Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Editorial Material
Biochemical Research Methods
Xiaofeng Wu
Summary: In this study, the researchers comprehensively analyzed the lactylproteome in hepatocellular carcinoma (HCC) tissues for the first time. They found that the lactylation modification plays an important role in the formation and metastasis of HCC, and identified two lactylation sites as diagnostic indicators for HCC. This study is of great significance for the understanding, diagnosis, and targeted therapies of HCC.
Article
Biochemical Research Methods
Bas Sleumer, Martijn van Faassen, Michel J. Vos, Rainer Bischoff, Ido P. Kema, Nico C. van de Merbel
Summary: The human growth hormone GH1 (22 kDa) is an important biomarker for growth disorders. A LC-MS/MS method was developed to accurately quantify GH1 and distinguish it from other isoforms. The method showed good performance in different sample types and storage conditions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Ondrej Kuda, Oliver Fiehn, Tomas Cajka
Summary: Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts was investigated over a three-day period under various temperature conditions. Results showed that storing dry extracts at -24 degrees C provided comparable results to -80 degrees C, but increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 degrees C and +30 degrees C.
Article
Medicine, General & Internal
Aleksandra Turek-Jakubowska, Janusz Debski, Maciej Jakubowski, Ewa Szahidewicz-Krupska, Jakub Gawrys, Karolina Gawrys, Agnieszka Janus, Malgorzata Trocha, Adrian Doroszko
Summary: This study used dynamic LC/MS technology to analyze the changes in serum proteome of patients with acute ischemic stroke, and compared them with a control group. The results identified 10 proteins with significantly different serum concentrations between the two groups of volunteers.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemical Research Methods
Fatemeh Aghai, Sebastian Zimmermann, Max Kurlbaum, Pius Jung, Theo Pelzer, Hartwig Klinker, Nora Isberner, Oliver Scherf-Clavel
Summary: A liquid chromatography tandem mass spectrometry method has been developed and validated for the analysis of ten kinase inhibitors in human serum and plasma, meeting the validation guidelines of regulatory agencies. The method allows for rapid and sensitive monitoring of kinase inhibitor concentrations in patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tobias Schlappack, Matthias Rainer, Nikolaus Weinberger, Gunther K. Bonn
Summary: This study describes the use of modified halloysite nanotubes as a novel solid material for the efficient solid phase extraction of toxic pyrrolizidine alkaloids. The optimized method showed high extraction efficiencies and overall recoveries for a mixture of pyrrolizidine alkaloids using UHPLC-MS/MS analysis. The novel solid phase material exhibited high selectivity and competitiveness compared to a commercial procedure.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Hong Y. Pan, Qing Q. Wu, Qiao Q. Yin, Yi N. Dai, Yi C. Huang, Wei Zheng, Tian C. Hui, Mei J. Chen, Ming S. Wang, Jia J. Zhang, Hai J. Huang, Yong X. Tong
Summary: Chronic hepatitis B virus infection is a primary risk factor for hepatocellular carcinoma development. This study used LC-MS to analyze serum metabolic profiles of CHB, LC, and HCC patients, identifying metabolites that change with liver disease progression. Certain metabolites, such as TCA and eicosapentaenoic acid, were found to have diagnostic performance with liver disease progression and are correlated with AFP levels and age.
Article
Environmental Sciences
Garth Campbell, Andrea Mannetje, Samuel Keer, Geoff Eagleshama, Xianyu Wang, Chun-Yin Lin, Peter Hobson, Leisa-Maree Toms, Jeroen Douwes, Kevin V. Thomas, Jochen F. Mueller, Sarit Kaserzon
Summary: This study analyzed urine samples from the Australia and New Zealand region and obtained data on the concentrations of glyphosate and its degradation product aminomethylphosphonic acid (AMPA). The results showed that glyphosate was detected in 8% of the urine samples from the Australian general population and in 96% of urine samples from New Zealand farmers. This indicates a widespread presence of glyphosate in the population, with higher detections among farmers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Margaret L. Eng, Chunyan Hao, Christena Watts, Fengrong Sun, Christy A. Morrissey
Summary: The study utilized sensitive analytical methods to measure the effects of two field-realistic concentrations of neonicotinoid insecticides on red-winged blackbirds. Imidacloprid was rapidly absorbed and metabolized within 48 hours, with the most significant decrease in parent IMI observed at 6 hours post-exposure. 5-OH-IMI was identified as a persistent marker of exposure at 48 hours.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Yumi Komori, Tomoya Niinae, Koshi Imami, Jun Yanagibayashi, Kenichi Yasunaga, Shinya Imamura, Masami Tomita, Yasushi Ishihama
Summary: We developed a bioinertized nanoflow LC/MS/MS system for sensitive analysis of phosphopeptides. Metal ion depletion from the mobile phase and prevention of indirect contact with metal components significantly improved the recovery of phosphopeptides. By inserting an online metal ion removal device, the identified phosphopeptides showed an average increase in peak areas of 9.9-fold overall and 77-fold for multiply phosphorylated peptides.
MOLECULAR & CELLULAR PROTEOMICS
(2023)