and diseases affecting distant organs like the blood-brain barrier. However, the interaction between pesticides, microbiota, intestinal barrier, and blood-brain barrier is not yet fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shiraz Dib, Rodrigo Azevedo Loiola, Emmanuel Sevin, Julien Saint-Pol, Fumitaka Shimizu, Takashi Kanda, Jens Pahnke, Fabien Gosselet
Summary: Neuroinflammation and brain lipid imbalances are observed in individuals with Alzheimer's disease. The tumor necrosis factor-a (TNFa) and liver X receptor (LXR) signaling pathways are involved in both processes, but their relationships in human brain pericytes (HBP) of the neurovascular unit are not well understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Camille Menaceur, Johan Hachani, Shiraz Dib, Sophie Duban-Deweer, Yannis Karamanos, Fumitaka Shimizu, Takashi Kanda, Fabien Gosselet, Laurence Fenart, Julien Saint-Pol
Summary: This study investigates the proteome differences between brain pericytes (BPs) cultured alone (hBP-solo) and in co-culture with brain microvessel endothelial cells (ECs) (hBP-coc) to model the human blood-brain barrier (BBB) in vitro. The results show that hBP-solo has 51 proteins related to cell proliferation, contractility, adhesion, and extracellular matrix elements, indicating an immature cell state. On the other hand, hBP-coc has 90 proteins associated with reduced contractile activities and enhanced metabolic functions, particularly in mitochondrial activities and sterol metabolism, suggesting mature pericytes. This study highlights the role of ECs in promoting the maturation and metabolic switch of BPs during barriergenesis.
Article
Pharmacology & Pharmacy
Erik Melander, Camilla Eriksson, Sara Wellens, Kimia Hosseini, Robert Fredriksson, Fabien Gosselet, Maxime Culot, Ulf Goransson, Margareta Hammarlund-Udenaes, Irena Loryan
Summary: The blood-brain barrier poses challenges to drug delivery to the CNS. CD4, γδT cells, mononuclear phagocytes, mononuclear phagocytes-like antigen-presenting cells, NK cells, NKT cells, resident macrophage-like cells, and neutrophils in the CNS are mainly involved in the innate immune response, while B cells, macrophage-like cells, and T cells play a key role in the acquired immune response.The above findings suggest that cCPPs can serve as potential CNS drug delivery scaffolds, and the differences in their transport across the BBB and cellular uptake abilities are important factors to consider in the development of peptide-based drug delivery systems.
Review
Pharmacology & Pharmacy
Solene Masloh, Maxime Culot, Fabien Gosselet, Anne Chevrel, Leonardo Scapozza, Magali Zeisser Labouebe
Summary: Recombinant biological molecules, due to progress in biotechnology and understanding of subcellular processes, have become cutting-edge in biomedical research. They are increasingly used as drugs for various diseases. However, their limited bioavailability when orally delivered has led to the development of accurate models to determine their capacity to cross the intestinal mucosa. Additionally, strategies to enhance their intestinal permeability and stability have been explored.
Article
Medicine, Research & Experimental
Raphael Leblanc, Rania Ghossoub, Armelle Goubard, Remy Castellano, Joanna Fares, Luc Camoin, Stephane Audebert, Marielle Balzano, Berna Bou-Tayeh, Cyril Fauriat, Norbert Vey, Sylvain Garciaz, Jean-Paul Borg, Yves Collette, Michel Aurrand-Lions, Guido David, Pascale Zimmermann
Summary: This study reveals a vicious signaling loop potentially at the heart of AML-stroma crosstalk and unexpected tumor-suppressive effects of syntenin that need to be considered during systemic targeting of syntenin in cancer therapy.
EMBO MOLECULAR MEDICINE
(2023)
Article
Food Science & Technology
Nathalie Atallah, Claire Gaudichon, Audrey Boulier, Alain Baniel, Benoit Cudennec, Barbara Deracinois, Rozenn Ravallec, Christophe Flahaut, Dalila Azzout-Marniche, Nadezda Khodorova, Martin Chapelais, Juliane Calvez
Summary: The aim of this study was to analyze the protein digestibility and postprandial metabolism in rats of milk protein matrices obtained by different industrial processes. The results showed that ingestion of whey proteins led to higher protein content in the intestinal mucosa and liver, possibly due to fast assimilation. Additionally, industrial processes that modify the structure and/or composition of milk proteins influence protein digestion and utilization.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: Human hemoglobin and bovine hemoglobin both contain rich bioactive peptides, with high similarity between the two. Bioinformatics tools and enzymatic hydrolysis experiments revealed the presence of various bioactive peptides, including some that have never been found in human hemoglobin before.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ahlam Outman, Barbara Deracinois, Christophe Flahaut, Mira Abou Diab, Jihen Dhaouefi, Bernard Gressier, Bruno Eto, Naima Nedjar
Summary: This study focuses on the enzymatic hydrolysis of hemoglobin and compares the antibacterial and antioxidant potentials of human and bovine hemoglobin hydrolysates. The results show strong antimicrobial and antioxidant activities of the peptide hydrolysates, independent of the initial substrate concentration level. The presence of bioactive peptides in human hemoglobin suggests its potential as a valuable source for the food or pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Mouna Ambli, Barbara Deracinois, Anne-Sophie Jenequin, Rozenn Ravallec, Benoit Cudennec, Christophe Flahaut
Summary: Bioinformatics software is essential in peptidomics/proteomics research, enabling the identification of peptides through the comparison of peptide fragmentation spectra obtained by mass spectrometry with targeted databases or by de novo sequencing. Customized software programming is crucial in improving peptide and post-translational modification (PTM) identification rates in the agrofood field.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
