Article
Agriculture, Dairy & Animal Science
A. Bouquet, V. Deru, B. Blanchet, P. Ganier, L. Flatres-Grall, B. Ligonesche, C. Carillier-Jacquin, E. Labussiere, H. Gilbert
Summary: This study found genetic relationships between digestive efficiency and sow reproductive traits, suggesting important implications for research and breeding purposes.
Article
Biochemical Research Methods
Daniel Perez Hernandez, Gunnar Dittmar
Summary: The analysis of protein-protein interactions is crucial for understanding cellular signaling. Peptide pull-downs, using chemically synthesized peptides, combined with mass spectrometry, allow for direct measurement of interactome changes induced by post-translational modifications or amino acid exchanges. Peptides are particularly useful for studying SLiM-mediated protein interactions, leading to the development of new technologies for interaction studies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Eduardo Esteves, Vera M. Mendes, Bruno Manadas, Rafaela Lopes, Liliana Bernardino, Maria Jose Correia, Marlene Barros, Ana Cristina Esteves, Nuno Rosa
Summary: COVID-19 is the most impacting global pandemic ever, and this study reveals new insights into the disease by analyzing the salivary proteome and identifying previously unknown proteins associated with SARS-CoV-2 infection, as well as uncovering the virus's influence on the host's immune response and energy metabolism.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Pan Zhang, Yu Fu, Ran Zhang, Peng Shang, Hao Zhang, Bo Zhang
Summary: The study found that KCTD15 plays an important role in pig fat deposition, with differential expression levels in different pig breeds. Additionally, the T-2030C site was identified as a crucial regulatory site for transcriptional activity in pig fat deposition.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Macarena Egea, Irene Penaranda, Maria Dolores Garrido, Maria Belen Linares, Cristian Jesus Sanchez, Josefa Madrid, Juan Orengo, Fuensanta Hernandez, Maria Arantzazu Aguinaga Casanas, Alberto Banos, Belen Barrero Dominguez, Silvia Lopez Feria, Silvia Martinez Miro
Summary: The experiment showed that adding Allium spp. extract and olive pulp in pork production has advantages, including improving meat and fat quality, enhancing water holding capacity, and altering fatty acid profile. Sensory analysis also indicated that meat samples from these treatments were more tender.
Article
Veterinary Sciences
Xinglong Gong, Min Zheng, Jian Zhang, Yourong Ye, Mengqi Duan, Yangzom Chamba, Zhongbin Wang, Peng Shang
Summary: Fat deposition traits play a crucial role in pig production and breeding. This study analyzed the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York pigs at different growth stages and identified key genes involved in regulating fat deposition in pigs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ximing Liu, Ying Bai, Ran Cui, Shuaihan He, Xingbo Zhao, Keliang Wu, Meiying Fang
Summary: This study found that testosterone can regulate fat deposition by regulating the expression of circRNA. The host genes of differentially expressed circRNA in backfat were associated with fatty acid transport, while in abdominal tissues, they were mainly involved in adipogenesis and inflammation. The interaction among sus_circPAPPA2, ssc-miR-2366, and GK was verified, and it was discovered that testosterone can inhibit preadipocyte differentiation by upregulating the expression of sus_circPAPPA2.
Review
Biochemistry & Molecular Biology
Ilya Kurbatov, Georgii Dolgalev, Viktoriia Arzumanian, Olga Kiseleva, Ekaterina Poverennaya
Summary: Increasing attention has been given to the study of protein-metabolite interactions, which are crucial for regulating protein functions and cellular processes. However, detecting and identifying these interactions is challenging due to their short-lived nature and limitations in current detection assays. Despite advances in mass spectrometry, there is still a need to improve the ability to identify and quantify protein-metabolite interactions for a comprehensive understanding of biological systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Katarzyna Piorkowska, Julia Sroka, Kacper Zukowski, Karolina Zygmunt, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: This study evaluated the effects of BSCL2 variants on slaughter and fattening characteristics and meat quality traits. The selection of mutations was based on variant calling analysis and chi test results using subcutaneous fat RNA-seq data. Significant genotype/allele distribution variations between high- and low-fat pigs were observed. The study suggests that variant calling from RNA-seq data may help develop genetic markers for complex pig traits, and the BSCL2 G allele (rs341493267) was found to increase backfat thickness in pigs.
