Article
Multidisciplinary Sciences
Miguel Angel Merlos Rodrigo, Hana Michalkova, Vladislav Strmiska, Berta Casar, Piero Crespo, Vivian de los Rios, J. Ignacio Casal, Yazan Haddad, Roman Guran, Tomas Eckschlager, Petra Pokorna, Zbynek Heger, Vojtech Adam
Summary: This study revealed that up-regulation of metallothionein-3 (hMT3) in neuroblastoma cells was associated with increased cisplatin resistance, indicating a potential cause for tumor relapse. Multiple biological pathways related to apoptosis, transport, proteasome, and cellular stress were implicated in cisplatin resistance in hMT3-overexpressing neuroblastoma cells.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Applied
Rocio Galindo-Lujan, Laura Pont, Zoran Minic, Maxim V. Berezovski, Victoria Sanz-Nebot, Fernando Benavente
Summary: This study analyzed the proteins from different varieties of quinoa seeds from Peru and Bolivia using advanced LC-MS/MS technology, identifying protein similarities and differences in the proteome. The comprehensive experimental quinoa seed proteome map presented in this study serves as a valuable starting point for further characterization and nutritional studies of quinoa and quinoa-containing food products.
Article
Medicine, Research & Experimental
Eman A. Abdelghffar, Wael A. Obaid, Zuhair M. Mohammed Saleh, Wafae Ouchari, Omayma A. Eldahshan, Mansour Sobeh
Summary: This study investigated the potential mitigative effects of Ajwa dates fruit extract (ADFE) on cisplatin-induced nephrotoxicity in rats and identified its components through phytochemical profiling. The results demonstrated that ADFE has significant antioxidant and anti-inflammatory properties, which can enhance the antioxidant status, reduce oxidative stress and inflammation, and potentially prevent and treat the side effects of chemotherapy.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Pharmacology & Pharmacy
Sepideh Mirzaei, Aliasghar Tabatabaei Mohammadi, Mohammad Hossein Gholami, Farid Hashemi, Ali Zarrabi, Amirhossein Zabolian, Kiavash Hushmandi, Pooyan Makvandi, Marek Samec, Alena Liskova, Peter Kubatka, Noushin Nabavi, Amir Reza Aref, Milad Ashrafizadeh, Haroon Khan, Masoud Najafi
Summary: Nrf2 signaling plays a crucial role in protecting against oxidative damage, but its activation in cancer cells can lead to chemoresistance. Modulating Nrf2 signaling during cisplatin treatment can suppress the development of chemoresistance and alleviate drug toxicity. Activating Nrf2 signaling can help prevent the side effects of cisplatin.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Yufeng Ma, Fengsen Zhang, Yijing Zhong, Yongchun Huang, Qiangqiang Yixizhuoma, Qiangqiang Jia, Shoude Zhang
Summary: Phosphodiesterase type 5 (PDE5) regulates the duration of cGMP signal by hydrolyzing cGMP to GMP. Inhibiting PDE5A has been effective in treating pulmonary arterial hypertension and erectile dysfunction. A new LC/MS-based enzymatic activity assay without labeling was developed, which quantifies cGMP and GMP to detect PDE5A activity. This method was verified with a fluorescently labeled substrate and a new PDE5A inhibitor with an IC50 value of 870 nM was identified. Overall, this strategy provides a new method for screening PDE5A inhibitors.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ranjith Kumar Bakku, Ravi Gupta, Cheol-Woo Min, Sun-Tae Kim, Genboku Takahashi, Junko Shibato, Seiji Shioda, Fumiko Takenoya, Ganesh Kumar Agrawal, Randeep Rakwal
Summary: This research employed high-throughput proteomics technique to investigate the tuber proteome of Jerusalem artichoke, a plant known for its medicinal properties. By extracting and analyzing proteins from commercially processed tuber products, the study provided a comprehensive protein list for Jerusalem artichoke tuber and discussed the associations between these proteins and health and disease regulatory metabolism.
Article
Biochemical Research Methods
Huu-Quang Nguyen, Dabin Lee, Yeoseon Kim, Geul Bang, Kun Cho, Young-Sun Lee, Jong Eun Yeon, David M. Lubman, Jeongkwon Kim
Summary: This study utilized LC-MS/MS to analyze the protein expression profiles of extracellular vesicles from serum samples of patients with various fatty liver diseases, revealing significant differences in protein expression levels of apolipoproteins, immunoglobulins, and other markers. Through principal component analysis, a clear differentiation between ALD and NAFLD was achieved. The up-regulation of alpha-2-macroglobulin in alcoholic hepatitis and apolipoprotein C3 in NAFLD were identified as significant findings.
