Article
Multidisciplinary Sciences
Agnieszka Gegotek, Sinemyiz Atalay, Elzbieta Skrzydlewska
Summary: The study found significant changes in protein expression in rat plasma after UV irradiation and CBD treatment, including anti-inflammatory and signaling proteins. CBD prevented these changes to some extent and directly modified the activity of specific proteins by creating adducts with them.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Egil Ferkingstad, Patrick Sulem, Bjarni A. Atlason, Gardar Sveinbjornsson, Magnus I. Magnusson, Edda L. Styrmisdottir, Kristbjorg Gunnarsdottir, Agnar Helgason, Asmundur Oddsson, Bjarni V. Halldorsson, Brynjar O. Jensson, Florian Zink, Gisli H. Halldorsson, Gisli Masson, Gudny A. Arnadottir, Hildigunnur Katrinardottir, Kristinn Juliusson, Magnus K. Magnusson, Olafur Th. Magnusson, Run Fridriksdottir, Saedis Saevarsdottir, Sigurjon A. Gudjonsson, Simon N. Stacey, Solvi Rognvaldsson, Thjodbjorg Eiriksdottir, Thorunn A. Olafsdottir, Valgerdur Steinthorsdottir, Vinicius Tragante, Magnus O. Ulfarsson, Hreinn Stefansson, Ingileif Jonsdottir, Hilma Holm, Thorunn Rafnar, Pall Melsted, Jona Saemundsdottir, Gudmundur L. Norddahl, Sigrun H. Lund, Daniel F. Gudbjartsson, Unnur Thorsteinsdottir, Kari Stefansson
Summary: Genome-wide association studies of plasma protein levels in Icelanders have identified numerous associations with diseases and other traits, providing valuable insights into disease pathogenesis and potential drug targets. Through integration of proteomics, genomics, and transcriptomics, this research offers a resource for improving disease understanding and aiding drug discovery and development.
Article
Chemistry, Multidisciplinary
Amelie I. S. Sobczak, Kondwani G. H. Katundu, Fladia A. Phoenix, Siavash Khazaipoul, Ruitao Yu, Fanuel Lampiao, Fiona Stefanowicz, Claudia A. Blindauer, Samantha J. Pitt, Terry K. Smith, Ramzi A. Ajjan, Alan J. Stewart
Summary: This study reveals that under certain conditions, specific non-esterified fatty acids (NEFAs) can interfere with human serum albumin (HSA) buffering capacity for zinc (Zn2+), impacting the cellular and protein aspects of coagulation.
Review
Biochemical Research Methods
Eric W. Deutsch, Gilbert S. Omenn, Zhi Sun, Michal Maes, Maria Pernemalm, Krishnan K. Palaniappan, Natasha Letunica, Yves Vandenbrouck, Virginie Brun, Sheng-ce Tao, Xiaobo Yu, Philipp E. Geyer, Vera Ignjatovic, Robert L. Moritz, Jochen M. Schwenk
Summary: The study of proteins circulating in blood presents significant opportunities for diagnosing, stratifying, or preventing diseases. Recent technological advancements have made the proteomic analysis of blood-derived serum and plasma increasingly important in understanding human biology and pathophysiology. By utilizing mass-spectrometry (MS) or affinity-based methods, valuable insights into SARS-CoV-2 infections, aging, and hemostasis have been gained, along with the potential to combine proteomics with genetic data for further insights. The ongoing challenges include translating plasma proteomics into precision medicine, with recent advances in the field providing impactful applications.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Avgustina Danailova, Svetla Todinova, Lidia Gartcheva, Desislava Bogdanova, Elena Zlatareva, Nikolay Kalaydzhiev, Ivan Milanov, Sashka Krumova, Stefka G. Taneva
Summary: Discovery of diagnostic biomarkers for age-related neurodegenerative pathologies (NDDs) is crucial for accurate diagnosis, disease progression monitoring, and drug development. This study used differential scanning calorimetry to evaluate thermodynamic changes in the blood plasma proteome, aiming to identify new biomarkers for Parkinson's disease (PD) and amyotrophic lateral sclerosis (ALS). The thermodynamic behavior of plasma proteins reflected the complexity and heterogeneity of the two diseases, and the unfolding temperature of the protein albumin and the calorimetric profile center were identified as thermodynamic signatures. Stratification of PD and ALS patients based on these signatures was successful, except for a few cases with significantly different thermodynamic parameters. Additionally, capillary electrophoresis revealed altered globulin levels in PD and ALS patients' plasma. These findings highlight the potential of calorimetry and electrophoresis in indirectly identifying NDDs' biomarkers in blood plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Mohamed A. Elhadad, Rory Wilson, Shaza B. Zaghlool, Cornelia Huth, Christian Gieger, Harald Grallert, Johannes Graumann, Wolfgang Rathmann, Wolfgang Koenig, Moritz F. Sinner, Kristian Hveem, Karsten Suhre, Barbara Thorand, Christian Jonasson, Melanie Waldenberger, Annette Peters
Summary: The study identified associations between MetS and 116 plasma proteins, with 53 replicating in other studies. Incident MetS was associated with 14 proteins, 13 of which overlapped with prevalent MetS associated proteins, and one was unique to incident MetS. Mendelian randomization suggested causal effects of three proteins on MetS: APOE2, APOB, and RET.