Article
Food Science & Technology
Jingjing Li, Zhiqiang Li, Jinshan Ran, Chaowu Yang, Zhongzhen Lin, Yiping Liu
Summary: This study used liquid chromatography-mass spectrometry to differentiate between chicken meat and abdominal fat from different sources. Specific lipid markers were identified for different chicken breeds. A total of 209 lipid molecules were determined as marker candidates for abdominal fat and intramuscular fat authentication, with glycerophospholipids being the dominant class. Sphingolipids and PUFA-enriched glycerophospholipids were found in higher abundance in intramuscular fat, contributing to its unique flavor and nutritional value.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Veterinary Sciences
Aiyou Wen, Wei Zhang, Jingen Xu, Kunping Wang, Hong Hu
Summary: The study found that eccDNAs and ecDNAs are present in both Wei pig (WP) and Large White pig (LP), and they both have sizes large enough to carry one or several partial or complete genes. These findings have expanded our understanding of circular DNA in pigs and will serve as a reference for using pigs as a medical model and discovering new genetic markers for selecting high-quality breeds.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Genetics & Heredity
Yibing Liu, Ying Yu, Hong Ao, Fengxia Zhang, Xitong Zhao, Huatao Liu, Yong Shi, Kai Xing, Chuduan Wang
Summary: This study identified various lncRNAs in pigs that play a role in fat deposition and metabolism through RNA-seq. Further analysis revealed that some lncRNAs can affect swine backfat deposition by regulating target genes.
Article
Microbiology
Peng Shang, Mingbang Wei, Mengqi Duan, Feifei Yan, Yangzom Chamba
Summary: This study found that the composition and abundance of colonic intestinal microflora in Tibetan pigs were closely related to their superior traits. Bifidobacteria, Ruminococcaceae, and Family-XIII-AD3011-Group are conducive to improving disease resistance in Tibetan pigs, while Lactobacillus and Solobacterium are the main bacterial communities involved in fat deposition in Tibetan pigs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenxia Li, Yufen Chen, Yunting Zhang, Ning Zhao, Wanfeng Zhang, Mingyue Shi, Yan Zhao, Chunbo Cai, Chang Lu, Pengfei Gao, Xiaohong Guo, Bugao Li, Sung-Woo Kim, Yang Yang, Guoqing Cao
Summary: Pigs, especially the Mashen and Large White breeds, exhibit diverse transcriptional responses in skeletal muscle under cold stress. The Mashen pigs demonstrate stable physiological homeostasis and a strong immune response, while the Large White pigs primarily regulate heat production and energy metabolism. The PRSS8 gene plays a role in cellular energy metabolism and thermogenesis. These findings provide valuable insights into the molecular mechanisms underlying cold adaptation in pigs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jiayi Chen, Fengming Chen, Xue Lin, Yaodong Wang, Jianhua He, Yurong Zhao
Summary: A diet supplemented with excessive energy promoted IMF deposition by positively changing lipogenic potential while decreasing tenderness by increasing glycolytic muscle fibers, which together affected meat quality. In terms of growth performance and meat quality, the present study suggests that the low-energy diet is suitable for finishing Ningxiang pigs.
Article
Pediatrics
Timothy Vigers, Carissa Vinovskis, Lu-Ping Li, Pottumarthi Prasad, Hiddo Heerspink, Angelo D'Alessandro, Julie A. Reisz, Federica Piani, David Z. Cherney, Daniel H. van Raalte, Kristen J. Nadeau, Meda E. Pavkov, Robert G. Nelson, Laura Pyle, Petter Bjornstad
Summary: This study compared plasma metabolites between youth with and without type 1 diabetes mellitus (T1DM) and found lower levels of metabolites associated with amino acid oxidation and the tricarboxylic acid cycle in individuals with T1DM. These metabolites were related to kidney function and may serve as potential biomarkers for improving kidney bioenergetics.