JOURNAL OF PROTEOMICS
(2021)
Article
Oncology
Marie Belhajova, Natalia Podhorska, Ales Vicha, Tomas Eckschlager
Summary: This study found that the expression level of KDM5B is associated with cisplatin resistance in neuroblastoma cells. KDM5B silencing enhances proliferation and migration ability in cisplatin-resistant cells, and short interfering KDM5B transfection increases the S phase in resistant cells. Therefore, KDM5B may be involved in the survival mechanism of neuroblastoma cells.
Article
Biochemical Research Methods
Pauline Hiort, Christoph N. Schlaffner, Judith A. Steen, Bernhard Y. Renard, Hanno Steen
Summary: In liquid-chromatography-tandem-mass-spectrometry-based proteomics, we have developed a computational tool called multiFLEX-LF, which can quickly detect protein modifications and quantify their extent. It also allows for hierarchical clustering analysis to study modification dynamics and co-regulated modifications. This tool is capable of analyzing large data sets and driving the investigation of modification dynamics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Fei Xue, Bing Wang, Dong-xiao Guo, Yang Jiao, Wei-liang Cui, Xian-long Cheng, Zhi-bin Wang, Xue Yin, Shuang-cheng Ma, Yong-qiang Lin
Summary: Deer horn gelatin (DHG) is a valuable nutritional dietary supplement, but it is difficult to assess its quality and clarify the species of its raw material. This study used advanced analytical techniques to identify peptide markers specific to DHG and developed strategies for assessing its quality. The strategies can be used to evaluate the quality of deer gelatin.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Jie Hong, Shuchen Xin, Rui Min, Yongqian Zhang, Yulin Deng
Summary: The study proposed a peptide-based strategy to diagnose type 2 diabetes by quantifying hemoglobin tryptic peptides, identifying sensitive and insensitive peptides to glucose, and validating three promising biomarkers for diabetes diagnosis.
Article
Agriculture, Multidisciplinary
Barbara Prandi, Chiara Vacca, Stefano Sforza, Tullia Tedeschi
Summary: The increasing awareness of consumers on sustainable food chains has caused a shift in consumption from animal protein sources to plant protein sources. Soy, which is both relevant for human food use and animal feed, has a high protein content but is accompanied by antinutritional factors such as Kunitz's trypsin inhibitor (KTI). A direct label-free liquid chromatography-mass spectrometry (LC-MS) method has been developed to identify and quantify Kunitz inhibitor KTI3 in soybean and its derivative products, using a marker peptide specific to the protein of interest. The results of the LC-MS method were compared with spectrophotometric trypsin inhibition, demonstrating the complementarity of these two types of analysis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zheng Zhang, Qiangsheng Hu, Shuang Ye, Libing Xiang
Summary: This study reveals that PIN1 affects ferroptosis and sensitivity to DDP in CC cells via the NRF2/GPX4 axis, thereby identifying PIN1 as a potential therapeutic target for CC. The mechanism involves PIN1 silencing downregulating GPX4 by decreasing the level of NRF2. PIN1 also plays a role in the resistance to cisplatin-induced ferroptosis.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Plant Sciences
Muhammad Afzal, Malte Sielaff, Valentina Curella, Manjusha Neerukonda, Khaoula El Hassouni, Detlef Schuppan, Stefan Tenzer, C. Friedrich H. Longin
Summary: The study analyzed the expression of 756 proteins across 150 wheat cultivars using proteomics and found that environmental conditions have a significant impact on protein expression. Wheat cultivars varied greatly in their protein profiles, with 114 proteins being crucial for bread quality and human health.
