Article
Biochemistry & Molecular Biology
Riichi Oguchi, Kousuke Hanada, Minami Shimizu, Masako Mishio, Hiroshi Ozaki, Kouki Hikosaka
Summary: By studying the growth data and whole genome of Arabidopsis thaliana ecotypes, we identified two genes associated with enhancement of growth rate under elevated CO2 conditions. These findings are important for understanding plant growth and agricultural yield under future global climate change.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Toshiyuki Sakai, Akira Abe, Motoki Shimizu, Ryohei Terauchi
Summary: Characterizing epistatic gene interactions is crucial for understanding complex traits, and using recombinant inbred lines can provide a simple and interpretable method for detecting such interactions.
G3-GENES GENOMES GENETICS
(2021)
Article
Agronomy
Vikrant Khare, Rama Shankar Shukla, Sanjay Kumar Singh, Suneeta Pandey
Summary: This study explores the variation and recognition of heat responsive traits in wheat, aiming to enhance wheat productivity in high temperature stress conditions. The findings provide valuable insights into the key factors influencing grain yield and offer a framework for developing heat tolerant cultivars in the future.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2024)
Article
Physics, Multidisciplinary
Shahaf Armon, Michael Moshe, Eran Sharon
Summary: Plant leaves grow in a decentralized manner, with cell deformation maintaining shape while experiencing fluctuations, as shown by high-resolution measurement of Tobacco leaf growth. These fluctuations must be regulated and correlated for flat leaf growth.
COMMUNICATIONS PHYSICS
(2021)
Article
Agronomy
Ana Lopez-Malvar, Zoila Resendiz, Rogelio Santiago, Jose Cruz Jimenez-Galindo, Rosa Ana Malvar
Summary: Corn borers are the most important pest affecting maize, and resistance to them can compromise plant fitness and yield. Stronger resistant maize lines may exhibit higher concentrations of cell wall-bound hydroxycinnamates, but also characteristics like early maturity, low grain moisture, and reduced plant height. Selecting for resistance may therefore have negative impacts on yield. A breeding strategy focusing on grain yield to tolerate corn borer attack is recommended.
Article
Plant Sciences
Xinyi Zhou, Wei Xie, Hao Jing, Junru Fu, Maomao Li, Jianmin Bian, Jie Xu, Yicong Cai, Haohua He, Dahu Zhou
Summary: This study used a functional rice variety, Zibaoxiangnuo 1 (ZBXN 1), rich in total flavonoids and anthocyanins, as the experimental material, and crossed it with Minghui63 (MH63), a variety without anthocyanins, to construct Recombination Inbred Lines (RILs). The contents of anthocyanins and total flavonoids in RILs and two parents were determined for three consecutive generations. The results showed that ZBXN 1 had abundant and stable anthocyanins, making it a potential breeding material for high-quality rice varieties with high anthocyanin content.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
A. E. Stanley, A. Menkir, B. Ifie, A. A. Paterne, N. N. Unachukwu, S. Meseka, W. A. Mengesha, B. Bossey, O. Kwadwo, P. B. Tongoona, O. Oladejo, C. Sneller, M. Gedil
Summary: This study utilized a genome-wide association study to identify key SNPs associated with Striga resistance and proposed candidate genes for improving maize Striga resistance breeding. High heritability estimates were observed under Striga infested conditions, and various multi-locus approaches were employed to identify relevant SNPs. Marker-effect prediction helped in identifying alleles responsible for high yield and low Striga damage rating in the breeding panel.
