期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 8, 页码 3399-3417出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr101299j
关键词
proteomics; Acinetobacter baumannii; biofilms; histidine; salicylate; 2DE; iTRAQ; MALDI-TOF/TOF
资金
- FCT-MCTES [SFRH/BD/6474/2009]
- Instituto de Salud Carlos III
- Xunta de Galicia [FIS PI081613, PS09/00687, PS07/90, PS07/51, 08CSA064916PR]
- Spanish Network for the Research in Infectious Diseases [REIPI RD06/0008]
Biofilm formation is one of the main causes for the persistence of Acinetobacter baumannii, a pathogen associated with severe infections and outbreaks in hospitals. Here, we performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compound (salicylate). Using this multiple-approach strategy, proteomic patterns showed a unique lifestyle for A. baumannii biofilms and novel associated proteins. Several cell surface proteins (such as CarO, OmpA, OprD-like, DcaP-like, PstS, LysM, and Omp33), as well as those involved in histidine metabolism (like Urocanase), were found to be implicated in biofilm formation, this being confirmed by gene disruption. Although L-His uptake triggered biofilms efficiently in wild-type A. baumannii, no effect was observed in Urocanase and OmpA mutants, while a slight increase was observed in a Car deficient strain. We conclude that Urocanase plays a crucial role in histidine metabolism leading to biofilm formation and that OmpA and Car can act as channels for L-His uptake. Finally, we propose a model in which novel proteins are suggested for the first time as targets for preventing the formation of A. baumannii biofilms.
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