Article
Biochemistry & Molecular Biology
Natasha S. Clayton, Millie Fox, Jose J. Vicente-Garcia, Courtney M. Schroeder, Trevor D. Littlewood, Jonathon Wilde, Kadalmani Krishnan, Murray J. B. Brown, Claire Crafter, Helen R. Mott, Darerca Owen
Summary: Activated Cdc42-associated kinase (ACK) phosphorylates PI3K regulatory subunits, promoting cell proliferation. ACK interacts with p85 alpha in nuclear-enriched cell fractions, facilitating dimerization of the regulatory subunits.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Meenakshi A. Chellaiah
Summary: In osteoclasts treated with TNF-alpha, the phosphorylation of L-PLastin can be attenuated by inhibitors of TNF-receptor 1, signaling proteins, and TRAF-6 siRNA. However, inhibitors of integrin alpha v beta 3, PKC, or PKA do not affect TNF-alpha-induced L-PLastin phosphorylation. Furthermore, Src and PI3-K are shown to regulate TRAF-6 phosphorylation, while Rho and Rho-kinase are downstream of TRAF-6 regulation.
Review
Cell Biology
Szabolcs Sipeki, Kitti Koprivanacz, Tamas Takacs, Anita Kurilla, Loretta Laszlo, Virag Vas, Laszlo Buday
Summary: Signal transduction is essential for cells to perceive and respond to information, with subcellular hubs formed via protein interactions playing a central role. The SH2 and SH3 domains serve as prototypes for interaction domains that recognize proteins, lipids, nucleic acids, and small molecules. The lipid-binding properties of SH2 and SH3 domains are also important for modulating complex assembly.
Review
Cell Biology
Mehrnaz Mehrabipour, Neda S. Kazemein Jasemi, Radovan Dvorsky, Mohammad R. Ahmadian
Summary: SRC homology 3 (SH3) domains are modular building blocks that facilitate the assembly of protein complexes through interactions with proline-rich/noncanonical motifs. They have been extensively studied across different life kingdoms and viruses, playing roles in various biological processes. Additionally, SH3 domains are implicated in the development of human diseases and are potential drug targets. This review summarizes the classification of SH3 domain functions, their binding specificity, and their significance in disease research.
Article
Genetics & Heredity
Pascale Lemieux, David Bradley, Alexandre K. Dube, Ugo Dionne, Christian R. Landry
Summary: Protein-protein interactions (PPIs) play crucial roles in cellular processes. This study investigates the evolution of SH3 domains in paralogous yeast type I myosins. The results show that the differences in SH3-dependent interactions between paralogs are not solely determined by the domains themselves. Ancestral sequence reconstruction reveals a gradual loss of interaction with modern partners for older ancestral domains, which is not caused by the affinity with the proline motifs. This suggests that SH3 domains and their host proteins may have essential intramolecular or allosteric interactions in SH3-dependent PPIs.
Article
Immunology
Pawel Borowicz, Vibeke Sundvold, Hanna Chan, Greger Abrahamsen, Hanna Kjelstrup, Tuula A. Nyman, Anne Spurkland
Summary: The study reveals that Lck Tyr(192) regulates Lck pTyr(394) and pTyr(505) activity, playing a crucial role in maintaining a pool of already active Lck in resting T cells. This provides an additional mechanism for fine-tuning Lck and T cell activity.
JOURNAL OF IMMUNOLOGY
(2021)
Review
Multidisciplinary Sciences
Raghuveera Kumar Goel, Nayoung Kim, Kiven Erique Lukong
Summary: SRMS is a non-receptor tyrosine kinase that was first discovered in a screen for genes regulating murine neural precursor cells in 1994. It lacks the regulatory tyrosine site that is critical for the enzymatic activity of SFKs. SRMS has a unique subcellular localization and its function is still relatively unknown. Recent studies have identified potential cellular substrates and highlighted its role in cancer.
Article
Veterinary Sciences
Ning Han, Ying Su, Mi Guo, Li Yu
Summary: Diabetic retinopathy is a common complication of diabetes that can lead to blindness. In this study, the role of protein tyrosine phosphatase 2 (SHP2) in diabetic retinopathy was investigated, and it was found that inhibiting SHP2 can alleviate retinal pathological changes and reduce inflammation and oxidative stress in rats with diabetic retinopathy.
EXPERIMENTAL ANIMALS
(2022)
Article
Medicine, General & Internal
Nevin Ersoy, Burcu Acikgoz, Ilkay Aksu, Amac Kiray, Husnu Alper Bagriyanik, Muge Kiray
Summary: The present study investigated the effects of 50 Hz, 3 mT electromagnetic field (EMF) exposure on testis development. The results showed that EMF exposure may cause deterioration in the structure and function of the testes, as well as affect the levels of growth factors. This suggests that EMF exposure during embryonic development and adolescence can lead to apoptosis and structural changes in the testes.
MEDICINA-LITHUANIA
(2023)