Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
Article
Plant Sciences
Cheng Song, Yunpeng Zhang, Muhammad Aamir Manzoor, Guohui Li
Summary: Jasmonate signaling is important for plant stress responses and secondary metabolism. This study established a rapid identification method for alkaloids in Dendrobium officinale and identified seven alkaloids and forty-nine other chemical substances in D. officinale. These findings provide insights into the composition of alkaloids in Dendrobium plants and offer a scientific method for their identification.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Peng Zhao, Jiangbing Qiu, Aifeng Li, Guowang Yan, Min Li, Ying Ji
Summary: This study found that the extraction ratio has a significant impact on the accuracy of BMAA analyzed in different biological samples, recommending the best extraction ratios for different matrices. The comparison enhances the performance of solid-phase extraction (SPE) purification protocol and the accuracy of LC-MS/MS analysis of BMAA.
Article
Biochemical Research Methods
Pingping Wang, Fangling Wu, Fuxing Xu, Jun Jack Hu, Chuan-Fan Ding
Summary: The study developed a special poly solid-phase extraction (in-tube SPE) column for extraction analysis combined with UPLC, demonstrating excellent extraction efficiency, good precision, and satisfactory sensitivity for preservatives detection. The method is characterized by simple sample pretreatment, high throughput, rapid analysis, cost-effectiveness, and satisfactory sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dennis Kroesser, Benjamin Dreyer, Bente Siebels, Hannah Voss, Christoph Krisp, Hartmut Schlueter
Summary: This study analyzed five different truffle species on the proteome level, obtaining unique proteomic fingerprints for each species which can be used to detect food fraud. Further exploration of the identified proteins may lead to deeper understanding of truffle biochemistry in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Francisco Calderon Celis, Ivan Gonzalez-Alvarez, Magdalena Fabjanowicz, Simon Godin, Laurent Ouerdane, Beatrice Lauga, Ryszard Lobinski
Summary: Metallophores play a crucial role in metal scavenging and acquisition for many organisms. However, their identification in highly organic matter rich environments like peatlands is challenging due to low solubilization and metal scarcity. In this study, a novel two-dimensional solid-phase extraction-liquid chromatography-mass spectrometry (SPE-LC-MS) approach was developed for sensitive detection and identification of metallophores in native environments. By applying this method, 53 metallophores, including 36 bacterial ones, were identified in peat collected from a French peatland. The detection of characteristic metal isotope patterns also revealed potential complexes of iron and copper. Furthermore, a taxonomic-based inference method was implemented to associate the identified bacterial metallophores with potential producers, suggesting a new way to explore poorly documented environments for new metallophores and their producers.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Lorenzo Martello, Nina Maria Ainali, Dimitrios N. Bikiaris, Dimitra A. Lambropoulou
Summary: In this study, novel molecularly imprinted polymers (MIPs) were synthesized using penconazole as the template and various combinations of functional monomers and cross-linkers. The MIPs showed higher affinity for triazole pesticides in apple juice compared to non-imprinted polymers. The developed MIP-d-SPE-LC-MS method exhibited excellent selectivity and good performance for the enrichment and determination of triazole compounds in apple juice and river water samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Philipp Schmidt, Christine Kolb, Markus Godejohann, Andreas Reiser, Markus Philipp, Hans-Christian Mueller, Konstantin Karaghiosoff
Summary: In this study, we report the use of a hyphenated HPLC-DAD-HRMS/SPE NMR system for the separation and isolation of a complex mixture of esters. By utilizing this system, sixteen highly similar individual esters were successfully isolated and differentiated, demonstrating its applicability in pharmaceutical analysis and drug quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Liju Fan, Jing An, Jin Gao, Yanjun Cui, Zhanjun Dong
Summary: A novel extraction procedure using polystyrene nanofibers as a solid-phase extraction sorbent was developed for collecting atypical antipsychotics from human plasma. The method showed simplicity, selectivity, and high efficiency, making it suitable for simultaneous quantification of seven atypical antipsychotics in human plasma.
Article
Biochemical Research Methods
Ahmed M. M. Abdel-Megied, Isaac A. A. Monreal, Limian Zhao, Alex Apffel, Hector C. C. Aguilar, Jace W. W. Jones
Summary: Emerging and re-emerging zoonotic viral diseases, particularly enveloped viruses like SARS-CoV-2, have a significant impact on public health. The lipid composition of the viral envelope and changes in host cell lipid metabolism during viral infection provide an opportunity to understand how these viruses infect cells. In this study, the lipid composition of VeroE6-TMPRSS2 cells infected with SARS-CoV-2 was characterized, with a focus on the ceramide to sphingomyelin ratio. This lipid profile can help in the development of antiviral strategies and vaccines.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Jiri Palat, Petr Kukucka, Garry P. Codling, Elliott J. Price, Petr Janku, Jana Klanova
Summary: A 96-well plate solid phase extraction method was developed for determination of selected POPs in low volume blood serum, with extraction efficiency tested on certified reference materials and demonstrated on real samples from pregnant women.
