期刊
JOURNAL OF PROTEOME RESEARCH
卷 12, 期 4, 页码 1820-1829出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr301157j
关键词
Herpes Simplex Virus; phosphorylation; ubiquitylation; gene expression; proteome; DNA replication; late proteins; protein trafficking
资金
- Canadian Institutes of Health Research grant
- Canadian Center of Excellence in Commercialization and Research
- Canada Foundation for Innovation
- Fonds de Recherche du Quebec en Sante
- Natural Science and Engineering Research Council of Canada Vanier CGS
- German Research Foundation [RA 1608/4-1]
Herpesviruses are among the most complex and widespread human viruses and cause a number of diseases ranging from cold sores to genital infections and encephalitis. While the composition of viral particles has been studied, less is known about the expression of the whole viral proteome in infected cells. Here, we analyzed the proteome of the prototypical Herpes Simplex Virus type 1 (HSV1) in infected cells by mass spectrometry. Using a high sensitivity LTQ-Orbitrap, we achieved a very high level of protein coverage and identified a total of 67 structural and nonstructural viral proteins. We also identified 90 novel phosphorylation sites and 10 novel ubiquitylation sites on different viral proteins. Ubiquitylation was observed on nine HSV1 proteins. We identified phosphorylation sites on about half of the detected viral proteins; many of the highly phosphorylated ones are known to regulate gene expression. Treatment with inhibitors of DNA replication induced changes of both viral protein abundance and modifications, highlighting the interdependence of viral proteins during the life cycle. Given the importance of expression dynamics, ubiquitylation, and phosphorylation for protein function, these findings will serve as important tools for future studies on herpesvirus biology.
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