Article
Environmental Sciences
Gefei Huang, Yiming Ma, Dongying Xie, Cunmin Zhao, Lin Zhu, Guangshan Xie, Pengfei Wu, Wei Wang, Zhongying Zhao, Zongwei Cai
Summary: Plastic pollution poses a significant threat to ecosystems. While the adverse effects of microplastics have been extensively studied, the toxicity of nanoplastics remains unclear, particularly at the protein level. This study used quantitative proteomics to investigate the proteome dynamics in a soil nematode exposed to 100 nm polystyrene nanoplastics (PS-NPs). The results showed that exposure to PS-NPs led to differential expression of proteins related to various cellular processes, and these changes were consistent with the observed phenotypic defects. Overall, this study provides insights into the molecular mechanisms underlying the toxicity of nanoplastics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Cell Biology
Khairun Nisaa, Anat Ben-Zvi
Summary: Muscle proteostasis is regulated by the myogenic transcription factor MyoD. This study demonstrates that MyoD can modulate chaperone expression in terminally differentiated muscle cells, indicating its role in muscle maintenance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rocco J. Rotello, Timothy D. Veenstra
Summary: In the era of genomics research, mass spectrometry has become a vital tool for quantitatively comparing proteins within cells and organisms, despite the complexity of identifying quantitative changes in proteomes due to their dynamic nature and limitations in technology coverage. The combined development of sample preparation and MS methods has enabled the quantitation of thousands of proteins obtained from cells and organisms.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Review
Chemistry, Analytical
Negin Fasih Ramandi, Mohammad Faranoush, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The recent success in studying the proteome as a source of biomarkers has revolutionized our understanding of leukemia. The use of mass spectrometry techniques to identify differentially expressed proteins has led to improved diagnosis, prognosis, and treatment strategies for leukemia. This review focuses on the use of various ionization sources and mass analyzers in monitoring different types of leukemia, with MALDI-TOF MS identified as a quick and reliable method for protein analysis. Additionally, the review classifies over 400 proteins and identifies specific biomarkers for acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and other stages of leukemia for future studies.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Jing Pu, Bingye Zhao, Xin Liu, Shugang Li, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: This study comprehensively compared the protein profiles and properties of chicken egg white and its three components. The proteomes of thick and thin egg white are similar, but thick egg white has higher abundance of ovomucin subunits and lysozymes. The properties of thick and thin egg white are significantly different. Additionally, the chalaza component has higher abundance of insoluble proteins and lower abundance of soluble proteins, which explains its insolubility. These findings are important for further research and development of egg white, such as thinning of egg white, understanding changes in egg white properties, and differential application of thick and thin egg white.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Omer Yuval, Yael Iosilevskii, Anna Meledin, Benjamin Podbilewicz, Tom Shemesh
Summary: Complex dendritic trees are a distinctive feature of neurons, and alterations in dendritic morphology are associated with developmental, behavioral, and neurodegenerative changes. Using deep-learning and fitting algorithms, a method for neuronal feature extraction was presented, revealing uneven distribution of dendritic junctions and three-way-symmetrical junctions on average in the PVD neuron of C. elegans. Further analysis showed localized reduction in junctions due to mutations, shedding new light on PVD architecture and suggesting molecular control mechanisms.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Dianqin Xu, Xiaoyu Zhu, Ji Ren, Shan Huang, Ziwen Xiao, Hongmei Jiang, Yujie Tan
Summary: In this study, tandem mass tags-labeled quantitative proteomic technology and bioinformatics tools were used to analyze exfoliated cervical cells from normal and cervical cancer groups. A cancer-specific protein profile was established, leading to the identification of key proteins related to cervical cancer development. These differentially expressed proteins were found to be involved in various cellular processes and signaling pathways, laying the foundation for understanding the molecular mechanisms of cervical cancer.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Max K. Steinbach, Jan Leipert, Christine Blurton, Matthias Leippe, Andreas Tholey
Summary: This study demonstrates a highly sensitive sample preparation workflow using digital microfluidics and high-resolution mass spectrometry for nanoproteomics research.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Tao Ding, Ye-Hong Yang, Qiao-Chu Wang, Yue Wu, Rong Han, Xu-Tong Zhang, Jie Kong, Jun-Tao Yang, Jiang-Feng Liu
Summary: Lactylation, a novel posttranslational modification, plays a crucial role in studying protein functions, disease mechanisms, and conservation across different organisms. This study investigates the lactylation of global proteins in Caenorhabditis elegans and establishes the first lactylome database for this organism.
Article
Agricultural Engineering
Shan Huang, Qingxu Ma, Qiong Hou, Ting Zuo, Zhijian Zhang, Wuzhong Ni
Summary: The bioconversion of organic waste can increase betaine content in compost, while vermicomposting tends to reduce betaine levels. Larvae bioconversion shows a more significant increase in betaines in compost.