Review
Spectroscopy
Debabrata Panda, Bisnu P. Dash, Sivakumar Manickam, Grzegorz Boczkaj
Summary: The accurate identification and quantification of biotoxins pose a critical challenge, and liquid chromatography-mass spectrometry provides an accurate method for addressing this issue. Both LRMS and HRMS methods have been widely implemented for biotoxin detection, and significant progress has been made in sampling device utilization, novel sample preparation strategies, and synthesizing toxin standards.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Chemistry, Applied
Philipp Eyring, Marc Tienstra, Hans Mol, Susan Strange Herrmann, Peter Have Rasmussen, Henrik Lauritz Frandsen, Mette Erecius Poulsen
Summary: Various generic extraction methods have been used for determination of pesticide residues, mycotoxins, and PAHs in food and animal feed, but a new method introduced in this study facilitated identification of a wide range of compounds across different polarities. The method involved initial extraction with water, acetonitrile, formic acid, and heptane followed by solid-phase extraction clean-up and analysis by chromatography and mass spectrometry, achieving analyte recoveries with high accuracy and precision.
Article
Biochemistry & Molecular Biology
Alex Apffel, Limian Zhao, Mark J. Sartain
Summary: This article introduces a novel solid-phase extraction method for extracting lipids from human plasma, which saves time and labor compared to traditional liquid-liquid extraction methods, and provides equivalent or better qualitative and quantitative results in terms of recovery, reproducibility, and lipidome coverage.
Article
Biochemistry & Molecular Biology
Vanessa Andrea Meling, Kjetil Berge, David Lausten Knudsen, Per Ola Ronning, Cato Brede
Summary: The aquaculture industry is a sustainable source of food for humans, but there are still challenges in terms of disease and ethical concerns. This study presents a new method for measuring cortisol in fish feces and finds that the hydrolysis and extraction conditions affect the analysis results. The findings show that cortisol in feces from farmed Atlantic salmon is mainly conjugated to sulfate. The method allows for quantification of cortisol and reveals that fish experience high stress levels after being released into seawater, which significantly reduce after eight days.
Article
Biochemical Research Methods
Soumya Mukherjee, Andris Jankevics, Florian Busch, Markus Lubeck, Yang Zou, Gary Kruppa, Albert J. R. Heck, Richard A. Scheltema, Karli R. Reiding
Summary: Ion mobility enables spatial separation of ions in the gas phase, providing information about their size. The timsTOF Pro device can physically separate N-glycopeptides from nonmodified peptides and produce high-quality fragmentation spectra. This method allows for the effective selection of analytes of interest based on the clear cluster in the mobiologram formed by the glycan moieties enlarging the size of glycopeptides.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemical Research Methods
Wouter van Bergen, Johannes F. Hevler, Wei Wu, Marc P. Baggelaar, Albert J. R. Heck
Summary: Most drugs target proteins, and determining the exact drug binding sites on proteins is crucial for understanding their effects. A strategy called PhosID-ABPP was developed to identify drug binding sites using immobilized metal-affinity chromatography and phosphonate affinity tags. This method successfully identified over 500 unique binding sites of the drug PF-06672131. PhosID-ABPP also revealed differences in binding sites between intact cells and cell lysates, and captured a previously elusive binding site on the epidermal growth factor receptor.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Review
Chemistry, Multidisciplinary
Evolene Desligniere, Amber Rolland, Eduard H. T. M. Ebberink, Victor Yin, Albert J. R. Heck
Summary: Native mass spectrometry is widely used for determining the mass of intact proteins and their biomolecular assemblies. However, it can be challenging for heterogeneous protein complexes. In 2012, an Orbitrap-based mass analyzer with extended mass range was introduced, enabling high-resolution mass spectra of large protein assemblies and single ion measurements. This led to the development of single-molecule Orbitrap-based charge detection mass spectrometry in 2020, which has opened doors for innovative research in various systems.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Gunasekaran Subramaniam, Katharina Schleicher, Duangnapa Kovanich, Anna Zerio, Milda Folkmanaite, Ying-Chi Chao, Nicoletta C. Surdo, Andreas Koschinski, Jianshu Hu, Arjen Scholten, Albert J. R. Heck, Maria Ercu, Anastasiia Sholokh, Kyung Chan Park, Enno Klussmann, Viviana Meraviglia, Milena Bellin, Sara Zanivan, Svenja Hester, Shabaz Mohammed, Manuela Zaccolo
Summary: In this study, previously unrecognized cAMP nanodomains associated with beta-adrenergic stimulation were identified using an integrated phosphoproteomics approach and network analysis. The composition and function of one of these nanodomains were validated. The findings reveal a mechanism that explains the negative long-term clinical outcome observed in patients with heart failure treated with PDE3 inhibitors.
