期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 10, 页码 4567-4578出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr2004117
关键词
tamoxifen; anterior gradient 2 homologue; AGR2; PAcIFIC; data independent
资金
- USA NIH Pharmaceutical Sciences [T32 GM07750]
- USA NIH NCRR [1S10RR017262-01]
- University of Washington's Proteomics Resource [UWPR95794]
- Cancer Research UK [C483/A6354]
- BBSRC (UK) [BB/C511599/1]
- Biotechnology and Biological Sciences Research Council [BB/C511599/1] Funding Source: researchfish
A label-free quantitative variation of the recently developed data-independent shotgun proteomic method precursor acquisition independent from ion count (PAcIFIC) was used to identify novel proteins implicated in cancer progression and resistance. Specifically, this screen identified the pro-metastatic protein anterior gradient 2 (AGR2) as significantly up-regulated in tamoxifen-treated cells. Highlighting the need for direct proteome profiling methods like PAcIFIC, neither data-dependent gas-phase fractionation nor a transcriptomic screen detected AGR2 protein/transcript at significantly up-regulated levels. Further cell-based experiments using human cancer cell lines and in vivo xenografts confirmed the PAcIFIC hypothesis that AGR2 is up-regulated in MCF-7 cells post tamoxifen treatment and that it is implicated in drug resistance mediation.
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