Article
Oncology
John C. Dawson, Alison Munro, Kenneth Macleod, Morwenna Muir, Paul Timpson, Robert J. Williams, Margaret Frame, Valerie G. Brunton, Neil O. Carragher
Summary: Studied the mechanism of action of SRC inhibitor AZD0424 in cancer cells, confirming its ability to inhibit cell viability and reduce cell signaling pathway activation, especially with synergistic effects when used in combination with MEK inhibitors. Additionally, demonstrated that SRC inhibition is an effective way to block resistance to MEK inhibitors.
MOLECULAR ONCOLOGY
(2022)
Article
Nutrition & Dietetics
Katherine M. Weh, Yun Zhang, Connor L. Howard, Amy B. Howell, Jennifer L. Clarke, Laura A. Kresty
Summary: This study investigated the inhibitory effects of cranberry polyphenols on premalignant Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC) cell lines. The results showed that cranberry polyphenols, particularly C-PAC, were more effective at inducing cell death in these cell lines compared to a combination of anthocyanins, flavonoids, and glycosides (AFG). The analysis of protein pathways revealed previously unidentified mechanisms by which cranberry polyphenols exert their cancer inhibitory effects. These findings provide new insights into the potential use of cranberry constituents as preventive agents for EAC.
Article
Cell Biology
Irina Gromova, Jaime A. Espinoza, Morten Grauslund, Eric Santoni-Rugiu, Maj-Lis Moller Talman, Jan van Oostrum, Jose M. A. Moreira
Summary: Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by the absence of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression, often requiring conventional chemotherapy for treatment. Analysis of protein activation state revealed heterogenous pathway activation, with PI3 K/AKT/mTOR signaling being the most common event. Individualized therapeutic possibilities, such as targeting oncogenic KIT in association with specific protein markers, may have clinical value.
Article
Clinical Neurology
Md. Mahiuddin Ahmed, Andrew J. Carrel, Yasmin Cruz Del Angel, Jessica Carlsen, Ajay X. Thomas, Marco I. Gonzalez, Katheleen J. Gardiner, Amy Brooks-Kayal
Summary: The study utilized Reverse Phase Protein Arrays (RPPA) to profile changes in protein expression in a pilocarpine mouse model of epilepsy. Results revealed time-dependent alterations in multiple components of major signaling pathways and cellular complexes during epileptogenesis, as well as specific changes in correlations among functionally related proteins in different brain regions over time.
FRONTIERS IN NEUROLOGY
(2021)
Article
Neurosciences
Meng Jin, Ruidie Shi, Daili Gao, Baokun Wang, Ning Li, Xia Li, Attila Sik, Kechun Liu, Xiujun Zhang
Summary: This study aims to identify a promising biomarker for Parkinson's disease (PD) using reverse phase protein array (RPPA) technology and in vivo verification. The biomarker discovered can facilitate early intervention in PD, leading to improved treatment effectiveness and reduced disease morbidity.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Nobuhiko Asakura, Naotoshi Nakamura, Atsushi Muroi, Yosui Nojima, Taro Yamashita, Shuichi Kaneko, Kazuki Ikeda, Naohiko Koshikawa, Takashi Suzuki
Summary: This study investigated the heterogeneity of 17 liver cancer cell lines and found distinct patterns of protein expression and phosphorylation in the EGFR and EphA2 signaling pathways between CD90-positive and EpCAM-positive cells. The activation of AKT was identified as a key pro-oncogenic regulator in HCC, suggesting its potential role in targeted therapies for hepatocellular carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Gunhee Lee, Yeun-Jun Chung, Minho Lee
Summary: The study developed models to predict protein levels using transcriptome and reverse phase protein arrays. By utilizing a 47-gene expression panel, the prediction performance was enhanced, doubling the number of predictable protein expressions. However, the model had limitations due to the weaknesses of RPPA.
CURRENT BIOINFORMATICS
(2021)
Article
Multidisciplinary Sciences
Chao Chen, Zhenxing Zhang, Caiyun Liu, Bin Wang, Ping Liu, Shu Fang, Fan Yang, Yongping You, Xinjian Li
Summary: Excessive consumption of fructose contributes to cancer development. This study reveals that glucose deprivation induces fructolysis in glioblastoma cells, which is mediated by ATF4. Inhibition of ATF4-dependent fructolysis significantly suppresses growth and colony formation of glioblastoma cells in vitro and in vivo.