CIRCULATION RESEARCH
(2023)
Article
Medicine, Research & Experimental
Dario A. T. Cramer, Vojtech Franc, Anna-Katharina Heidenreich, Michaela Hook, Mahdi Adibzadeh, Dietmar Reusch, Albert J. R. Heck, Markus Haberger
Summary: This article focuses on the characterization of high molecular weight species (HMWs) in complex antibody formats, such as bispecifics, and their impact on activity. The study presents a multi-method approach for the identification, analysis, and understanding of HMW by-products, which is important for the development and evaluation of therapeutic bispecific antibodies.
Article
Biochemistry & Molecular Biology
Johannes F. Hevler, Pascal Albanese, Alfredo Cabrera-Orefice, Alisa Potter, Andris Jankevics, Jelena Misic, Richard A. Scheltema, Ulrich Brandt, Susanne Arnold, Albert J. R. Heck
Summary: The tricarboxylic acid cycle is a central pathway for energy production in eukaryotic cells and plays a key role in aerobic respiration across all life kingdoms. The 2-oxoglutarate dehydrogenase complex (OGDHC) is a crucial enzyme in this cycle, generating NADH by oxidatively decarboxylating 2-oxoglutarate to succinyl-CoA. We provide evidence that MRPS36 is an important component of eukaryotic OGDHC, supported by cross-linking mass spectrometry data and phylogenetic analyses. We propose that MRPS36 evolved as an E3 adaptor protein, functionally replacing the peripheral subunit-binding domain (PSBD) in eukaryotic E2o.
Article
Andrology
Min Zhang, Riccardo Zenezini Chiozzi, Elizabeth G. Bromfield, Albert J. R. Heck, J. Bernd Helms, Bart M. Gadella
Summary: This study aimed to identify the interacting partners of CRISP2. The interactions of these binding partners were investigated under different conditions. The results suggest that CRISP2 may act as a scaffold for protein complex formation and dissociation to ensure the correct positioning of proteins required for the acrosome reaction and zona pellucida penetration.
Article
Biology
Leire Aguinagalde Salazar, Maurits A. den Boer, Suzanne M. Castenmiller, Seline A. Zwarthoff, Carla de Haas, Piet C. Aerts, Frank J. Beurskens, Janine Schuurman, Albert J. R. Heck, Kok van Kessel, Suzan H. M. Rooijakkers
Summary: In this study, it is found that by modifying the structure of monoclonal antibodies (mAbs), the immune protection and bactericidal effect against Streptococcus pneumoniae can be improved. The modified mAbs effectively activate the complement system and recruit complement component C1 for bacterial clearance, enhancing the antibacterial activity against various serotypes of pneumococci. This study provides an important proof of concept for the future development of antibody therapies against encapsulated bacteria.
Article
Biotechnology & Applied Microbiology
Yen-Hsi Chen, Weihua Tian, Makiko Yasuda, Zilu Ye, Ming Song, Ulla Mandel, Claus Kristensen, Lorenzo Povolo, Andre R. A. Marques, Tomislav Caval, Albert J. R. Heck, Julio Lopes Sampaio, Ludger Johannes, Takahiro Tsukimura, Robert Desnick, Sergey Y. Y. Vakhrushev, Zhang Yang, Henrik Clausen
Summary: Currently available enzyme replacement therapies for lysosomal storage diseases are limited in their effectiveness due to short circulation times and suboptimal biodistribution of the therapeutic enzymes. Researchers have engineered Chinese hamster ovary (CHO) cells to produce glycoengineered enzymes, which have improved circulation time and biodistribution. This glycoengineering approach, known as Long-Acting-GlycoDesign (LAGD), may be widely applicable to lysosomal replacement enzymes to improve their circulatory stability and therapeutic efficacy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Beiping Miao, Zhaoqing Hu, Riccardo Mezzadra, Lotte Hoeijmakers, Astrid Fauster, Shangce Du, Zhi Yang, Melanie Sator-Schmitt, Helena Engel, Xueshen Li, Caroline Broderick, Guangzhi Jin, Raquel Gomez-Eerland, Lisette Rozeman, Xin Lei, Hitoshi Matsuo, Chen Yang, Ingrid Hofland, Dennis Peters, Annegien Broeks, Elke Laport, Annika Fitz, Xiyue Zhao, Mohamed A. A. Mahmoud, Xiujian Ma, Sandrine Sander, Hai-kun Liu, Guoliang Cui, Yu Gan, Wei Wu, Yanling Xiao, Albert J. R. Heck, Wenxian Guan, Scott W. Lowe, Hugo M. Horlings, Cun Wang, Thijn R. Brummelkamp, Christian U. Blank, Ton N. M. Schumacher, Chong Sun
Summary: The dysregulation of immune checkpoint molecules allows cancer cells to escape immune destruction. CD58, an important costimulatory ligand, is found to be positively regulated by CMTM6, which also interacts with PD-L1. The presence of CMTM6 and CD58 on tumor cells significantly affects T cell-tumor interactions and the response to PD-L1-PD-1 blockade.
