Article
Biotechnology & Applied Microbiology
Devender Arora, Jong-Eun Park, Dajeong Lim, Bong-Hwan Choi, In-Cheol Cho, Krishnamoorthy Srikanth, Jaebum Kim, Woncheoul Park
Summary: This study identified 16 CpG islands in liver and backfat tissues, which play important regulatory roles in differentiation processes. Differential methylation regions (DMRs) and differentially expressed genes (DEGs) on these islands indicated the differences between the two tissues. Motif analysis and gene ontology studies revealed the critical roles of these differentially methylated genes in lipid metabolism, reproductive performance, and immunity pathways.
Article
Cell Biology
Jae-Sung You, Nilmani Singh, Adriana Reyes-Ordonez, Nidhi Khanna, Zehua Bao, Huimin Zhao, Jie Chen
Summary: ARHGEF3 deficiency enhances muscle regeneration by promoting muscle quality and function, independently of the Akt signaling pathway. Furthermore, ARHGEF3 KO promotes muscle regeneration through activation of autophagy, which is critical for maintaining muscle strength and preventing muscle weakness.
Article
Multidisciplinary Sciences
Meriam Nefla, Nicola J. Darling, Manuel van Gijsel Bonnello, Philip Cohen, J. Simon C. Arthur
Summary: Salt Inducible Kinases 2 (SIK2) and 3 (SIK3) play important roles in thymic T cell development, with their loss leading to decreased numbers of peripheral T cells and defects in negative and/or positive selection in the thymus.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Suad Gazi Jaafer Husaine Al Kufi, Josiah Emmerson, Heidi Rosenqvist, Catarina Mateus Moreira Garcia, Diana Onodelia Rios-Szwed, Martin Wiese
Summary: Kinesins, including LmxKIN29, play important roles in cell division, intracellular organization, and flagellum formation and maintenance. The study suggests that LmxKIN29 is a potential drug target for leishmaniasis based on its crucial role in the disease and its divergence from mammalian kinesins.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Ramu Dandugudumula, Renana Fischer-Weinberger, Dan Zilberstein
Summary: In this study, the dynamics of microtubules during Leishmania development were investigated, revealing the importance of microtubule stabilization and rearrangement in parasite rounding and elongation processes.
Article
Materials Science, Biomaterials
Yixia Yin, Wenwu Wang, Qi Shao, Binbin Li, Dan Yu, Xin Zhou, Jayanti Parajuli, Haixing Xu, Tong Qiu, Ali Kemal Yetisen, Nan Jiang
Summary: The development of a novel neural stem cell scaffold material has shown promising results in supporting the growth and differentiation of neural stem cells, offering a new potential avenue for spinal cord injury repair.
BIOMATERIALS SCIENCE
(2021)
Review
Microbiology
Dan Zilberstein
Summary: Leishmania parasites reside in phagolysosomes of macrophages where they sense the lysosome-specific environment for differentiation initiation. Protein kinase A plays a crucial role as the gatekeeper in this process.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ali Derakhshani, Iraj Sharifi, Ehsan Salarkia, Alireza Keyhani, Setareh Agha Kuchak Afshari, Behzad Iranmanesh, Mahdieh Lashkarizadeh, Hamid Nejad Biglari, Moslem Lari Najafi, Mehdi Bamorovat
Summary: This study investigated the antileishmanial activity of azacitidine (AZA) on Leishmania major in vitro and in silico models. The results showed that AZA could interact with iNOS and the combination of AZA and MA was more effective in inhibiting the growth rate of promastigotes and amastigotes. Furthermore, the combination of AZA and MA promoted immune response, particularly the Th1 pathway.
Article
Physics, Multidisciplinary
Yunfang Ma, Jose M. Sallan, Oriol Lordan
Summary: This study constructs complex network models based on the Beijing rail transit real network using space P and space L methods, and analyzes the topological structure and global characteristics of the line and station networks. It also obtains all motifs from 3 to 8 nodes and identifies different typical subgraphs for the two networks of Beijing rail transit. The research proposes a method to assess the connectivity of the rail transit system and has significant reference value for optimizing network functions in the design and planning of rail transit networks.
PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIONS
(2023)
Article
Multidisciplinary Sciences
Zhengyu Qian, Jingwei Ye, Jinteng Li, Yunshu Che, Wenhui Yu, Peitao Xu, Jiajie Lin, Feng Ye, Xiaojun Xu, Zepeng Su, Dateng Li, Zhongyu Xie, Yanfeng Wu, Huiyong Shen
Summary: This study aimed to explore the role of crotonylation in myogenic differentiation and its potential as a target in myogenic dysfunction diseases. Through in vitro and in vivo experiments, it was confirmed that crotonylation affects muscle differentiation and the crotonylation eraser HDAC3 regulates myogenic differentiation. The findings contribute to a better understanding of the pathophysiological processes of muscle injury repair and sarcopenia.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Cell Biology
Helene Hoenigsperger, Lennart Koepke, Dhiraj Acharya, Victoria Hunszinger, Dennis Freisem, Alexander Grenzner, Sebastian Wiese, Frank Kirchhoff, Michaela U. Gack, Konstantin M. J. Sparrer
Summary: This study identifies CSNK2/CK2 as a negative regulator of autophagy, which can limit autophagy by interacting with TRIM family members. CSNK2 inhibits autophagy by phosphorylating TRIM3. This finding is significant for the treatment of diseases and viral infections associated with dysregulated autophagy.
Article
Multidisciplinary Sciences
Asako Goto, Shota Sakai, Aya Mizuike, Toshiyuki Yamaji, Kentaro Hanada
Summary: Casein kinase 1 gamma (CK1G) plays a role in regulating cellular functions and has been found to inactivate the ceramide transport protein (CERT) through phosphorylation. In this study, it was discovered that loss of the C-terminal region of CK1G3 led to hyperactivation of the kinase and redistribution within the cell. These findings suggest that CK1Gs are regulated through compartmentalization to access specific intracellular organelles.
Article
Biochemistry & Molecular Biology
Mutsuki Amano, Yoko Kanazawa, Kei Kozawa, Kozo Kaibuchi
Summary: This study analyzed the interaction between Rho-kinase and MYPT1 and found that three short regions of MYPT1 close to the phosphorylation sites are crucial for the interaction with Rho-kinase. The phosphorylation levels of MYPT1 without these regions were reduced and only affected neighboring phosphorylation sites. Furthermore, the combination of pseudosubstrate (PS) and docking motifs (DMs) of MYPT1 was found to be a potent inhibitor of Rho-kinase.
Article
Immunology
Tanvir Bamra, Taj Shafi, Sushmita Das, Manjay Kumar, Manas Ranjan Dikhit, Ajay Kumar, Ashish Kumar, Kumar Abhishek, Krishna Pandey, Abhik Sen, Pradeep Das
Summary: Leishmania parasite secretes numerous proteins during in vitro cultivation, with Mevalonate kinase playing a significant role in infection. Visceral Leishmaniasis is a persistent infection caused by Leishmania donovani, partly due to immune evasion. The novel secretory protein MVK aids in parasite internalization and modulation of the host immune response through signal transduction pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martina Wirth, Stephane Mouilleron, Wenxin Zhang, Eva Sjottem, Yakubu Princely Abudu, Ashish Jain, Hallvard Lauritz Olsvik, Jack-Ansgar Bruun, Minoo Razi, Harold B. J. Jefferies, Rebecca Lee, Dhira Joshi, Nicola O'Reilly, Terje Johansen, Sharon A. Tooze
Summary: Autophagy is a crucial degradative pathway for maintaining cellular homeostasis, with ATG8 proteins playing a central role in autophagosome formation and cytoplasmic cargo delivery. The binding specificity of ATG8 proteins with autophagy adaptors and receptors involves LIR motifs and phosphorylation. This study reveals the binding of a LIR motif in the Golgi protein SCOC to GABARAP, GABARAPL1, LC3A and LC3C, with phosphorylation regulating the affinity of LC3 family binding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Medicinal
Lyamin Z. Bendjeddou, Nadege Loaec, Benoit Villiers, Eric Prina, Gerald F. Spath, Herve Galons, Laurent Meijer, Nassima Oumata
