期刊
JOURNAL OF PROTEOME RESEARCH
卷 7, 期 9, 页码 4095-4106出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr700687c
关键词
de novo sequencing; underrepresented species in databases; multigenic families; polyphenol oxidase; phylogenetic analysis
资金
- Spanish Ministry of Education, Science and Sports [AGF99-0396, BIO2002-03100]
- FEDER
Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed.
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