Article
Agriculture, Multidisciplinary
Wenyuan Zhang, Jing Lu, Baorong Chen, Peng Gao, Bo Song, Shuwen Zhang, Xiaoyang Pang, Kasper Hettinga, Jiaping Lyu
Summary: In this study, milk samples from 10 cows in different lactation stages were collected to investigate the differential expression of whey proteins and N-glycoproteins. A total of 240 whey proteins and 315 N-glycosylation sites on 214 glycoproteins were identified. GO annotations, KEGG pathway analysis, and protein classification were conducted to understand the similarities and differences in biological functions between whey proteins and N-glycoproteins across different lactation stages. The study also found differential expression of whey proteins and whey N-glycosylated proteins between lactation stages, and discussed the related changes in biological functions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Zhen Feng, Yan Shen, Gongjian Fan, Tingting Li, Caie Wu, Yuhui Ye
Summary: This study reveals the subtle differences in protein compositions of bovine colostrum, first lactation milk, and second lactation milk, contributing to the development of protein ingredients for infant formula.
Article
Agriculture, Dairy & Animal Science
Mohan Li, Kexin Zheng, Wanying Song, Haikun Yu, Xiumin Zhang, Xiqing Yue, Qilong Li
Summary: This study used quantitative proteomics to analyze milk fat globule membrane (MFGM) proteins in donkey colostrum (DC) and bovine colostrum (BC). A total of 179 (DC) and 195 (BC) MFGM proteins were identified, with 71 shared, 108 DC-specific, and 124 BC-specific proteins. Gene ontology analysis showed enrichment in cellular components and metabolic pathways, with 51 differentially expressed MFGM proteins identified.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Applied
Mohan Li, Haikun Yu, Jiali Chen, Rayhnigul Abdlla, Aicheng Liu, Wanying Song, Juan Zhang, Xiumin Zhang, Xiqing Yue, Qilong Li
Summary: This study characterized and compared whey proteins from donkey and bovine milk, identifying a large number of common and differentially expressed proteins. The analysis revealed molecular functions and metabolic pathways, shedding light on the differences between the two milk sources.
Article
Biochemistry & Molecular Biology
Mahmoud Abdel-Hamid, Pan Yang, Islam Mostafa, Ali Osman, Ehab Romeih, Yongxin Yang, Zizhen Huang, Awad A. Awad, Ling Li
Summary: Using labeled proteomics and bioinformatics tools, the study found differences between colostrum and mature milk from Mediterranean and Murrah buffaloes. Colostrum contains important proteins for immune system and muscle development, while mature milk has increased levels of lactoferrin for iron storage and hemoglobin formation.
Article
Food Science & Technology
Mohan Li, Qilong Li, Haikun Yu, Xiumin Zhang, Dehao Li, Wanying Song, Yan Zheng, Xiqing Yue
Summary: This study analyzed and compared the differentially expressed whey proteins (DEWPs) in donkey and bovine colostrum using proteomics. The results showed that these DEWPs were associated with cellular components, biological processes, and molecular functions, and were involved in various metabolic pathways. The findings provide valuable insights into the differences between donkey and bovine colostrum, as well as the important components for infant formula.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Review
Chemistry, Analytical
Jelena Radosavljevic, Dragana Stanic-Vucinic, Marija Stojadinovic, Mirjana Radomirovic, Ana Simovic, Milica Radibratovic, Tanja Cirkovic Velickovic
Summary: Whey protein, an important source of nutrition and biotechnology, is considered a by-product of the dairy industry due to low economic value. The challenge in whey protein separation lies in low concentration and similar properties. Ion exchange techniques are widely used for separation, and recent research on ion exchange membranes shows promise for industrial application. Adsorption-based methodologies targeting protein structure differences are anticipated to further improve whey protein separation.