Article
Chemistry, Analytical
Fazli Khuda, Zafar Iqbal, Ayub Khan, Zakiullah, Samiullah, Muhammad Umar Khayam Sahibzada, Mahboob Alam, Ameer Khusro
Summary: The study demonstrated that fresh pomegranate juice had no significant impact on the pharmacokinetic profile of artemether, including parameters such as AUC, C-max, elimination half-life, and time to reach peak plasma concentration.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Oncology
Elisabetta Valentini, Marta Di Martile, Donatella Del Bufalo, Simona D'Aguanno
Summary: Hypoxia, a characteristic of the tumor microenvironment, regulates multiple pathways and influences cancer progression and therapy resistance. Semaphorins and their receptors play a crucial role in the communication between cancer and stromal cells, affecting the development, progression, and therapy response of various cancer types, including melanoma. Further research is needed to understand the mechanisms by which semaphorins exert their effects and explore their potential as prognostic markers or therapeutic targets in melanoma and other malignancies.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Oncology
Simona D'Aguanno, Fabiana Mallone, Marco Marenco, Donatella Del Bufalo, Antonietta Moramarco
Summary: Hypoxia plays a crucial role in melanoma progression, affecting invasiveness, angiogenesis, and treatment response. Different subtypes of melanoma have distinct characteristics in biology, etiology, and clinical features. Understanding hypoxia-regulated pathways and utilizing novel antiangiogenic therapies may improve survival outcomes for melanoma patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Lorenzo Monteleone, Andrea Speciale, Giulia Elda Valenti, Nicola Traverso, Silvia Ravera, Ombretta Garbarino, Riccardo Leardi, Emanuele Farinini, Antonella Roveri, Fulvio Ursini, Claudia Cantoni, Maria Adelaide Pronzato, Umberto Maria Marinari, Barbara Marengo, Cinzia Domenicotti
Summary: Cancer stem cells (CSCs) play a crucial role in chemoresistance due to their high antioxidant defenses. This study found that combined treatment with Etoposide, Sulfasalazine, or an inhibitor of PKC alpha can sensitize CSCs to Etoposide by reducing their antioxidant defenses and inducing ferroptosis. The results suggest that PKC alpha inhibition inducing ferroptosis may be a promising strategy to combat CSC chemoresistance.
Article
Pharmacology & Pharmacy
Silvia Scaricamazza, Illari Salvatori, Susanna Amadio, Valentina Nesci, Alessio Torcinaro, Giacomo Giacovazzo, Aniello Primiano, Michela Gloriani, Niccolo Candelise, Luisa Pieroni, Jean-Philippe Loeffler, Frederique Rene, Cyril Quessada, Tesfaye W. Tefera, Hao Wang, Frederik J. Steyn, Shyuan T. Ngo, Gabriella Dobrowolny, Elisa Lepore, Andrea Urbani, Antonio Musaro, Cinzia Volonte, Elisabetta Ferraro, Roberto Coccurello, Cristiana Valle, Alberto Ferri
Summary: The therapeutic potential of the multi-target drug trimetazidine was evaluated in SOD1(G93A) mice. The results showed that trimetazidine delayed disease progression, improved motor function and metabolism, and increased overall survival of the mice.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Stefano Levi Mortera, Pamela Vernocchi, Ilaria Basadonne, Alessandro Zandona, Marco Chierici, Martina Durighello, Valeria Marzano, Simone Gardini, Antonio Gasbarrini, Andrea Urbani, Stefano Vicari, Paola Roncada, Cesare Furlanello, Paola Venuti, Lorenza Putignani
Summary: In recent years, studies have shown an increasing relationship between neurodevelopmental disorders and the microbial communities of the intestinal tract. This research utilized a novel metaproteomic approach to investigate the gut microbiota of children with Autism Spectrum Disorders (ASD) compared to relatives and age-matched controls, revealing distinctive functional differences.
JOURNAL OF PROTEOMICS
(2022)
Article
Medicine, General & Internal
Elena Di Pierro, Francesca Granata, Michele De Canio, Mariateresa Rossi, Andrea Ricci, Matteo Marcacci, Giacomo De Luca, Luisa Sarno, Luca Barbieri, Paolo Ventura, Giovanna Graziadei
Summary: Erythropoietic protoporphyria (EPP) and X-linked protoporphyria (XLP) are inherited disorders resulting from defects in enzymes of the heme biosynthetic pathway. They both lead to the systemic accumulation of protoporphyrin IX (PPIX) and cause cutaneous photosensitivity. This review provides an overview of established facts and latest findings on these rare diseases.
Article
Chemistry, Analytical
Mozghan Boroumand, Barbara Manconi, Simone Serrao, Federica Iavarone, Alessandra Olianas, Tiziana Cabras, Cristina Contini, Luisa Pieroni, Maria Teresa Sanna, Giovanni Vento, Chiara Tirone, Claudia Desiderio, Antonella Fiorita, Gavino Faa, Irene Messana, Massimo Castagnola
Summary: Glutathionylation and cysteinylation can protect critical cysteines from oxidative damages. Levels of S100A9 long and cystatin B in saliva were determined in preterm and at-term newborns, showing that the percentage of S-thiolated derivatives increased with postconceptional age, with the most significant variation occurring around 280 days. Differences in S-thiolated derivatives levels were found to be dependent on postconceptional age, not on preterm or at-term birth.
