Article
Environmental Sciences
Steve U. Ayobahan, Julia Alvincz, Hannes Reinwald, Jannis Strompen, Gabriela Salinas, Christoph Schaefers, Elke Eilebrecht, Sebastian Eilebrecht
Summary: Endocrine disruptors (EDs) have long-lasting negative effects on reproduction in humans and the environment, making them unsuitable for approval for marketing. However, regulatory testing for endocrine disruption is time-consuming, expensive, and requires animal testing. This study combines sublethal zebrafish embryo assays with transcriptomics and proteomics to identify predictive biomarkers for sexual endocrine disruption. Evaluation of these biomarkers in zebrafish embryo-based bioassays can help identify endocrine disrupting hazards and reduce animal testing.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Nikola Mitovic, Stefan Maksimovic, Darko Puflovic, Sanjin Kovacevic, Srdjan Lopicic, Jasna Todorovic, Svetolik Spasic, Marko Dincic, Jelena Nesovic Ostojic
Summary: Research shows that exposure to cadmium in early zebrafish development leads to hatching delays and morphological abnormalities, indicating significant effects of toxic metals on embryological processes.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniel Zarski, Aurelie Le Cam, Thomas Frohlich, Miwako Kosters, Christophe Klopp, Joanna Nynca, Slawomir Ciesielski, Beata Sarosiek, Katarzyna Dryl, Jerome Montfort, Jaroslaw Krol, Pascal Fontaine, Andrzej Ciereszko, Julien Bobe
Summary: This study investigates the contribution of maternally-inherited mRNA and proteins to fish egg molecular cargo and developmental competence using pikeperch as a model. The findings reveal distinct functions of transcripts and proteins in fish eggs, with mRNA shaping embryo neurodevelopment and proteins protecting against pathogens. Pre-ovulation processes can significantly impact reproductive success by modifying gene expression crucial for embryonic development, serving as novel markers for fish egg quality. These results are crucial for understanding the impact of external factors on reproductive fitness in fish species.
Article
Biotechnology & Applied Microbiology
Veronika Jedlickova, Vaclav Hejret, Martin Demko, Pavel Jedlicka, Marie Stefkova, Helene S. Robert
Summary: This study investigates the transcriptional response of different rapeseed cultivars to heat stress and reveals the molecular mechanisms underlying the phenotypic changes during early seed development. The results suggest that the response to reactive oxygen species, seed photosynthesis, and hormonal regulation may be critical factors for heat tolerance in oilseed rape.
Article
Engineering, Environmental
Alper James G. Alcaraz, David Potesil, Kamil Mikulasek, Derek Green, Bradley Park, Connor Burbridge, Kerstin Bluhm, Othman Soufan, Taylor Lane, Marek Pipal, Markus Brinkmann, Jianguo Xia, Zbynek Zdrahal, David Schneider, Doug Crump, Niladri Basu, Natacha Hogan, Markus Hecker
Summary: This study aimed to develop a comprehensive early life stage toxicity pathway model for fish exposed to estrogenic chemicals, rooted in mechanistic toxicology. The integration of omics data improved the interpretation of perturbations in early life stage fish, showing promise as a replacement for standard adult live animal tests. The study provided evidence of conservation of toxicity pathways across levels of biological organization.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sarena Banu, Namami Gaur, Sowmya Nair, Tanuja Ravikrishnan, Shahida Khan, Sandhya Mani, Swarna Bharathi, Komal Mandal, Sowmya Vuppaladadium, Rowmika Ravi, Mir Quoseena, Mohammed M. Idris
Summary: This study utilized differential transcriptomics and proteomics analysis to investigate the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue. The findings provide detailed insights into the expression of genes/proteins associated with the epimorphic regeneration process.
Article
Environmental Sciences
Edward J. Perkins, Kimberly T. To, Lindsey St Mary, Charles H. Laber, Anthony J. Bednar, Lisa Truong, Robyn L. Tanguay, Natalia Garcia-Reyero
Summary: Dyes Disperse Blue 14, Disperse Red 9, Solvent Red 169, and Solvent Yellow 33 have been found to adversely affect zebrafish embryos, with Disperse Blue 14 and Solvent Yellow 33 having the most significant impact on development and behavior.
