期刊
JOURNAL OF PROTEOME RESEARCH
卷 11, 期 10, 页码 4834-4846出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr300274k
关键词
ativiral phagocytosis; actin; Rab6; invertebrate
资金
- National Natural Science Foundation of China [30830084]
- Hi-Tech Research and Development Program of China (863 program of China) [2010AA09Z403]
- Ministry of Agriculture, China [201103034]
Phagocytosis, which is of fundamental importance for innate and adaptive immunity in animals, is driven by organization of the actin cytoskeleton. To date, however, the molecular events involved in the regulation of phagocytosis through reorganization of actin by small G proteins remains to be elucidated. To address this issue, the molecular mechanism of Rab6 in phagocytosis against virus infection in invertebrates was characterized in this study. The results showed that the Rab6 obtained from shrimp could interact with actin to regulate shrimp hemocyte phagocytosis through induction of the rearrangement of actin to protect against white spot syndrome virus (WSSV) infection. The Rab6 protein in Drosophila melanogaster shared the same mechanism of action as that of Rab6 in shrimp, indicating that the function of Rab6 in phagocytosis was conserved in invertebrates. By comparison with the early marker (Rab5) and late marker (LAMP I) of phagosomes, Rab6 was critically involved in the regulation of actin organization throughout the entire phagocytosis process. The presence of the evolutionarily conserved amino acid sequences of Rab6 in invertebrates and vertebrates indicated a conserved mechanism of Rab6 function in phagocytosis of animals. Therefore, our findings presented novel molecular events in the regulation of phagocytosis by small G proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据