Article
Cell Biology
Dusanka Milenkovic, Jelena Misic, Johannes F. Hevler, Thibaut Molinie, Injae Chung, Ilian Atanassov, Xinping Li, Roberta Filograna, Andrea Mesaros, Arnaud Mourier, Albert J. R. Heck, Judy Hirst, Nils-Goran Larsson
Summary: The mammalian respiratory chain complexes CI, CIII2, and CIV form a stable assembly called the respirasome, which is critical for cellular bioenergetics. By studying knockin mice with decreased levels of respirasomes, researchers found that high levels of respirasomes are dispensable for maintaining bioenergetics and physiology in mice. However, the alternate functions of respirasomes, such as regulating protein stability and preventing age-associated protein aggregation, need further investigation.
Article
Biochemical Research Methods
Johannes F. Hevler, Albert J. R. Heck
Summary: Mitochondria, packed with proteins, play important roles in various cellular processes. While many mitochondrial protein complexes have been identified, some protein-protein interactions remain elusive. Cross-linking mass spectrometry (XL-MS) has proven to be a valuable tool for the in-depth characterization of these interactions. In this article, experimental strategies for the analysis of proteome-wide protein-protein interactions in mitochondria using XL-MS are highlighted, along with recent technological advances that can further enhance the in situ characterization of these interactions.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Oncology
Marjolein C. Stip, Mitchell Evers, Maaike Nederend, Chilam Chan, Karli R. Reiding, Mirjam J. Damen, Albert J. R. Heck, Sofia Koustoulidou, Ruud Ramakers, Gerard C. Krijger, Remmert de Roos, Edouard Souteyrand, Annelisa M. Cornel, Miranda P. Dierselhuis, Marco Jansen, Mark de Boer, Thomas Valerius, Geert van Tetering, Jeanette H. W. Leusen, Friederike Meyer-Wentrup
Summary: Researchers engineered an antibody called IgA3.0 ch14.18, which shows promise as a new therapy for neuroblastoma. The antibody has a longer half-life, increased protein stability, and potent tumor-killing abilities.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Inge Gazi, Karli R. Reiding, Andre Groeneveld, Jan Bastiaans, Thom Huppertz, Albert J. R. Heck
Summary: We monitored the changes in bovine milk IgG over a 28-day period after calving, finding that IgG accounts for over 50% of protein content in colostrum but less than 3% in mature milk. The N-glycosylation profile of bovine milk IgG was found to be highly heterogeneous with over 40 glycoforms, and this profile changed significantly during lactation. We also identified the presence of IgG3 subtype in bovine milk, alongside IgG1 and IgG2. These findings are important for understanding calf's immune development and the nutritional value of bovine milk.
Article
Immunology
Kelly A. Dingess, Max Hoek, Danique M. H. van Rijswijk, Sem Tamara, Maurits A. den Boer, Tim Veth, Mirjam J. A. Damen, Arjan Barendregt, Michelle Romijn, Hannah G. Juncker, Britt J. van Keulen, Gestur Vidarsson, Johannes B. van Goudoever, Albert Bondt, Albert J. R. Heck
Summary: The most abundant immunoglobulin in the human body is IgA and it is found in high concentrations in mucosal lining and biofluids like milk. The structure and clonal repertoire of IgA1-containing molecular assemblies were analyzed using mass spectrometry-based approach in serum and milk from three donors. The results showed that serum IgA1 consists of two distinct structural populations, monomeric IgA1 and dimeric J-chain coupled IgA1, while IgA1 in milk is present only as secretory IgA (SIgA) with various assemblies. The IgA1-Fab repertoires in serum and milk were also found to be different.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
