Article
Biochemical Research Methods
Dirk F. Moore, David E. Sleat, Peter Lobel
Summary: Knowledge of cellular location is crucial for understanding protein function. Subcellular fractionation followed by mass spectrometry is a commonly used method for assigning cellular locations to proteins. However, proteins can reside in multiple compartments, which requires a more accurate method for localization. In this article, the constrained proportional assignment (CPA) method is introduced, along with data transformations and R-based programs for analyzing subcellular proteomics data.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Petko Mladenov, Diana Zasheva, Sebastien Planchon, Celine C. Leclercq, Denis Falconet, Lucas Moyet, Sabine Brugiere, Daniela Moyankova, Magdalena Tchorbadjieva, Myriam Ferro, Norbert Rolland, Jenny Renaut, Dimitar Djilianov, Xin Deng
Summary: Desiccation-tolerant species provide important models for studying drought tolerance and improving crop resistance to drought. This study used various analytical methods to investigate the response of the resurrection plant H. rhodopensis to desiccation, revealing valuable insights and potential targets for further research on drought tolerance mechanisms and genetic engineering in crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qi Guo, Lei Liu, Won C. Yim, John C. Cushman, Bronwyn J. Barkla
Summary: The study utilized Free Flow Electrophoresis for high-resolution membrane fractionation of plant leaf tissue, combined with mass spectrometry-based proteomics, allowing simultaneous profiling of multiple cellular membranes. Comparison of protein profiles of fractionated membranes with known cellular compartment markers revealed protein functions and provided new evidence for multiple subcellular localizations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Xin Wang, Setsuko Komatsu
Summary: Climate change poses a threat to soybean production through reduced seed yield and quality. This review summarizes the morphophysiological changes in soybeans under abiotic stress and discusses cellular metabolisms and regulatory mechanisms in response to organellar stress using subcellular proteomics. The communication pathways involving reactive oxygen species scavenging, molecular chaperones, and phytohormone signals among subcellular compartments are highlighted. Given the complexity of climate change and plant limitations in coping with multiple abiotic stresses, a generic response involving calcium and abscisic acid signals in subcellular organelles is proposed. This review provides an overview of subcellular proteomics research on stressed soybeans and discusses the future potential of subcellular proteomics in enhancing climate-tolerant crops.
Article
Microbiology
Tobias Kroniger, Mina Mehanny, Rabea Schluter, Anke Trautwein-Schult, Bernd Kollner, Dorte Becher
Summary: We analyzed the proteomic response of the fish pathogen A. salmonicida to iron limitation, elevated incubation temperature, and antibiotic treatment. Several iron-regulated proteins were identified, some of which were not previously reported. We also observed increased abundance of certain proteins under both iron limitation and elevated temperature, suggesting their involvement in the infection process. Analysis of outer membrane vesicles revealed significant differences in proteomes, indicating their potential role in iron acquisition. The presence of iron-regulated transporters suggests that OMVs from stressed bacteria could be suitable vaccine candidates for inducing anti-virulence immune responses.
Article
Biodiversity Conservation
Alba Otero-Farina, Julie Retif, Isabelle Metais, Laurence Poirier, Amelie Chatel
Summary: Subcellular fractionation is a valuable technique for studying metal cell compartmentalization and evaluating the impact of contaminants in organisms. It provides important insights into the behavior of metals within cell compartments and their detoxification processes. This technique is particularly useful for studying the exposure and trophic transfer of metals in coastal and marine environments, using species like bivalves and fish as bioindicators. However, further research is needed to enhance the robustness of using subcellular fractionation for risk assessment.
ECOLOGICAL INDICATORS
(2022)
Article
Biology
Benjamin D. Hobson, Se Joon Choi, Eugene Mosharov, Rajesh K. Soni, David Sulzer, Peter A. Sims
Summary: By employing APEX2 proximity labeling and mass spectrometry, we have successfully mapped the subcellular proteomes of midbrain dopaminergic neurons and identified key proteins involved in proteostasis, metabolism, and neurotransmission.