SEPARATION SCIENCE PLUS
(2022)
Article
Cell Biology
Christian Barbato, Paola Frisone, Laura Braccini, Simona D'Aguanno, Luisa Pieroni, Maria Teresa Ciotti, Caterina Catalanotto, Carlo Cogoni, Francesca Ruberti
Summary: RNA-binding proteins play important roles in miRNA-mediated gene silencing. This study discovered that SERBP1 is a partner protein of Argonaute2, and it participates in miR-92-mediated gene silencing by regulating the 3' UTR of KCC2. These findings advance our understanding of KCC2 translational regulation in neuronal cells and highlight the key role of SERBP1 in this gene regulation.
Article
Cell Biology
Patrizia Bottoni, Alessandro Pontoglio, Salvatore Scara, Luisa Pieroni, Andrea Urbani, Roberto Scatena
Summary: Mitochondrial bioenergetics play an important role in pathophysiology, especially as unintended targets of different drugs. PPAR ligands, a class of drugs that activate Peroxisome Proliferator-Activated Receptors, were found to inhibit NADH coenzyme Q reductase and induce complex mitochondrial oxidative metabolism damage. This damage affects not only ATP synthesis but also respiratory control ratio, spare respiratory capacity, coupling efficiency, oxidative stress, and mitochondrial structural damage.
Article
Health Care Sciences & Services
Cristina Graziani, Lucrezia Laterza, Claudia Talocco, Marco Pizzoferrato, Nicoletta Di Simone, Silvia D'Ippolito, Caterina Ricci, Jacopo Gervasoni, Silvia Persichilli, Federica Del Chierico, Valeria Marzano, Stefano Levi Mortera, Aniello Primiano, Andrea Poscia, Francesca Romana Ponziani, Lorenza Putignani, Andrea Urbani, Valentina Petito, Federica Di Vincenzo, Letizia Masi, Loris Riccardo Lopetuso, Giovanni Cammarota, Daniela Romualdi, Antonio Lanzone, Antonio Gasbarrini, Franco Scaldaferri
Summary: Recurrent cystitis (RC) is a common disease mainly caused by intestinal microbiota, with the alteration in intestinal permeability and reduced diversity of gut microbiota playing a major role in its pathogenesis.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Stefano Levi Mortera, Valeria Marzano, Pamela Vernocchi, Maria Cristina Matteoli, Valerio Guarrasi, Simone Gardini, Federica Del Chierico, Novella Rapini, Annalisa Deodati, Alessandra Fierabracci, Stefano Cianfarani, Lorenza Putignani
Summary: Recent research has shown a correlation between the composition of the human gut microbiota and the onset of type 1 diabetes. In this study, a comprehensive metaproteomic investigation was conducted on children with type 1 diabetes, revealing potential gut microbiota features related to the severity of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Antonella Roveri, Flavio Di Giacinto, Monica Rossetto, Giorgio Cozza, Qing Cheng, Giovanni Miotto, Lucio Zennaro, Maria Luisa Di Paolo, Elias S. J. Arner, Marco De Spirito, Matilde Maiorino, Fulvio Ursini
Summary: The aim of this study was to investigate the functional role of the Arg152 residue in GPX4, and its mutation to His in SSMD. The mutation did not affect the catalytic mechanism of the enzyme, but showed a different response to the concentration of enzyme and membrane cardiolipin compared to the wild-type enzyme. The findings suggest that GPX4 in cardiolipin containing mitochondria is unique and may be involved in the pathology of SSMD.
Article
Biochemistry & Molecular Biology
Federica Del Chierico, Giorgia Conta, Maria Cristina Matteoli, Alessandra Fierabracci, Sofia Reddel, Gabriele Macari, Simone Gardini, Valerio Guarrasi, Stefano Levi Mortera, Valeria Marzano, Pamela Vernocchi, Fabio Sciubba, Federico Marini, Annalisa Deodati, Novella Rapini, Stefano Cianfarani, Alfredo Miccheli, Lorenza Putignani
Summary: Specific gut microbiota profiles were identified in T1D patients and their relatives, which differed from healthy subjects. These specific microbial and metabolic profiles may be associated with the progression and severity of T1D.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Elisabetta Valentini, Simona D'Aguanno, Marta Di Martile, Camilla Montesano, Virginia Ferraresi, Alexandros Patsilinakos, Manuela Sabatino, Lorenzo Antonini, Martina Chiacchiarini, Sergio Valente, Antonello Mai, Gianni Colotti, Rino Ragno, Daniela Trisciuoglio, Donatella Del Bufalo
Summary: Bcl-2 family anti-apoptotic proteins play a significant role in cancer, and finding new molecules targeting these proteins has therapeutic potential. Through virtual screening and experimental validation, IS20 and IS21 were identified as compounds that can inhibit the activity of Bcl-2 family proteins and have anti-proliferative and pro-apoptotic effects on various tumors.