PEDIATRIC NEPHROLOGY
(2023)
Article
Hematology
Ariel Hay, Travis Nemkov, Fabia Gamboni, Monika Dzieciatkowska, Alicia Key, Matthew Galbraith, Kyle Bartsch, Kaiqi Sun, Yang Xia, Mars Stone, Michael P. Busch, Philip J. Norris, James C. Zimring, Angelo D'Alessandro
Summary: Blood storage leads to the decrease of ATP and DPG in red blood cells, which play crucial roles in erythrocyte physiology, oxygen kinetics, and posttransfusion survival. Supplementing S1P to stored RBC units can improve energy metabolism and posttransfusion recovery.
Article
Cell Biology
Syed A. Mian, Celine Philippe, Eleni Maniati, Pantelitsa Protopapa, Tiffany Bergot, Marion Piganeau, Travis Nemkov, Doriana Di Bella, Valle Morales, Andrew J. Finch, Angelo D'Alessandro, Katiuscia Bianchi, Jun Wang, Paolo Gallipoli, Shahram Kordasti, Anne Sophie Kubasch, Michael Cross, Uwe Platzbecker, Daniel H. Wiseman, Dominique Bonnet, Delphine G. Bernard, John G. Gribben, Kevin Rouault-Pierre
Summary: Patients with myelodysplastic syndrome and ring sideroblasts (MDS-RS) suffer from symptomatic anemia due to ineffective erythropoiesis caused by SF3B1 mutations. Mis-splicing of COASY induced by these mutations affects heme biosynthesis and erythropoiesis. Supplementation with COASY substrate may serve as a potential treatment for anemia in MDS-RS patients.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Sport Sciences
Travis Nemkov, Francesca Cendali, Davide Stefanoni, Janel L. Martinez, Kirk C. Hansen, Inigo San-Millan, Angelo D'Alessandro
Summary: This study aimed to explore the metabolic characteristics of elite athletes before and after exercise in a World Tour cycling race through metabolomics analysis. The results showed significant differences in blood metabolites between aerobic and anaerobic exercise. Additionally, similar metabolic features were observed during climbing and sprinting stages in the World Tour race, and an increase in fatty acid oxidation capacity was correlated with competitive performance.
Article
Hematology
Brian Custer, Evan M. Bloch, Barbara J. Bryant, Angelo D'Alessandro, Meghan Delaney, Ruchika Goel, Eldad A. Hod, Cassandra D. Josephson, Louis M. Katz, Yvette M. Miller, Merlyn H. Sayers, Jansen N. Seheult, Darrell J. Triulzi, James Berger, Shimian Zou, Benyam Hailu, Simone A. Glynn, Nareg H. Roubinian
Summary: The National Heart, Lung, and Blood Institute and the Office of the Assistant Secretary for Health held a virtual symposium on transfusion medicine to discuss and define important unanswered scientific questions, and to identify research priorities in transfusion medicine.
Article
Pharmacology & Pharmacy
Hui Zhang, Angelo D'Alessandro, Min Li, Julie A. Reisz, Suzette Riddle, Akshay Muralidhar, Todd Bull, Lan Zhao, Evgenia Gerasimovskaya, Kurt R. Stenmark
Summary: This study investigates the effects of sildenafil on fibroblasts derived from patients with pulmonary hypertension (PH). Sildenafil showed specific effects on purine metabolites, especially adenosine, adenine, and xanthine. However, its impact on cell stress markers was limited. Combination treatment of sildenafil with HDAC inhibitors showed promising inhibitory effects on proliferation and metabolic reprogramming in PH-fibroblasts.
VASCULAR PHARMACOLOGY
(2023)
Article
Toxicology
Andrew A. Monte, Alexis Vest, Julie Haines, Danielle Berninzoni, Claire Hart, Layne Dylla, Angelo D'Alessandro, Kennon J. Heard, Cheyret Wood, Jack Pattee
Summary: This study used metabolomic and genomic data to predict liver injury and regeneration in patients taking therapeutic doses of acetaminophen. Several metabolites were found to be under genetic control and predictive of ALT elevation due to acetaminophen. These findings confirm the importance of mitochondrial energy production and the urea cycle in acetaminophen-induced liver injury.
