期刊
JOURNAL OF PROTEOMICS
卷 74, 期 8, 页码 1266-1278出版社
ELSEVIER
DOI: 10.1016/j.jprot.2011.02.003
关键词
Cork; Phellem formation; Suberisation; Proteomics; Quercus suber stem
资金
- FCT [POCTI/AGR/39011/2001]
- Fundação para a Ciência e a Tecnologia [POCTI/AGR/39011/2001] Funding Source: FCT
Cork (phellem) formation in Quercus suber stem was studied by proteomic analysis of young shoots of increasing age (Y0, Y1 and Y4) and recently-formed phellem (Y8Ph) and xylem (Y8X) from an 8-year-old branch. In this study 99 proteins were identified, 45 excised from Y8X and 54 from Y8Ph. These ones, specifically associated with phellem, are of carbohydrate metabolism (28%), defence (22%), protein folding, stability and degradation (19%), regulation/signalling (11%), secondary metabolism (9%), energy metabolism (6%), and membrane transport (2%). The identification in phellem of galactosidases, xylosidases, apiose/xylose synthase, laccases and diphenol oxidases suggests intense cell wall reorganization, possibly with participation of hemicellulose/pectin biosynthesis and phenol oxidation. The identification of proteasome subunits, heat shock proteins, cyclophylin, subtilisin-like proteases, 14-3-3 proteins, Rab2 protein and enzymes interacting with nucleosides/nucleic acids gives additional evidence for cellular reorganization, involving cellular secretion, protein turnover regulation and active control processes. The high involvement in phellem of defence proteins (thioredoxin-dependent peroxidase, glutathione-S-transferase, SGT1 protein, cystatin, and chitinases) suggests a strong need for cell protection from the intense stressful events occurring in active phellem, namely, desiccation, pests/disease protection, detoxification and cell death. Identically, highly enhanced defence functions were previously reported for potato periderm formation. (C) 2011 Elsevier B.V. All rights reserved.
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