Article
Oncology
Shashipavan Chillappagari, Julian Schwarz, Vidyasagar Kesireddy, Jessica Knoell, Martina Korfei, Konrad Hoetzenecker, M. Lienhard Schmitz, Christian Behl, Saverio Bellusci, Andreas Guenther, Poornima Mahavadi
Summary: This study found that modulation of autophagy regulating proteins can be used as a therapeutic strategy for idiopathic pulmonary fibrosis (IPF). By improving autophagy and reducing fibroblast proliferation, fine tuning of this pathway may improve antifibrotic properties and enhance the efficacy of current IPF therapy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Peter-James H. Zushin, Yang Zhou, Audrey Li, Euan A. Ashley, Matthew T. Wheeler, Joseph C. Wu
Summary: Dilated cardiomyopathies (DCM) are a leading cause of heart failure in aging populations. The chaperone protein BAG3 plays a significant role in the development of DCM and the rate at which it progresses to heart failure. In this study, two human induced pluripotent stem cell (iPSC) lines were generated from individuals with mutations in exon 3 of the BAG3 gene and their pluripotency and ability to differentiate into three primary germ layers were validated. These cell lines serve as a valuable model for studying BAG3 inactivation and insufficiency in relation to DCM.
STEM CELL RESEARCH
(2023)
Article
Cell Biology
Huibin Song, Dake Chen, Rong Bai, Yue Feng, Shang Wu, Tiansu Wang, Xuanyan Xia, Jialian Li, Yi-Liang Miao, Bo Zuo, Fenge Li
Summary: Knockout of Bag6 exon24 in mice leads to sub-fertility, impaired blood-testis barrier, increased apoptotic testicular cell rate, and abnormal sperm morphology. ER stress occurs in Bag6 exon24-deficient testes, activating sterol regulatory element-binding transcription factor 2 and up-regulating cytochrome P450 family 51 subfamily A member 1 expression, resulting in high serum testosterone levels. Additionally, serine/arginine-rich splicing factor 1 down-regulates BAG6 exon24-skipped transcripts in porcine Sertoli cells by binding to the 35-51 nt region on BAG6 exon24.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Ruiying Wang, Min Wang, Jiahui Zhou, Ziru Dai, Guibo Sun, Xiaobo Sun
Summary: This study demonstrates that Calenduloside E protects against myocardial ischemia/reperfusion injury by inhibiting calcium overload, potentially through the interaction between LTCC and BAG3.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Plant Sciences
Fuhao Huang, Jinlong Pang, Liansong Xu, Wenwen Niu, Yaoshuai Zhang, Shanshan Li, Xian Li
Summary: The study demonstrates that HDI can inhibit the growth and viability of lung adenocarcinoma cells by inducing ferroptosis. In addition, HDI suppresses tumor growth and increases the expression of ferroptosis-related indicators in tumor tissue. The induction of ferroptosis is achieved by regulating VDAC2/3 activity and the interaction between Bcl2 and Bax.
Article
Endocrinology & Metabolism
Haopeng Xu, Yi Xia, Juan Qin, Jie Xu, Chongyan Li, Yan Wang
Summary: The study demonstrated that LIPUS can improve POF induced by VCD by promoting follicular development, increasing sex hormone secretion, inhibiting excessive follicular atresia, and GCs apoptosis, possibly through increasing Bcl-2 protein expression and decreasing Bax expression in the ovaries.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Priyanka Singh, Sanjay Kumar Bhadada, Divya Dahiya, Uma Nahar Saikia, Ashutosh Kumar Arya, Naresh Sachdeva, Jyotdeep Kaur, Arunanshu Behera, Maria Luisa Brandi, Sudhaker Dhanwada Rao
Summary: The study found that the expression of GCM2 in sporadic parathyroid adenomas is suppressed by promoter hypermethylation and higher levels of H3K9me3. Treatment with DAC can restore GCM2 expression.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Cell Biology
Hagen Koerschgen, Marius Baeken, Daniel Schmitt, Heike Nagel, Christian Behl
Summary: BAG3 acts as a key co-chaperone to HSP70 and may also function as a cargo receptor for client proteins, extending its role in selective macroautophagy and protein quality control. It interacts with LC3B, facilitating the autophagic targeting of BAG3 and its cargo to autophagosomes.
