期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 3, 页码 1374-1382出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr101138m
关键词
metabolomic fingerprinting; LC-QTOF-MS; abdominal aortic aneurysm; secretome
资金
- CAM [S2006/GEN-0247]
- European Community [HEALTH F2-2008-200647]
- Spanish Ministerio de Ciencia y Tecnologia [SAF2007/63648, CTQ2008-03779]
- Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RECAVA [RD06/0014/0035, EADSCASA, EUS2008-03565]
Abdominal aortic aneurysm (AAA) is permanent and localized dilation of the abdominal aorta. Intraluminal thrombus (ILT) is involved in evolution and rupture of AAA. Complex biological processes associated with AAA include oxidative stress, proteolysis, neovascularization, aortic inflammation, cell death, and extracellular matrix breakdown. Biomarkers of growth and AAA rupture could give a more nuanced indication for surgery, unveil novel pathogenic pathways, and open possibilities for pharmacological inhibition of growth. Differential analysis of metabolites released by normal and pathological arteries in culture may help to find molecules that have a high probability of later being found in plasma and start signaling processes or be useful diagnostic/prognostic markers. We used a LC-QTOF-MS metabolomic approach to analyze metabolites released by human ILT (divided into luminal and abluminal layers), aneurysm wall (AW), and healthy wall (I-LW). Statistical analysis was used to compare luminal with abluminal ILT layer, ILT with AW, and AW with MW to select the metabolites exchanged between tissue and external medium. Identified compounds are related to inflammation and oxidative stress and indicate the possible role of fatty acid amides in AAA. Some metabolites (e.g., hippuric acid) had not been previously associated to aneurysm, others (fatty acid amides) have arisen, indicating a very promising line of research.
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