CARDIOVASCULAR DIABETOLOGY
(2021)
Article
Chemistry, Analytical
Farshad Ardakani, Bahram Hemmateenejad
Summary: In this study, a new type of microfluidic paper-based analytical device (μPAD) was developed for the separation of plasma from whole blood without using plasma separation membrane. By controlling the pore size of the paper through lamination and electrolyte addition, the device achieved a longer separation distance for plasma with no significant hemolysis. The device has the potential for multi-assay testing in point-of-care experiments.
ANALYTICA CHIMICA ACTA
(2023)
Article
Multidisciplinary Sciences
Shashi Gupta, Matthew J. Westacott, Deborah G. Ayers, Sophie J. Weiss, Penn Whitley, Christopher Mueller, Daniel C. Weaver, Daniel J. Schneider, Anis Karimpour-Fard, Lawrence E. Hunter, Daniel W. Drolet, Nebojsa Janjic
Summary: Early detection of cancer is crucial, but currently many cancers are diagnosed at an advanced stage. Researchers have studied the changes in the plasma proteome of mice with transplanted human tumors to gain insight into the value of protein biomarkers for early detection and stratification of cancer. They used an aptamer-based assay to measure around 5000 proteins and identified common markers as well as pathways among different types of cancer.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Adi L. Tarca, Roberto Romero, Gaurav Bhatti, Francesca Gotsch, Bogdan Done, Dereje W. Gudicha, Dahiana M. Gallo, Eunjung Jung, Roger Pique-Regi, Stanley M. Berry, Tinnakorn Chaiworapongsa, Nardhy Gomez-Lopez
Summary: The human plasma proteome was analyzed using an aptamer-based platform, revealing associations between gestational age and changes in proteins related to growth regulation, angiogenesis, immunity, and inflammation. Machine learning was able to accurately predict gestational age and time to term delivery, indicating the potential of the plasma proteome as a non-invasive tool for investigating obstetrical diseases.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Hematology
Howard Lin, Brooks P. Scull, Baruch R. Goldberg, Harshal A. Abhyankar, Olive E. Eckstein, Daniel J. Zinn, Joseph Lubega, Jennifer Agrusa, Nader El Mallawaney, Nitya Gulati, Lisa Forbes, Ivan Chinn, Rikhia Chakraborty, Jessica Velasquez, Jordana Goldman, Dalia Bashir, Fong Lam, Eyal Muscal, Michael M. Henry, Jay N. Greenberg, Stephan Ladisch, Michelle L. Hermiston, Lauren K. Meyer, Michael Jeng, Ahmed Naqvi, Kenneth McClain, Trung Nguyen, Hector Wong, Tsz-Kwong Man, Michael B. Jordan, Carl E. Allen
Summary: HLH is characterized by pathologic immune activation, and prompt initiation of immune suppression is crucial for survival. Plasma biomarkers can differentiate HLH from other inflammatory conditions, with IFN-gamma signaling uniquely elevated in HLH.
Article
Immunology
Stephen A. Rawlings, Felix Torres, Alan Wells, Andrea Lisco, Wendy Fitzgerald, Leonid Margolis, Sara Gianella, Christophe Vanpouille
Summary: HIV infection disrupts the cytokine network, and this disruption is not completely reversed by ART. This study characterized the cytokine network in the blood and genital secretions of HIV-infected individuals before and after ART. The results showed that ART impacts the cytokine network in semen, while the overall effect on the network in blood is modest.
