期刊
JOURNAL OF PROTEOME RESEARCH
卷 8, 期 4, 页码 1859-1869出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr801094b
关键词
healthy colorectal; cancer colorectal; ex vivo High-Resolution Magic-Angle Spinning NMR spectroscopy; biochemical markers; Principal Component Analysis; Partial Least Squares Discriminant Analysis
资金
- The Fondazione Cassa di Risparmio di Modena
- MIUR
The metabolic profile of human healthy and neoplastic colorectal tissues was obtained using ex vivo High-Resolution Magic Angle Spinning (HR-MAS) NMR spectroscopy. Principal Components Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) were applied to NMR data in order to highlight the biochemical differences between healthy and neoplastic colorectal tissues. The synergic combination of ex vivo HR-MAS NMR spectroscopy with Multivariate Data Analysis enables discrimination between healthy and tumoral colorectal tissues and identification of the increase of taurine, acetate, lactate, and lipids, and the decrease of polyols and sugars as tumoral characteristics. Moreover, it was found that macroscopically/histologically normal colorectal tissues, collected at least 15 cm from the adenocarcinoma, are characterized by a metabolic pattern quite similar to that typical of tumoral lesions. It was shown that ex vivo HR-MAS NMR spectroscopy, performed on intact specimens, may be of great potentiality in the clinical evaluation of human neoplastic colorectal tissues and that the biochemical data represent the molecular basis for an accurate and noninvasive clinical applications of in vivo NMR spectroscopy.
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