Article
Chemistry, Analytical
C. Venchiarutti, G. Stadelmann, R. Middendorp, Z. Macsik, A. Venzinb
Summary: In this study, picogram amounts of uranium in particle form were quantified using optical microscopy combined with Isotope Dilution Thermal Ionisation Mass Spectrometry (ID-TIMS). The uranium amount was verified using Isotope Dilution Mufti Collection Inductively Coupled Plasma Mass Spectrometry (ID-MC-ICPMS), and both techniques showed good agreement within uncertainties. The uranium particle material in this study was eventually certified for the uranium isotopic composition of particles and the uranium amount per particle, proving the suitability of the techniques for quantifying picogram amounts of uranium in particulate reference materials.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Huu-Hien Huynh, Amandine Boeuf, Maxence Derbez-Morin, Anne-Marie Dupuy, Beatrice Lalere, Vincent Delatour, Joelle Vinh
Summary: An optimized method for quantifying PCT in human serum at sub-microgram per liter level was developed using LC-MS/MS, with linearity established through peptide-based calibration curves and accuracy evaluated using quality control materials. A correction factor was applied to compensate for digestion incompleteness and material loss, enabling accurate quantification with results traceable to SI units. The method showed a twofold lower PCT concentration compared to immunoassay results when applied to serum samples from sepsis patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Raymond Neil Dalton, Timothy Scott Isbell, Ryan Ferguson, Louis Fiore, Andrei Malic, Jeffrey Anton DuBois
Summary: Early detection of CKD using point of care creatinine and eGFR testing improves patient management outcomes. A study was conducted to evaluate the use of a whole blood creatinine/eGFR device in a rural Nicaraguan population. Results showed a good correlation between all creatinine methods and the gold standard ID-LCMS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Jorge Pitarch-Motellon, Lubertus Bijlsma, Juan Vicente Sancho Llopis, Antoni F. Roig-Navarro
Summary: The study showed that using Isotope Pattern Deconvolution (IPD) quantification method for determining substances in wastewater can reduce uncertainty in estimating illicit drug concentration, improving accuracy and reliability. Additionally, IPD is faster and cost-effective when dealing with high matrix effects, analyzing a large number of samples and compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jianyi Liu, Wen Zhu, Haofeng Sun, Dewei Song, Peng Xiao, Bei Xu, Hongmei Li
Summary: The development of a certified reference material (CRM) for natural CRP, named GBW09228, was reported, with certification results traceable to SI units. This CRM can be used to calibrate and verify the accuracy of CRP measurements, providing a foundation for establishing a complete traceability chain for CRP.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Esther S. Breuninger, Julie Tolu, Sylvain Bouchet, Lenny H. E. Winkel
Summary: Although oceans play a crucial role in the global selenium cycle, there is limited quantitative information available on selenium concentrations and speciation in marine environments. This study developed a sensitive method using species-specific isotope dilution gas chromatography coupled to inductively coupled plasma mass spectrometry (ID-GC-ICPMS) to determine the different selenium chemical fractions in seawater, allowing for the measurement of selenium concentrations in brackish and marine systems.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Marco Maspero, Ettore Gilardoni, Chiara Bonfanti, Graziella Messina, Luca Regazzoni, Marco De Amici, Marina Carini, Giancarlo Aldini, Clelia Dallanoce
Summary: The physiological properties of L-carnosine make it suitable for nutritional ergogenic applications and therapeutic potential. The use of HPLC-MS procedures with isotope-labeled internal standards is the state-of-the-art approach for quantitation in biological matrices. A convenient strategy for the synthesis of 13C-labeled carnosine analogs as internal standards is presented in this study.
Article
Chemistry, Applied
Jingjing Yan, Min Wang, Mengrui Yang, Jian Zhou, Xiaofeng Xue, Tongtong Wang
Summary: This study presents a method for assessing the purity of bovine lactoferrin using mass balance method and amino acid-based isotope dilution mass spectrometry. The results obtained from the two methods showed good consistency, indicating the reliability of the analysis. These analytical methods can be applied in protein quantification and the development of certified reference materials for lactoferrin.