BIORESOURCE TECHNOLOGY
(2021)
Review
Genetics & Heredity
Xiulan Chen, Yaping Sun, Tingting Zhang, Lian Shu, Peter Roepstorff, Fuquan Yang
Summary: Isobaric labeling technology has become a key tool for comparing protein expression in biological samples, although challenges remain in terms of quantitative accuracy and precision. The different types of isobaric mass tags and advantages and disadvantages of the labeling method are detailed, along with precautions needed at each step of the workflow for reliable quantification results. The broad applications of isobaric labeling technology in biological and clinical studies, with a focus on thermal proteome profiling and proteogenomics, are also discussed.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2021)
Article
Microbiology
Ganeshwari Dhurve, Ashok Kumar Madikonda, Medicharla Venkata Jagannadham, Dayananda Siddavattam
Summary: This study reveals the presence of proteins in the outer membrane vesicles (OMVs) of Acinetobacter baumannii DS002 that are involved in selective biological functions. The study identifies TonB-dependent transporters (TonRs) as predominant proteins involved in inorganic ion transport and metabolism in OMVs. The OMVs also carry proteins known to promote pathogenesis, immune evasion, and biofilm formation. These findings provide conclusive evidence for the important role of OMVs in nutrient transport, particularly iron, and highlight the presence of proteins involved in pathogenicity and immune response.
MICROBIOLOGY SPECTRUM
(2022)
Article
Geriatrics & Gerontology
Hongru Lin, Yehui Gao, Chen Zhang, Botian Ma, Mengchen Wu, Xianghuan Cui, Hongbing Wang
Summary: Alzheimer's disease (AD) is a neurodegenerative disease characterized by the accumulation of A β proteins, leading to proteostasis imbalance. Autophagy deficits have been found in AD. Using transgenic C. elegans, it was discovered that reducing autophagosome accumulation can delay paralysis and alleviate toxicity induced by A β. RNA-sequencing and proteomics identified autophagy-related signal pathways affected by A β aggregation in C. elegans.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Food Science & Technology
Ana G. Abril, Monica Carrera, Karola Boehme, Jorge Barros-Velazquez, Benito Canas, Jose-Luis R. Rama, Tomas G. Villa, Pilar Calo-Mata
Summary: This study utilized LC-ESI-MS/MS MS to analyze peptides from bacteriophages infecting mastitis-causing Staphylococcus aureus, identifying a large number of specific peptides that could be useful for identifying and characterizing the bacteria causing mastitis. Additionally, a study of bacteriophage phylogeny and its relationship with the respective Staphylococcus spp. infected was conducted, revealing specific peptides present in closely related phages and highlighting the connection between bacteriophage phylogeny and the infected Staphylococcus spp.
Article
Chemistry, Analytical
Xiaoling Su, Deying Chen, Liang Li, Lanjuan Li
Summary: The study demonstrates that perfusion can significantly alter the liver metabolome, recommending the use of non-perfused liver for comprehensive liver metabolomics analysis for high coverage.
ANALYTICA CHIMICA ACTA
(2021)
Article
Oncology
Marina Maslova, Heinz Herden, Karin Schork, Michael Turewicz, Martin Eisenacher, Roland Schroers, Alexander Baraniskin, Thomas Mika
Summary: This study retrospectively analyzed the ceCT-based density of LARC in 30 patients after neoCRT, finding a decrease in density overall post-neoCRT, with the most significant decrease in patients achieving pCR. Densitometry showed a high specificity and sensitivity for predicting pCR, suggesting its usefulness in selecting patients for a watch-and-wait strategy and warranting further prospective studies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yasset Perez-Riverol, Jingwen Bai, Chakradhar Bandla, David Garcia-Seisdedos, Suresh Hewapathirana, Selvakumar Kamatchinathan, Deepti J. Kundu, Ananth Prakash, Anika Frericks-Zipper, Martin Eisenacher, Mathias Walzer, Shengbo Wang, Alvis Brazma, Juan Antonio Vizcaino
Summary: PRIDE is the largest data repository of mass spectrometry-based proteomics data in the world, with around 500 datasets submitted per month. In addition to continuous improvements in data pipelines and infrastructure, PRIDE has developed the Spectra Archive and MAGE-TAB file format to enhance sample metadata annotation and access to mass spectra.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jonas Schuhenn, Toni Luise Meister, Daniel Todt, Thilo Bracht, Karin Schork, Jean-Noel Billaud, Carina Elsner, Natalie Heinen, Zehra Karakoese, Sibylle Haid, Sriram Kumar, Linda Brunotte, Martin Eisenacher, Yunyun Di, Jocelyne Lew, Darryl Falzarano, Jieliang Chen, Zhenghong Yuan, Thomas Pietschmann, Bettina Wiegmann, Hendrik Uebner, Christian Taube, Vu Thuy Khanh Le-Trilling, Mirko Trilling, Adalbert Krawczyk, Stephan Ludwig, Barbara Sitek, Eike Steinmann, Ulf Dittmer, Kerry J. Lavender, Kathrin Sutter, Stephanie Pfaender
