Article
Biochemistry & Molecular Biology
Juan Carlos Vera-Urbina, Susana Selles-Marchart, Ascension Martinez-Marquez, Maria Jose Martinez-Esteso, Maria Angeles Pedreno, Jaime Morante-Carriel, Roque Bru-Martinez
Summary: This study presents the characterization and optimization of t-R production in grape cell cultures. Factors such as growth phase, elicitor concentration, biomass, order of addition, and cell conditions were investigated. The optimal conditions for t-R production were determined, and the results can guide the upscaling of the bioproduction system.
Article
Food Science & Technology
Concepcion Medrano-Padial, Maria Puerto, Tristan Richard, Emma Cantos-Villar, Silvia Pichardo
Summary: The study evaluated the antioxidant effects of grapevine-shoot extract and its major compounds on ROS and GSH levels. ST-99 showed the most promise in reducing ROS levels and increasing GSH content at non-cytotoxic concentrations, indicating its potential as an effective alternative to sulfur dioxide in winemaking.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Horticulture
Fei Ding, Chuang Wang, Shuoxin Zhang, Meiling Wang
Summary: This study reveals the critical role of a JA-responsive glutathione S-transferase gene (SlGSTU24) in alleviating cold stress in tomato. SlGSTU24 is expressed in all tissues and is responsive to both JA and cold stress. Upregulation of SlGSTU24 attenuates cold-induced oxidative stress, while its expression is dependent on the crucial regulator of JA signaling pathway, SlMYC2. These findings uncover a crucial module MYC2-SlGSTU24 that maintains ROS homeostasis in tomato under cold conditions.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study demonstrates that plant endophytes play a significant role in stimulating stilbene production in grape cell suspension, with endophytic fungi showing a more pronounced induction effect on stilbene accumulation compared to endophytic bacteria. The increase in stilbene content was due to the activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. The findings suggest that grape endophytes could potentially serve as stimulators for stilbene biosynthesis in grapevine or grape cell cultures.
Article
Biochemistry & Molecular Biology
Katarzyna Otulak-Koziel, Edmund Koziel, Edit Horvath, Jolan Csiszar
Summary: This study reveals the importance of the redox state of glutathione in the response of plants to virus inoculation. The transcriptionally regulated GSTU19 and GSTU24 play roles in the susceptible and resistance-like reaction of plants against TuMV, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Z. Ogneva, O. A. Aleynova, A. R. Suprun, Y. A. Karetin, A. S. Dubrovina, K. Kiselev
Summary: This study demonstrated that overexpression of VaSTS1 and VaSTS7 genes significantly increased plant tolerance to UV-B and UV-C in A. thaliana plants, while not affecting tolerance to other abiotic stresses. Overexpression of VaSTS1 gene led to the highest levels of trans-piceid and trans-resveratrol in transgenic plants.
BIOLOGIA PLANTARUM
(2021)
Article
Entomology
Siti Nasuha Hamzah, Silas Wintuma Avicor, Zazali Alias, Sarah Abdul Razak, Siti Khadijah Mohd Bakhori, Ting Chuan Hsieh, Nurin Nazifa Syanizam, Salinah Abdul Farouk
Summary: This study aimed to identify the GSTs induced by acute insecticide challenges and analyze the detoxification mechanisms in Aedes albopictus larvae. The findings suggest that Delta and Epsilon GSTs are mainly involved in the detoxification mechanism via direct glutathione metabolism or the sequestration of insecticides, providing protection against oxidative stress induced by insecticide exposure.
Article
Plant Sciences
Changyue Jiang, Dan Wang, Jie Zhang, Yan Xu, Chaohong Zhang, Jianxia Zhang, Xiping Wang, Yuejin Wang
Summary: The transcription factor VqMYB154 from Vitis quinquangularis plays a crucial role in regulating the accumulation of stilbenes, such as resveratrol, in grapevine, thereby enhancing disease resistance. Its pathogen-induced promoter allows it to activate stilbene synthase genes and improve grapevine resistance to pathogens like P. syringae.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Wandi Liu, Chaohui Yan, Ruimin Li, Guanyu Chen, Xinqi Wang, Yingqiang Wen, Chaohong Zhang, Xiping Wang, Yan Xu, Yuejin Wang
Summary: The discovery of the Chinese wild grape gene VqNSTS3 can be used to breed powdery mildew-resistant grapes. VqNSTS3 expression is regulated by VqWRKY33 and enhances resistance to pathogen invasion through enhanced signal transduction and increased VqNSTS3 expression.