Article
Pharmacology & Pharmacy
M. Devgun, S. Lal
Summary: B-cell lymphoma 2-associated athanogene 3 protein is highly expressed in various cancer tissues. Multiple methods were used to analyze gene data and identify genes with similar expression to B-cell lymphoma 2-associated athanogene 3. Molecular analysis and modeling identified compounds with potential to modulate and manage cancers with high expression of B-cell lymphoma 2-associated athanogene 3.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
Seyyedeh Zahra Habibzadeh, Ali Salehzadeh, Zeinab Moradi-Shoeili, Seyed Ataollah Sadat Shandiz
Summary: This study evaluated the effect of iron oxide nanoparticles (Fe3O4@CPTMOS/TP NPs) on gastric cancer cell line and the expression of BAX, BCL2, and p53 genes. The results indicated that Fe3O4@CPTMOS/TP NPs could induce apoptosis in human gastric cancer cells by increasing the expression of BAX/BCL2 ratio and p53 gene.
INORGANIC AND NANO-METAL CHEMISTRY
(2023)
Article
Plant Sciences
Him K. Shrestha, Yosef Fichman, Nancy L. Engle, Timothy J. Tschaplinski, Ron Mittler, Richard A. Dixon, Robert L. Hettich, Jaime Barros, Paul E. Abraham
Summary: This study investigates the molecular changes resulting from C3H/APX-KD associated lignin modification and negative growth phenotype in Brachypodium distachyon using a multi-omic approach. The results show that reduced C3H/APX alters the abundance of enzymes in the phenylpropanoid biosynthetic pathway, disrupts cellular redox homeostasis, and elicits plant defense responses. A deeper understanding of these pleiotropic phenotypes and potential targets identified in this study could improve the economic feasibility of lignocellulosic biofuel production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Kraikrit Utama, Nopawit Khamto, Puttinan Meepowpan, Paitoon Aobchey, Jiraporn Kantapan, Jomkhwan Meerak, Sittiruk Roytrakul, Padchanee Sangthong
Summary: We modified the chemical structure of 2 ',4 '-dihydroxy-6 '-methoxy-3 ',5 '-dimethylchalcone (DMC, 1) by conjugating it with the amino acids L-alanine (compound 3a) or L-valine (compound 3b) in order to enhance its anticancer activity and water solubility. Both compounds 3a and 3b showed antiproliferative activity in human cervical cancer cell lines, with higher inhibitory concentrations compared to DMC. Mechanistic studies revealed that compound 3a induced cell cycle arrest and apoptosis through upregulation of TP53 and CDKN1A, leading to downregulation of CDK2 and BCL2. Compound 3a also showed potential for interaction with HPV16 E6 protein, suggesting its potential as a candidate for anti-cervical cancer drug development.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Chun Zhao, Balaram Ghosh, Tania Chakraborty, Souvik Roy
Summary: Bavachinin, a flavanone compound from the Chinese herb Fructus Psoraleae, has been shown to possess anti-cancer properties by re-establishing damaged colonic crypts in rats and preventing cancer progression.
BIOTECHNIC & HISTOCHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Samar S. Elblehi, Yasser S. El-Sayed, Mohamed Mohamed Soliman, Mustafa Shukry
Summary: Date palm pollen ethanolic extract (DPPE) can partially alleviate doxorubicin (DOX)-induced cardiotoxicity by improving body and heart weight changes, mitigating elevated cardiac injury markers, and amplifying concentrations of cardiac injury markers.
Article
Gastroenterology & Hepatology
Ke Li, Xiang Deng, Guangjing Feng, Yi Chen
Summary: BAG3 plays a crucial role in drug resistance in gastric cancer cells, affecting migration and apoptosis. Suppression of HSF1 can reduce BAG3 expression and decrease sensitivity to BGJ398. Targeting BAG3 may enhance the antitumor efficacy of BGJ398, providing a potential therapeutic strategy for gastric cancer.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Binbin Tao, Hongling Hu, Ji Chen, Lu Chen, Daji Luo, Yonghua Sun, Feng Ge, Zuoyan Zhu, Vance L. Trudeau, Wei Hu
Summary: Recent research has identified a new subunit of the SIN3A-HDAC complex, called Sinhcaf, that plays a crucial role in germ plasm aggregation and primordial germ cell (PGC) specification. Sinhcaf-dependent histone deacetylation is essential for proper PGC development, as mutations in sinhcaf result in defects in germ plasm aggregation and decreased PGC abundance. Sinhcaf achieves this by regulating the acetylation status of the kif26ab promoter, which affects its transcription and ultimately PGC specification.