Summary: This study found that different subtypes of IFN-alpha have varying antiviral activity against SARS-CoV-2, with IFN-alpha 5 showing the highest efficacy. Combination treatment with the broad antiviral drug remdesivir enhanced the antiviral effect. These findings are important for understanding the mechanism of IFN-I-mediated antiviral response and developing treatments for COVID-19.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Anesthesiology
Matthias Unterberg, Tim Rahmel, Katharina Rump, Alexander Wolf, Helge Haberl, Alexander von Busch, Lars Bergmann, Thilo Bracht, Alexander Zarbock, Stefan Felix Ehrentraut, Christian Putensen, Frank Wappler, Thomas Koehler, Bjorn Ellger, Nina Babel, Ulrich Frey, Martin Eisenacher, Daniel Kleefisch, Katrin Marcus, Barbara Sitek, Michael Adamzik, Bjorn Koos, Hartmuth Nowak, Michael Adamzik, Moritz Anft, Thorsten Annecke, Maha Bazzi, Lars Bergmann, Christian Bode, Jerome M. Defosse, Stefan F. Ehrentraut, Christian Ertmer, Ulrich H. Frey, Katrin Fuchs, Helge Haberl, Dietrich Henzler, Ulrich Limper, Katrin Marcus, Hartmuth Nowak, Daniel Oswald, Christian Putensen, Tim Rahmel, Katharina Rump, Jens-Christian Schewe, Elke Schwier, Barbara Sitek, Matthias Unterberg, Frank Wappler, Katrin Willemsen, Alexander Wolf, Alexander Zarbock, Birgit Zuelch
Summary: The COVID-19 pandemic has led to increased mortality of non-COVID-19 sepsis patients, potentially due to a higher disease severity at the time of recruitment. However, no evidence was found for a diminished quality of care for critically ill sepsis patients in German intensive care units during the pandemic.
BMC ANESTHESIOLOGY
(2022)
Article
Clinical Neurology
Matthias Hoellerhage, Markus Stepath, Michael Kohl, Kathy Pfeiffer, Oscar Wing ho Chua, Linghan Duan, Franziska Hopfner, Martin Eisenacher, Katrin Marcus, Guenter U. Hoeglinger
Summary: LUHMES cells can serve as a model to investigate the pathophysiological processes involved in Parkinson's disease, with research focusing on identifying genes and proteins related to early cellular signaling pathways associated with cell death. The study found differential regulation of genes and proteins associated with Parkinson's disease in the cell model.
FRONTIERS IN NEUROLOGY
(2022)
Article
Clinical Neurology
Maximilian Wulf, Katalin Barkovits, Karin Schork, Martin Eisenacher, Peter Riederer, Manfred Gerlach, Britta Eggers, Katrin Marcus
Summary: Neuromelanin is a pigment present in neuromelanin granules (NMGs) in dopaminergic neurons. The function, origin, and synthesis of NMGs are still not fully understood. The study found a link between NMGs and lysosomes, as well as a high abundance of tyrosine hydroxylase in NMGs, which may be involved in neuromelanin synthesis. Additionally, a previously unknown connection between stress granules (SGs) and NMGs was discovered, potentially caused by oxidative stress from neuromelanin-bound metals.
JOURNAL OF NEURAL TRANSMISSION
(2022)
Review
Biochemistry & Molecular Biology
Nils Hoffmann, Gerhard Mayer, Canan Has, Dominik Kopczynski, Fadi Al Machot, Dominik Schwudke, Robert Ahrends, Katrin Marcus, Martin Eisenacher, Michael Turewicz
Summary: Mass spectrometry is an important technology for biomedical research. This study focuses on the analysis of lipidomics data using mass spectrometry, and provides a catalog of software tools and resources available for this purpose. The study also assesses the support of standardized data formats in lipidomics workflows, and discusses the challenges and potential areas for improvement in the current tools and standards.
Article
Multidisciplinary Sciences
Julian Uszkoreit, Katalin Barkovits, Sandra Pacharra, Kathy Pfeiffer, Simone Steinbach, Katrin Marcus, Martin Eisenacher
Summary: In this article, a data dependent acquisition (DDA) dataset is presented, which serves as a reference and ground truth quantitative dataset. The dataset can be used as a benchmark reference for any workflows working on DDA data and has the potential to be valuable in comparing samples measured with DDA and data independent acquisition (DIA). The dataset consists of 15 LC-MS/MS measurements with five distinct spike-in states, each with three replicates.