HORTICULTURE RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Purificacion Corchete, Lorena Almagro, Jose Antonio Gabaldon, Maria Angeles Pedreno, Javier Palazon
Summary: This study investigates the use of reusable CD polymers coated with magnetic Fe3O4 nanoparticles to enhance the production of bioactive compounds in transgenic cell cultures. The results show that HPCD-EPI-MN treatment significantly increases the accumulation of target products, but high concentrations and prolonged treatment have adverse effects on cell growth and viability. The polymer exhibits good stability and reusability within three elicitation cycles.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tao Zhang, Li Liu, Yulian Jia, Junrui Zhi, Wenbo Yue, Dingyin Li, Guang Zeng
Summary: This study investigated the effect of glutathione S-transferase (GST) gene knockdown on the adaptive ability of western flower thrips to plant defenses. The results showed that RNAi downregulation of FoGSTd1 and FoGSTs1 reduced the anti-adaptive ability of western flower thrips to plant defenses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesca Marzorati, Rossana Rossi, Letizia Bernardo, Pierluigi Mauri, Dario Di Silvestre, Emmanuelle Lauber, Laurent D. Noel, Irene Murgia, Piero Morandini
Summary: This study investigates the early leaf responses of Arabidopsis thaliana to Pseudomonas simiae WCS417 exposure and the potential involvement of formate dehydrogenase (FDH) in these responses. The results reveal that chloroplasts, particularly components of the photosystems PSI and PSII, as well as members of the glutathione S-transferase family, are among the early targets of the metabolic changes induced by WCS417.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Plant Sciences
Pedro Joaquin Sanchez-Pujante, Matteo Gionfriddo, Ana Belen Sabater-Jara, Lorena Almagro, Maria Angeles Pedreno, Pedro Diaz-Vivancos
JOURNAL OF PLANT PHYSIOLOGY
(2020)
Article
Plant Sciences
Victoria Florencio-Ortiz, Susana Selles-Marchart, Jose L. Casas
Summary: Aphid attacks trigger plant defense responses and lead to changes in the proteome of pepper leaves, with low density infestation resulting in a smaller number of differentially expressed proteins compared to high density infestation. The altered proteins are involved in various aspects of plant primary metabolism, with photosynthesis being the most affected process.
Article
Environmental Sciences
A. Gil-Izquierdo, M. A. Pedreno, S. Montoro-Garcia, M. Tarraga-Martinez, P. Iglesias, F. Ferreres, D. Barcelo, E. Nunez-Delicado, J. A. Gabaldon
Summary: This study evaluates the removal capacity of four autochthonous microalgae consortia in wastewater and finds that consortium 1 is the most effective in removing emerging contaminants and nitrate and phosphate.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Daniela P. Herrera, Andrea M. Chanique, Ascension Martinez-Marquez, Roque Bru-Martinez, Robert Kourist, Loreto P. Parra, Andreas Schuller
Summary: This study reports the engineering of a resveratrol OMT from Vitis vinifera with high catalytic efficiency in producing pterostilbene. Through point mutations and variant design, the native substrate selectivity of VvROMT was successfully altered, indicating its potential for the tailor-made production of stilbenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Javier Santamaria-Gomez, Miguel Angel Rubio, Rocio Lopez-Igual, Ana B. Romero-Losada, Fernando M. Delgado-Chaves, Roque Bru-Martinez, Francisco J. Romero-Campero, Antonia Herrero, Michael Ibba, Jesus A. G. Ochoa de Alda, Ignacio Luque
Summary: This study reveals the existence of long tRNA gene arrays in some bacteria, which are induced when the translation machinery is impaired, directly contributing to cell survival. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Philippe Jeandet, Eduardo Sobarzo-Sanchez, Md. Sahab Uddin, Roque Bru, Christophe Clement, Cedric Jacquard, Seyed Fazel Nabavi, Maryam Khayatkashani, Gaber El-Saber Batiha, Haroon Khan, Iwona Morkunas, Franscesco Trotta, Adrian Matencio, Seyed Mohammad Nabavi
Summary: Stilbenes, such as resveratrol and its derivatives, have interesting properties but limited by their poor aqueous solubility and low bioavailability. Cyclodextrins play a significant role in stilbene chemistry, forming nanomolecular inclusion complexes for drug delivery and synthesis of derivatives. Plant cell systems offer a feasible way for large-scale production of stilbenes, requiring elicitor compounds like cyclodextrins for biosynthesis induction.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata Pietrowska-Borek, Jedrzej Dobrogojski, Anna Maria Wojdyla-Mamon, Joanna Romanowska, Justyna Golebiewska, Slawomir Borek, Koichi Murata, Atsushi Ishihara, Maria Angeles Pedreno, Andrzej Guranowski
Summary: The research demonstrates that specific nucleoside 5'-phosphoramidates can enhance the expression of genes related to the phenylpropanoid pathway in plant cells, leading to the accumulation of related products and providing stress resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elias Hurtado-Gaitan, Susana Selles-Marchart, James Hartwell, Maria Jose Martinez-Esteso, Roque Bru-Martinez
Summary: The study found that under CD induction conditions, PPCK was down-regulated, stilbene production increased, and PPC activity was lost in both cell cultures and leaves. Additionally, STS transcripts were co-induced with MYB14 and WRKY24 in both tissues. These genes had not been previously reported to respond to CD in grape leaves.