Article
Multidisciplinary Sciences
Lei Li, Lin Huang, Chenyang Huang, Jia Xu, Yukai Huang, Haihua Luo, Xinya Lu, Shuyue He, Gang Yuan, Li Chen, Xue Han, Xusong Cao, Aolin Jiang, Cuiting Liu, Junmin Shi, Hong Yang, Yong Jiang
Summary: This study focused on the molecular dynamics profiles of exosomes in serum during the development of sepsis. High-performance liquid chromatography-tandem mass spectrometry and RNA-seq were used to detect the dynamic profiles of exosome proteins and RNAs. Integrative multiomics analyses revealed the association of exosome components with various biological processes and molecular pathways. Importantly, exosomes derived from septic serum showed potential therapeutic effects in septic mice.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Obstetrics & Gynecology
John D. Aplin, Adam Stevens
Summary: The text discusses the importance of precise and personalized timing of the endometrial cycle for optimal embryo replacement, highlighting the challenges and uncertainties in defining the relationship between the endometrial cycle and implantation. Despite advancements in 'omics technologies, there remains a lack of consensus regarding the molecular characterization of the receptive state, suggesting a need for further research and exploration in this area.
HUMAN REPRODUCTION
(2022)
Article
Medicine, Research & Experimental
Rita Vaz-Rodrigues, Lorena Mazuecos, Margarita Villar, Marinela Contreras, Sara Artigas-Jeronimo, Almudena Gonzalez-Garcia, Christian Gorazar, Jose de la Fuente
Summary: This study used a multi-omics approach to investigate the impact of tick saliva and mammalian meat consumption on the gut transcriptome and proteome of zebrafish. The results revealed significant changes in biological and metabolic pathways associated with the alpha-Gal syndrome. Tick saliva treatment was found to increase zebrafish mortality, allergic reactions, and changes in behavior and feeding patterns. The analysis of transcriptomics and proteomics data provided insights into the pathways involved in allergic reactions, cardiac and vascular function, and nervous and musculoskeletal disorders.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Article
Engineering, Environmental
Jing Fu, Zhiyuan Gong, Sungwoo Bae
Summary: The study found that the presence of triclosan and methyl triclosan in water can have different toxic effects on zebrafish embryos, impacting mitochondrial inner membrane function and leading to dysregulation of various GO terms and KEGG pathways.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Cell Biology
M. Saeid Jami, Hiromi Murata, Lisa M. Barnhill, Sharon Li, Jeff M. Bronstein
Summary: Neurodegenerative diseases are a major cause of disability worldwide, and environmental factors play a significant role in their development. Recent research has found that prolonged exposure to air pollution increases the risk of Alzheimer's and Parkinson's diseases, although the specific molecular mechanisms are not well understood. This study exposed zebrafish embryos to diesel exhaust particle extract (DEPe) and identified several disrupted molecular pathways relevant to neurodegenerative disorders.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Food Science & Technology
Jutty Rajan Prashanth, Sebastien Dutertre, Subash Kumar Rai, Richard J. Lewis
Summary: The compartmentalization of the venom gland in cone snails enabled the repurposing of venom peptides to facilitate the dietary shift from vermivory to molluscivory and piscivory.