Article
Chemistry, Analytical
Viktoria Lindemann, Jessica Schmidt, Benedikt Cramer, Hans-Ulrich Humpf
Summary: The analysis of trace contaminants in environmental samples is crucial for exposure assessment and evaluation of potential risks to human health. Currently, high resolution mass spectrometry methods are gaining popularity due to their enhanced selectivity, although the complex composition of environmental samples can have significant effects on the performance of mass analyzers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Sueleyman Bodur, Sezin Erarpat, Omer Tahir Gunkara, Sezgin Bakirdere
Summary: An accurate, sensitive, and rapid analysis methodology for the determination of fenuron at ultratrace levels in water and fruit juice samples was developed based on quadruple isotope dilution (ID4) and liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) system. The developed method showed high accuracy, precision, and sensitivity for the quantification of fenuron at trace levels in the selected samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Rossella Barone, Matteo Conti, Pier Giorgio Cojutti, Milo Gatti, Pierluigi Viale, Federico Pea
Summary: Dalbavancin (DBV) is a long-acting antistaphylococcal lypoglycopeptide increasingly used for long-term treatment of subacute and/or chronic infections. Population pharmacokinetic studies have shown that two 1500mg doses of DBV 1 week apart can effectively treat infections for weeks. This study developed and validated a fast and simple analytical method for measuring DBV concentrations in human plasma microsamples using Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry (ITD LC-MS/MS) technique.
Article
Biochemical Research Methods
Garnet McRae, Donald M. Leek, Juris Meija, Bryn Shurmer, Steven J. Lehotay, Joachim Polzer, Jeremy E. Melanson, Zoltan Mester
Summary: A freeze-dried bovine muscle-certified reference material (CRM), called BOTS-1, containing residues of commonly used veterinary drugs, was produced and certified. Liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were used for value assignment, along with isotope dilution and standard addition approaches. Data from multiple organizations were used for value assignment. Results were obtained through an international inter-laboratory comparison. Primary standards were characterized using quantitative NMR (1H-qNMR). The certified mass fractions of the veterinary drug residues were reported with expanded uncertainties.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Maite Aramendia, Diego Leite, Javier Resano, Martin Resano, Kharmen Billimoria, Heidi Goenaga-Infante
Summary: This paper presents a methodology based on isotope dilution for the mass determination and sizing of silver nanoparticles using single-particle ICP-TOFMS. The proposed method measures NP isotope ratios in a particle-to-particle approach, allowing for the characterization of NP mass and size distributions. The method optimization for precision and accuracy included the selection of spike isotopic composition and concentration.
Article
Agriculture, Multidisciplinary
Eva Hoelzle, Laura Becker, Claudia Oellig, Michael Granvogl
Summary: Considerable amounts of acrylamide were detected in black table olives. Heat treatment did not affect the amount of acrylamide in black table olives, but significantly increased the levels in green and naturally black table olives. The content of free asparagine remained unchanged before and after heat treatment, while the amount of 3-aminopropionamide increased due to heat impact.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Eva Hoelzle, Laura Becker, Claudia Oellig, Michael Granvogl
Summary: This study investigated the levels of acrylamide, free asparagine, and 3-aminopropionamide in black, green, and naturally black table olives before and after heat treatment. The results showed that the acrylamide content in black table olives was high and remained unchanged after heat treatment, while green and naturally black table olives had low acrylamide content before heat treatment but significantly increased after heating.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Fernanda Batistel, Abdulrahman S. Alharthi, Rainie R. C. Yambao, Ahmed A. Elolimy, Yuan-Xiang Pan, Claudia Parys, Juan J. Loor