SCIENTIFIC REPORTS
(2021)
Article
Agronomy
Attiq Ur Rehman, Ghulam Shabbir, Zahid Akram, Abid Riaz
Summary: This study developed recombinant inbred lines (RILs) to explore genetic resistance to Karnal bunt (KB) in wheat. Several RILs with durable and heritable resistance were identified, and specific genomic regions associated with resistance were identified through SNP genotyping. These findings provide valuable breeding materials and insights for developing KB-resistant wheat cultivars in the future.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Plant Sciences
Eva van Zelm, Silvia Bugallo-Alfageme, Pariya Behrouzi, A. Jessica Meyer, Christa Testerink, Charlotte M. M. Gommers
Summary: The root system architecture of plants changes during salt stress exposure. Different accessions of Arabidopsis thaliana adopt different strategies in remodeling their root architecture during salt stress. Salt induces a multiphase growth response in roots, and both the growth rate of main roots during homoeostasis and lateral root appearance are the strongest determinants of overall root architecture. Furthermore, a trade-off between investing in main or lateral root length during salt stress is revealed. By studying natural variation in high-resolution temporal root growth using mathematical modeling, new insights in the interactions between dynamic root growth traits are discovered.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Agronomy
Subhash Chandra, Akshay Talukdar, Yashpal Taak, Raju R. Yadav, Manisha Saini, N. S. Sipani
Summary: The study showed that different soybean genotypes exhibited high germination rates when fresh, but the germination rates decreased significantly with storage time. Among the genotypes tested, Glycine tomentella showed the highest seed storability, while Glycine max exhibited lower viability.
GENETIC RESOURCES AND CROP EVOLUTION
(2022)
Article
Plant Sciences
Nicholas de Jager, Varsa Shukla, Anna Koprivova, Martin Lycka, Lorina Bilalli, Yanrong You, Juergen Zeier, Stanislav Kopriva, Daniela Ristova
Summary: Sulfur is an essential mineral nutrient for plant growth and development. Its content in leaves is tightly connected with sulfate uptake, concentration of sulfate and phosphate anions, and synthesis of glucosinolates and glutathione. Sulfur homeostasis in Arabidopsis thaliana is regulated by biotic and abiotic stressors, leading to large variation in sulfur content among different accessions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemical Research Methods
Narges Sahranavard, Eisa Jorjani, Hossein Sabouri, Sharifeh Mohahamad Alegh, Mahnaz Katouzi
Summary: The aim of this study was to identify the genomic regions controlling traits related to grains and leaves in wheat. Through genotyping and phenotyping, 10 quantitative trait loci were identified for grain and leaf traits. These findings help to understand the genetic mechanisms of wheat and support marker-assisted breeding programs.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Multidisciplinary Sciences
Martin Schafer, Alan R. Pacheco, Rahel Kunzler, Miriam Bortfeld-Miller, Christopher M. Field, Evangelia Vayena, Vassily Hatzimanikatis, Julia A. Vorholt
Summary: Resource allocation affects the structure of microbiomes, including those associated with living hosts. Understanding the degree to which this dependency determines interspecies interactions may advance efforts to control host-microbiome relationships. We combined synthetic community experiments with computational models to predict interaction outcomes between plant-associated bacteria. The models recapitulated outcomes observed in planta with >89% accuracy, highlighting the role of carbon utilization and the contributions of niche partitioning and cross-feeding in the assembly of leaf microbiomes.
Article
Plant Sciences
Tripti Singhal, C. Tara Satyavathi, S. P. Singh, Aruna Kumar, S. Mukesh Sankar, C. Bhardwaj, M. Mallik, Jayant Bhat, N. Anuradha, Nirupma Singh
Summary: This study identified QTLs for iron and zinc content in pearl millet, which could be valuable for improving the quality and yield of this crop through genetic manipulation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Asmaa Allaoui, Eric N. Jellen, El Hadji Thiam, Ouafae Benlhabib
Summary: There is a growing interest in expanding quinoa cultivation due to its sensitivity to high temperatures. In this study, the potential of two quinoa recombinant inbred lines (RILs), RIL2-4 and RIL2-5, was assessed for seed setting rate and production potential under high temperatures.