JOURNAL OF MEDICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Ziya Isiksacan, Angelo D'Alessandro, Susan M. Wolf, David H. McKenna, Shannon N. Tessier, Erdem Kucukal, A. Aslihan Gokaltun, Nishaka William, Rebecca D. Sandlin, John Bischof, Narla Mohandas, Michael P. Busch, Caglar Elbuken, Umut A. Gurkan, Mehmet Toner, Jason P. Acker, Martin L. Yarmush, O. Berk Usta
Summary: Transfusion of red blood cells is a valuable and widespread treatment in modern medicine. Current practices in blood transfusion overlook the variability in stored red blood cell quality. It is necessary to rethink transfusion medicine workflows and utilize novel technologies for assessing red blood cell quality.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Hematology
Ian S. LaCroix, Mitchell Cohen, Ernest E. Moore, Monika Dzieciatkowska, Christopher C. Silliman, Kirk C. Hansen, Angelo D'Alessandro
Summary: This study characterized the multi-omics features of platelet transfusion efficacy in a clinically relevant cohort of trauma patients using metabolomics and proteomics approaches. The results showed higher levels of tranexamic acid, inflammatory proteins, carnitines, and polyamines in patients requiring platelet transfusion. The study also identified increased platelet activation in the most severely injured patients. This study provides the first multi-omics characterization of platelet transfusion efficacy.
Article
Biochemistry & Molecular Biology
Julie A. Reisz, Monika Dzieciatkowska, Daniel Stephenson, Fabia Gamboni, D. Holmes Morton, Angelo D'Alessandro
Summary: Lesch-Nyhan syndrome is a genetic metabolic disorder that causes intellectual deficits, neural abnormalities, and gout. A multi-omics analysis of red blood cells from patients with LN revealed various abnormalities in metabolism, protein expression, and mineral levels. These findings provide new insights for potential therapies for LN.
Review
Biochemistry & Molecular Biology
Angelo D'Alessandro, Alkmini T. Anastasiadi, Vassilis L. Tzounakas, Travis Nemkov, Julie A. Reisz, Anastsios G. Kriebardis, James C. Zimring, Steven L. Spitalnik, Michael P. Busch
Summary: Red blood cells play a central role in oxygen transport in the human body. Recent advancements in omics technologies have revealed the complex proteome and metabolome of red blood cells, leading to a reevaluation of the mechanisms underlying RBC metabolism. These findings have also provided insights into changes that occur during refrigerated storage of RBCs in blood banks, which are crucial for life-saving transfusions worldwide.
Article
Hematology
Ariel Hay, Karolina Dziewulska, Fabia Gamboni, David Nerguizian, Monika Dzieciatkowska, James C. Zimring, Angelo D'Alessandro
Summary: Hypoxic storage has been shown to improve post-transfusion recovery and energy metabolism in stored red blood cells in mice, providing a pre-clinical platform for further study.
Review
Hematology
Angelo D'Alessandro
Summary: With the advancement of high-throughput omics technologies, transfusion medicine has been able to explore the biology of blood donors, stored blood products, and transfusion recipients. These approaches have revealed the impact of genetic and non-genetic factors on the quality of stored blood products and efficacy of transfusion events. In the near future, precision transfusion medicine arrays and metabolomics will enable the development and implementation of machine learning strategies for personalized transfusion medicine.
TRANSFUSION MEDICINE AND HEMOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Alicia Key, Zachary Haiman, Bernhard O. Palsson, Angelo D'Alessandro
Summary: Red blood cells are an abundant and relatively simple type of cells in the human body. They have been used as an ideal model for studying metabolism due to their accessibility. Computational models have the potential to enhance our understanding of red blood cell metabolism and predict their metabolic behaviors. Metabolomics and systems biology have provided evidence to advance our understanding of the storage lesion in red blood cells.
Article
Chemistry, Analytical
Francesca Cendali, Angelo D'Alessandro, Travis Nemkov
Summary: Metabolomics and lipidomics techniques can comprehensively measure small molecules, and advanced microsampling devices offer a convenient way to monitor metabolomic and lipidomic changes during exercise. In this study, significant changes in energy and redox metabolism were observed in both male and female volunteers, while lipid changes were more individualized. This approach showcases the ability to monitor circulating metabolome and lipidome profiles in response to exercise.
ANALYTICAL SCIENCE ADVANCES
(2023)