CURRENT ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Boyuan Guan, Yang Gao, Yuxia Chai, Amantai Xiakouna, Xinping Chen, Xueyan Cao, Xiqing Yue
Summary: This study used site-specific glycoproteomics to investigate the N-glycosylation modifications of donkey whey protein. The results showed significant changes in site-specific N-glycans of donkey whey protein during lactation, providing new insights into the biological function of donkey whey protein.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Binsong Han, Lina Zhang, Peng Zhou
Summary: This study analyzed whey proteins in bovine, goat and camel milk using label-free proteomics techniques. A total of 840 proteins were identified, significantly increasing the number of whey proteins identified in these species. The study also revealed differences in whey proteome patterns between bovine, goat and camel milk, and identified specific proteins that act as markers for classification. Statistical analysis showed significant differences in the relative abundances of whey proteins among different species. GO and KEGG functional analysis indicated similar biological functions but varying protein quantities across species. These findings provide insights into the quantitative differences and potential physiological functions of whey proteins across species, and may have implications for the production of specific functional whey proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Mohan Li, Yushi Dong, Weixuan Li, Xinyu Shen, Rayhnigul Abdlla, Jiali Chen, Xueyan Cao, Xiqing Yue
Summary: This study conducted a qualitative proteomic analysis of donkey and bovine whey proteins, revealing significant differences in their composition and identifying unique whey proteins in each source. The analysis of protein functions and pathways provides a theoretical basis for the development of whey protein nutrition.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liqiang Han, Qixue Huang, JingNa Yang, Wenyan Lu, Mingyue Hu, Yanbin Yang, Heshui Zhu, Kun Pang, Guoyu Yang
Summary: Milk fat in mammary epithelial cells is synthesized and secreted as lipid droplets in the form of milk fat globules (MFGs) covered by a lipid-protein membrane called the milk fat globule membrane (MFGM). This study investigated the effects of MFGM proteins from control and conjugated linoleic acid (CLA)-treated milk on lipid droplet formation in MAC-T cells. Results showed that CLA reduced milk fat content and MFG size parameters, while increasing the specific surface area (SSA). Proteomic analysis identified differentially expressed proteins in the CLA group, and CLA-MFGM proteins promoted lipid droplet fusion and influenced droplet size distribution in MAC-T cells.
Article
Agriculture, Multidisciplinary
Changqing An, Ke Yang, Jieliang Zhu, Wenchuan Guo, Chang Lu, Xinhua Zhu
Summary: In this study, the identification of adulterated colostrum was achieved through dielectric spectroscopy analysis. Noise interference was found to be the main factor affecting the identification performance, while the scattering effect had a minor impact. Linear models showed higher accuracy rates.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Applied
Huiwen Zhao, Mohan Li, Qing Zhu, Aicheng Liu, Jiayang Bi, Zhizhong Quan, Xue Luo, Yan Zheng, Ning Yang, Xiqing Yue, Xueyan Cao
Summary: This study used label-free quantitative proteomics to analyze and compare milk fat globule membrane (MFGM) proteins in porcine colostrum and porcine mature milk. A total of 3917 and 3966 MFGM proteins were identified in colostrum and mature milk, respectively. Of these, 3807 proteins were common to both groups, with 303 proteins showing significant differential expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed the functions and pathways associated with the differentially expressed MFGM proteins. These findings provide crucial insights into the functional diversity of MFGM proteins in porcine milk and offer theoretical guidance for future research and development.
Article
Agriculture, Dairy & Animal Science
Ke Yang, Changqing An, Jieliang Zhu, Wenchuan Guo, Chang Lu, Xinhua Zhu
Summary: This study compared the performance of near-infrared spectroscopy (NIRS) and dielectric spectroscopy (DS) in quantitatively predicting the content of mature milk as an adulterant in bovine colostrum. The results showed that DS had better identification performance, providing important insights for the quantitative prediction of nonhomogeneous liquid food.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Manish Kumar Singh, Arvind Kumar, Ramadevi Nimmanapalli, Anand Kumar Pandey
Summary: In this study, electrophoresis and high-resolution mass spectrometry were used to identify various proteins in the whey proteome of Indian Jersey crossbreed bovines. The results showed that chromosomes 5 and 9 exhibited the highest protein expression. The major expressed proteins included glycosylation-dependent cell adhesion molecule-1, ubiquitin, desmoglein, annexin, glycoprotein, arginase, histones, peroxiredoxin, vimentin, desmin, catenin, peripherin, and 70 kDa heat shock protein.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)