Review
Microbiology
Alina Minias, Lidia Zukowska, Ewelina Lechowicz, Filip Gasior, Agnieszka Knast, Sabina Podlewska, Daria Zygala, Jaroslaw Dziadek
Summary: Tuberculosis is a global infectious disease caused by Mycobacterium tuberculosis, leading to significant mortality. The complexity and length of treatment, as well as the emergence of drug-resistant strains, highlight the importance of discovering new antitubercular compounds. Modern -omic technologies play a crucial role in advancing drug discovery process for tuberculosis treatment.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ranjala Ratnayake, Sarath P. Gunasekera, Jia Jia Ma, Long H. Dang, Thomas J. Carney, Valerie J. Paul, Hendrik Luesch
Letter
Dermatology
A. Y. T. Loh, C. M. Ho, S. Muthiah, B. Venkatesh, S. Zwolinski, A. P. J. J. Bray, B. Reversade, N. Rajan, T. J. Carney
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Yohendran Baskaran, Felicia Pei-Ling Tay, Elsa Yuen Wai Ng, Claire Lee Foon Swa, Sheena Wee, Jayantha Gunaratne, Edward Manser
Summary: The study reveals the association of PAK4 with Afadin-dependent cell-cell junctions and identifies potential phosphorylation substrates of PAK4. Within the cellular environment, PAK4 is found to be linked to Afadin and the nectin family of junctional proteins; PAK4 depends on Afadin for its localization at cell-cell junctions.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Jiajia Ma, Claire A. Scott, Ying Na Ho, Harsha Mahabaleshwar, Katherine S. Marsay, Changqing Zhang, Christopher Kj Teow, Ser Sue Ng, Weibin Zhang, Vinay Tergaonkar, Lynda J. Partridge, Sudipto Roy, Enrique Amaya, Tom J. Carney
Summary: This study reveals that mutations in the hai1a gene in zebrafish lead to a series of cellular responses, including inflammation and calcium signaling, without inducing epithelial cell motility. Inhibition of the Gq subunit can rescue these abnormal phenotypes, and increased MAPK pathway activity may be the cause of epidermal defects.
Article
Biochemical Research Methods
Jeeranan Boonruangkan, Hamid Farrokhi, Thazhe M. Rohith, Samuel Kwok, Tom J. Carney, Pei-Chen Su, Young-Jin Kim
Summary: This study successfully measured heart rate and blood flow in zebrafish embryos using FCR-QPI technique, revealing the average velocity of RBCs and heart rate at different stages of development.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Multidisciplinary Sciences
Katherine S. Marsay, Sarah Greaves, Harsha Mahabaleshwar, Charmaine Min Ho, Henry Roehl, Peter N. Monk, Tom J. Carney, Lynda J. Partridge
Summary: Collective cell migration of the posterior lateral line primordium in zebrafish development is modulated by Cd9, which contributes to effective chemokine signaling. Cd9a and Cd9b function partially redundantly in pLLP migration, with their loss leading to delayed migration and reduced neuromast numbers. Loss of both Cd9a and Cd9b sensitizes embryos to reduced levels of Cxcr4b and Cxcl12a.
Article
Cell Biology
Ranran Liu, Nurgul Imangali, Lalith Prabha Ethiraj, Tom James Carney, Christoph Winkler
Summary: In this study, transcriptome profiling of bone cells in a medaka model was used to identify the crucial role of mmp13b gene in bone remodeling and homeostasis. Mmp13b mutants exhibited impaired osteoclast activity and inability to degrade unmineralized collagens and mineralized bone matrix. The lack of mmp13b also resulted in immature and non-activated osteoclasts and delayed fin regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Abigail Y. T. Loh, Sanja Spoljar, Granville Y. W. Neo, Nathalie Escande-Beillard, Marc Leushacke, Monique N. H. Luijten, Byrappa Venkatesh, Carine Bonnard, Maurice A. M. van Steensel, Henning Hamm, Andrew Carmichael, Neil Rajan, Thomas J. Carney, Bruno Reversade
Summary: Huriez syndrome is a rare genetic skin disorder characterized by scleroatrophic hands and feet, hypoplastic nails, palmoplantar keratoderma, and a predisposition to cutaneous squamous cell carcinoma. This study reported three HRZ families from different regions and identified germline SMARCAD1 pathogenic variants. The findings suggest that Huriez syndrome may be allelic to Adermatoglyphia and Basan syndrome, sharing similar genetic and phenotypic features.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2022)
Article
Fisheries
Penghui Zhang, Tom J. Carney, Karin Schroen, Remko M. Boom, Mary B. Chan-Park
Summary: Crosslinked beads prepared by diffusion-driven layer by layer process exhibit excellent antibacterial activity and biocompatibility, with high adsorption capacity for removing fish waste.