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Editorial Material
Immunology
Fariborz Bahrami, Gerald F. Spath, Sima Rafati
EXPERT REVIEW OF VACCINES
(2017)
Article
Ecology
Pablo Prieto Barja, Pascale Pescher, Giovanni Bussotti, Franck Dumetz, Hideo Imamura, Darek Kedra, Malgorzata Domagalska, Victor Chaumeau, Heinz Himmelbauer, Michel Pages, Yvon Sterkers, Jean-Claude Dujardin, Cedric Notredame, Gerald Frank Spath
NATURE ECOLOGY & EVOLUTION
(2017)
Article
Biochemical Research Methods
Luca Parca, Bruno Ariano, Andrea Cabibbo, Marco Paoletti, Annalaura Tamburrini, Antonio Palmeri, Gabriele Ausiello, Manuela Helmer-Citterich
Article
Biochemistry & Molecular Biology
T. Liburkin-Dan, D. Schlisselberg, R. Fischer-Weinberger, P. Pescher, E. Inbar, M. Ephros, D. Rentsch, G. F. Spath, D. Zilberstein
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2018)
Review
Immunology
Laura Piel, Pascale Pescher, Gerald F. Spath
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Microbiology
Giovanni Bussotti, Evi Gouzelou, Mariana Cortes Boite, Ihcen Kherachi, Zoubir Harrat, Naouel Eddaikra, Jeremy C. Mottram, Maria Antoniou, Vasiliki Christodoulou, Aymen Bali, Fatma Z. Guerfali, Dhafer Laouini, Maowia Mukhtar, Franck Dumetz, Jean-Claude Dujardin, Despina Smirlis, Pierre Lechat, Pascale Pescher, Adil El Hamouchi, Meryem Lemrani, Carmen Chicharro, Ivonne Pamela Llanes-Acevedo, Laura Botana, Israel Cruz, Javier Moreno, Fakhri Jeddi, Karim Aoun, Aida Bouratbine, Elisa Cupolillo, Gerald F. Spath
Article
Multidisciplinary Sciences
S. Lamotte, N. Aulner, G. F. Spath, E. Prina
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
S. Lamotte, N. Aulner, G. F. Spath, E. Prina
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Thibault Rosazza, Herve Lecoeur, Thierry Blisnick, Maryse Moya-Nilges, Pascale Pescher, Phillipe Bastin, Eric Prina, Gerald F. Spath
Summary: This study analyzed the response of Leishmania parasites to pyroptosis in host cells using high-content real-time imaging. It was found that during pyroptosis, the parasite's fate changed within the host cell, potentially aiding in parasite dissemination.
JOURNAL OF CELL SCIENCE
(2021)
Article
Genetics & Heredity
Giovanni Bussotti, Alia Benkahla, Fakhri Jeddi, Oussama Souiai, Karim Aoun, Gerald F. Spath, Aida Bouratbine
MICROBIAL GENOMICS
(2020)
Review
Parasitology
Herve Lecoeur, Eric Prina, Maria Gutierrez-Sanchez, Gerald F. Spaeth
Summary: Intracellular parasites have evolved complex strategies to disrupt host cell functions and ensure their own survival. Leishmania parasites, in particular, cause severe immunopathologies by thriving inside immune cells. While the impact of Leishmania infection on host cell phenotype and functions has been studied, the regulatory mechanisms underlying host cell subversion were only recently investigated.
TRENDS IN PARASITOLOGY
(2022)
Article
Biology
Marie Villares, Nelly Lourenco, Jeremy Berthelet, Suzanne Lamotte, Leslie Regad, Souhila Medjkane, Eric Prina, Fernando Rodrigues-Lima, Gerald F. Spath, Jonathan B. Weitzman
Summary: A study finds that Trifloxystrobin is an effective alternative to Buparvaquone for treating Theileria parasites infection. Trifloxystrobin inhibits parasite survival and differentiation, and has similar effects on host gene expression, cell proliferation, and cell cycle as Buparvaquone.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Giovanni Bussotti, Blaise Li, Pascale Pescher, Barbora Vojtkova, Isabelle Louradour, Katerina Pruzinova, Jovana Sadlova, Petr Volf, Gerald F. Spath
Summary: This study investigates the genomic adaptation of the parasitic pathogen Leishmania donovani during sand fly infection. The findings suggest that oxidative DNA damage and DNA repair processes contribute to haplotype and allelic selection in the parasite.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Marco Pietrosanto, Marta Adinolfi, Riccardo Casula, Gabriele Ausiello, Fabrizio Ferre, Manuela Helmer-Citterich