Article
Chemistry, Analytical
Yassel Ramos, Alexis Almeida, Jenis Carpio, Arielis Rodriguez-Ulloa, Yasser Perera, Luis J. Gonzalez, Jacek R. Wisniewski, Vladimir Besada
Summary: In this study, a fractionator device based on continuous gel electrophoresis/electroelution sorting was created for effective separation of complex protein and peptide mixtures. The device showed higher resolution and protein recovery compared to conventional fractionation methods, leading to an improved proteome characterization.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Maria J. Sarmento, Alicia Llorente, Toni Petan, Denis Khnykin, Iuliana Popa, Matea Nikolac Perkovic, Marcela Konjevod, Morana Jaganjac
Summary: Lipids in cell membranes and subcellular compartments are important for cellular processes, and specific organelle lipidomes are characterized by lipid synthesis, metabolism, trafficking, and homeostasis. Identifying the lipid fingerprints of cellular organelles has been challenging due to the variety and complexity of lipids, and the variability in their abundance. This review summarizes the available information on the lipid composition of mammalian cell organelles and discusses the dynamics of subcellular lipidomes under physiological and pathological conditions, as well as the challenges in mapping organelle lipidomes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Vladimir Skalicky, Tereza Vojtkova, Ales Pencik, Jan Vrana, Katarzyna Juzon, Veronika Kolackova, Michaela Sedlarova, Martin F. Kubes, Ondrej Novak
Summary: This study tested various nuclear isolation protocols and combined with mass spectrometry analysis to obtain information on the auxinome from isolated nuclei of Arabidopsis and tobacco cell cultures, revealing dynamic changes in auxin homeostasis within the nucleus. The methodological procedure combining flow cytometry and mass spectrometry offers new horizons for the study of auxin homeostasis at the subcellular level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Forestry
Wiebke Miehe, Laura Czempik, Franz Klebl, Gertrud Lohaus
Summary: Phloem loading mechanisms in herbaceous plants are well studied, but less is known for trees. This study examined tissue-specific sugar and starch contents, as well as sugar concentration in mesophyll cells of different-sized trees, and found that phloem loading in tall trees involves active steps and may involve sucrose uptake transporters (SUTs).
Article
Engineering, Environmental
Yu Sumei, Li Xin, He Shuhong, Zhang Hongchao, Jin Maojun, Zheng Yongquan, Zhang Luqing, Yu Yunlong
Summary: This study investigated the uptake, translocation, and accumulation of triadimefon in wheat plants. Results showed that triadimefon was mainly absorbed by wheat roots through apoplastic pathway and distributed into water soluble fractions. Its accumulation in plant tissues was governed by adsorption onto soils and correlated with concentration in in situ pore water. The study provides valuable information for predicting the uptake of triadimefon by wheat plants and guiding its practical application and safety evaluation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Sam B. Choi, Abigail M. Polter, Peter Nemes
Summary: The integration of patch-clamp electrophysiology with high-resolution mass spectrometry enables the identification and quantification of proteins within neurons. By improving sample processing and detection methods, the study was able to detect approximately 275 different proteins in somal aspirates from cultured neurons.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.
Article
Biochemistry & Molecular Biology
Alice Destailleur, Theo Poucet, Cecile Cabasson, Ana Paula Alonso, Jean-Christophe Cocuron, Romain Larbat, Gilles Vercambre, Sophie Colombie, Pierre Petriacq, Marie Helene Andrieu, Bertrand Beauvoit, Yves Gibon, Martine Dieuaide-Noubhani
Summary: During leaf development, there are significant changes in the composition of the vacuole, with mature leaves likely maintaining osmolarity through inorganic molecules.
