期刊
JOURNAL OF PROTEOME RESEARCH
卷 8, 期 1, 页码 187-198出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr800285v
关键词
iTRAQ; Shotgun proteomics; Nostoc punctiforme ATCC 29133; Nostoc sp PCC 73102; Tandem-MS; Dinitrogen Fixation; Heterocysts
资金
- Swedish Energy Agency
- Knut and Alice Wallenberg foundation
- Nordic Energy Research Program
- EU/Energy [212508]
- BioModular H<INF>2</INF>
- Carl Trygger Foundation
- Magnus Bergvall Foundation
- EPSRC [GR/S84347/01, EP/E036252/1]
- National Science Foundation [DBI-0516378]
- [043340]
- [GR/A11311/01]
- Engineering and Physical Sciences Research Council [EP/E036252/1, GR/S84347/01] Funding Source: researchfish
Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N-2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level and the heterocyst level are presented using isobaric tagging technology (iTRAQ), with 721 proteins at the 95% confidence interval quantified across both studies. Observations from both experiments yielded findings confirmatory of both transcriptional studies, and published Nostoc sp. PCC 7120 iTRAQ data. N. punctiforme exhibits similar metabolic trends, though changes in a number of metabolic pathways are less pronounced than in Nostoc sp. PCC 7120. Results also suggest a number of proteins that may benefit from future investigations. These include ATP dependent Zn-proteases, N-reserve degraders and also redox balance proteins. Complementary proteomic data sets from both organisms present key precursor knowledge that is important for future cyanobacterial biohydrogen research.
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