期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 168, 期 13, 页码 1517-1525出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2011.02.013
关键词
Broccoli; Post-harvest; Proteases; Senescence; Zymography
资金
- University of Basilicata (Fondo Ateneo)
- Italian Foundation for Multiple Sclerosis (FISM)
Zymographic analysis of Broccoli florets (Brassica oleracea L var. Italica) revealed the presence of acidic metallo-proteases, serine proteases and cysteine proteases. Under conditions which were denaturing for the other proteases, the study was restricted to cysteine proteases. 2-D zymography, a technique that combines IEF and zymography was used to show the presence of 11 different cysteine protease spots with molecular mass of 44 and 47-48 kDa and pis ranging between 4.1 and 4.7. pI differences could be ascribed to different degrees of phosphorylation that partly disappeared in the presence of alkaline phosphatase. Post-harvest senescence of Broccoli florets was characterized by decrease in protein and chlorophyll contents and increase of protease activity. In particular, as determined by 2-D zymography, the presence of cysteine protease clearly increased during senescence, a finding that may represent a useful tool for the control of the aging process. (C) 2011 Elsevier GmbH. All rights reserved.
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