Article
Biochemical Research Methods
Chia-Feng Tsai, Rui Zhao, Sarah M. Williams, Ronald J. Moore, Kendall Schultz, William B. Chrisler, Ljiljana Pasa-Tolic, Karin D. Rodland, Richard D. Smith, Tujin Shi, Ying Zhu, Tao Liu
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Chemistry, Analytical
Gabe Nagy, Isaac K. Attah, Christopher R. Conant, Weijing Liu, Sandilya V. B. Garimella, Harsha P. Gunawardena, Jared B. Shaw, Richard D. Smith, Yehia M. Ibrahim
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Piliang Xiang, Ying Zhu, Yu Yang, Zhitao Zhao, Sarah M. Williams, Ronald J. Moore, Ryan T. Kelly, Richard D. Smith, Shaorong Liu
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Adam L. Hollerbach, Ailin Li, Aneesh Prabhakaran, Gabe Nagy, Christopher P. Harrilal, Christopher R. Conant, Randolph Norheim, Colby E. Schimelfenig, Gordon A. Anderson, Sandilya V. B. Garimella, Richard D. Smith, Yehia M. Ibrahim
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Jacob Kagan, Robert L. Moritz, Richard Mazurchuk, Je Hyuk Lee, Peter Vasili Kharchenko, Orit Rozenblatt-Rosen, Eytan Ruppin, Fredrik Edfors, Fiona Ginty, Yury Goltsev, James A. Wells, John LaCava, Jessica L. Riesterer, Ronald N. Germain, Tujin Shi, Mark S. Chee, Bogdan A. Budnik, John R. Yates, Brian T. Chait, Jeffery R. Moffitt, Richard D. Smith, Sudhir Srivastava
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Oncology
Yuqian Gao, Yi-Ting Wang, Yongmei Chen, Hui Wang, Denise Young, Tujin Shi, Yingjie Song, Athena A. Schepmoes, Claire Kuo, Thomas L. Fillmore, Wei-Jun Qian, Richard D. Smith, Sudhir Srivastava, Jacob Kagan, Albert Dobi, Isabell A. Sesterhenn, Inger L. Rosner, Gyorgy Petrovics, Karin D. Rodland, Shiv Srivastava, Jennifer Cullen, Tao Liu
Article
Chemistry, Analytical
Sarah M. Williams, Andrey Liyu, Chia-Feng Tsai, Ronald J. Moore, Daniel J. Orton, William B. Chrisler, Matthew J. Gaffrey, Tao Liu, Richard D. Smith, Ryan T. Kelly, Ljiljana Pasa-Tolic, Ying Zhu
ANALYTICAL CHEMISTRY
(2020)
Article
Cell Biology
Eric S. Orwoll, Jack Wiedrick, Carrie M. Nielson, Jon Jacobs, Erin S. Baker, Paul Piehowski, Vladislav Petyuk, Yuqian Gao, Tujin Shi, Richard D. Smith, Douglas C. Bauer, Steven R. Cummings, Jodi Lapidus
Article
Biochemical Research Methods
Christopher R. Conant, Isaac K. Attah, Sandilya V. B. Garimella, Gabe Nagy, Aivett Bilbao, Richard D. Smith, Yehia M. Ibrahim
Summary: In this study, five different waveforms were characterized in terms of their transmission efficiency, IMS resolution, and resolving power, with triangle and asymmetric ramp profiles found to offer modestly greater resolution and resolving power compared to others. The research also revealed that separations were generally improved with higher TW amplitudes, moderately improved by lower speeds, but experienced decreases in signal intensity under extreme operating conditions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Ailin Li, Gabe Nagy, Christopher R. Conant, Randolph Norheim, Joon Yong Lee, Cameron Giberson, Adam L. Hollerbach, Venkateshkumar Prabhakaran, Isaac K. Attah, Christopher D. Chouinard, Aneesh Prabhakaran, Richard D. Smith, Yehia M. Ibrahim, Sandilya V. B. Garimella
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Ailin Li, Christopher R. Conant, Xueyun Zheng, Kent J. Bloodsworth, Daniel J. Orton, Sandilya V. B. Garimella, Isaac K. Attah, Gabe Nagy, Richard D. Smith, Yehia M. Ibrahim
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Kendall Martin, Tong Zhang, Pengfei Zhang, William B. Chrisler, Fillmore L. Thomas, Fen Liu, Tao Liu, Wei-Jun Qian, Richard D. Smith, Tujin Shi
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Mathematical & Computational Biology
Yuping Zhang, Zhengqing Ouyang, Wei-Jun Qian, Richard D. Smith, Wing Hung Wong, Ronald W. Davis
STATISTICS AND ITS INTERFACE
(2020)
Article
Medicine, Research & Experimental
Rumie Wakasaki, Katsuyuki Matsushita, Kirsti Golgotiu, Sharon Anderson, Mahaba B. Eiwaz, Daniel J. Orton, Sang Jun Han, H. Thomas Lee, Richard D. Smith, Karin D. Rodland, Paul D. Piehowski, Michael P. Hutchens
Article
Chemistry, Multidisciplinary
Gabe Nagy, Dusan Velickovic, Rosalie K. Chu, Alyssa A. Carrell, David J. Weston, Yehia M. Ibrahim, Christopher R. Anderton, Richard D. Smith
CHEMICAL COMMUNICATIONS
(2019)