Article
Biochemical Research Methods
Bing Wang, Min Wang, Shuzhao Jia, Tao Li, Mingkun Yang, Feng Ge
Summary: This study reveals the role of BANCR in cervical cancer, showing that it promotes cell proliferation and inhibits apoptosis in HeLa cells. Through gene knockout and proteomic analysis, BANCR was found to regulate the expression of 569 proteins in HeLa cells. Further investigation suggests that BANCR exerts its effects by inhibiting miR-582-5p expression and subsequently regulating CREB1 expression.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Junhua Huang, Wenjing Dai, Duncheng Xiao, Qian Xiong, Cuifang Liu, Jie Hu, Feng Ge, Xilan Yu, Shanshan Li
Summary: The S. cerevisiae SAGA complex undergoes acetylation-dependent dimerization in response to environmental changes, allowing for cooperative acetylation of nucleosomes, facilitation of gene transcription, and increased cell survival under stress conditions.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biochemical Research Methods
Dan-yun Lai, Jun-biao Xue, Ping He, He-wei Jiang, Yang Li, Ming-liang Ma, Wei Hong, Jun-ping Yu, Hong-ping Wei, Sheng-ce Tao
Summary: This study presents one-year longitudinal neutralization data of BBIBP-CorV on authentic Omicron, Delta, and wild-type strains, and establishes potential surrogate biomarkers for rapid estimation of neutralization titers.
Article
Multidisciplinary Sciences
Qi Yu, Xuanyunjing Gong, Yue Tong, Min Wang, Kai Duan, Xinyu Zhang, Feng Ge, Xilan Yu, Shanshan Li
Summary: In this study, the researchers found that glycolysis regulates histone modifications and gene expression by activating protein kinase A (PKA) pathway. The catalytic subunit of PKA, Tpk2, phosphorylates Jhd2 to affect its localization and function on chromatin, thereby regulating gene expression, lifespan, and autophagy.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Yumeng Zhang, Yaqi Wang, Wei Wei, Min Wang, Shuzhao Jia, Mingkun Yang, Feng Ge
Summary: Protein homeostasis in cyanobacteria is regulated by a proteolytic complex called ClpXP, which consists of ATPase ClpX and peptidase ClpP. This study aimed to investigate the functions and regulatory networks of ClpX in Synechocystis by identifying 172 differentially expressed proteins upon clpX interruption. The results provide a comprehensive ClpX-regulated protein network and new candidates for future functional studies on ClpX in cyanobacteria.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Yu Wang, Mingkun Yang, Feng Ge, Bin Jiang, Rui Hu, Xin Zhou, Yunhuang Yang, Maili Liu
Summary: This study explored the role of lysine succinylation (Ksuc) in regulating aflatoxin (AF) biosynthesis in naturally isolated Aspergillus flavus strains with high/low AF production. The results revealed that Ksuc plays an important role in regulating AF biosynthesis, affecting sclerotia development and AF synthesis in A. flavus strains.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Qihang Peng, Jin Li, Qian Wu, Pei Wang, Zhongcui Kang, Yiting Deng, Yu Xiao, Peng Zheng, Feng Ge, Ying Chen
Summary: Hepatocellular carcinoma (HCC) is a deadly disease with limited diagnostic and therapeutic options. This study explored the expression and potential functions of ZNF385A and ZNF346 in HCC. The results showed that ZNF385A and ZNF346 were overexpressed in HCC and correlated with poor prognosis. They were also associated with immune infiltration and decreased response to immunotherapy. Knockdown of ZNF385A and ZNF346 inhibited HCC cell proliferation and migration. These findings suggest that ZNF385A and ZNF346 could be valuable biomarkers for HCC diagnosis, prognosis, and immunotherapy response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yinsheng Wu, Lixu Tang, Han Huang, Qi Yu, Bicheng Hu, Gang Wang, Feng Ge, Tailang Yin, Shanshan Li, Xilan Yu
Summary: This study reveals that glycolysis-derived serine biosynthesis activates PKM2 to prevent cell senescence and promote healthy aging. The expression of serine biosynthetic enzyme PHGDH is reduced during senescence, leading to a decrease in intracellular serine. PHGDH prevents premature senescence primarily by enhancing the stability and activity of PKM2 and regulating the transcription of senescence-associated genes.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Qian Wu, Jin Li, Pei Wang, Qihang Peng, Zhongcui Kang, Yiting Deng, Jiayi Li, Dehong Yan, Feng Ge, Ying Chen
Summary: As an important member of the kindlin family, FERMT1 is significantly up-regulated in pancreatic carcinoma (PAAD) and correlated with T stage and histologic grade. High FERMT1 expression is closely connected with poor prognosis and can be used to diagnose PAAD. Furthermore, FERMT1 expression is significantly related to N6-methyladenosine (m(6)A) modification and is associated with immune cell infiltration and the modulation of m(6)A and necroptosis. FERMT1 can serve as a diagnostic and prognostic marker of PAAD.