Article
Cell Biology
Lalith Prabha Ethiraj, En Lei Samuel Fong, Ranran Liu, Madelynn Chan, Christoph Winkler, Tom James Carney
Summary: In this study, we developed simple colorimetric and fluorescent TRAP assays in zebrafish and medaka, which can be used to assess osteoclast activity. Fluorescent TRAP staining using the ELF97 substrate is a rapid, stable system that is compatible with other fluorescence stains, transgenic lines, and antibody approaches.
EUROPEAN JOURNAL OF HISTOCHEMISTRY
(2022)
Meeting Abstract
Rheumatology
E. L. S. Fong, E. L. Prabha, T. Carney
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Harsha Mahabaleshwar, P. Asharani, Tricia Yi Loo, Shze Yung Koh, Melissa R. Pitman, Samuel Kwok, Jiajia Ma, Bo Hu, Fang Lin, Xue Li Lok, Stuart M. Pitson, Timothy E. Saunders, Tom J. Carney
Summary: Mesenchymal cell immigration into growing fins and limb buds drives distal outgrowth, with tensile forces between these cells essential for fin and limb morphogenesis. Morphogens derived from the apical fin domain regulate cell polarity, migration, division, and adhesion of limb mesenchyme cells. The mutant stomp in zebrafish displays defects in fin morphogenesis, including blister formation and loss of orientation and adhesion of immigrating fin mesenchyme cells. The gene encoding Slit3, an axon guidance ligand, is found to be mutated in stomp. Slit ligands derived from immigrating mesenchyme act via Robo receptors at the apical ectodermal ridge (AER) to promote the release of sphingosine-1-phosphate (S1P), which then diffuses back to the mesenchyme to regulate their polarization, orientation, positioning, and adhesion to the interstitial matrix of the fin fold.
Article
Cell Biology
Claire A. Scott, Tom J. Carney, Enrique Amaya
Summary: The mechanisms of appendage regeneration are still largely unknown, and metabolic reprogramming plays an important role in the process. This study used a genetically encoded biosensor called Laconic to evaluate lactate levels during fin regeneration in zebrafish. The findings showed a rapid increase in lactate levels following injury, with a role of aerobic glycolysis in actomyosin contraction and wound closure. Additionally, a second wave of lactate production was observed during larval tail regeneration. Inhibiting glycolysis hindered wound contraction and tissue regrowth, indicating the necessity of aerobic glycolysis at two distinct stages of regeneration.
WOUND REPAIR AND REGENERATION
(2022)
Article
Multidisciplinary Sciences
Thang Cong Do, Jun Wei Lau, Caixia Sun, Songhan Liu, Khoa Tuan Kha, Seok Ting Lim, Yu Yang Oon, Yuet Ping Kwan, Jia Jia Ma, Yuguang Mu, Xiaogang Liu, Thomas James Carney, Xiaomeng Wang, Bengang Xing
Summary: ENCTACs, by specifically degrading BET protein BRD4 and modulating hypoxic signaling, provide a precise treatment approach for cancer.
Article
Multidisciplinary Sciences
Tatiana Subkhankulova, Karen Camargo Sosa, Leonid A. Uroshlev, Masataka Nikaido, Noah Shriever, Artem S. Kasianov, Xueyan Yang, Frederico S. L. M. Rodrigues, Thomas J. Carney, Gemma Bavister, Hartmut Schwetlick, Jonathan H. P. Dawes, Andrea Rocco, Vsevolod J. Makeev, Robert N. Kelsh
Summary: Neural crest cells are highly multipotent stem cells, and it is still unclear how their fate restriction to specific fates occurs. This study shows that in zebrafish, broad multipotency is retained even after migration, suggesting that fate restriction occurs directly but dynamically.
NATURE COMMUNICATIONS
(2023)