Article
Multidisciplinary Sciences
Lingyan Chen, James E. Peters, Bram Prins, Elodie Persyn, Matthew Traylor, Praveen Surendran, Savita Karthikeyan, Ekaterina Yonova-Doing, Emanuele Di Angelantonio, David J. Roberts, Nicholas A. Watkins, Willem H. Ouwehand, John Danesh, Cathryn M. Lewis, Paola G. Bronson, Hugh S. Markus, Stephen Burgess, Adam S. Butterworth, Joanna M. M. Howson
Summary: In this study, the researchers used a Mendelian randomization framework to estimate the causal effects of 308 plasma proteins on stroke outcomes. They identified genetic associations between several plasma proteins and stroke, as well as identified potential therapeutic targets and risk factors for stroke.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Z. Jaksic, V Mrljak, A. Horvatic, A. Gelemanovic, M. Micic
Summary: The study aimed to monitor and analyze the plasma proteome profiles and biochemistry parameters of injured and diseased loggerhead sea turtles during their recovery. Valuable biomarkers of turtle's health were identified, along with proteins modulated in different age groups and recovery periods. Certain plasma proteins were highlighted as potential biomarkers for turtle's health status.
JOURNAL OF PROTEOMICS
(2022)
Article
Chemistry, Multidisciplinary
Yuhao Ma, Narges Hadjesfandiari, Michael Doschak, Dana Devine, Marcello Tonelli, Larry Unsworth
Summary: This study focused on the removal of carbamylated plasma proteins through adsorption onto a surface functionalized with a specific peptide. NH-cH2p1 showed superior selectivity compared to CO-cH2p1, maintained adsorption capacity in diluted plasma, and exhibited low platelet adhesion and activation, suggesting that the designed surface does not adversely affect platelets.
Article
Cell Biology
Lijie Zhou, Yingwei Ge, Yesheng Fu, Bo Wu, Yong Zhang, Lei Li, Chun-Ping Cui, Siying Wang, Lingqiang Zhang
Summary: By systematically screening up to 20,000 proteins using a human proteome microarray, potential interacting proteins of LUBAC and OTULIN were identified, suggesting they may function as regulators or substrates of linear ubiquitination. Additionally, the results hint at the broad functions of linear ubiquitination in diverse pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Joanna L. Richens, Jordan S. Lane, Jonathan P. Bramble, Paul O'Shea
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2015)
Article
Biochemistry & Molecular Biology
George R. Heath, Mengqiu Li, Isabelle L. Polignano, Joanna L. Richens, Gianluca Catucci, Paul O'Shea, Sheila J. Sadeghi, Gianfranco Gilardi, Julea N. Butt, Lars J. C. Jeuken
Article
Chemistry, Multidisciplinary
A. J. Wright, J. L. Richens, J. P. Bramble, N. Cathcart, V. Kitaev, P. O'Shea, A. J. Hudson
Article
Multidisciplinary Sciences
Qimei Zhang, Stephen P. Morgan, Paul O'Shea, Melissa L. Mather
Article
Chemistry, Physical
Miguel A. Gonzalez, Hanna M. G. Barriga, Joanna L. Richens, Robert V. Law, Paul O'Shea, Fernando Bresme
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2017)
Article
Chemistry, Organic
Kimberley J. Powell, Joanna L. Richens, Jonathan P. Bramble, Li-Chen Han, Pallavi Sharma, Paul O'Shea, John E. Moses
Article
Multidisciplinary Sciences
Joanna L. Richens, Hannah L. Spencer, Molly Butler, Fiona Cantlay, Kelly-Ann Vere, Nin Bajaj, Kevin Morgan, Paul O'Shea
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Joanna L. Richens, Arwen I. I. Tyler, Hanna M. G. Barriga, Jonathan P. Bramble, Robert V. Law, Nicholas J. Brooks, John M. Seddon, Oscar Ces, Paul O'Shea
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Claire Teevan-Hanman, Paul O'Shea
Summary: This study investigates serum albumin binding to the yeast form of Candida albicans and identifies two populations of binding sites with different affinities and relative abundances, potentially affecting total binding capacities. The low-affinity binding sites are associated with yeast cell aggregation. This research also explores the dependence of these values on C. albicans strain and culture media, speculating on physiological consequences related to immune evasion, biofilm formation, and interkingdom signaling processes.
Article
Hematology
Chris Moore, Joanna L. Richens, Yasmin Hough, Deniz Ucanok, Sunir Malla, Fei Sang, Yan Chen, Stone Elworthy, Robert N. Wilkinson, Martin Gering