Article
Chemistry, Multidisciplinary
Wenjing Ma, Zhuojia Xu, Yuhan Jiang, Jialin Liu, Dandan Xu, Wei Huang, Tiehai Li
Summary: This study synthesized a library of IgG N-glycans covering all possible biantennary and bisected structures by reprogramming biosynthetic assembly lines and deciphered their unique binding specificities with different proteins using glycan microarray technology. This synthetic IgG N-glycan library can be used as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics for biomedical applications.
Article
Chemistry, Multidisciplinary
Jose Danglad-Flores, Sabrina Leichnitz, Eric T. Sletten, A. Abragam Joseph, Klaus Bienert, Kim Le Mai Hoang, Peter H. Seeberger
Summary: The new AGA synthesizer offers better temperature control, more protecting group options, and shorter coupling cycles, enabling rapid and efficient synthesis of complex carbohydrates and expanding the range of glycans that can be prepared.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Eric J. Carpenter, Shaurya Seth, Noel Yue, Russell Greiner, Ratmir Derda
Summary: The GlyNet model accurately predicts glycan-protein interactions, providing an important benchmark for quantitative computational glycobiology.
Article
Chemistry, Organic
Zhiyuan Chen, Guozhi Xiao
Summary: The highly branched tetradecasaccharide repeating unit and shorter sequences of GLSWA-1 from Ganoderma lucidum, which possess immune-enhancing activities, were prepared using a one-pot glycan assembly strategy that features orthogonal one-pot glycosylation, one-pot assembly of oligosaccharides, and modular and convergent [4+5+5] assembly of the highly branched tetradecasaccharide.
Review
Spectroscopy
David J. Harvey
Summary: This review provides an update on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates. It covers the literature up to 2020 and includes methods appropriate to MALDI analysis. The review is divided into three sections, focusing on general aspects, applications to various structural types, and other areas where MALDI is extensively used. The reported work demonstrates the increasing use of new techniques and the significant impact of MALDI imaging.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Immunology
Laudine M. C. Petralia, Angela van Diepen, Dieu-Linh Nguyen, Lena A. Lokker, Erliyani Sartono, Sasisekhar Bennuru, Thomas B. Nutman, Kenneth Pfarr, Achim Hoerauf, Samuel Wanji, Jeremy M. Foster, Cornelis H. Hokke
Summary: Parasitic nematodes cause chronic disablement in humans worldwide. Current diagnostics for surveillance of filarial diseases have limitations, and improved assays are needed. We studied the glycan antigens of the parasite Brugia malayi and found that they triggered IgG responses. Our findings suggest potential for developing disease-specific diagnostics for filarial disease control.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Gradimir Misevic, Emanuela Garbarino
Summary: The binding of glycans to glycans plays a significant role in xenogeneic self-recognition and adhesion in sponges, cell compaction in mouse embryos, and in vitro tumor cell adhesion in mammals. This intermolecular recognition process is considered a new paradigm in cellular interactions. Glycan structures in sponges have unique species-specific sequences, and their binding mechanism relies on specific interactions of small oligosaccharide motifs.
Review
Biochemistry & Molecular Biology
Leonid M. Kustov, Alexander L. Kustov, Tapio Salmi
Summary: In recent years, the catalytic conversion of carbohydrates into value-added products and platform chemicals has become a trend. The use of microwave activation and proper catalyst selection can significantly enhance the efficiency and selectivity of catalysts. This mini-review provides a brief literature survey on microwave technologies used for processing carbohydrates, with a focus on the use of heterogeneous catalysts and hybrid materials.
Article
Chemistry, Multidisciplinary
Tan Phat Vo, Nu To Uyen Nguyen, Viet Ha Le, Thuy Han Phan, Thi Hoang Yen Nguyen, Dinh Quan Nguyen
Summary: This study optimized the extraction processes of phenolics and flavonoids from passion fruit peels using ultrasound and microwave methods. The optimal conditions for both methods were determined and the extraction mechanisms and thermodynamic parameters were investigated. The extracts obtained under the optimized conditions showed strong antioxidant activities.
Article
Food Science & Technology
Wenqi Cai, Hongyun Zhang, XinRu Chen, Shiyu Yan, Lina Yang, Hong Song, Jun Li, Jun Liu, Hansong Yu, He Liu, Danshi Zhu
Summary: This study investigated the impact of extraction method on the physicochemical and structural properties of soy hull polysaccharide (SHP). The results indicated that the polysaccharide extraction rate and composition varied with different extraction methods, but the main chain composition and molecular weight were similar.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Materials Science, Paper & Wood
Gabriel Abranches Dias Castro, Neide Paloma Goncalves Lopes, Sergio Antonio Fernandes, Marcio Jose da Silva
Summary: This paper presents a novel approach to synthesize 5-hydroxymethylfurfural (HMF) using metal-exchanged Keggin heteropolyacid salts as catalysts, resulting in improved yield and reduced environmental impact. The most efficient catalyst, Cu3/2PW12O40, showed high Lewis acidic strength allowing for coordination with water molecules and generation of H3O+ ions in the reaction medium.
Article
Acoustics
Yanlin Zhang, Yi Lei, Shirong Qi, Mingxuan Fan, Shuyi Zheng, Qingbin Huang, Xu Lu
Summary: The purpose of this study was to investigate the effects of different extraction methods on the yield, chemical structures and antioxidant activity of Dictyo-phora indusiata polysaccharides. The results showed that ultrasonic-microwave-assisted extraction (UMAE) had the greatest damage to the cell wall of the polysaccharides and the best comprehensive antioxidant capacity. Different extraction methods had no significant effect on the types of glycosidic bonds and sugar rings, but there were differences in absolute molecular weight and molecular conformation. UMAE had the highest polysaccharides yield, indicating its potential for modification and application in the functional food industry.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Mariana Spinei, Mircea Oroian
Summary: This study analyzed the factors and optimal conditions for extracting pectin from grape pomace using microwave technique, and compared the extracted pectin with commercial pectin. The results showed that grape pomace has the potential to be a valuable source of pectin.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Martin Fanzone, Ignacio Coronado, Santiago Sari, Anibal Catania, Mariona Gil i Cortiella, Mariela Assof, Viviana Jofre, Cristina Ubeda, Alvaro Pena-Neira
Summary: This study evaluated the combined effect of microwave-assisted extraction and stem additions on the chemical composition of Bonarda wines. The results showed that stem additions increased tannin content, while microwave-assisted extraction improved phenolic extraction and polymeric pigments formation, enhancing wine color. In addition, the combined strategies increased polysaccharides extraction and modified the volatile profile of wines.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Xue-Qing Liu, Xiao-Hui Yan, Jun Liang, Hai-Xue Kuang, Yong -Gang Xia
Summary: A sample preparation method based on microwave assisted free radical degradation (MFRD) was proposed to establish structural-fingerprinting of polysaccharides for quality assessment. The MFRD condition was optimized and the structures of the degradation products were analyzed. A low-polymerization compositional fingerprinting was successfully constructed using high-performance anion-exchange chromatography. Compared to conventional methods, MFRD significantly improves sample preparations and is applicable to various polysaccharide samples.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