Article
Multidisciplinary Sciences
Xin Li, Qianyun Mei, Qi Yu, Min Wang, Fei He, Duncheng Xiao, Huan Liu, Feng Ge, Xilan Yu, Shanshan Li
Summary: Autophagy is a critical process for maintaining homeostasis, differentiation, and development. This study identifies Ino80 and H2A.Z as targets for the histone deacetylase Rpd3L complex, elucidating their role in regulating autophagy in response to changes in nutrient availability. The study shows that Rpd3L deacetylates Ino80 and H2A.Z, which protects Ino80 from degradation and represses the transcription of autophagy-related genes. The activity of Rpd3L is enhanced by the TORC1 complex, and its inactivation induces autophagy. This research provides insights into the role of chromatin remodelers and histone variants in modulating autophagy.
Article
Virology
Can Zhang, Long-Feng Lu, Zhuo-Cong Li, Ke-Jia Han, Xue-Li Wang, Dan-Dan Chen, Feng Xiong, Xi-Yin Li, Li Zhou, Feng Ge, Shun Li
Summary: Zebrafish MAP2K7 plays a crucial positive role in the host antiviral process, as it stabilizes host IRF7 and degrades SVCV P protein to protect the host during viral infection.
JOURNAL OF VIROLOGY
(2023)
Article
Plant Sciences
Kun Jia, Mingkun Yang, Xin Liu, Qi Zhang, Gaoxiang Cao, Feng Ge, Jindong Zhao
Summary: This study identified a KAT in cyanobacteria, named cGNAT2, and revealed that cGNAT2 regulates the growth and photosynthesis of cyanobacteria through an acetylation-mediated mechanism.
Article
Microbiology
Zhuo Zhu, Mingkun Yang, Guang Yang, Bei Zhang, Xiaohong Cao, Jun Yuan, Feng Ge, Shihua Wang
Summary: This study identified seven members of the PP2C family of protein phosphatases in A. flavus and revealed the crucial roles of Ptc1 and Ptc2 in conidia development, aflatoxin synthesis, seed infection, and autophagic vesicle formation. The study also highlighted the importance of the Asp residue coordinated with Mg2+ in Ptc1 and Ptc2 activity, thermal stability, and biofunction. Additionally, the interaction between Ptc1/Ptc2 and PGK1 was found to be associated with phosphorylation levels and the regulation of aflatoxin synthesis and autophagic vesicle formation.
Article
Biochemistry & Molecular Biology
Qian Wu, Pei Wang, Qihang Peng, Zhongcui Kang, Yiting Deng, Jiayi Li, Ying Chen, Jin Li, Feng Ge
Summary: The study found that ADGRG2 is highly expressed in hepatocellular carcinoma (HCC) and is associated with poor survival. ADGRG2 may be involved in cancer pathways and immune-related pathways. In vitro experiments demonstrated that inhibiting ADGRG2 can suppress the proliferation and migration of liver cancer cells. ADGRG2 is highly correlated with neutrophils and is associated with inflammatory cytokines and immunotherapy efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)