期刊
JOURNAL OF PROTEOME RESEARCH
卷 8, 期 11, 页码 5382-5386出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr9003932
关键词
spermatozoa infertility; sperm motility; protein phosphorylation; gamma-tubulin
资金
- Public Health Bureau in Taipei, Taiwan [96001-62-045, TSGH-C98-64]
The majority of male infertility results from poor sperm motility A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.
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