CHILEAN JOURNAL OF AGRICULTURAL RESEARCH
(2023)
Article
Plant Sciences
Aurora Alaguero-Cordovilla, Ana Belen Sanchez-Garcia, Sergio Ibanez, Alfonso Albacete, Antonio Cano, Manuel Acosta, Jose Manuel Perez-Perez
Summary: The study characterized the formation of adventitious roots in tomato seedlings after whole root excision using physiological, genetic, and molecular approaches. It was found that regulated auxin transport and biosynthesis are crucial for re-establishing internal auxin gradients, leading to cell proliferation and the formation of new adventitious roots at specific positions.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Agronomy
Virginia Birlanga, Jose Ramon Acosta-Motos, Jose Manuel Perez-Perez
Summary: Study of 12 lettuce genotypes showed genotype x environment interactions in different growing seasons, with nutrient traits highly correlated with tipburn incidence. The experimental setup allows selection of tipburn-tolerant and highly productive lettuce genotypes suitable for hydroponics.
Article
Plant Sciences
Taras Pasternak, Stefan Kircher, Jose Manuel Perez Perez, Klaus Palme
Summary: A simple pipeline of marker-free histological techniques enables the precise mapping of cell cycle stages in the root apical meristem, allowing for dynamic chromatin studies. This study presents a pipeline that simultaneously determines cellular parameters, nucleus geometry, and cell cycle kinetics in the root apical meristem (RAM). The method uses marker-free techniques for nucleus and cell boundary detection and EdU staining for DNA replication quantification. The study finds that differences in cell volume, nucleus volume, and nucleus shape exist across different domains of the Arabidopsis RAM, and DNA replication patterns are influenced by cell layer and region. The duration of the G2 phase and endocycle are also associated with certain features of nucleus geometry.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Virginia Birlanga, Jose Ramon Acosta-Motos, Jose Manuel Perez-Perez
Summary: Extreme weather events caused by human-driven climate change can potentially impact global agricultural production. Soilless production systems are a solution to optimize natural resource use, but environmental factors may cause nutritional imbalances and affect crop production.
Article
Plant Sciences
Maria Salud Justamante, Mariem Mhimdi, Marta Molina-Perez, Alfonso Albacete, Maria Angeles Moreno, Ines Mataix, Jose Manuel Perez-Perez
Summary: Prunus species are important stone fruits in the Mediterranean region, and rootstocks with specific adaptations are needed. Adventitious root formation is a crucial mechanism for stress tolerance in Prunus sp. Through the evaluation of hardwood cuttings from different rootstocks, genotype-dependent responses in rooting were observed. A time-series study revealed dynamic changes in endogenous hormone homeostasis, which may explain the differences in rooting performance.
Review
Plant Sciences
Sergio Navarro-Cartagena, Jose Luis Micol
Summary: The interaction between auxin and cytokinins plays a role in various aspects of plant development, such as ovule formation and lateral root initiation. Cytokinins also contribute to the complexity of compound leaves in Solanum lycopersicum and Cardamine hirsuta. However, their role in the patterning of simple leaf margins in Arabidopsis thaliana, which is assumed to be explained by auxin localization, has not been proposed before. In this study, we discuss evidence supporting the hypothesis that cytokinins are involved in simple leaf margin morphogenesis through crosstalk with auxin, similar to other plant developmental events. Mutant or transgenic Arabidopsis plants with defects in cytokinin biosynthesis or signaling, or enhanced cytokinin degradation, show less serrated leaf margins compared to the wild type.