JOURNAL OF NUTRITION
(2019)
Article
Endocrinology & Metabolism
Russell Emmons, Guanying Xu, Diego Hernandez-Saavedra, Adam Kriska, Yuan-Xiang Pan, Hong Chen, Michael De Lisio
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2019)
Article
Biochemistry & Molecular Biology
Laura Moody, Guanying Bianca Xu, Hong Chen, Yuan-Xiang Pan
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2019)
Article
Biochemistry & Molecular Biology
Laura Moody, Daniel Kougias, Paul M. Jung, Isabel Digan, Aaron Hong, Aleksandra Gorski, Hong Chen, Janice Juraska, Yuan-Xiang Pan
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2019)
Review
Nutrition & Dietetics
Diego Hernandez-Saavedra, Laura Moody, Guanying Bianca Xu, Hong Chen, Yuan-Xiang Pan
ADVANCES IN NUTRITION
(2019)
Review
Oncology
Laura Moody, Svyatoslav Dvoretskiy, Ruopeng An, Suparna Mantha, Yuan-Xiang Pan
Article
Endocrinology & Metabolism
Huan Wang, Guanying Bianca Xu, Diego Hernandez-Saavedra, Hong Chen, Yuan-Xiang Pan
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2020)
Article
Physiology
Tiffany Y. Yang, Jennie C. Gardner, Zijun Gao, Yuan-Xiang Pan, Nu-Chu Liang
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2020)
Article
Gastroenterology & Hepatology
Huan Wang, Guanying Bianca Xu, Hong Chen, Yuan-Xiang Pan
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2020)
Article
Nutrition & Dietetics
Mingzhu Cai, Jie Zhang, Hong Chen, Yuan-Xiang Pan
Summary: This study reveals that maternal protein restriction can transcriptionally program hepatic autophagy-related gene expression specifically in female rat offspring through the activation of the eIF2 alpha/ATF4 pathway and intricate regulation by transcription factors such as ATF4 and CHOP.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Biochemical Research Methods
Laura Moody, Guanying Bianca Xu, Yuan-Xiang Pan, Hong Chen
Summary: This study utilizes a novel mixture modeling approach to identify bimodal miRNA in cancer and demonstrates their importance in predicting overall survival and drug response. The results reveal that high levels of bimodal miRNA expression are characteristic of cancer, and certain miRNA consistently account for tumor heterogeneity and may be involved in oncogenic processes. Bimodal miRNA can be used as biomarkers for prognosis and personalized treatment strategies in certain types of cancer.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guanying Bianca Xu, Mingzhu Cai, Fatma Zehra Kadayifci, Juncen Dong, Shasha Zheng, Wenyan Mei, Wen Zhao, Yuan-Xiang Pan, Hong Chen
Summary: This study found that a diet with increased nutrient density relieved the inflammation induced by knockout of Hnrnp I, partially due to the reduced expression of inflammatory and immune-modulating cytokines in the mouse distal colon.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Guanying (Bianca) Xu, Yuan-Xiang Pan, Wenyan Mei, Hong Chen
Summary: This study reveals the critical cellular function of colonic tuft cells in promoting type-2 immune responses by communicating with ILC2s via the L1CAM-integrins interaction. This communication leads to increased secretion of inflammatory cytokines in ILC2s.
Article
Agriculture, Dairy & Animal Science
Qianming Jiang, Danielle N. Sherlock, Huimin Zhang, Jessie Guyader, Yuan-Xiang Pan, Juan J. Loor
Summary: Physiological and environmental stresses can lead to gastrointestinal tract dysfunction. One-carbon metabolism contributes to anti-oxidant capacity and the synthesis of various compounds. A study on dairy cows revealed unique differences in one-carbon metabolism and related pathways across sections of the gastrointestinal tract. The findings suggest that targeting these pathways during stressful periods is important.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Nutrition & Dietetics
Mario Vailati-Riboni, Fernanda Batistel, Rainie R. C. S. Yambao, Claudia Parys, Yuan-Xiang Pan, Juan J. Loor
CURRENT DEVELOPMENTS IN NUTRITION
(2019)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)