期刊
JOURNAL OF PROTEOMICS
卷 73, 期 12, 页码 2365-2369出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2010.07.009
关键词
Surface antigens; Surfome; Vaccine; Gel-free proteomics; Gram-positive; SS2
资金
- Genoma Espana
- Spanish Ministry of Education
- Spanish Ministry of Science and Innovation [SAF2008-00733]
- Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia) [P09-CTS-4616]
- Junta de Andalucia [BIO-216]
Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection. (C) 2010 Elsevier B.V. All rights reserved.
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