TRENDS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Carla Navarro-Quiles, Eduardo Mateo-Bonmati, Hector Candela, Pedro Robles, Antonio Martinez-Laborda, Yolanda Fernandez, Jan Simura, Karin Ljung, Vicente Rubio, Maria Rosa Ponce, Jose Luis Micol
Summary: ABCE proteins play a conserved role in translation in plants, dissociating cytoplasmic ribosomes and contributing to ribosome recycling. In Arabidopsis, the ABCE2 protein is important for general growth and vascular development, likely through indirect effects on auxin metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Larriba, Miriam Nicolas-Albujer, Ana Belen Sanchez-Garcia, Jose Manuel Perez-Perez
Summary: In this study, transcriptomic analysis was conducted to identify gene regulatory networks involved in wound-induced organ regeneration in tomato. The results showed that cell reprogramming and gene activation require spatial and temporal regulation of auxin and cytokinin gradients in the hypocotyl, as well as extensive metabolic reprogramming in the apical region.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Abelardo Modrego, Taras Pasternak, Moutasem Omary, Alfonso Albacete, Antonio Cano, Jose Manuel Perez-Perez, Idan Efroni
Summary: The removal of the root system triggers the formation of new roots from the remaining shoot, a process primarily regulated by the phytohormone auxin. In this study, the tomato mutation rosette (ro) was found to have a deficiency in shoot-borne root formation and reduced auxin transport rates. The ro gene was identified as the tomato ortholog of BIG and UBR4/p600 genes, which are known to regulate auxin transport in Arabidopsis thaliana and calcium homeostasis in mammals. The study suggests that the RO/BIG protein maintains auxin flow by stabilizing the membrane localization of the auxin transporter PIN1, potentially through attenuating the inhibitory effect of calcium on cytoplasmic streaming.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Taras Pasternak, Klaus Palme, Jose Manuel Perez-Perez
Summary: Reactive oxygen species (ROS) play a dual role in plant biology, acting as important signal transduction molecules and as toxic byproducts of aerobic metabolism that accumulate in cells upon exposure to different stressors and lead to cell death. In this study, it was found that ROS is an important modulator of auxin distribution and response in plant roots. Genetic alterations in ROS balance led to defective auxin distribution and growth-related responses in roots.
Article
Plant Sciences
Samuel Daniel Lup, Carla Navarro-Quiles, Jose Luis Micol
Summary: Mapping-by-sequencing combines NGS with classical genetic mapping to establish gene-to-phenotype relationships. Easymap v.2 is a versatile tool that automates mapping of mutations and DNA insertions, and includes workflows for QTL-seq and variant density mapping analyses.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Taras Pasternak, Jose Manuel Perez-Perez, Benedetto Ruperti, Tatiana Aleksandrova, Klaus Palme
Summary: Arabidopsis thaliana research often relies on in vitro culture media for studying plant responses to various signals. This study describes an optimized and nutritionally balanced culture medium that enables the growth and propagation of A. thaliana mutants, even those lacking a root system. The use of this protocol can avoid bias in phenotypic characterization during stress experiments.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Taras Pasternak, Stefan Kircher, Klaus Palme, Jose Manuel Perez-Perez
Summary: Root development is regulated by sucrose and light. Carbohydrate starvation during skotomorphogenesis leads to compaction of nuclei in the root apical meristem, hindering cell cycle progression and causing irreversible root differentiation. Adding carbohydrates promotes seedling growth. Transferring carbohydrate-grown seedlings from light to dark promotes cell elongation and reduces root meristem size. Carbohydrates are essential for cell cycle activity and auxin-regulated response in the root apical meristem.
Article
Plant Sciences
Olha Yaroshko, Taras Pasternak, Eduardo Larriba, Jose Manuel Perez-Perez
Summary: This study analyzed the effect of different concentrations of zeatin and indole-3-acetic acid on the regeneration of cotyledon explants in tomato cultivars M82 and Micro-Tom. The best hormone combination produced shoot-like structures after 2-3 weeks, and leaf primordia were observed from these structures after about 3-4 weeks. Whole plantlets could be obtained between 4 and 6 weeks by transferring the regenerating micro-stems to a defined growth medium. This method has direct applications for plant transformation, gene editing techniques, and vegetative propagation of elite cultivars.
Article
Plant Sciences
Riad Nadi, Lucia Juan-Vicente, Eduardo Mateo-Bonmati, Jose Luis Micol
Summary: This study confirms the unequal functional redundancy between the paralogous genes ICU11 and CP2 in Arabidopsis, independent of allele or genetic background. Additionally, it is shown that an increase in sucrose content in the culture medium can partially rescue the growth of icu11 cp2 double mutants.
FRONTIERS IN PLANT SCIENCE
(2023)