Article
Biochemistry & Molecular Biology
Thilo Bracht, Daniel Kleefisch, Karin Schork, Kathrin E. Witzke, Weiqiang Chen, Malte Bayer, Jan Hovanec, Georg Johnen, Swetlana Meier, Yon-Dschun Ko, Thomas Behrens, Thomas Bruning, Jana Fassunke, Reinhard Buettner, Julian Uszkoreit, Michael Adamzik, Martin Eisenacher, Barbara Sitek
Summary: Chronic obstructive pulmonary disease (COPD) is a major risk factor for the development of lung adenocarcinoma (AC). In this study, mass-spectrometry-based proteomics analysis and machine learning algorithms were used to identify potential biomarkers for differentiation between AC and COPD. The results showed promising performance for distinguishing AC from COPD and AC with COPD from COPD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Karin Schork, Michael Turewicz, Julian Uszkoreit, Jorg Rahnenfuhrer, Martin Eisenacher
Summary: In this study, we conducted a comprehensive analysis of bipartite graphs representing the relationship between proteins and peptides in bottom-up proteomics. We compared graphs using quantified peptides from measured data sets and theoretical peptides from in-silico digestion. The results showed that the accepted minimum peptide length during in-silico digestion had a significant influence on the graph structures. When using measured peptides, the graphs were smaller and less complex compared to theoretical peptides, but there was a higher proportion of protein nodes without unique peptides. The structure of bipartite peptide-protein graphs varied between database and quantitative level as well as between different species, highlighting the need for novel methods to quantify proteins without unique peptides.
Article
Multidisciplinary Sciences
Jasjot Singh, Hadeer Elhabashy, Pathma Muthukottiappan, Markus Stepath, Martin Eisenacher, Oliver Kohlbacher, Volkmar Gieselmann, Dominic Winter
Summary: Lysosomes are crucial organelles for degradation and metabolism regulation. Protein complexes play a decisive role in lysosomal function. This study investigates lysosomes and early endosomes using cross-linking mass spectrometry, revealing protein interactions, structures, and cargo of flotillin-mediated endocytosis.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Maximilian Wulf, Katalin Barkovits, Karin Schork, Martin Eisenacher, Peter Riederer, Manfred Gerlach, Britta Eggers, Katrin Marcus
Summary: Neuromelanin granules (NMGs) may play an important role in neurodegenerative processes, but their exact mechanism is not fully understood. DLB patients show evidence of ongoing oxidative damage and impaired protein degradation in NMG-surrounding tissue. Enrichment of specific proteins in NMGs suggests their involvement in pathogenesis, but further research is needed to determine the exact role of NMGs.
Article
Biochemistry & Molecular Biology
Svitlana Rozanova, Julian Uszkoreit, Karin Schork, Bettina Serschnitzki, Martin Eisenacher, Lars Toenges, Katalin Barkovits-Boeddinghaus, Katrin Marcus
Summary: Proteomic studies using mass spectrometry (MS)-based quantification are important for discovering new biomarkers, but the accuracy of the outcomes can be affected by various analytical conditions. Therefore, it is crucial to assess the quality of the acquired data, including raw data, identification, and quantification levels. This study demonstrates the assessment of four independent datasets from cerebrospinal fluid, highlighting the impact of sample processing-based differences on the quality of quantitative data. Additionally, a free and open source quality control tool called MaCProQC is provided for systematic and rapid data comparison.
Article
Biochemistry & Molecular Biology
Robert Hardt, Alireza Dehghani, Carmen Schoor, Markus Goedderz, Nur Cengiz Winter, Shiva Ahmadi, Ramesh Sharma, Karin Schork, Martin Eisenacher, Volkmar Gieselmann, Dominic Winter
Summary: In this study, we used mass spectrometry to investigate the differentiation of pluripotent neural stem cells (NSCs) into oligodendrocytes. We identified Dclk1 as one of the key proteins involved in this process and found that it undergoes a gradual transition between its short and long isoforms during NSC to OPC differentiation. Through interactome analyses, we characterized the interaction partners and substrates of different Dclk1 isoforms. The data from this study are available on ProteomeXchange with identifier PXD040652.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Britta Eggers, Karin Schork, Michael Turewicz, Katalin Barkovits, Martin Eisenacher, Rolf Schroder, Christoph S. Clemen, Katrin Marcus
Summary: Research on skeletal muscle fiber types has revealed unique protein profiles with distinct metabolic properties and multifunctionality. Additionally, proteins associated with myopathy were found to be enriched in specific fiber types, providing valuable insights for disease studies.