NATURE COMMUNICATIONS
(2022)
Article
Health Care Sciences & Services
Nupur Mukherjee, Alacoque Browne, Laura Ivers, Tapesh Santra, Mattia Cremona, Bryan T. Hennessy, Norma O'Donovan, John Crown, Walter Kolch, Dirk Fey, Alex J. Eustace
Summary: This study investigated the mechanism of resistance to cisplatin in triple negative breast cancer (TNBC) using a systems biology approach. The activation of the PI3K/AKT signaling pathway was found to be closely associated with cisplatin resistance. Experimental validation confirmed the sensitivity of TNBC cell lines with activated PI3K/AKT signaling to combination therapy of cisplatin and PI3K/AKT pathway inhibitors.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Geoffrey Shapiro, Patricia LoRusso, Daniel C. Cho, Luna Musib, Yibing Yan, Matthew Wongchenko, Ilsung Chang, Premal Patel, Iris T. Chan, Sandra Sanabria-Bohorquez, Raymond D. Meng, Johanna C. Bendell
Summary: The study explored the combination dosing of cobimetinib and ipatasertib in patients with advanced solid tumors. Although limited tolerability and efficacy were observed, some patients experienced partial response, especially those with PTEN-low disease. Pharmacodynamic analyses indicated target engagement and suggested further exploration of these agents in combination with other anticancer drugs.
INVESTIGATIONAL NEW DRUGS
(2021)
Article
Geriatrics & Gerontology
Matthew B. Friese, Taranjit S. Gujral, Arvind Palanisamy, Brittany Hemmer, Deborah J. Culley, Gregory Crosby
Summary: The study found that anesthetic agents may impact neural circuit development by inducing a shared signaling pathway, leading to changes in synaptic strength and number, widespread apoptosis, and long-lasting cognitive impairment.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jinjun Wang, Xinghua Guo
Summary: The study identified 4 RPPA subtypes in patients with LGG and found associations between these subtypes and specific magnetic resonance imaging features. The RPPA3 subtype was associated with the shortest overall survival and could be predicted preoperatively using radiogenomics analysis. This suggests that tailored management strategies based on RPPA subtype prediction may be clinically valuable in patients with LGG.
JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY
(2021)
Article
Oncology
Allison L. Hunt, Aratara Nutcharoen, Jamie Randall, Alyssa Papazian, John Deeken, G. Larry Maxwell, Nicholas W. Bateman, Emanuel F. Petricoin, Amin Benyounes, Thomas P. Conrads, Timothy L. Cannon
Summary: This case report underscores the importance of real-time integration of proteogenomic data from laser microdissected tumor specimens and dynamic biopsy testing in a molecular tumor board setting for improved precision oncology.
Article
Multidisciplinary Sciences
Qiang Lu, GenTu Ge, DuoWen Sa, ZhiJun Wang, MeiLing Hou, Yu Shan Jia
Summary: This study examined the changes in plant nutrients and microbial communities of alfalfa under salt stress. It found that varying degrees of salt stress influenced the growth and development of plants, as well as affected the nutritional quality of the plants and the structure of microbial communities in the surrounding soil.
Article
Plant Sciences
Millicent R. Smith, Eric Dinglasan, Erik Veneklaas, Jose Polania, Idupulapati M. Rao, Stephen E. Beebe, Andrew Merchant
Summary: This study investigated the physiological and chemical responses of 12 bush bean genotypes to drought and low phosphorus stress. The results showed that both drought and low phosphorus individually or combined led to significant decreases in seed weight and aboveground biomass, as well as reduced photosynthesis and stomatal conductance. Two bean genotypes exhibited superior adaptation to the combined stress conditions. Additionally, the study found that carbon isotope fractionation decreased in response to water deficit treatment and that key nutrients and amino acids showed significant changes in the soluble leaf fraction under combined water deficit and low phosphorus stress.
FRONTIERS IN PLANT SCIENCE
(2022)