Article
Biochemistry & Molecular Biology
Lorena Almagro, Antonio A. Calderon, Maria A. Pedreno, Maria A. Ferrer
Summary: Salt treatments significantly alter the production of bioactive phenolic compounds in grapevine suspension cultured cells. Cellular redox homeostasis is maintained by increasing the accumulation of phenolic compounds. The antioxidative network shows a differential response to the presence of elicitors under saline conditions.
Article
Plant Sciences
Karina Andrea Crisostomo-Ayala, Ana Belen Sabater-Jara, Claudia Perez Manriquez, Federico Ferreres, Angel Gil-Izquierdo, Maria Angeles Pedreno, Martha Hernandez de la Torre, Manuel Sanchez-Olate, Darcy Graciela Rios Leal
Summary: This study identified various bioactive compounds in adult maqui leaves and compared them with leaves from in vitro-grown maqui plants. The results showed that the in vitro samples had higher total phenolic content, while winter basal leaves had higher total flavonoid content. The leaves of maqui plants could be a valuable source of antioxidants and lipophilic compounds for various industries.
Article
Plant Sciences
Ana Belen Sabater-Jara, Maria Jesus Marin-Marin, Lorena Almagro, Maria Angeles Pedreno
Summary: In this study, the effect of different elicitors and their combined treatments on triterpene compound production and the expression of key biosynthetic genes in Solanum lycopersicum cv. Micro-Tom suspension-cultured cells were analyzed. The results showed that β-cyclodextrins (CD) act as true elicitors of triterpene biosynthesis and can promote the release of bioactive compounds from tomato cells into the extracellular medium.
Article
Plant Sciences
Ana Belen Sabater-Jara, Lorena Almagro, Isabel Nicolas Sanchez, Maria Angeles Pedreno
Summary: Morus alba L., commonly known as white mulberry, is a plant widely used in Asian traditional medicine for therapeutic purposes. Its extracts have shown efficacy against lipidemia, diabetes, obesity, and possess hepatoprotective and tyrosinase-inhibitory properties. Stilbenes, the bioactive compounds found in M. alba, are gaining attention for their potential activity against COVID-19. This study investigated the effect of elicitation on plant growth and stilbene accumulation, specifically oxyresveratrol and trans-resveratrol, in in vitro cultured M. alba plants. The results showed that elicitation with cyclodextrins and methyl jasmonate significantly increased the total phenolic content and antioxidant capacity of the plants, making them a promising source of bioactive stilbenes for pharmaceutical and cosmeceutical industries.
Article
Biochemistry & Molecular Biology
Juan Carlos Vera-Urbina, Susana Selles-Marchart, Ascension Martinez-Marquez, Maria Jose Martinez-Esteso, Maria Angeles Pedreno, Jaime Morante-Carriel, Roque Bru-Martinez
Summary: This study presents the characterization and optimization of t-R production in grape cell cultures. Factors such as growth phase, elicitor concentration, biomass, order of addition, and cell conditions were investigated. The optimal conditions for t-R production were determined, and the results can guide the upscaling of the bioproduction system.
Review
Biochemistry & Molecular Biology
Philippe Jeandet, Alessandro Vannozzi, Eduardo Sobarzo-Sanchez, Md. Sahab Uddin, Roque Bru, Ascension Martinez-Marquez, Christophe Clement, Sylvain Cordelier, Azadeh Manayi, Seyed Fazel Nabavi, Mahsa Rasekhian, Gaber El-Saber Batiha, Haroon Khan, Iwona Morkunas, Tarun Belwal, Jingjie Jiang, Mattheos Koffas, Seyed Mohammad Nabavi
Summary: Phytostilbenes, despite being a limited chemical family, represent a significant group of molecules among natural compounds. Research interest in these compounds has continuously grown, leading to a vast literature development and tens of thousands of references. The various aspects of phytostilbenes, including their chemistry, biosynthesis regulation, biological activities, molecular engineering, and biotechnological production, have been extensively covered in over 500 articles.
NATURAL PRODUCT REPORTS
(2021)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)